FastQCFastQC Report
Mon 16 Jan 2017
ERR1146515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5609947
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59030.10522381049232728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.033.3087271
GTATCAA109700.031.8948251
CAACGCA118650.028.0914065
ATCAACG119550.027.9598183
TATCAAC134400.026.0947932
AACGCAG129850.025.9863766
TCAACGC135300.024.9133344
GTACATG186350.024.5296171
GGCGTCG13300.024.0275148
ACGCAGA138950.023.7431247
TACATGG181450.023.70712
CGCAGAG140800.023.1303468
ACATGGG178600.023.0324943
TACGGGC15350.021.4387824
ACGGGCG15300.020.5863025
CATGGGG128900.020.5701124
CGGGCGT15300.020.2728926
ATACGGG15650.020.1268023
GTACTAG25600.020.023521
GCAGAGT159450.020.0115789
Produced by FastQC (version 0.11.5)