##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146517_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3229929 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.274117790205295 33.0 31.0 34.0 30.0 34.0 2 32.333081625014046 34.0 31.0 34.0 30.0 34.0 3 32.45206411657965 34.0 31.0 34.0 30.0 34.0 4 35.99664698511949 37.0 35.0 37.0 35.0 37.0 5 36.05649133463925 37.0 35.0 37.0 35.0 37.0 6 36.13532495605941 37.0 36.0 37.0 35.0 37.0 7 36.099396302519345 37.0 36.0 37.0 35.0 37.0 8 36.1020059574065 37.0 36.0 37.0 35.0 37.0 9 37.88256119561761 39.0 38.0 39.0 35.0 39.0 10-11 37.8359216255218 39.0 38.0 39.0 35.0 39.0 12-13 37.82014867819076 39.0 38.0 39.0 35.0 39.0 14-15 39.15740284074356 40.0 39.0 41.0 36.0 41.0 16-17 39.10593994480993 40.0 39.0 41.0 36.0 41.0 18-19 39.220885505532785 40.0 39.0 41.0 36.0 41.0 20-21 39.281260671674204 41.0 39.0 41.0 36.0 41.0 22-23 39.24912420675501 40.5 39.0 41.0 36.0 41.0 24-25 39.240405748857015 41.0 39.0 41.0 36.0 41.0 26-27 39.16019469777818 41.0 39.0 41.0 35.5 41.0 28-29 39.100088732600625 40.0 39.0 41.0 35.0 41.0 30-31 38.9503623763866 40.0 38.5 41.0 35.0 41.0 32-33 38.83453289530513 40.0 38.0 41.0 35.0 41.0 34-35 38.75908711925247 40.0 38.0 41.0 35.0 41.0 36-37 38.68197087304396 40.0 38.0 41.0 35.0 41.0 38-39 38.590951380045816 40.0 38.0 41.0 35.0 41.0 40-41 38.49747626031408 40.0 38.0 41.0 35.0 41.0 42-43 38.38919106271376 40.0 38.0 41.0 34.0 41.0 44-45 38.240539962333536 40.0 38.0 41.0 34.0 41.0 46-47 37.993789027560666 40.0 38.0 41.0 33.0 41.0 48-49 37.704516415066706 40.0 37.5 41.0 33.0 41.0 50-51 37.601813383514006 40.0 37.0 41.0 33.0 41.0 52-53 37.515588732755425 40.0 37.0 41.0 33.0 41.0 54-55 37.36875299735691 40.0 37.0 41.0 32.0 41.0 56-57 37.228044486426796 40.0 36.0 41.0 32.0 41.0 58-59 37.06620981451914 39.5 36.0 41.0 32.0 41.0 60-61 36.87474740156827 39.0 35.0 41.0 31.5 41.0 62-63 36.65496083660044 39.0 35.0 41.0 31.0 41.0 64-65 36.39493360380367 38.5 35.0 41.0 31.0 41.0 66-67 36.13434954762164 37.5 35.0 40.0 31.0 41.0 68-69 35.83852261148775 37.0 35.0 40.0 31.0 41.0 70-71 35.521036220920024 37.0 35.0 39.5 30.0 41.0 72-73 35.18768539494212 36.0 35.0 39.0 30.0 41.0 74-75 34.85735413998265 36.0 35.0 39.0 30.0 40.5 76-77 34.55148379422582 35.0 34.5 37.5 30.0 39.5 78-79 34.24524145886798 35.0 34.0 37.0 29.5 39.0 80-81 33.96568484941929 35.0 34.0 37.0 29.0 39.0 82-83 33.69934834480881 35.0 34.0 36.0 29.0 38.0 84-85 33.4762155762557 35.0 34.0 36.0 29.0 37.0 86-87 33.284989855814175 35.0 34.0 35.5 29.0 37.0 88-89 33.08055579549891 35.0 34.0 35.0 29.0 36.0 90-91 32.99051681940996 35.0 34.0 35.0 29.0 36.0 92-93 32.88594609974399 35.0 34.0 35.0 29.0 36.0 94-95 32.80791528234831 35.0 34.0 35.0 29.0 36.0 96-97 32.75471581573465 35.0 34.0 35.0 29.0 35.5 98-99 32.688971646125964 35.0 34.0 35.0 29.0 35.0 100 31.967440460765545 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2951150805431979 1101 2 0.2654917198041957 1101 3 0.2601498977972625 1101 4 0.17225273881569336 1101 5 0.20566127948757895 1101 6 0.19473250017752264 1101 7 0.2101209793761143 1101 8 0.19482722089436777 1101 9 0.2691553141774037 1101 10-11 0.2830585558173908 1101 12-13 0.3126673014396033 1101 14-15 0.4716820297848727 1101 16-17 0.42020263621113685 1101 18-19 0.40554237850015795 1101 20-21 0.4278991654541855 1101 22-23 0.36956521198276704 1101 24-25 0.3710694955573359 1101 26-27 0.3158218294302557 1101 28-29 0.3708568106335335 1101 30-31 0.46069471645960647 1101 32-33 0.38974187647045966 1101 34-35 0.3484040549948304 1101 36-37 0.3530603308626752 1101 38-39 0.3913618434204764 1101 40-41 0.4323114862430728 1101 42-43 0.399138060233696 1101 44-45 0.416192099220261 1101 46-47 0.49469911368660036 1101 48-49 0.6102961822453423 1101 50-51 0.5975846713101944 1101 52-53 0.5974698862696997 1101 54-55 0.5584922756584731 1101 56-57 0.5399589792289348 1101 58-59 0.5415543164258736 1101 60-61 0.48134726417843154 1101 62-63 0.410369549305841 1101 64-65 0.3900718393947855 1101 66-67 0.35819475594655614 1101 68-69 0.2915848917623052 1101 70-71 0.3367300373228943 1101 72-73 0.3310709952787647 1101 74-75 0.2982751643673396 1101 76-77 0.2756292673188625 1101 78-79 0.328600193545995 1101 80-81 0.2860932425427265 1101 82-83 0.3543979681314582 1101 84-85 0.3494227553107052 1101 86-87 0.2661629017890377 1101 88-89 0.4276994532235108 1101 90-91 0.32231377225668467 1101 92-93 0.32764943158925774 1101 94-95 0.4006012090104747 1101 96-97 0.35440727051965126 1101 98-99 0.37816930360131806 1101 100 0.3888882853830111 1102 1 0.20646924362149832 1102 2 0.19443289357295157 1102 3 0.2281210071476778 1102 4 0.1562537952305405 1102 5 0.12194499577129392 1102 6 0.1464704174326812 1102 7 0.16633258386358563 1102 8 0.152828989240966 1102 9 0.2040904939401713 1102 10-11 0.2280922577476474 1102 12-13 0.25301660730615083 1102 14-15 0.43292710654719 1102 16-17 0.5121773281858282 1102 18-19 0.37381778125831744 1102 20-21 0.3568363202534144 1102 22-23 0.3783920173612998 1102 24-25 0.3364426281718451 1102 26-27 0.27585030670010724 1102 28-29 0.304996808566635 1102 30-31 0.38964048333640733 1102 32-33 0.36660811368152224 1102 34-35 0.38331569507887764 1102 36-37 0.38479985570564423 1102 38-39 0.41531432771778753 1102 40-41 0.402731870456563 1102 42-43 0.4126618927230439 1102 44-45 0.3694061265722226 1102 46-47 0.3845494931921465 1102 48-49 0.46662720754189024 1102 50-51 0.4301044503816982 1102 52-53 0.4145642861227188 1102 54-55 0.3413507964480331 1102 56-57 0.3554383274668993 1102 58-59 0.37594234219321265 1102 60-61 0.26293693887155456 1102 62-63 0.3537991082526375 1102 64-65 0.3384929585055545 1102 66-67 0.28572010416155535 1102 68-69 0.3258684243216976 1102 70-71 0.3362483351170553 1102 72-73 0.3818150787814645 1102 74-75 0.3503690474957111 1102 76-77 0.27900060862123865 1102 78-79 0.32658726394686255 1102 80-81 0.32513523503299524 1102 82-83 0.29753643885613457 1102 84-85 0.294636162593072 1102 86-87 0.29714628622070194 1102 88-89 0.3393980994049173 1102 90-91 0.3160866660295838 1102 92-93 0.2852826030155242 1102 94-95 0.27855141971930664 1102 96-97 0.20084646351056534 1102 98-99 0.20594555034308115 1102 100 0.3023392022822051 1103 1 0.2500020966261971 1103 2 0.2310612586605103 1103 3 0.21837082716616152 1103 4 0.14391584945741442 1103 5 0.16890174918810885 1103 6 0.19121920858042785 1103 7 0.1840416316904836 1103 8 0.23137745953975752 1103 9 0.2815789156014432 1103 10-11 0.29685511863185354 1103 12-13 0.2912137876901397 1103 14-15 0.4494343880042777 1103 16-17 0.525393923899145 1103 18-19 0.4053003754864335 1103 20-21 0.38742325841345604 1103 22-23 0.41235467190448105 1103 24-25 0.4376220031399569 1103 26-27 0.4682987356173314 1103 28-29 0.42184166166203596 1103 30-31 0.44593360095645806 1103 32-33 0.4271286712557725 1103 34-35 0.4316712025133853 1103 36-37 0.4304613090493348 1103 38-39 0.4256880657213813 1103 40-41 0.46266996815109707 1103 42-43 0.5332325050003348 1103 44-45 0.5098909145908337 1103 46-47 0.543760862355505 1103 48-49 0.5563316977265202 1103 50-51 0.48322875950776023 1103 52-53 0.4177274259074224 1103 54-55 0.41776358052123186 1103 56-57 0.42416717609535937 1103 58-59 0.45359657360125993 1103 60-61 0.42554244772324523 1103 62-63 0.40223256043991285 1103 64-65 0.3935630520959421 1103 66-67 0.3759617045515711 1103 68-69 0.37111322753246156 1103 70-71 0.31910899257395897 1103 72-73 0.33138340656891074 1103 74-75 0.3542181011046921 1103 76-77 0.3158713110310032 1103 78-79 0.31488421361439123 1103 80-81 0.2843021048284058 1103 82-83 0.3245039061335149 1103 84-85 0.27758326786667453 1103 86-87 0.2566079288992782 1103 88-89 0.3671620203377657 1103 90-91 0.2644631476884669 1103 92-93 0.2619856250044492 1103 94-95 0.32152732203611123 1103 96-97 0.3297103974814348 1103 98-99 0.3417295611816584 1103 100 0.35199709567645954 1104 1 0.24345159251607384 1104 2 0.23707468229624595 1104 3 0.28831264414182556 1104 4 0.16841718043468035 1104 5 0.13198041489762602 1104 6 0.17544875362105472 1104 7 0.17445664371177827 1104 8 0.13951889285876717 1104 9 0.26531975579639067 1104 10-11 0.22998587092652656 1104 12-13 0.25622525863391843 1104 14-15 0.43092127538775316 1104 16-17 0.48957114648873556 1104 18-19 0.37564197587248316 1104 20-21 0.342383166301822 1104 22-23 0.3806162821247412 1104 24-25 0.32172641439607474 1104 26-27 0.3270000451539232 1104 28-29 0.37359044063989444 1104 30-31 0.44214205699785936 1104 32-33 0.48016357598307025 1104 34-35 0.44067996090709016 1104 36-37 0.3968090064295353 1104 38-39 0.41586915429142124 1104 40-41 0.4165772205088061 1104 42-43 0.4392418957920725 1104 44-45 0.4389405628778107 1104 46-47 0.5145762062909611 1104 48-49 0.5798024335697818 1104 50-51 0.5732786742769278 1104 52-53 0.5446726834724984 1104 54-55 0.5586194212401736 1104 56-57 0.5084480658998345 1104 58-59 0.48830150862761457 1104 60-61 0.46356807367196495 1104 62-63 0.4535248788248083 1104 64-65 0.5138904450315707 1104 66-67 0.4868342982224618 1104 68-69 0.45248762539230825 1104 70-71 0.4676052227435292 1104 72-73 0.47332571026075243 1104 74-75 0.41856548011220696 1104 76-77 0.4261802315061871 1104 78-79 0.449420282547905 1104 80-81 0.4332854442803793 1104 82-83 0.43247595075489187 1104 84-85 0.45179613950227093 1104 86-87 0.4436369428994382 1104 88-89 0.4574621148043576 1104 90-91 0.45945087757561254 1104 92-93 0.4452696901186002 1104 94-95 0.5011733641280856 1104 96-97 0.4437694235181695 1104 98-99 0.4582392336712502 1104 100 0.5017087982035235 1105 1 0.24285700549709333 1105 2 0.1826556719140413 1105 3 0.1589967119209703 1105 4 0.1503996290567855 1105 5 0.05011962190900476 1105 6 0.07890349574253719 1105 7 0.13493791927898968 1105 8 0.13156817991412595 1105 9 0.09345159926299118 1105 10-11 0.12071344283575058 1105 12-13 0.12786993246017886 1105 14-15 0.23871407476089956 1105 16-17 0.27710839754082883 1105 18-19 0.25590849869441 1105 20-21 0.2394581030156715 1105 22-23 0.23982237896176883 1105 24-25 0.23219257574470475 1105 26-27 0.22877907163638156 1105 28-29 0.231643446022332 1105 30-31 0.26677409220798154 1105 32-33 0.2075211479098158 1105 34-35 0.2219919883854331 1105 36-37 0.220080357303452 1105 38-39 0.1902444905844689 1105 40-41 0.1943485686175208 1105 42-43 0.229217115487792 1105 44-45 0.2566168876717896 1105 46-47 0.32100204902835827 1105 48-49 0.41871939493335475 1105 50-51 0.2816808848780852 1105 52-53 0.2186587864846956 1105 54-55 0.21132887254345434 1105 56-57 0.2236122875162252 1105 58-59 0.21299976043655988 1105 60-61 0.15891236828714028 1105 62-63 0.2018400245127907 1105 64-65 0.2016928727217362 1105 66-67 0.17490920000027899 1105 68-69 0.14619296139265714 1105 70-71 0.13821662626970266 1105 72-73 0.14139743946899586 1105 74-75 0.14990953678466212 1105 76-77 0.1729404539043955 1105 78-79 0.132814767429565 1105 80-81 0.1475842234480922 1105 82-83 0.1532291284839431 1105 84-85 0.06375938409672699 1105 86-87 0.1128523888349875 1105 88-89 0.18032676438042472 1105 90-91 0.1360534062700296 1105 92-93 0.16320107493847757 1105 94-95 0.1652910491936339 1105 96-97 0.1673702252394449 1105 98-99 0.10825281016477817 1105 100 0.1437582403802793 1106 1 0.10298620485268373 1106 2 0.06643689335210468 1106 3 0.10798653604485509 1106 4 0.12921541084685373 1106 5 0.10569640418023596 1106 6 0.1286022833623761 1106 7 0.09208923942657066 1106 8 0.1365681283886957 1106 9 0.11893977629063102 1106 10-11 0.1265748568679399 1106 12-13 0.14948755880779885 1106 14-15 0.22422697342570785 1106 16-17 0.3048595318269349 1106 18-19 0.15324969401569177 1106 20-21 0.18254839750067475 1106 22-23 0.18001800120267575 1106 24-25 0.18158614785480864 1106 26-27 0.19802638286768115 1106 28-29 0.22050556370694352 1106 30-31 0.24712381247774573 1106 32-33 0.20969908820849525 1106 34-35 0.2171766066440881 1106 36-37 0.18551075714871956 1106 38-39 0.23609313952163546 1106 40-41 0.23330615367609653 1106 42-43 0.2746449734118457 1106 44-45 0.2907586285813082 1106 46-47 0.2634237680276925 1106 48-49 0.3850207727233581 1106 50-51 0.2861691461960376 1106 52-53 0.1742508678452026 1106 54-55 0.15713039552947095 1106 56-57 0.17721350552455561 1106 58-59 0.14380229675604994 1106 60-61 0.19569729770654476 1106 62-63 0.19058317130028257 1106 64-65 0.1848925438045299 1106 66-67 0.14824967819325963 1106 68-69 0.19545180110867477 1106 70-71 0.14371346485426528 1106 72-73 0.14953700415573223 1106 74-75 0.09517301537478318 1106 76-77 0.10162952277117654 1106 78-79 0.14865639305425304 1106 80-81 0.15732292610118037 1106 82-83 0.1408589956883759 1106 84-85 0.19406144994939467 1106 86-87 0.1710228819914832 1106 88-89 0.2562462488936035 1106 90-91 0.22799439300580104 1106 92-93 0.19406064594567596 1106 94-95 0.30995714987326295 1106 96-97 0.17177802761643335 1106 98-99 0.13824530066361973 1106 100 0.14281426807612263 1107 1 0.05982828992465272 1107 2 0.049360576066419526 1107 3 0.07628546049680551 1107 4 0.05185149708743353 1107 5 0.047772327996732145 1107 6 0.06881832746240235 1107 7 0.08746523859241506 1107 8 0.056999803446096564 1107 9 0.09911532257343225 1107 10-11 0.0893470145940114 1107 12-13 0.09651406289299302 1107 14-15 0.20924292860169658 1107 16-17 0.2726228463327658 1107 18-19 0.1864581254704092 1107 20-21 0.11764675988187179 1107 22-23 0.1784136305468209 1107 24-25 0.17395714897816816 1107 26-27 0.18988280005520863 1107 28-29 0.15445155317377157 1107 30-31 0.23218550844803332 1107 32-33 0.19924264426506966 1107 34-35 0.16251918551991196 1107 36-37 0.10104915088940913 1107 38-39 0.11837109009797331 1107 40-41 0.13022435536446864 1107 42-43 0.1240148816744977 1107 44-45 0.167911765809599 1107 46-47 0.15119995881303794 1107 48-49 0.3024724104405294 1107 50-51 0.2834923260567166 1107 52-53 0.1836835541184385 1107 54-55 0.20901840829360907 1107 56-57 0.1710238661587482 1107 58-59 0.13741002075314412 1107 60-61 0.15006778141790278 1107 62-63 0.13035471816541389 1107 64-65 0.16530466344277528 1107 66-67 0.11724934669545917 1107 68-69 0.08061523345853061 1107 70-71 0.14606813208753522 1107 72-73 0.1073070617399523 1107 74-75 0.1203998011839218 1107 76-77 0.0784504982206613 1107 78-79 0.1434473527059481 1107 80-81 0.08372316001861435 1107 82-83 0.13985958392482445 1107 84-85 0.11061142840696192 1107 86-87 0.08967509259981199 1107 88-89 0.20039993599508676 1107 90-91 0.12511166551666264 1107 92-93 0.06753263290610079 1107 94-95 0.08702795367181437 1107 96-97 0.07883888156263197 1107 98-99 0.1572312961111848 1107 100 0.11440751339039679 1108 1 0.10236179254036415 1108 2 0.07681327057252929 1108 3 0.06080602659530143 1108 4 0.059828762711617856 1108 5 0.08135830244858511 1108 6 0.13832728983364717 1108 7 0.058266760233863124 1108 8 0.11992116883858728 1108 9 0.10509886551481884 1108 10-11 0.1225581121840591 1108 12-13 0.09752398614595137 1108 14-15 0.1763139683013577 1108 16-17 0.23033324522930343 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-0.3178076532403935 2214 84-85 -0.31450914395210106 2214 86-87 -0.33682938124493234 2214 88-89 -0.1862705875052484 2214 90-91 -0.2552781772216619 2214 92-93 -0.22645806418056935 2214 94-95 -0.21225210061198396 2214 96-97 -0.19435635078174585 2214 98-99 -0.19104330591873975 2214 100 -0.3583521066723847 2215 1 -0.3367820465903364 2215 2 -0.3203756859144562 2215 3 -0.345521327264791 2215 4 -0.2788198235903252 2215 5 -0.2370768041040563 2215 6 -0.23867913865291968 2215 7 -0.22139890266616646 2215 8 -0.2644788941581595 2215 9 -0.36517522748446396 2215 10-11 -0.34734471955665924 2215 12-13 -0.3627444215991815 2215 14-15 -0.3577360080535641 2215 16-17 -0.32945735199144366 2215 18-19 -0.3861194688903993 2215 20-21 -0.42092348642318456 2215 22-23 -0.4468370652748277 2215 24-25 -0.40768959140964967 2215 26-27 -0.4591832994353311 2215 28-29 -0.47147595842138657 2215 30-31 -0.4049875588826666 2215 32-33 -0.48467402107276314 2215 34-35 -0.5173003043964428 2215 36-37 -0.5843098634315496 2215 38-39 -0.5188315963373284 2215 40-41 -0.524410485486861 2215 42-43 -0.5553622974453489 2215 44-45 -0.5400602459199888 2215 46-47 -0.5081106790824705 2215 48-49 -0.42087382241500393 2215 50-51 -0.4555049284236361 2215 52-53 -0.4328893035635488 2215 54-55 -0.38367529645419296 2215 56-57 -0.48715461905519675 2215 58-59 -0.4842991288715268 2215 60-61 -0.4580218255005519 2215 62-63 -0.3883679654667347 2215 64-65 -0.37022847064875464 2215 66-67 -0.4729209467204072 2215 68-69 -0.38512688002649753 2215 70-71 -0.34577514417290445 2215 72-73 -0.3277271040895471 2215 74-75 -0.27697115533621286 2215 76-77 -0.32865423325471 2215 78-79 -0.3201876604747298 2215 80-81 -0.36326894122711195 2215 82-83 -0.4084492106622406 2215 84-85 -0.3470236528941584 2215 86-87 -0.31445006885403615 2215 88-89 -0.30937852988775916 2215 90-91 -0.37415168602035465 2215 92-93 -0.33557713878270334 2215 94-95 -0.31895564049147396 2215 96-97 -0.23522759140138305 2215 98-99 -0.3098375924009744 2215 100 -0.45043486121833354 2216 1 -0.6015583969443412 2216 2 -0.5813509145981577 2216 3 -0.5663431263452949 2216 4 -0.38954211961589635 2216 5 -0.3482317794886072 2216 6 -0.38040991903139343 2216 7 -0.3997392094827319 2216 8 -0.3959146062319405 2216 9 -0.5025789773925453 2216 10-11 -0.5608132199003535 2216 12-13 -0.5175352592330569 2216 14-15 -0.6575084402330447 2216 16-17 -0.6296343762572576 2216 18-19 -0.648079494880939 2216 20-21 -0.594424491524542 2216 22-23 -0.577129557672059 2216 24-25 -0.6014816713469813 2216 26-27 -0.6475352638236487 2216 28-29 -0.6728125335899833 2216 30-31 -0.6707715655226352 2216 32-33 -0.7707321994249838 2216 34-35 -0.7595660085743248 2216 36-37 -0.7993508957220712 2216 38-39 -0.7368575612273602 2216 40-41 -0.7286260061008747 2216 42-43 -0.6973830535337413 2216 44-45 -0.6152405518282862 2216 46-47 -0.6886242639994791 2216 48-49 -0.627447658728002 2216 50-51 -0.7282247317206796 2216 52-53 -0.6637228448160144 2216 54-55 -0.6406893567736773 2216 56-57 -0.6906555047010485 2216 58-59 -0.7438938681050544 2216 60-61 -0.8014500009807648 2216 62-63 -0.8463659026426811 2216 64-65 -0.7695671202823959 2216 66-67 -0.7915612750152832 2216 68-69 -0.6600892828432592 2216 70-71 -0.7268993328610307 2216 72-73 -0.6651461260291214 2216 74-75 -0.6537261657249474 2216 76-77 -0.7109048400159352 2216 78-79 -0.6032530891672181 2216 80-81 -0.6642346256525187 2216 82-83 -0.6527396518764093 2216 84-85 -0.6706621282750476 2216 86-87 -0.5665984829501767 2216 88-89 -0.5451049924741085 2216 90-91 -0.6092938487356108 2216 92-93 -0.5632931788928204 2216 94-95 -0.5136727392210432 2216 96-97 -0.5967400788505159 2216 98-99 -0.6451173116879687 2216 100 -0.7010817229512227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 30.0 8 135.0 9 258.0 10 320.0 11 209.0 12 209.0 13 246.0 14 447.0 15 822.0 16 1676.0 17 3098.0 18 5147.0 19 7594.0 20 10965.0 21 16319.0 22 22938.0 23 25189.0 24 22791.0 25 19087.0 26 18006.0 27 20335.0 28 25441.0 29 31947.0 30 38679.0 31 49749.0 32 63321.0 33 82813.0 34 118597.0 35 181664.0 36 315281.0 37 713723.0 38 1058108.0 39 373944.0 40 841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.179829341391525 20.193662843979887 13.854711069278974 23.771796745349615 2 15.270545661256435 21.36351223571942 40.10439017080283 23.261551932221316 3 17.79392815058692 26.646340066416464 31.442028631031953 24.117703151964662 4 12.036261775923759 18.381514994100616 39.65498281914678 29.927240410828848 5 12.280636573074288 38.65285676734295 35.77096447304014 13.295542186542624 6 28.88576310828229 39.48450215201016 18.253585161300908 13.376149578406643 7 25.16112593256752 33.9950717152431 23.374181521749563 17.469620830439823 8 25.082649157037423 35.2426284506294 20.947931185869116 18.726791206464068 9 26.072067241127357 15.175694318882648 21.188428968076067 37.56380947191392 10-11 23.910086415359373 26.56968500956756 29.30639069315419 20.213837881918874 12-13 25.328795977991465 24.654508605995456 28.83388904738913 21.18280636862395 14-15 22.44464544648589 25.714702353318348 27.42062246888747 24.420029731308293 16-17 21.1207276218494 28.439269152064007 28.10757357884616 22.332429647240435 18-19 20.881255620292173 27.479318021421857 30.62956096046178 21.00986539782419 20-21 22.106945942803875 26.39291697956127 30.375645352371567 21.12449172526329 22-23 21.862167868086267 26.390920667296403 30.11199317384376 21.634918290773577 24-25 21.7087744034002 26.53032001632234 30.453811833015525 21.307093747261938 26-27 21.631062069648785 26.617764526675586 30.25108981027394 21.500083593401694 28-29 21.362333773384425 27.11207494408544 30.14570293826983 21.379888344260305 30-31 21.81837855811666 26.56999558658005 30.052495083858847 21.559130771444444 32-33 21.765064568906705 26.798128065960736 29.76162114156644 21.675186223566115 34-35 22.14900235980931 26.710660505114365 29.997922550451683 21.142414584624643 36-37 22.059105360585548 26.381973957925364 29.984795321999535 21.57412535948955 38-39 21.83458084203122 26.604505892435192 30.042750496609187 21.5181627689244 40-41 22.07122393291581 26.436622179895643 29.94617654384648 21.54597734334207 42-43 21.94415109434294 26.47335281982979 29.543404824068887 22.039091261758387 44-45 21.994271360066676 26.758535024876068 29.38043348865751 21.866760126399747 46-47 22.333107012569005 26.723064191194297 28.770756261205744 22.173072535030954 48-49 22.55595217657632 26.72214992088822 28.60467128710634 22.11722661542913 50-51 22.238460969265887 26.927867454671606 28.440857368691386 22.392814207371124 52-53 22.200085420191336 27.174967890026846 28.187625961112605 22.43732072866922 54-55 22.10008964633584 27.0772028175978 28.19187628650851 22.630831249557843 56-57 22.426190122355973 26.78767585574875 28.19484693119334 22.591287090701936 58-59 22.187537588278758 26.88609371848293 28.423403040933355 22.502965652304958 60-61 22.217729488805716 27.13356762284487 28.244079473495674 22.40462341485374 62-63 22.04962232775641 26.895892371228115 28.2454936148514 22.80899168616407 64-65 21.65566818832623 26.978731493523128 28.296907985082587 23.068692333068054 66-67 22.045989324680793 26.786567922028066 28.050484847734282 23.116957905556863 68-69 21.85419596376541 26.749358018132156 28.280284298736596 23.116161719365838 70-71 21.922091094818104 26.582271622437293 28.175120490815175 23.320516791929432 72-73 22.077412219729485 26.496458323141862 28.359561784442878 23.06656767268577 74-75 21.963443953019272 26.948760540399125 28.054606504666644 23.03318900191496 76-77 21.873628974754094 26.727905923324453 27.939795485218706 23.45866961670275 78-79 21.846843987847237 26.750287858724292 28.00205146749898 23.40081668592949 80-81 21.904844761765144 26.789778167402268 27.96652084644975 23.33885622438284 82-83 22.000602494127538 26.479725267631544 28.28473079875055 23.234941439490363 84-85 22.070449706446656 26.606261418672428 27.873573449638023 23.449715425242896 86-87 21.64265839899267 26.850094847286115 27.901000300625807 23.60624645309541 88-89 21.858762220469863 26.48694444986252 27.728008881928982 23.926284447738634 90-91 21.76865525041808 26.39289074046066 27.931593532178805 23.906860476942445 92-93 21.964163254564735 26.462162434112248 27.8114197698089 23.76225454151412 94-95 21.447933877133117 26.33250323188469 28.03252643674709 24.187036454235102 96-97 21.443189234642634 26.21805025270007 28.089987479550555 24.248773033106744 98-99 21.367557223429507 26.462594975056714 27.863214865227604 24.306632936286178 100 21.597583268783886 26.136514329976762 27.569233152594887 24.69666924864446 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 105.0 1 77.5 2 188.0 3 328.0 4 224.5 5 165.5 6 346.0 7 997.0 8 1972.0 9 2416.0 10 1788.5 11 1193.0 12 1300.0 13 1510.5 14 1788.0 15 2080.0 16 2486.5 17 2901.5 18 3293.0 19 3813.5 20 4432.5 21 5257.0 22 6262.0 23 7582.5 24 9349.5 25 11817.5 26 15619.0 27 20393.0 28 25465.5 29 32076.5 30 39503.5 31 46758.0 32 55993.5 33 63698.5 34 70794.0 35 78037.5 36 85113.0 37 95086.5 38 99924.0 39 100504.5 40 102617.5 41 103341.0 42 104382.0 43 108719.5 44 115284.0 45 123445.5 46 129584.5 47 136082.5 48 144160.5 49 141323.0 50 130499.5 51 123292.5 52 117709.0 53 111808.5 54 105801.0 55 95046.0 56 84038.5 57 73294.0 58 61283.0 59 52309.0 60 42186.0 61 31801.0 62 24519.5 63 18747.0 64 13198.0 65 8838.5 66 6269.5 67 4570.0 68 3338.5 69 2433.5 70 1767.0 71 1320.0 72 974.0 73 863.0 74 698.0 75 525.5 76 410.0 77 270.5 78 181.0 79 124.5 80 92.0 81 70.5 82 47.5 83 25.0 84 14.5 85 11.5 86 9.5 87 5.5 88 2.0 89 1.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002755478525998559 2 0.003808133243795761 3 2.1672303013471814E-4 4 0.05034166385700738 5 0.002229151167099958 6 0.004891748394469353 7 6.192086575277661E-5 8 3.405647616402713E-4 9 3.096043287638831E-5 10-11 1.7028238082013566E-4 12-13 1.5480216438194154E-5 14-15 1.5480216438194154E-5 16-17 2.941241123256889E-4 18-19 1.0836151506735907E-4 20-21 2.322032465729123E-4 22-23 0.0 24-25 0.0 26-27 2.7864389588749475E-4 28-29 3.096043287638831E-5 30-31 2.322032465729123E-4 32-33 2.322032465729123E-4 34-35 2.1672303013471814E-4 36-37 6.192086575277661E-5 38-39 7.740108219097077E-4 40-41 5.418075753367953E-4 42-43 0.0 44-45 5.418075753367953E-4 46-47 0.0 48-49 1.7028238082013566E-4 50-51 0.0 52-53 4.1796584383124215E-4 54-55 4.489262767076304E-4 56-57 2.7864389588749475E-4 58-59 0.00100621406848262 60-61 6.811295232805426E-4 62-63 3.7152519451665966E-4 64-65 3.8700541095485386E-4 66-67 0.001517061210943027 68-69 0.0026935576602457826 70-71 0.003668811295852014 72-73 0.003188924586267995 74-75 0.0029102806903805005 76-77 0.0042725397369415856 78-79 0.0014241799123138619 80-81 0.0011919766657409498 82-83 6.811295232805426E-4 84-85 6.811295232805426E-4 86-87 0.0 88-89 0.0 90-91 1.5480216438194154E-5 92-93 1.2384173150555323E-4 94-95 7.740108219097077E-5 96-97 4.0248562739304795E-4 98-99 6.192086575277661E-5 100 0.07520289145674719 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3229929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.663372191136354 #Duplication Level Percentage of deduplicated Percentage of total 1 68.58699325407595 38.17783332972587 2 16.439053681372496 18.30106327072615 3 6.580275676910925 10.988410023725235 4 3.2330456427907106 7.19848891702364 5 1.7811634187222116 4.957278115478566 6 1.0552380143695108 3.524286380645146 7 0.6693774855820586 2.6081865681424796 8 0.4304499758966049 1.9168237774398709 9 0.2905107774785227 1.455372857909106 >10 0.9120511029187999 7.422504329312965 >50 0.011821693431495441 0.4608579899135505 >100 0.008379248022645449 0.9214272106715407 >500 8.631728568887827E-4 0.32777739805229456 >1k 5.179037141331586E-4 0.542547988684538 >5k 1.7263457137771954E-4 0.7033136254307711 >10k+ 8.631728568885977E-5 0.49382821711828573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10278 0.31821132910351896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9455 0.2927308928462514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5183 0.16046792359832057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4225 0.1308078289027406 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.096043287638831E-5 2 0.0 0.0 0.0 0.0 3.096043287638831E-5 3 0.0 0.0 0.0 0.0 3.096043287638831E-5 4 0.0 0.0 0.0 0.0 3.096043287638831E-5 5 0.0 0.0 0.0 0.0 3.096043287638831E-5 6 0.0 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 7 0.0 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 8 0.0 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 9 0.0 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 10-11 0.0 3.096043287638831E-5 0.0 0.0 3.096043287638831E-5 12-13 0.0 3.096043287638831E-5 0.0 3.096043287638831E-5 1.0836151506735906E-4 14-15 0.0 3.096043287638831E-5 0.0 6.192086575277661E-5 1.5480216438194152E-4 16-17 0.0 3.096043287638831E-5 0.0 6.192086575277661E-5 1.5480216438194152E-4 18-19 0.0 3.096043287638831E-5 0.0 1.0836151506735907E-4 1.702823808201357E-4 20-21 0.0 3.096043287638831E-5 0.0 1.5480216438194154E-4 2.1672303013471814E-4 22-23 0.0 3.096043287638831E-5 0.0 5.10847142460407E-4 2.1672303013471814E-4 24-25 0.0 3.096043287638831E-5 0.0 0.0011919766657409498 2.1672303013471814E-4 26-27 0.0 3.096043287638831E-5 0.0 0.0023684731150437053 2.1672303013471814E-4 28-29 0.0 3.096043287638831E-5 0.0 0.00775558843553527 2.1672303013471814E-4 30-31 0.0 3.096043287638831E-5 0.0 0.0219664271257975 2.1672303013471814E-4 32-33 0.0 3.096043287638831E-5 0.0 0.05175036355288305 2.4768346301110646E-4 34-35 0.0 3.096043287638831E-5 0.0 0.09884118195786967 3.0960432876388303E-4 36-37 0.0 3.096043287638831E-5 0.0 0.1731152604283252 3.0960432876388303E-4 38-39 0.0 3.096043287638831E-5 0.0 0.2803312394792579 3.0960432876388303E-4 40-41 0.0 3.096043287638831E-5 0.0 0.404482575313575 3.0960432876388303E-4 42-43 0.0 3.096043287638831E-5 0.0 0.5264047599807922 3.0960432876388303E-4 44-45 0.0 3.096043287638831E-5 0.0 0.6796124620695996 3.0960432876388303E-4 46-47 0.0 3.096043287638831E-5 0.0 0.8653750593279295 3.0960432876388303E-4 48-49 0.0 3.096043287638831E-5 0.0 1.0649924502984431 3.0960432876388303E-4 50-51 0.0 3.096043287638831E-5 0.0 1.2816071189180938 3.405647616402714E-4 52-53 0.0 3.096043287638831E-5 0.0 1.4902339958556365 3.7152519451665966E-4 54-55 0.0 3.096043287638831E-5 0.0 1.7198830067162467 3.7152519451665966E-4 56-57 0.0 3.096043287638831E-5 0.0 2.002087352384526 3.870054109548538E-4 58-59 0.0 3.096043287638831E-5 0.0 2.3197568739127084 4.0248562739304795E-4 60-61 0.0 3.096043287638831E-5 0.0 2.6504297772489736 4.0248562739304795E-4 62-63 0.0 3.096043287638831E-5 0.0 2.9894310370289876 4.953669260222129E-4 64-65 0.0 3.096043287638831E-5 0.0 3.3401663008691522 4.953669260222129E-4 66-67 0.0 3.096043287638831E-5 0.0 3.7404382573115385 5.10847142460407E-4 68-69 0.0 3.096043287638831E-5 0.0 4.1911757193424375 5.263273588986012E-4 70-71 0.0 3.096043287638831E-5 0.0 4.675025364334633 5.572877917749895E-4 72-73 0.0 3.096043287638831E-5 0.0 5.174835112474609 6.03728441089572E-4 74-75 0.0 3.096043287638831E-5 0.0 5.671208252565304 6.192086575277661E-4 76-77 0.0 3.096043287638831E-5 0.0 6.217489610452738 6.192086575277661E-4 78-79 0.0 3.096043287638831E-5 0.0 6.826280082317599 6.192086575277661E-4 80-81 0.0 3.096043287638831E-5 0.0 7.477207703327225 6.192086575277661E-4 82-83 0.0 3.096043287638831E-5 0.0 8.152083219166737 6.811295232805427E-4 84-85 0.0 3.096043287638831E-5 0.0 8.810828349477651 6.811295232805427E-4 86-87 0.0 3.096043287638831E-5 0.0 9.496988943100607 7.275701725951251E-4 88 0.0 3.096043287638831E-5 0.0 10.038517874541515 7.740108219097076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11500 0.0 35.328568 1 GTATCAA 17400 0.0 30.754108 1 ATCAACG 19965 0.0 26.165842 3 CAACGCA 20150 0.0 25.96102 5 TATCAAC 20780 0.0 25.502443 2 AACGCAG 21125 0.0 25.052053 6 TCAACGC 20955 0.0 24.996975 4 GAGTACT 13645 0.0 23.094402 12-13 ACGCAGA 22875 0.0 22.908426 7 CGCAGAG 23100 0.0 22.603909 8 GTACTAG 1095 0.0 21.901114 1 AGAGTAC 18365 0.0 21.714128 10-11 GTACTTT 14735 0.0 21.162758 14-15 GTACATG 10180 0.0 21.063337 1 GCAGAGT 24745 0.0 20.740057 9 ACATGGG 9955 0.0 20.499271 3 TAGTACT 1635 0.0 20.418816 4 CTGTGCG 1365 0.0 20.313938 9 TACATGG 10265 0.0 20.247282 2 AGTACTT 14505 0.0 19.95926 12-13 >>END_MODULE