##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146517_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3229929 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.898282593827915 31.0 31.0 34.0 27.0 34.0 2 30.91969328118358 31.0 31.0 34.0 27.0 34.0 3 31.144990803203413 31.0 31.0 34.0 27.0 34.0 4 34.63736880903574 37.0 35.0 37.0 31.0 37.0 5 34.66730197474929 37.0 35.0 37.0 31.0 37.0 6 34.66048386822125 37.0 35.0 37.0 31.0 37.0 7 34.66574776101889 37.0 35.0 37.0 31.0 37.0 8 34.61794330463611 37.0 35.0 37.0 31.0 37.0 9 36.17641842901191 39.0 37.0 39.0 30.0 39.0 10-11 36.177854219086555 39.0 37.0 39.0 31.0 39.0 12-13 36.11964535443349 39.0 37.0 39.0 30.0 39.0 14-15 37.37970215444364 40.0 38.0 41.0 31.0 41.0 16-17 37.3930733461943 40.0 38.0 41.0 31.0 41.0 18-19 37.40633633123205 40.0 38.0 41.0 31.0 41.0 20-21 37.51667699197103 40.0 38.0 41.0 31.0 41.0 22-23 37.53076693017091 40.0 38.0 41.0 31.0 41.0 24-25 37.55228969429359 40.0 38.0 41.0 31.0 41.0 26-27 37.61439632264362 40.0 38.0 41.0 31.0 41.0 28-29 37.60067187235386 40.0 38.0 41.0 30.5 41.0 30-31 37.567983847322964 40.0 37.0 41.0 30.0 41.0 32-33 37.53131678745879 40.0 37.0 41.0 30.0 41.0 34-35 37.477112809600456 40.0 37.0 41.0 30.0 41.0 36-37 37.39363899330295 40.0 37.0 41.0 30.0 41.0 38-39 37.333739689014834 40.0 37.0 41.0 30.0 41.0 40-41 37.29048889309951 40.0 37.0 41.0 30.0 41.0 42-43 37.126896442615305 40.0 37.0 41.0 30.0 41.0 44-45 37.00050093980394 40.0 36.0 41.0 30.0 41.0 46-47 36.89102283672489 40.0 36.0 41.0 30.0 41.0 48-49 36.78620087314613 40.0 36.0 41.0 29.0 41.0 50-51 36.66425794498888 39.5 35.0 41.0 29.0 41.0 52-53 36.55251787268389 39.0 35.0 41.0 29.0 41.0 54-55 36.44952056221669 39.0 35.0 41.0 29.0 41.0 56-57 36.26803623237539 39.0 35.0 41.0 28.0 41.0 58-59 36.09600195546094 39.0 35.0 40.0 28.0 41.0 60-61 35.89007111301827 38.0 35.0 40.0 28.0 41.0 62-63 35.67844153849822 38.0 34.0 40.0 28.0 41.0 64-65 35.40270854250976 37.5 34.0 40.0 28.0 41.0 66-67 35.17322718239318 37.0 34.0 40.0 28.0 41.0 68-69 34.91236664954555 36.5 34.0 39.5 27.0 41.0 70-71 34.62138053189405 36.0 34.0 39.0 27.0 41.0 72-73 34.2792692966316 35.5 33.5 39.0 26.0 40.5 74-75 33.94401223680149 35.0 33.0 38.5 26.0 40.0 76-77 33.660376280717 35.0 33.0 37.0 26.0 39.0 78-79 33.330092085615505 35.0 33.0 37.0 26.0 39.0 80-81 33.07293813579184 35.0 33.0 36.5 26.0 39.0 82-83 32.79142931624813 35.0 33.0 36.0 26.0 37.5 84-85 32.573922367952974 35.0 32.5 36.0 25.0 37.0 86-87 32.3679354871268 35.0 32.0 35.0 25.0 37.0 88-89 32.17092976347158 35.0 32.0 35.0 25.0 36.0 90-91 32.014804504990664 35.0 32.0 35.0 25.0 36.0 92-93 31.853143211507124 35.0 32.0 35.0 24.5 36.0 94-95 31.725805273119008 35.0 32.0 35.0 24.0 35.5 96-97 31.638933549313315 35.0 32.0 35.0 24.0 35.0 98-99 31.534234498653063 35.0 32.0 35.0 23.5 35.0 100 30.320398993290564 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4704650293865704 1101 2 0.4198509753918245 1101 3 0.44501384333366545 1101 4 0.3931799143015766 1101 5 0.45637577500497173 1101 6 0.40819034501473084 1101 7 0.5217578420899329 1101 8 0.4916289049320923 1101 9 0.6384081410554927 1101 10-11 0.6221685358310225 1101 12-13 0.5625573847616252 1101 14-15 0.6571626109419952 1101 16-17 0.661549634128562 1101 18-19 0.6217390426120915 1101 20-21 0.6079311845406608 1101 22-23 0.668365738547557 1101 24-25 0.6114802628941263 1101 26-27 0.6642536595834372 1101 28-29 0.5883230380620077 1101 30-31 0.6538007256835456 1101 32-33 0.7223318405194732 1101 34-35 0.6224970325266952 1101 36-37 0.6190727842015562 1101 38-39 0.644132648632116 1101 40-41 0.6101612966155301 1101 42-43 0.6380972544481125 1101 44-45 0.5636359142700798 1101 46-47 0.5448942465566589 1101 48-49 0.6185127132570827 1101 50-51 0.6083524771580144 1101 52-53 0.5276464024557797 1101 54-55 0.5109538561334546 1101 56-57 0.5874164338808114 1101 58-59 0.6111317003485013 1101 60-61 0.5009577947402164 1101 62-63 0.46435945212570573 1101 64-65 0.5043297558715807 1101 66-67 0.43428175764808685 1101 68-69 0.45791761635151573 1101 70-71 0.40197794743772164 1101 72-73 0.4139918773929594 1101 74-75 0.4334262682581951 1101 76-77 0.35418343339691205 1101 78-79 0.4100121654975979 1101 80-81 0.3461266503143605 1101 82-83 0.35160590952914816 1101 84-85 0.4265025523270438 1101 86-87 0.39641145500389285 1101 88-89 0.3041860736734847 1101 90-91 0.31498851486072965 1101 92-93 0.39788575493148315 1101 94-95 0.38719680687895064 1101 96-97 0.3146525735973107 1101 98-99 0.35294643788225244 1101 100 0.40835205573913314 1102 1 0.46444920612936613 1102 2 0.4274674968014516 1102 3 0.44246696492471926 1102 4 0.4606342989973413 1102 5 0.4306084388928113 1102 6 0.4214161996198982 1102 7 0.4638433427961246 1102 8 0.5173824617201319 1102 9 0.5891275366024757 1102 10-11 0.5598193907232556 1102 12-13 0.5725577292447284 1102 14-15 0.6496735482805178 1102 16-17 0.6626037524240616 1102 18-19 0.6583639119955791 1102 20-21 0.5995550778886951 1102 22-23 0.634712610066849 1102 24-25 0.6789863200553583 1102 26-27 0.6847492559411066 1102 28-29 0.6346715171691102 1102 30-31 0.5788165144924378 1102 32-33 0.6127075567572646 1102 34-35 0.5768530213654373 1102 36-37 0.5303304920109895 1102 38-39 0.5643210808895205 1102 40-41 0.5754844791800906 1102 42-43 0.627436076545564 1102 44-45 0.5029546415198354 1102 46-47 0.5690597362393888 1102 48-49 0.5014872100246848 1102 50-51 0.5919935487301231 1102 52-53 0.5661125340253648 1102 54-55 0.4625379042692259 1102 56-57 0.4890636372521513 1102 58-59 0.5263859862924392 1102 60-61 0.43957722129068344 1102 62-63 0.5152528116397548 1102 64-65 0.49712144693913274 1102 66-67 0.5128468706959595 1102 68-69 0.501296651109854 1102 70-71 0.5211857511282858 1102 72-73 0.4871842023094217 1102 74-75 0.4793263451927601 1102 76-77 0.47832423512392097 1102 78-79 0.415301703545758 1102 80-81 0.42816185648749894 1102 82-83 0.4656257976869682 1102 84-85 0.4638910259224076 1102 86-87 0.39277543585063057 1102 88-89 0.34284224508827776 1102 90-91 0.3793815930469151 1102 92-93 0.4465462897989134 1102 94-95 0.46874342118418255 1102 96-97 0.48884791848231757 1102 98-99 0.5229574613415515 1102 100 0.46958507559284257 1103 1 0.3836219146953539 1103 2 0.33302963435835053 1103 3 0.4359649553672753 1103 4 0.384050036468075 1103 5 0.40445017986680654 1103 6 0.47567708495891026 1103 7 0.5278430706980828 1103 8 0.5070257298444147 1103 9 0.6079958354795068 1103 10-11 0.618175918525445 1103 12-13 0.6043280104693949 1103 14-15 0.7089682474704659 1103 16-17 0.7397492171731912 1103 18-19 0.7380333695567884 1103 20-21 0.6363860993334214 1103 22-23 0.6740052685214408 1103 24-25 0.7404096195842911 1103 26-27 0.7240475831049906 1103 28-29 0.773712201495691 1103 30-31 0.7992726188420392 1103 32-33 0.7759309549217264 1103 34-35 0.7320821222514624 1103 36-37 0.7925153826332405 1103 38-39 0.7935140834347649 1103 40-41 0.6932717551952194 1103 42-43 0.7924451181368823 1103 44-45 0.7139638418350174 1103 46-47 0.694647156239732 1103 48-49 0.6434699544735949 1103 50-51 0.6889157230202514 1103 52-53 0.6602981389049845 1103 54-55 0.6378909231403611 1103 56-57 0.717008106575932 1103 58-59 0.6344188307940755 1103 60-61 0.6533343284349584 1103 62-63 0.7273234777697795 1103 64-65 0.6027110777361031 1103 66-67 0.5860299076502784 1103 68-69 0.6192199761479813 1103 70-71 0.5203878802954947 1103 72-73 0.6231652007958743 1103 74-75 0.5657288122150419 1103 76-77 0.5250528229917464 1103 78-79 0.5090012166036288 1103 80-81 0.539576086081567 1103 82-83 0.5549958340233943 1103 84-85 0.5218053943998484 1103 86-87 0.43995185175249674 1103 88-89 0.5138261062729228 1103 90-91 0.51925274328471 1103 92-93 0.46184755335421457 1103 94-95 0.42102564354497574 1103 96-97 0.47777267770388576 1103 98-99 0.41634746287210334 1103 100 0.3702636200950451 1104 1 0.3521560656230598 1104 2 0.2879586253176569 1104 3 0.3885188232022827 1104 4 0.33719347649695663 1104 5 0.32753491889138786 1104 6 0.44879216743473194 1104 7 0.4922600671376145 1104 8 0.494404916799013 1104 9 0.678798054172681 1104 10-11 0.6114989024341142 1104 12-13 0.5283446278215891 1104 14-15 0.6757788431945926 1104 16-17 0.6425519651308917 1104 18-19 0.6321967667061799 1104 20-21 0.680531311686245 1104 22-23 0.651571926299205 1104 24-25 0.6762291503702826 1104 26-27 0.6375107055677631 1104 28-29 0.6782149643175686 1104 30-31 0.6758286977115233 1104 32-33 0.6823975324033498 1104 34-35 0.6762160407911608 1104 36-37 0.6992592643880329 1104 38-39 0.6267348948707294 1104 40-41 0.6201634157085607 1104 42-43 0.6784871675653079 1104 44-45 0.6350281583895878 1104 46-47 0.5950107966732077 1104 48-49 0.6082774939309488 1104 50-51 0.6205266666049241 1104 52-53 0.6052687800781626 1104 54-55 0.5454844770277134 1104 56-57 0.6527904538002858 1104 58-59 0.6131978160509846 1104 60-61 0.6104301405762058 1104 62-63 0.5781653432043186 1104 64-65 0.5639292472892592 1104 66-67 0.4984372990763646 1104 68-69 0.4970148827215084 1104 70-71 0.5212616939941981 1104 72-73 0.6005144457337082 1104 74-75 0.5305019198792991 1104 76-77 0.5626704009747812 1104 78-79 0.6030191585045941 1104 80-81 0.505567209754922 1104 82-83 0.4704446465497014 1104 84-85 0.49094417131411205 1104 86-87 0.5152819784198712 1104 88-89 0.4886319308466156 1104 90-91 0.4878747195651201 1104 92-93 0.5033953968047662 1104 94-95 0.5015854485403146 1104 96-97 0.47645592176429474 1104 98-99 0.5137855987214159 1104 100 0.44802147722673524 1105 1 0.2264764484788273 1105 2 0.25388776397985424 1105 3 0.2640523901888905 1105 4 0.4019145702672944 1105 5 0.2594572680928309 1105 6 0.23740933311341195 1105 7 0.29050757315665976 1105 8 0.361340712326907 1105 9 0.3676002729210808 1105 10-11 0.3454256627482337 1105 12-13 0.39554210638415555 1105 14-15 0.46903966874521785 1105 16-17 0.3950398761165772 1105 18-19 0.39931653619849783 1105 20-21 0.45179275475487657 1105 22-23 0.44849368401605005 1105 24-25 0.5177669212813569 1105 26-27 0.45423687814718505 1105 28-29 0.42718296871060346 1105 30-31 0.41213535731543516 1105 32-33 0.459086672786718 1105 34-35 0.43182890583056377 1105 36-37 0.41109188328621826 1105 38-39 0.39472341157170376 1105 40-41 0.4473890805014946 1105 42-43 0.4644169563884404 1105 44-45 0.3998401937588909 1105 46-47 0.3866791325808805 1105 48-49 0.33818438467923073 1105 50-51 0.4560664330514683 1105 52-53 0.40013167233124847 1105 54-55 0.37069450801287473 1105 56-57 0.3356399905066567 1105 58-59 0.3350262547650118 1105 60-61 0.4116609680786638 1105 62-63 0.3605364450881865 1105 64-65 0.448201341531842 1105 66-67 0.34274911248718354 1105 68-69 0.33716917319696194 1105 70-71 0.26931913584829204 1105 72-73 0.21911978576720514 1105 74-75 0.2723023719974407 1105 76-77 0.3149642078950592 1105 78-79 0.33170369272699674 1105 80-81 0.20526407558800486 1105 82-83 0.25896867195238116 1105 84-85 0.267754913375704 1105 86-87 0.26788353268221243 1105 88-89 0.2375468567478265 1105 90-91 0.22724779176036236 1105 92-93 0.24768284941348995 1105 94-95 0.23679641160267906 1105 96-97 0.2946017248068067 1105 98-99 0.19349311802154645 1105 100 0.2759214479425154 1106 1 0.025550126937421425 1106 2 0.12762311193108644 1106 3 0.12016471985440447 1106 4 0.09632517087148784 1106 5 0.09642750409779666 1106 6 0.1282401582152275 1106 7 0.20047547149249567 1106 8 0.22181698169551112 1106 9 0.2832272488883021 1106 10-11 0.21196640919054488 1106 12-13 0.29325157641471833 1106 14-15 0.26969095018266387 1106 16-17 0.2855892930997257 1106 18-19 0.26354062003010625 1106 20-21 0.22463457247692986 1106 22-23 0.23190151783676782 1106 24-25 0.31539187639477717 1106 26-27 0.23089957074989798 1106 28-29 0.20973792454538653 1106 30-31 0.25682304582915094 1106 32-33 0.2819528131541418 1106 34-35 0.22917232070882676 1106 36-37 0.23281931164671477 1106 38-39 0.19502194198716438 1106 40-41 0.17012393671515724 1106 42-43 0.15515029273403513 1106 44-45 0.22220633927885558 1106 46-47 0.1532938378329618 1106 48-49 0.18432680783829625 1106 50-51 0.17504468905570292 1106 52-53 0.09777285134935454 1106 54-55 0.04578637521255757 1106 56-57 0.1305399770043536 1106 58-59 0.12266696873308547 1106 60-61 0.14056407886705102 1106 62-63 0.009164190766057345 1106 64-65 0.07104272326948546 1106 66-67 0.1332471757915883 1106 68-69 0.15503100524572488 1106 70-71 0.11786548455813772 1106 72-73 0.10663006112703499 1106 74-75 0.16048505422109116 1106 76-77 0.14875347522722393 1106 78-79 0.09263342551611942 1106 80-81 0.07300661966007738 1106 82-83 0.1625520410232255 1106 84-85 0.15625157043222515 1106 86-87 0.12571502814308388 1106 88-89 0.08384887662396068 1106 90-91 0.13353115943077398 1106 92-93 0.16634857065457354 1106 94-95 0.12174673183983131 1106 96-97 0.18334689297656226 1106 98-99 0.16496546907936604 1106 100 0.27705276142613755 1107 1 0.02156010334894276 1107 2 0.0693800797787496 1107 3 0.0368387640685377 1107 4 0.01042518877434162 1107 5 0.08195300188551613 1107 6 0.15845244468441422 1107 7 0.0894996973910338 1107 8 0.1427198361178128 1107 9 0.20522394768173058 1107 10-11 0.2503580595774153 1107 12-13 0.1469566545281893 1107 14-15 0.2240212262603336 1107 16-17 0.18573074527649425 1107 18-19 0.18836980521442115 1107 20-21 0.2648178602661986 1107 22-23 0.2694522507715149 1107 24-25 0.273013891193159 1107 26-27 0.26369535138294964 1107 28-29 0.20596836666705798 1107 30-31 0.25353130710843885 1107 32-33 0.2474024058151585 1107 34-35 0.23206173717433387 1107 36-37 0.2198240077015896 1107 38-39 0.2580109504251098 1107 40-41 0.23951825482320288 1107 42-43 0.3034855983317186 1107 44-45 0.2230219271513505 1107 46-47 0.1714049791100436 1107 48-49 0.21487452679898666 1107 50-51 0.27096350357235366 1107 52-53 0.22101588842005526 1107 54-55 0.19371603865867826 1107 56-57 0.17840907078576862 1107 58-59 0.15556505135768361 1107 60-61 0.2637573174923489 1107 62-63 0.26756844692237536 1107 64-65 0.2176068026794411 1107 66-67 0.14093519504797314 1107 68-69 0.21962303178914766 1107 70-71 0.18922247621068777 1107 72-73 0.23548667406548418 1107 74-75 0.2885848537485387 1107 76-77 0.2322515737873232 1107 78-79 0.17204817451227683 1107 80-81 0.19818146753505061 1107 82-83 0.22028872750106387 1107 84-85 0.17419474954530045 1107 86-87 0.16844356980852382 1107 88-89 0.16570092138501025 1107 90-91 0.14762903784485104 1107 92-93 0.1106645638003414 1107 94-95 0.22905721740049856 1107 96-97 0.2132317392973313 1107 98-99 0.2601247650842744 1107 100 0.2937319207398481 1108 1 0.12340513398031305 1108 2 0.052825056832535466 1108 3 0.12743713261203737 1108 4 0.1148296416037411 1108 5 0.042357241924506184 1108 6 0.0974448868248956 1108 7 0.1297981881940231 1108 8 0.18310441679245315 1108 9 0.1813800218505861 1108 10-11 0.1852898502752467 1108 12-13 0.14348248291462085 1108 14-15 0.15318469869589535 1108 16-17 0.16031131542181498 1108 18-19 0.1547270708196038 1108 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16-17 -0.9619367519839912 2215 18-19 -0.903282824724279 2215 20-21 -0.773704215653261 2215 22-23 -0.708678429137656 2215 24-25 -0.7399048816820866 2215 26-27 -0.7481176182739375 2215 28-29 -0.8005760741592169 2215 30-31 -0.8669353088385705 2215 32-33 -0.8152216307421654 2215 34-35 -0.7495199181160714 2215 36-37 -0.7370107108406785 2215 38-39 -0.7566759990686833 2215 40-41 -0.7500954232303485 2215 42-43 -0.8168739062606036 2215 44-45 -0.7460256714141735 2215 46-47 -0.6678810249223446 2215 48-49 -0.7072142544213662 2215 50-51 -0.7260643574093493 2215 52-53 -0.6722891203768526 2215 54-55 -0.6658196065174593 2215 56-57 -0.6534617187203793 2215 58-59 -0.6051498782936235 2215 60-61 -0.6393610741601918 2215 62-63 -0.6261414553842357 2215 64-65 -0.5153171016095257 2215 66-67 -0.5751469961139364 2215 68-69 -0.6315364040392879 2215 70-71 -0.5380294744538858 2215 72-73 -0.5329459731397179 2215 74-75 -0.4995710573015515 2215 76-77 -0.5610926811974295 2215 78-79 -0.5474239878655922 2215 80-81 -0.48572589593521087 2215 82-83 -0.46997041273780127 2215 84-85 -0.5159083571106109 2215 86-87 -0.5360527781244073 2215 88-89 -0.4865245084661751 2215 90-91 -0.5278087828772442 2215 92-93 -0.551744120535119 2215 94-95 -0.4940972606024765 2215 96-97 -0.5585138414763087 2215 98-99 -0.5308194332220317 2215 100 -0.46019815327504077 2216 1 -0.5190901825840832 2216 2 -0.4022748826981051 2216 3 -0.5194468568044641 2216 4 -0.45539473545786535 2216 5 -0.504082264948579 2216 6 -0.4783015566118962 2216 7 -0.5697889447750271 2216 8 -0.5274550686139463 2216 9 -0.8285902023426175 2216 10-11 -0.8589885865548368 2216 12-13 -0.7287508006512837 2216 14-15 -0.8655317881043985 2216 16-17 -0.8312176883635871 2216 18-19 -0.9107624485077821 2216 20-21 -0.8219835977914727 2216 22-23 -0.8970175850528506 2216 24-25 -0.8470558471516298 2216 26-27 -0.9166154171962049 2216 28-29 -0.9516502888213125 2216 30-31 -0.9964953156214733 2216 32-33 -0.9548233789346199 2216 34-35 -0.9459116274164572 2216 36-37 -0.8641430635852885 2216 38-39 -0.848816347718369 2216 40-41 -0.9196888965062016 2216 42-43 -0.8909365755985164 2216 44-45 -0.8384538807683128 2216 46-47 -0.8073171766465421 2216 48-49 -0.6996817555011248 2216 50-51 -0.865174476810374 2216 52-53 -0.8492203259492541 2216 54-55 -0.7663648853836378 2216 56-57 -0.6684947038380855 2216 58-59 -0.711198638272684 2216 60-61 -0.8372498858523301 2216 62-63 -0.8216025284378432 2216 64-65 -0.7643474392818206 2216 66-67 -0.7101164424065232 2216 68-69 -0.793788541049274 2216 70-71 -0.71402997609934 2216 72-73 -0.6857554228894074 2216 74-75 -0.7271973873396576 2216 76-77 -0.7424511346594045 2216 78-79 -0.6145478149385539 2216 80-81 -0.5644711027829175 2216 82-83 -0.6337740331601722 2216 84-85 -0.5769276692763157 2216 86-87 -0.6136344612430555 2216 88-89 -0.6431894439867634 2216 90-91 -0.6178313342499635 2216 92-93 -0.6299556692026975 2216 94-95 -0.6731175332175461 2216 96-97 -0.681563100021382 2216 98-99 -0.7075097312114096 2216 100 -0.8871838498483697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 984.0 1 257.0 2 172.0 3 185.0 4 139.0 5 194.0 6 250.0 7 499.0 8 1640.0 9 2278.0 10 2666.0 11 3562.0 12 5214.0 13 7879.0 14 10466.0 15 11459.0 16 12372.0 17 13597.0 18 14884.0 19 16310.0 20 17748.0 21 19562.0 22 21484.0 23 24623.0 24 28941.0 25 35112.0 26 37293.0 27 35540.0 28 37317.0 29 44481.0 30 51650.0 31 60200.0 32 75584.0 33 99630.0 34 144016.0 35 217626.0 36 386392.0 37 732051.0 38 835174.0 39 220272.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21566480046658 20.20259210295524 13.411910831460006 24.169832265118174 2 14.798016505165377 21.311416496119072 40.36203179961324 23.52853519910231 3 17.912126524716157 26.368611498945565 30.772076662942215 24.947185313396062 4 12.007632530469543 17.904592749405918 40.15751544896427 29.93025927116027 5 12.768407343632978 38.88745491103674 35.60209975672643 12.742037988603853 6 29.38006510103552 39.010667545919034 18.65385759582322 12.955409757222224 7 25.831265447171603 33.38073358106651 23.49262254239683 17.29537842936506 8 24.724526080118995 35.873817621220454 21.167837747805677 18.233818550854878 9 25.86394452016405 16.091271679363082 20.882334624611666 37.1624491758612 10-11 23.71020876609373 27.022003232379323 29.397873297671396 19.869914703855553 12-13 24.5648257002361 25.044525518385164 28.463242610282087 21.927406171096653 14-15 22.0790842114314 26.483256942739203 26.19613572298914 25.24152312284026 16-17 21.717343623806926 28.98628165802508 26.996783555651334 22.29959116251666 18-19 21.582416826512148 29.046329394635293 27.973896733079034 21.397357045773518 20-21 22.910585680138432 27.98780213873092 27.309700816237374 21.791911364893274 22-23 22.67047023981318 27.915627353317586 27.02949024033061 22.384412166538624 24-25 22.463072533748445 27.993191440313716 27.407256845121537 22.136479180816306 26-27 22.301510643752824 28.10689684819746 27.238789842909473 22.35280266514025 28-29 22.051576572249775 28.67607096666644 27.07960603820262 22.192746422881164 30-31 22.532435106933324 28.04914949594452 27.08539621889055 22.33301917823161 32-33 22.392680287990395 28.36984910546442 26.8332320304643 22.404238576080882 34-35 22.829061626092923 28.178811933376647 27.125657311369324 21.86646912916111 36-37 22.633430640671286 27.88759305876044 27.2268834129388 22.25209288762947 38-39 22.409531140475647 28.124676559378898 27.317570193199796 22.14822210694566 40-41 22.573664558973245 27.88456547744748 27.388755790527576 22.153014173051698 42-43 22.293966621887748 27.892679344213526 27.11090761752916 22.70244641636956 44-45 22.30137990637423 28.113977149164437 27.198930433890705 22.385712510570627 46-47 22.41477506007539 28.038745721904007 26.989165513704627 22.557313704315977 48-49 22.442414564314184 27.757932455938377 27.23428763386295 22.565365345884487 50-51 22.28717038454697 27.813960132853303 27.22626585906865 22.672603623531074 52-53 22.242765237189957 28.055785958587578 27.01213124259433 22.68931756162814 54-55 22.125661738001952 27.887275542064287 27.108234369596556 22.878828350337205 56-57 22.480303412742572 27.66414674927868 27.072555739788584 22.782994098190162 58-59 22.33751140848497 27.68275656888556 27.331336493360997 22.648395529268477 60-61 22.29537830575634 27.879895172158175 27.14399913599 22.68072738609548 62-63 22.2005304069785 27.655757658399622 27.114255985285585 23.029455949336295 64-65 21.76197171793304 27.78659308911959 27.207380646968787 23.24405454597858 66-67 22.216029025688798 27.518944518153297 26.917780899686022 23.34724555647188 68-69 21.992009610904343 27.481632843434024 27.166207252092388 23.360150293569244 70-71 22.023459297212824 27.30916948757265 27.18382286285864 23.483548352355886 72-73 22.200418377624544 27.19087316959789 27.460106918726275 23.14860153405129 74-75 22.007723563314485 27.65673175904379 27.249649362126522 23.085895315515202 76-77 21.92410625254807 27.290782542023862 27.354423245855646 23.430687959572424 78-79 21.94706187272441 27.277586178842263 27.374238823617837 23.40111312481549 80-81 21.98409949707551 27.328363748216983 27.458546113550447 23.22899064115706 82-83 22.111126762724183 26.915319659402304 27.911692049398972 23.061861528474545 84-85 22.184434147120804 27.055645372612 27.466539681378705 23.293380798888496 86-87 21.763272541607556 27.301065863169416 27.591685384030722 23.3439762111923 88-89 22.10960808565644 26.776004718058505 27.43198963911377 23.682397557171285 90-91 22.09174037842317 26.737270040083576 27.579803009708197 23.591186571785055 92-93 22.272013184025525 26.782685926012466 27.56345765200451 23.381843237957497 94-95 21.690728154000958 26.66635367674835 27.815243600673206 23.827674568577486 96-97 21.736908021045522 26.573219839017888 27.846087602294944 23.843784537641643 98-99 21.615306693121852 26.827561060234363 27.5988245789632 23.958307667680586 100 22.02189303017042 26.50931245208766 27.296486997327772 24.17230752041415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1808.0 1 1277.0 2 758.5 3 705.5 4 619.0 5 554.5 6 494.0 7 462.5 8 437.0 9 426.5 10 405.0 11 391.0 12 419.5 13 448.5 14 484.0 15 550.5 16 620.0 17 724.0 18 942.0 19 1206.5 20 1600.0 21 2193.0 22 3147.5 23 4508.0 24 6335.0 25 8882.0 26 12851.0 27 17882.5 28 23036.5 29 29788.0 30 37464.0 31 45375.0 32 54902.5 33 63355.0 34 71313.0 35 79202.0 36 86776.0 37 96394.0 38 101419.5 39 102030.0 40 104220.0 41 105640.0 42 106828.0 43 110890.0 44 117138.0 45 125272.5 46 131794.5 47 137538.0 48 145064.5 49 142857.0 50 131663.5 51 124693.0 52 119535.5 53 113496.5 54 106671.5 55 95559.5 56 85037.5 57 74494.5 58 63142.0 59 53837.5 60 43232.0 61 32975.5 62 25881.0 63 19848.0 64 14015.0 65 9717.5 66 7080.0 67 5097.0 68 3608.5 69 2638.5 70 1999.5 71 1589.5 72 1214.5 73 1013.0 74 796.0 75 607.0 76 471.5 77 352.5 78 274.5 79 207.5 80 156.5 81 121.0 82 92.5 83 61.5 84 30.0 85 26.0 86 27.0 87 22.5 88 16.5 89 14.0 90 15.0 91 12.5 92 10.0 93 7.5 94 5.0 95 7.5 96 6.5 97 4.5 98 3.5 99 2.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09446028070586071 2 0.06473826514452793 3 0.0807138485087443 4 0.08328356443748454 5 0.08353124790049564 6 0.09117847482096356 7 0.08672017248676364 8 0.08836107542921222 9 0.0736239093800514 10-11 0.08003271898546377 12-13 0.07076006933898547 14-15 0.07884074231972282 16-17 0.0807293287251825 18-19 0.08029588266491307 20-21 0.07164244167596254 22-23 0.0754660551361965 24-25 0.07805125128137491 26-27 0.07218424925129932 28-29 0.08083769024024987 30-31 0.08528051235801158 32-33 0.08692141530046016 34-35 0.08862423910866152 36-37 0.08881000170591985 38-39 0.09000197837166081 40-41 0.08617836491142684 42-43 0.09176672304561494 44-45 0.08749418330867334 46-47 0.08464582348404562 48-49 0.0937481907497038 50-51 0.09012582010316636 52-53 0.09583801996886 54-55 0.0873393811442914 56-57 0.09404231486202948 58-59 0.0979588096208926 60-61 0.09642626819351137 62-63 0.09788140853870161 64-65 0.09969259386197035 66-67 0.10124061550578975 68-69 0.09837677546472383 70-71 0.10144185831948628 72-73 0.09842321611403843 74-75 0.10246355260440711 76-77 0.09970807407840854 78-79 0.10608592325094453 80-81 0.10221586914139599 82-83 0.10602400238519175 84-85 0.10243259217153071 86-87 0.09911982585375716 88-89 0.09839225568116204 90-91 0.09817553265102733 92-93 0.09268005581546838 94-95 0.09250977343464825 96-97 0.0905437859469976 98-99 0.10342332602357512 100 0.24455645929059122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3229929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.20892650479016 #Duplication Level Percentage of deduplicated Percentage of total 1 70.60987865081421 41.8073511554821 2 15.498502684150678 18.352994127203413 3 6.148691540396898 10.921722765479549 4 3.040772482500299 7.201634977365942 5 1.6349686367200287 4.840236892459657 6 0.9720365365246351 3.4531943910634744 7 0.5947135793266309 2.464864682682583 8 0.39592193348937127 1.875369012928528 9 0.27514949516422804 1.4662175613308004 >10 0.8192533061667362 6.997372404697838 >50 0.007729667007939936 0.3093848419606204 >100 0.002299483835574009 0.22643626454481391 >500 0.0 0.0 >1k 8.200390285448919E-5 0.08322092280070628 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.096043287638831E-5 2 0.0 0.0 0.0 0.0 3.096043287638831E-5 3 0.0 0.0 0.0 0.0 3.096043287638831E-5 4 0.0 0.0 0.0 0.0 3.096043287638831E-5 5 0.0 0.0 0.0 0.0 3.096043287638831E-5 6 0.0 0.0 0.0 0.0 3.096043287638831E-5 7 0.0 0.0 0.0 0.0 3.096043287638831E-5 8 3.096043287638831E-5 0.0 0.0 0.0 3.096043287638831E-5 9 3.096043287638831E-5 0.0 0.0 0.0 3.096043287638831E-5 10-11 3.096043287638831E-5 0.0 0.0 0.0 3.096043287638831E-5 12-13 3.096043287638831E-5 0.0 0.0 1.5480216438194154E-5 2.0124281369652397E-4 14-15 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 2.4768346301110646E-4 16-17 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 2.7864389588749475E-4 18-19 3.096043287638831E-5 0.0 0.0 9.288129862916492E-5 2.7864389588749475E-4 20-21 3.096043287638831E-5 0.0 0.0 1.5480216438194154E-4 3.096043287638831E-4 22-23 3.096043287638831E-5 0.0 0.0 4.953669260222128E-4 3.7152519451665966E-4 24-25 3.096043287638831E-5 0.0 0.0 0.0011919766657409498 3.7152519451665966E-4 26-27 3.096043287638831E-5 0.0 0.0 0.0023220324657291227 3.7152519451665966E-4 28-29 3.096043287638831E-5 0.0 0.0 0.0077246280026588825 3.7152519451665966E-4 30-31 3.096043287638831E-5 0.0 0.0 0.021904506260044726 3.7152519451665966E-4 32-33 3.096043287638831E-5 0.0 0.0 0.05137883835836639 3.870054109548538E-4 34-35 3.096043287638831E-5 0.0 0.0 0.09794332940445441 4.0248562739304795E-4 36-37 3.096043287638831E-5 0.0 0.0 0.17138147618724747 4.0248562739304795E-4 38-39 3.096043287638831E-5 0.0 0.0 0.27788536528202323 5.10847142460407E-4 40-41 3.096043287638831E-5 0.0 0.0 0.40118528921223967 5.263273588986012E-4 42-43 3.096043287638831E-5 0.0 0.0 0.5228597904164457 5.572877917749895E-4 44-45 3.096043287638831E-5 0.0 0.0 0.675819809042242 5.727680082131836E-4 46-47 3.096043287638831E-5 0.0 0.0 0.8606845537471567 5.882482246513778E-4 48-49 3.096043287638831E-5 0.0 0.0 1.058924205454671 6.501690904041544E-4 50-51 3.096043287638831E-5 0.0 0.0 1.2743468974085808 6.811295232805427E-4 52-53 3.096043287638831E-5 0.0 0.0 1.481193549455731 7.430503890333193E-4 54-55 3.096043287638831E-5 0.0 0.0 1.7083347652533538 7.894910383479017E-4 56-57 3.096043287638831E-5 0.0 0.0 1.9871489435216687 8.204514712242901E-4 58-59 3.096043287638831E-5 0.0 0.0 2.301908184359471 8.359316876624842E-4 60-61 3.096043287638831E-5 0.0 0.0 2.6292218807286476 8.359316876624842E-4 62-63 3.096043287638831E-5 0.0 0.0 2.9645852896456857 8.359316876624842E-4 64-65 3.096043287638831E-5 0.0 0.0 3.310304963359876 8.359316876624842E-4 66-67 3.096043287638831E-5 0.0 0.0 3.70545296816122 8.514119041006784E-4 68-69 3.096043287638831E-5 0.0 0.0 4.150029304049717 8.668921205388726E-4 70-71 3.096043287638831E-5 0.0 0.0 4.627377258137873 8.823723369770667E-4 72-73 3.096043287638831E-5 0.0 0.0 5.119756502387514 9.288129862916492E-4 74-75 3.096043287638831E-5 0.0 0.0 5.609983996552247 9.597734191680374E-4 76-77 3.096043287638831E-5 0.0 0.0 6.149624341587694 9.597734191680374E-4 78-79 3.096043287638831E-5 0.0 0.0 6.753012217915626 9.597734191680374E-4 80-81 3.096043287638831E-5 0.0 0.0 7.398707525769142 9.597734191680374E-4 82-83 3.096043287638831E-5 0.0 0.0 8.068706773430623 0.001021694284920814 84-85 3.096043287638831E-5 0.0 0.0 8.723256765086788 0.001021694284920814 86-87 3.096043287638831E-5 0.0 0.0 9.404881655293352 0.0010526547177972024 88 3.096043287638831E-5 0.0 0.0 9.943190701715116 0.0010836151506735906 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1125 0.0 27.16562 1 GTACATG 10725 0.0 25.251314 1 TACATGG 10505 0.0 24.43439 2 ACATGGG 10675 0.0 23.29661 3 TATACGA 210 3.8504368E-8 22.386469 2 GTGATCG 780 0.0 22.291039 8 TGATCGC 860 0.0 20.216827 9 GCATCGT 1205 0.0 19.888697 8 CATGGGG 7135 0.0 19.832191 4 TAGGCAT 1920 0.0 19.097273 5 GGCGTCG 815 0.0 19.027403 8 GTATCAA 10320 0.0 18.998318 1 TACGGGC 845 0.0 18.915611 4 CGTTAAC 375 9.094947E-12 18.806969 1 GGTATCA 8085 0.0 18.37663 1 ATACGGG 860 0.0 18.03933 3 CTAGGCA 2225 0.0 17.747921 4 GTATAAG 1580 0.0 17.557137 1 CGCAATA 165 0.0015695391 17.095123 2 TACTAGG 1940 0.0 16.962944 2 >>END_MODULE