FastQCFastQC Report
Mon 16 Jan 2017
ERR1146517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229929
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102780.31821132910351896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94550.2927308928462514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.16046792359832057No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42250.1308078289027406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115000.035.3285681
GTATCAA174000.030.7541081
ATCAACG199650.026.1658423
CAACGCA201500.025.961025
TATCAAC207800.025.5024432
AACGCAG211250.025.0520536
TCAACGC209550.024.9969754
GAGTACT136450.023.09440212-13
ACGCAGA228750.022.9084267
CGCAGAG231000.022.6039098
GTACTAG10950.021.9011141
AGAGTAC183650.021.71412810-11
GTACTTT147350.021.16275814-15
GTACATG101800.021.0633371
GCAGAGT247450.020.7400579
ACATGGG99550.020.4992713
TAGTACT16350.020.4188164
CTGTGCG13650.020.3139389
TACATGG102650.020.2472822
AGTACTT145050.019.9592612-13
Produced by FastQC (version 0.11.5)