FastQCFastQC Report
Mon 16 Jan 2017
ERR1146516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502798
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57600.033.3119621
GGTATCA38050.029.7868751
CAACGCA60200.028.7374825
TATCAAC68200.028.1254372
ATCAACG62550.027.9595643
AACGCAG66200.027.046316
GTACTAG12350.026.6559181
GTACATG94350.026.2682571
TACATGG92000.025.7041722
ACGCAGA68650.024.9168517
GCTATTG21500.024.916678
CGCAGAG68700.024.7614158
ACATGGG94050.024.2436353
TCAACGC75800.023.1332724
CTATTGA23700.022.7992749
TATTCGC2704.7293724E-1122.6230499
TAGGCAT19500.022.4205255
TCGCCGG2103.7918653E-822.4183894
TTTAGGG24550.022.4056642
ATTTAGG24400.022.3578851
Produced by FastQC (version 0.11.5)