FastQCFastQC Report
Mon 16 Jan 2017
ERR1146516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502798
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96720.38644748797146233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96220.38444972386904575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.20736791383084052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.054.9732631
GTATCAA122450.041.667631
CAACGCA149900.033.362575
ATCAACG151400.033.048543
TATCAAC155000.032.7657362
AACGCAG161550.031.5676546
TCAACGC166850.030.2419684
ACGCAGA172750.029.0017367
CGCAGAG173800.028.74548
GCAGAGT186450.026.4669029
GTACTAG12350.025.1307141
GTGTACG1351.4523634E-524.3832781
GAGTACT128550.023.80068412-13
TACATGG91400.022.9979522
CAGAGTA183100.022.95957410-11
GTACATG95000.022.571951
GTGGTAT20800.022.382041
AGAGTAC177450.022.08827810-11
CTAGTAC18150.022.0231193
TAGTACT17800.021.9277784
Produced by FastQC (version 0.11.5)