##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146515_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5609947 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.234673340051163 31.0 31.0 34.0 28.0 34.0 2 31.259620278052537 31.0 31.0 34.0 28.0 34.0 3 31.45158412370028 33.0 31.0 34.0 28.0 34.0 4 34.88364471179496 37.0 35.0 37.0 32.0 37.0 5 34.93213643551356 37.0 35.0 37.0 32.0 37.0 6 34.94238091732417 37.0 35.0 37.0 32.0 37.0 7 34.90792533334094 37.0 35.0 37.0 32.0 37.0 8 34.844956110993564 37.0 35.0 37.0 32.0 37.0 9 36.413217807583564 39.0 37.0 39.0 32.0 39.0 10-11 36.42346781529308 39.0 37.0 39.0 32.0 39.0 12-13 36.37019244566838 39.0 37.0 39.0 32.0 39.0 14-15 37.71505702282036 40.0 38.0 41.0 32.0 41.0 16-17 37.7551374371273 40.0 38.0 41.0 32.0 41.0 18-19 37.79332113119786 40.0 38.0 41.0 32.0 41.0 20-21 37.91006225192502 40.0 38.0 41.0 32.0 41.0 22-23 37.941930734817994 40.0 38.0 41.0 32.0 41.0 24-25 37.98585557047152 40.0 38.0 41.0 32.0 41.0 26-27 38.06285398061693 40.0 38.0 41.0 32.0 41.0 28-29 38.05570658688932 40.0 38.0 41.0 32.0 41.0 30-31 38.02236643233884 40.0 38.0 41.0 32.0 41.0 32-33 37.99329146959855 40.0 38.0 41.0 31.0 41.0 34-35 37.94554636612431 40.0 38.0 41.0 31.0 41.0 36-37 37.87213462087966 40.0 38.0 41.0 31.0 41.0 38-39 37.81140909174365 40.0 38.0 41.0 31.0 41.0 40-41 37.76554626986672 40.0 38.0 41.0 31.0 41.0 42-43 37.6125126494065 40.0 37.5 41.0 30.0 41.0 44-45 37.50131355964682 40.0 37.0 41.0 30.0 41.0 46-47 37.40572745161407 40.0 37.0 41.0 30.0 41.0 48-49 37.30628212708605 40.0 37.0 41.0 30.0 41.0 50-51 37.18772574856768 40.0 36.0 41.0 30.0 41.0 52-53 37.07467414576287 40.0 36.0 41.0 30.0 41.0 54-55 36.97089339703209 40.0 35.5 41.0 30.0 41.0 56-57 36.802078611437864 39.0 35.0 41.0 30.0 41.0 58-59 36.632186988575825 39.0 35.0 41.0 30.0 41.0 60-61 36.43024925190915 39.0 35.0 41.0 29.5 41.0 62-63 36.21379488968434 38.5 35.0 40.5 29.0 41.0 64-65 35.93160621660062 38.0 35.0 40.0 29.0 41.0 66-67 35.69970981900542 37.0 35.0 40.0 29.0 41.0 68-69 35.447758953872466 37.0 34.5 40.0 29.0 41.0 70-71 35.167224574492415 36.0 34.0 39.0 28.5 41.0 72-73 34.824316432936 36.0 34.0 39.0 28.0 41.0 74-75 34.48482178173876 35.0 34.0 39.0 28.0 40.5 76-77 34.20016249707885 35.0 34.0 37.5 28.0 39.5 78-79 33.87461530385225 35.0 34.0 37.0 27.0 39.0 80-81 33.6178914881014 35.0 34.0 36.5 27.0 39.0 82-83 33.33472651345904 35.0 33.5 36.0 27.0 38.0 84-85 33.116768304584696 35.0 33.0 36.0 27.0 37.0 86-87 32.90212581330982 35.0 33.0 35.5 26.5 37.0 88-89 32.71653662681662 35.0 33.0 35.0 26.0 36.5 90-91 32.56371673386576 35.0 33.0 35.0 26.0 36.0 92-93 32.41201235947506 35.0 33.0 35.0 26.0 36.0 94-95 32.288797380795216 35.0 33.0 35.0 25.0 36.0 96-97 32.19906640829227 35.0 33.0 35.0 25.0 35.5 98-99 32.09453698938689 35.0 33.0 35.0 25.0 35.0 100 30.98237113470056 34.0 31.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38867736437945055 1101 2 0.333656153713374 1101 3 0.35780321077929145 1101 4 0.28775551605524186 1101 5 0.24144168517566555 1101 6 0.2818947084568535 1101 7 0.3178014684730499 1101 8 0.3256575633186216 1101 9 0.46684334678986517 1101 10-11 0.4481771495164537 1101 12-13 0.4010044350412372 1101 14-15 0.5250416254305534 1101 16-17 0.4809464445217557 1101 18-19 0.457294483990367 1101 20-21 0.48198370065296103 1101 22-23 0.5444799337686064 1101 24-25 0.5696070987412654 1101 26-27 0.5518999434906888 1101 28-29 0.5688120153792084 1101 30-31 0.5930832291570951 1101 32-33 0.5849434067968957 1101 34-35 0.5242963688361328 1101 36-37 0.5701105383955962 1101 38-39 0.5988056887961264 1101 40-41 0.5693126533644275 1101 42-43 0.5778918136092628 1101 44-45 0.5087633169016357 1101 46-47 0.5041707301268374 1101 48-49 0.511441166791883 1101 50-51 0.5157872833869774 1101 52-53 0.4746140806431782 1101 54-55 0.4662757032163327 1101 56-57 0.4887770671027525 1101 58-59 0.5294065524992888 1101 60-61 0.48664675459771445 1101 62-63 0.48187542861439425 1101 64-65 0.526128324112328 1101 66-67 0.49690190239842735 1101 68-69 0.4169794186875464 1101 70-71 0.41594870564568964 1101 72-73 0.4748061803848671 1101 74-75 0.44226402035182844 1101 76-77 0.45178662714478435 1101 78-79 0.4378712228602737 1101 80-81 0.4302097116223109 1101 82-83 0.43325000516832546 1101 84-85 0.34578483948315863 1101 86-87 0.3950230230973304 1101 88-89 0.3502219292492157 1101 90-91 0.33361046439487296 1101 92-93 0.41394961239942063 1101 94-95 0.3591735221729664 1101 96-97 0.35025074344364526 1101 98-99 0.32437771749953725 1101 100 0.2713401044025652 1102 1 0.4156966045989918 1102 2 0.42840817230162287 1102 3 0.4468615023902025 1102 4 0.44672158754994484 1102 5 0.411910236349911 1102 6 0.492492388397082 1102 7 0.49259080684868195 1102 8 0.468424330146334 1102 9 0.6215920001749637 1102 10-11 0.5999704194999396 1102 12-13 0.5637292695444174 1102 14-15 0.6764646673830228 1102 16-17 0.6334367830198033 1102 18-19 0.6343026847472828 1102 20-21 0.6244885180628543 1102 22-23 0.5965044060159315 1102 24-25 0.6679159031837045 1102 26-27 0.6217311199911322 1102 28-29 0.6036862578488709 1102 30-31 0.6564383663958537 1102 32-33 0.6506780006676465 1102 34-35 0.6307326029232243 1102 36-37 0.6673280091270968 1102 38-39 0.6606106794499382 1102 40-41 0.6428578132120393 1102 42-43 0.6865100190613234 1102 44-45 0.6125105160846331 1102 46-47 0.6061225108306658 1102 48-49 0.6757537535147833 1102 50-51 0.6293937296535645 1102 52-53 0.5944641852512476 1102 54-55 0.5631320317796948 1102 56-57 0.6271134316866025 1102 58-59 0.5846827891812794 1102 60-61 0.6409015045596931 1102 62-63 0.5975614423530757 1102 64-65 0.6354724983619136 1102 66-67 0.5944549560882635 1102 68-69 0.5894342842692097 1102 70-71 0.5304897521681937 1102 72-73 0.5835443953292838 1102 74-75 0.5804651642796017 1102 76-77 0.526057560894003 1102 78-79 0.4873134910183339 1102 80-81 0.4737826515653154 1102 82-83 0.5462550675438465 1102 84-85 0.5186248658368058 1102 86-87 0.5188338678681959 1102 88-89 0.48973470426014387 1102 90-91 0.4564606763813188 1102 92-93 0.4492949461870168 1102 94-95 0.484012851356006 1102 96-97 0.4900988601131786 1102 98-99 0.5078022636773909 1102 100 0.5883362828645033 1103 1 0.2520626167369109 1103 2 0.24850458298183398 1103 3 0.2769583213516249 1103 4 0.25473320878182903 1103 5 0.26979534320167886 1103 6 0.25423152908479807 1103 7 0.2779873974893974 1103 8 0.23660822493085476 1103 9 0.36402632057505 1103 10-11 0.29434587947623925 1103 12-13 0.33961772993357187 1103 14-15 0.38641677705298605 1103 16-17 0.3617845041595231 1103 18-19 0.3536421737411075 1103 20-21 0.356280170401547 1103 22-23 0.3805043141526667 1103 24-25 0.43447465396990737 1103 26-27 0.40507184755529124 1103 28-29 0.3928850552495291 1103 30-31 0.46680855954453904 1103 32-33 0.49689215155359534 1103 34-35 0.4294460595551328 1103 36-37 0.3948528412478467 1103 38-39 0.4387447656866712 1103 40-41 0.39775483835547476 1103 42-43 0.4378822398893689 1103 44-45 0.3903113779803462 1103 46-47 0.3751253174120208 1103 48-49 0.3736819287547064 1103 50-51 0.38576284613653655 1103 52-53 0.3453381534172806 1103 54-55 0.30584635917687564 1103 56-57 0.3840980584911833 1103 58-59 0.35263010084309343 1103 60-61 0.37298873841577773 1103 62-63 0.37370023015912324 1103 64-65 0.3354422644343842 1103 66-67 0.36996933761789563 1103 68-69 0.3362139310285315 1103 70-71 0.35294139988204165 1103 72-73 0.3041623412594703 1103 74-75 0.37831244880479176 1103 76-77 0.34501037062545237 1103 78-79 0.3343571830912353 1103 80-81 0.29019015352136535 1103 82-83 0.26891281807628076 1103 84-85 0.19550698062084138 1103 86-87 0.26005014639200397 1103 88-89 0.19641413206106506 1103 90-91 0.1778032334676567 1103 92-93 0.21295034241513378 1103 94-95 0.27074651016455675 1103 96-97 0.23124014336214316 1103 98-99 0.20024825176896144 1103 100 0.2316265507427353 1104 1 0.13411269607527032 1104 2 0.1452334118206835 1104 3 0.10451750228784817 1104 4 0.08198440930183892 1104 5 0.02270877961203155 1104 6 0.1890306303598308 1104 7 0.1502520281529911 1104 8 0.19810192384551328 1104 9 0.23188339270337366 1104 10-11 0.22725833789108663 1104 12-13 0.25335364293240303 1104 14-15 0.3554109961635845 1104 16-17 0.38731730824490995 1104 18-19 0.3205835857061885 1104 20-21 0.31983596809728 1104 22-23 0.2720225701140251 1104 24-25 0.32288746899365606 1104 26-27 0.3232753077875046 1104 28-29 0.33529651545786265 1104 30-31 0.36417003346651455 1104 32-33 0.30979401479103075 1104 34-35 0.3414240900593768 1104 36-37 0.3288848061018612 1104 38-39 0.29948376676396293 1104 40-41 0.3063848958876534 1104 42-43 0.37915313487868474 1104 44-45 0.2760901499706634 1104 46-47 0.28349395443895986 1104 48-49 0.2695713227528813 1104 50-51 0.2666411959746213 1104 52-53 0.25529454105726046 1104 54-55 0.2107059795192967 1104 56-57 0.28915327793519197 1104 58-59 0.23911327375893876 1104 60-61 0.250734009787152 1104 62-63 0.2731881321783547 1104 64-65 0.3264233078701366 1104 66-67 0.24243431493410128 1104 68-69 0.2491171235802483 1104 70-71 0.25132712402328394 1104 72-73 0.2823038703669809 1104 74-75 0.24631391430597205 1104 76-77 0.2039813497956402 1104 78-79 0.22352685947917195 1104 80-81 0.16580611435379922 1104 82-83 0.20245551388766359 1104 84-85 0.17533043140677051 1104 86-87 0.1333463181567751 1104 88-89 0.12468416380997382 1104 90-91 0.15680796908196726 1104 92-93 0.17220689356076946 1104 94-95 0.18742237803722617 1104 96-97 0.12472222379867048 1104 98-99 0.174326148500775 1104 100 0.1714521555603845 1105 1 0.2463600217315296 1105 2 0.2041289230813561 1105 3 0.20364098845798395 1105 4 0.19071759610044836 1105 5 0.22358663272633095 1105 6 0.2362980915197852 1105 7 0.24810418263115963 1105 8 0.2746622938451537 1105 9 0.30644687366942946 1105 10-11 0.3274844059146176 1105 12-13 0.326587979761797 1105 14-15 0.4185658544338082 1105 16-17 0.31481833423244865 1105 18-19 0.38143699528519903 1105 20-21 0.348170539205789 1105 22-23 0.35075692559774296 1105 24-25 0.39244350098724823 1105 26-27 0.36486512769519663 1105 28-29 0.3706930058311144 1105 30-31 0.39774494554409046 1105 32-33 0.42333243455657055 1105 34-35 0.3685549792836511 1105 36-37 0.3991654866369245 1105 38-39 0.37777647726200314 1105 40-41 0.4294153824656277 1105 42-43 0.4097494146016345 1105 44-45 0.3636638704039683 1105 46-47 0.36298266985154015 1105 48-49 0.37247797654115544 1105 50-51 0.43932845902889994 1105 52-53 0.36930013990394883 1105 54-55 0.3232351607732653 1105 56-57 0.39555090414919647 1105 58-59 0.348707285626908 1105 60-61 0.41638797280950257 1105 62-63 0.37227340888248506 1105 64-65 0.36071022500637895 1105 66-67 0.3348584532400949 1105 68-69 0.34824660464260404 1105 70-71 0.2514649268251361 1105 72-73 0.30238872838529574 1105 74-75 0.3562245553353307 1105 76-77 0.32478805820758794 1105 78-79 0.33893967158426364 1105 80-81 0.26686157412217426 1105 82-83 0.23671873844453017 1105 84-85 0.24868398049458307 1105 86-87 0.19945063948554775 1105 88-89 0.20246415158422337 1105 90-91 0.2052259871769735 1105 92-93 0.24430679269989497 1105 94-95 0.23947033526175687 1105 96-97 0.2594013121158696 1105 98-99 0.2587766895238488 1105 100 0.3648608672229692 1106 1 0.06698490705555571 1106 2 0.06764301197473443 1106 3 0.060071222613142794 1106 4 0.09336956916883565 1106 5 0.13164966177309623 1106 6 0.05077194478678848 1106 7 0.08172153298941964 1106 8 0.12901449624257566 1106 9 0.1491071301433422 1106 10-11 0.19076681305821097 1106 12-13 0.20650699274407458 1106 14-15 0.162196317383291 1106 16-17 0.1961537759486376 1106 18-19 0.17439774291219834 1106 20-21 0.21635401984634228 1106 22-23 0.2148359421365953 1106 24-25 0.23788783419525572 1106 26-27 0.17499640332589905 1106 28-29 0.20209877705176638 1106 30-31 0.233159147138835 1106 32-33 0.2715447497592507 1106 34-35 0.22605233973110472 1106 36-37 0.22445935121837124 1106 38-39 0.1880779813066269 1106 40-41 0.15729487037334167 1106 42-43 0.22888657921117073 1106 44-45 0.157026543472071 1106 46-47 0.2340809330099276 1106 48-49 0.18188745619754343 1106 50-51 0.19124594824344143 1106 52-53 0.15935234583044888 1106 54-55 0.09821807682228467 1106 56-57 0.1544094502572264 1106 58-59 0.14374378104056262 1106 60-61 0.18684925247191586 1106 62-63 0.14627746380963202 1106 64-65 0.18973146165984645 1106 66-67 0.156911362843573 1106 68-69 0.12792040773863533 1106 70-71 0.08210681283831889 1106 72-73 0.11680840799685654 1106 74-75 0.07275392911323308 1106 76-77 0.10261300580229715 1106 78-79 0.15648518333662764 1106 80-81 0.08354590655409311 1106 82-83 0.10703725845570489 1106 84-85 0.06893434039189827 1106 86-87 0.06782666118067482 1106 88-89 0.07100762120671789 1106 90-91 0.09147464792201987 1106 92-93 0.12083630855760674 1106 94-95 0.053035244421579364 1106 96-97 0.0769760571704694 1106 98-99 0.12031864194788966 1106 100 0.2187233526082295 1107 1 0.059085231412321804 1107 2 0.08344138640068266 1107 3 0.016042751427207236 1107 4 0.04652461373516559 1107 5 0.05518375557280564 1107 6 0.03465394159909607 1107 7 0.0713795106771471 1107 8 0.11264563527366533 1107 9 0.206858537897034 1107 10-11 0.19426709966909073 1107 12-13 0.15236850361906562 1107 14-15 0.19292815393186658 1107 16-17 0.2307781086063727 1107 18-19 0.19635981415346748 1107 20-21 0.19964921654502632 1107 22-23 0.24924800300360062 1107 24-25 0.24904514429650249 1107 26-27 0.19432759381799514 1107 28-29 0.1595210414222592 1107 30-31 0.21668693432703634 1107 32-33 0.24327274568300794 1107 34-35 0.23773754525669233 1107 36-37 0.20648365238212563 1107 38-39 0.22669659893957572 1107 40-41 0.2176319302341838 1107 42-43 0.20303093731417476 1107 44-45 0.23314312435084616 1107 46-47 0.1946501535416516 1107 48-49 0.21619333232615645 1107 50-51 0.1764891025026074 1107 52-53 0.18647751565764992 1107 54-55 0.1735397862969279 1107 56-57 0.22259958951455872 1107 58-59 0.18441536873977782 1107 60-61 0.17943751865780655 1107 62-63 0.16862412503713387 1107 64-65 0.17962427661628766 1107 66-67 0.11846174208036331 1107 68-69 0.06354510417923365 1107 70-71 0.08065412356084778 1107 72-73 0.12110210072108174 1107 74-75 0.16300851459419619 1107 76-77 0.12034849463697128 1107 78-79 0.12003656366007931 1107 80-81 0.05034310132327846 1107 82-83 0.07669518925412433 1107 84-85 0.060657205844208306 1107 86-87 0.07140402208648311 1107 88-89 0.0980543491342587 1107 90-91 0.0763161171358675 1107 92-93 0.11979771045404419 1107 94-95 0.10631517083662345 1107 96-97 0.07947047050515721 1107 98-99 0.08323379301545941 1107 100 0.21713810335836214 1108 1 0.08571640310182715 1108 2 0.09934889645439782 1108 3 0.04951868214475752 1108 4 0.12758246499348047 1108 5 0.10430630586534306 1108 6 0.13698540530661774 1108 7 0.09971935569923573 1108 8 0.14666213907057823 1108 9 0.08442846982529062 1108 10-11 0.1238389987786519 1108 12-13 0.08074887223788352 1108 14-15 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-0.5004188049037701 2215 3 -0.47965118210590063 2215 4 -0.4831502139974688 2215 5 -0.35315777943144866 2215 6 -0.43216486452278957 2215 7 -0.4735070915039401 2215 8 -0.4792162769294066 2215 9 -0.6535926616769174 2215 10-11 -0.6626329803027033 2215 12-13 -0.595095738063911 2215 14-15 -0.7478892869954166 2215 16-17 -0.7917661679475785 2215 18-19 -0.8151579723089952 2215 20-21 -0.7340438636974511 2215 22-23 -0.6416623745466978 2215 24-25 -0.5957651475785397 2215 26-27 -0.6999889753645263 2215 28-29 -0.7204209956046341 2215 30-31 -0.7468161259186772 2215 32-33 -0.7092412490872846 2215 34-35 -0.7500346861124001 2215 36-37 -0.7271201204510476 2215 38-39 -0.7999947921488868 2215 40-41 -0.7869299004480084 2215 42-43 -0.8350484335016333 2215 44-45 -0.7496801738985255 2215 46-47 -0.6491505597323268 2215 48-49 -0.6303571396505916 2215 50-51 -0.667081629523004 2215 52-53 -0.659611603161629 2215 54-55 -0.6792091949267487 2215 56-57 -0.63824125910768 2215 58-59 -0.5830608384930827 2215 60-61 -0.5710393635442514 2215 62-63 -0.5610839729327424 2215 64-65 -0.5721941824272037 2215 66-67 -0.6148417360559577 2215 68-69 -0.6144151298484317 2215 70-71 -0.4602294353617111 2215 72-73 -0.4344471137345849 2215 74-75 -0.4160457058586218 2215 76-77 -0.4599708808636578 2215 78-79 -0.48251532221373594 2215 80-81 -0.48436968528558566 2215 82-83 -0.46684314096372503 2215 84-85 -0.4430630756043925 2215 86-87 -0.43661391145155193 2215 88-89 -0.38900040692830373 2215 90-91 -0.3914109136527131 2215 92-93 -0.4276597656939245 2215 94-95 -0.45222485604595875 2215 96-97 -0.42082528840802524 2215 98-99 -0.42233059841879594 2215 100 -0.3675115607501347 2216 1 -0.38288379512423987 2216 2 -0.3029007865232458 2216 3 -0.4135071046629335 2216 4 -0.39187656770511126 2216 5 -0.3462914641784849 2216 6 -0.347816377334496 2216 7 -0.41047273894761815 2216 8 -0.4487442156600281 2216 9 -0.5905113917300042 2216 10-11 -0.6103748554468069 2216 12-13 -0.5758861705775473 2216 14-15 -0.7105326174939535 2216 16-17 -0.7230386433442675 2216 18-19 -0.7229301865198323 2216 20-21 -0.7213530493920786 2216 22-23 -0.7753622970065734 2216 24-25 -0.6990305916198736 2216 26-27 -0.7723110907561122 2216 28-29 -0.8204224456302285 2216 30-31 -0.8478475064662874 2216 32-33 -0.7542126464261969 2216 34-35 -0.7798880939625406 2216 36-37 -0.7233671506173778 2216 38-39 -0.6152309043475199 2216 40-41 -0.6988325941242977 2216 42-43 -0.7167094659126079 2216 44-45 -0.642116921969631 2216 46-47 -0.6660111537280642 2216 48-49 -0.5427249290358276 2216 50-51 -0.6527045985809039 2216 52-53 -0.6423149456881347 2216 54-55 -0.6392780956115942 2216 56-57 -0.5974728697018605 2216 58-59 -0.6528862454427653 2216 60-61 -0.6695439249622623 2216 62-63 -0.6462229461043663 2216 64-65 -0.6179320406379034 2216 66-67 -0.5432266848628089 2216 68-69 -0.6216546952830058 2216 70-71 -0.6441078695660707 2216 72-73 -0.6162650570400388 2216 74-75 -0.5605467828651314 2216 76-77 -0.6087377564427641 2216 78-79 -0.5436937281063479 2216 80-81 -0.5449873617500742 2216 82-83 -0.49867301526099794 2216 84-85 -0.5028251780617481 2216 86-87 -0.5786272067362148 2216 88-89 -0.5729032015626245 2216 90-91 -0.5271499708533227 2216 92-93 -0.4375452743919013 2216 94-95 -0.5599152570940369 2216 96-97 -0.5222443332979161 2216 98-99 -0.5405871547095131 2216 100 -0.6046544556972329 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1669.0 1 469.0 2 331.0 3 316.0 4 244.0 5 314.0 6 383.0 7 803.0 8 2349.0 9 3577.0 10 4021.0 11 5089.0 12 6930.0 13 9481.0 14 13755.0 15 16512.0 16 17560.0 17 19309.0 18 21326.0 19 23772.0 20 26504.0 21 29789.0 22 32485.0 23 38041.0 24 45755.0 25 56607.0 26 59625.0 27 52999.0 28 54559.0 29 68290.0 30 78233.0 31 87986.0 32 110455.0 33 147008.0 34 214016.0 35 329937.0 36 589353.0 37 1243919.0 38 1632088.0 39 562891.0 40 1197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99855966865241 20.65633572710503 13.378487031129499 23.96661757311305 2 14.692404191974045 21.374176653609187 40.51510844083116 23.4183107135856 3 17.517368671471242 27.026371765122292 30.77835803681161 24.677901526594855 4 11.781039326744741 18.22266791597297 40.64415393870454 29.352138818577743 5 12.5658233240966 39.244474194830616 35.675046332341154 12.514656148731632 6 28.78094533937149 39.65781485233555 18.85433240995846 12.706907398334499 7 25.39889073665013 33.799151147880785 23.725183011325104 17.076775104143973 8 24.49508573908029 36.390098542135895 21.247630649687274 17.867185069096536 9 25.704251105384447 16.414455035568885 21.11279827005952 36.76849558898715 10-11 23.477008187691613 27.493793892101586 29.372547550427136 19.656650369779666 12-13 24.26008518654122 25.525806812693517 28.516094333993497 21.698013666771764 14-15 21.891443917810918 26.68014291891985 26.213620898074293 25.214792265194948 16-17 21.523724145620786 29.228950214640133 27.1435446284217 22.103781011317388 18-19 21.381998425784595 29.16222446770325 28.271759817259714 21.184017289252445 20-21 22.794086757443086 28.2980674832043 27.499371412715472 21.408474346637146 22-23 22.512956004485154 28.204339388492468 27.20296674462732 22.07973786239506 24-25 22.09708512355471 28.312917495324196 27.657867733057028 21.932129648064063 26-27 22.058763324194924 28.528371329365974 27.208539186862097 22.204326159577004 28-29 21.926880219548984 28.76156080230717 27.33330782180716 21.978251156336682 30-31 22.38944956604079 28.474334208581414 27.242576039314354 21.89364018606344 32-33 22.220899003277154 28.72036015581394 26.851267306254208 22.207473534654703 34-35 22.402078403291277 28.58797410363595 27.260193550994273 21.749753942078506 36-37 22.397257683282614 28.36878686063042 27.248212990927932 21.985742465159035 38-39 22.292321608900913 28.417174860209606 27.401334922441755 21.88916860844773 40-41 22.359670107234862 28.141749732628718 27.54074529616995 21.957834863966468 42-43 22.13459288816958 28.300160319901142 27.185530653877 22.379716138052284 44-45 22.01553973869924 28.667248714767673 27.38012965571947 21.937081890813616 46-47 22.092191173235648 28.3413916862632 27.130060763686014 22.436356376815137 48-49 22.227699833334313 28.130869585930068 27.35902600580627 22.28240457492935 50-51 22.241184913357866 28.247243399507088 27.05272767027148 22.458844016863562 52-53 21.9472295067269 28.42044034737831 27.08111843006199 22.551211715832796 54-55 21.95211809700216 28.37284509001262 27.17119895836223 22.503837854622986 56-57 22.305990616825643 28.17914156863644 27.091250741689 22.423617072848913 58-59 21.985701140343693 28.13174307231705 27.44531358871651 22.43724219862275 60-61 22.076537969757517 28.42538604255131 27.119366558692636 22.37870942899854 62-63 22.165715154417626 28.103510234436722 27.024547802695448 22.7062268084502 64-65 21.777269518706653 28.18761468839373 27.219735646598085 22.81538014630153 66-67 22.149810280733124 28.081868576638435 27.0773543742137 22.690966768414732 68-69 22.01524348899785 28.072357949881876 27.195888558193975 22.71651000292629 70-71 22.00575028701473 27.875991079614337 27.11926965323767 22.998988980133266 72-73 22.189297615358967 28.043533400132198 27.27503715170251 22.49213183280632 74-75 22.173516444534535 28.256288350892873 27.12028645801735 22.449908746555245 76-77 22.095080325916175 28.10913251008217 27.062182070292323 22.73360509370933 78-79 22.095292529791795 28.15156532460637 27.11391025607281 22.639231889529025 80-81 22.10773656536011 28.059966696667736 27.237001101308223 22.595295636663927 82-83 22.374315719786278 27.70464166267768 27.57807965688625 22.34296296064979 84-85 22.462708798741936 27.95969537329387 27.094125705133347 22.483470122830845 86-87 21.97157786485421 28.08473057639806 27.27613368747178 22.66755787127595 88-89 22.396424335948552 27.683311530685085 27.089996389470745 22.83026774389562 90-91 22.360474311685643 27.649740847217625 27.271860261622265 22.71792457947447 92-93 22.57909521685911 27.706629672050436 27.090048769982833 22.62422634110762 94-95 21.994742699889272 27.682238261711706 27.37632962447398 22.946689413925046 96-97 22.180055492847153 27.638169437220316 27.240265223087828 22.941509846844703 98-99 21.95650986888829 27.98906545878393 27.068271379617265 22.98615329271052 100 22.396886667269445 27.801047045575324 26.743959153143194 23.05810713401204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5717.0 1 3995.0 2 2260.5 3 2207.5 4 2052.5 5 1791.5 6 1596.0 7 1470.0 8 1413.0 9 1391.0 10 1279.0 11 1200.5 12 1185.5 13 1202.5 14 1289.5 15 1444.0 16 1654.0 17 2032.5 18 2601.0 19 3239.5 20 4165.0 21 5606.5 22 7866.0 23 11315.0 24 15932.5 25 21538.0 26 29211.0 27 39207.0 28 48366.5 29 60073.0 30 73628.0 31 85589.5 32 100708.5 33 113083.5 34 125252.5 35 141581.5 36 156041.5 37 171966.5 38 180078.5 39 180000.0 40 181416.5 41 180913.0 42 182502.5 43 189992.5 44 199733.5 45 213468.0 46 227305.0 47 235267.5 48 237640.5 49 230487.0 50 218614.0 51 212848.5 52 204289.5 53 191246.0 54 178765.5 55 160197.0 56 143150.5 57 123831.5 58 105087.5 59 92943.0 60 75200.0 61 56946.0 62 43779.5 63 32779.0 64 22781.5 65 15152.5 66 10834.0 67 7777.0 68 5552.5 69 4316.0 70 3459.5 71 2550.5 72 2013.0 73 1744.5 74 1476.0 75 1154.0 76 874.5 77 667.5 78 470.5 79 317.5 80 239.0 81 155.0 82 123.0 83 98.5 84 76.0 85 63.5 86 46.5 87 38.5 88 34.5 89 25.0 90 14.0 91 11.0 92 13.0 93 17.0 94 15.0 95 10.5 96 9.5 97 8.5 98 7.5 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10126655385514338 2 0.06613253208987535 3 0.08192590767791567 4 0.08383323407511692 5 0.08540187634571236 6 0.09171209638878941 7 0.08852133540655553 8 0.08975129355054512 9 0.07495614486197463 10-11 0.08167635095304822 12-13 0.07156039085574249 14-15 0.08076725145531678 16-17 0.08261218867130117 18-19 0.0822645918045215 20-21 0.07348554273329143 22-23 0.0773893229294323 24-25 0.08012553416280047 26-27 0.07344989177259606 28-29 0.0826656651123442 30-31 0.08680117655300487 32-33 0.08785287989351771 34-35 0.09033062166184458 36-37 0.09003650123610793 38-39 0.09139123774253126 40-41 0.08758549768830258 42-43 0.09296879275330053 44-45 0.08959977696758989 46-47 0.08538405086536469 48-49 0.09472460256754653 50-51 0.09058017838671203 52-53 0.0961595537355344 54-55 0.08783505441317004 56-57 0.09519697779675992 58-59 0.09953748226141886 60-61 0.09827187315673393 62-63 0.09866403372438277 64-65 0.10037527983775961 66-67 0.10241629733756842 68-69 0.09959095870246189 70-71 0.10169436538348757 72-73 0.0991185834732485 74-75 0.10294214900782486 76-77 0.10022376325480437 78-79 0.1071935260707454 80-81 0.10165871442279223 82-83 0.10649833233718609 84-85 0.10296888722834638 86-87 0.09888685222872871 88-89 0.09813818205412636 90-91 0.09859273180299206 92-93 0.09211316969661211 94-95 0.09213990791713363 96-97 0.09056235290636436 98-99 0.10465339512120168 100 0.24294347165846664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5609947.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.51553468276923 #Duplication Level Percentage of deduplicated Percentage of total 1 67.92538747680138 34.312872669248975 2 14.826272402389387 14.979141555181705 3 6.5280217050028995 9.892995205468328 4 3.515645529607334 7.103788547328076 5 2.0569863711581418 5.195488318711136 6 1.315948675718271 3.9885510581394326 7 0.8732179895203998 3.0877751544665037 8 0.648313511723646 2.6199922949427004 9 0.4457805585116304 2.0266958937958655 >10 1.8208586620145975 14.632342515091224 >50 0.03453379100159714 1.1619878660077585 >100 0.0088175978167282 0.7286883515318013 >500 1.438191560370407E-4 0.05525109740056604 >1k 0.0 0.0 >5k 7.190957801842677E-5 0.2144294726858475 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5903 0.10522381049232728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.34764410430259E-5 2 0.0 0.0 0.0 3.2085864625815537E-4 5.34764410430259E-5 3 0.0 0.0 0.0 3.386841266058307E-4 5.34764410430259E-5 4 0.0 0.0 0.0 4.278115283442072E-4 5.34764410430259E-5 5 0.0 0.0 0.0 4.6346248903955775E-4 5.34764410430259E-5 6 0.0 0.0 0.0 4.6346248903955775E-4 7.130192139070119E-5 7 0.0 0.0 0.0 4.6346248903955775E-4 7.130192139070119E-5 8 0.0 0.0 0.0 4.6346248903955775E-4 7.130192139070119E-5 9 0.0 0.0 0.0 5.525898907779343E-4 7.130192139070119E-5 10-11 0.0 0.0 0.0 9.982268994698168E-4 9.804014191221415E-5 12-13 0.0 0.0 0.0 0.0010071396396436544 3.4759686677966837E-4 14-15 0.0 0.0 0.0 0.0010517033405128427 4.4563700869188253E-4 16-17 0.0 0.0 0.0 0.0010962670413820308 4.991134497349084E-4 18-19 0.0 0.0 0.0 0.001140830742251219 5.525898907779343E-4 20-21 0.0 0.0 0.0 0.0012121326636419204 6.417172925163107E-4 22-23 0.0 0.0 0.0 0.0014706021286832122 6.773682532116614E-4 24-25 1.7825480347675298E-5 0.0 0.0 0.0020944939408518476 6.773682532116614E-4 26-27 1.7825480347675298E-5 0.0 0.0 0.003172935501886203 7.575829147762002E-4 28-29 1.7825480347675298E-5 0.0 0.0 0.008476015905319604 7.664956549500378E-4 30-31 1.7825480347675298E-5 0.0 0.0 0.02125688531460279 7.664956549500378E-4 32-33 1.7825480347675298E-5 0.0 0.0 0.047228610181165705 7.664956549500378E-4 34-35 1.7825480347675298E-5 0.0 0.0 0.08840546978429564 8.021466156453884E-4 36-37 1.7825480347675298E-5 0.0 0.0 0.15208699832636566 8.021466156453884E-4 38-39 1.7825480347675298E-5 0.0 0.0 0.24821981384137853 8.467103165145768E-4 40-41 1.7825480347675298E-5 0.0 0.0 0.35898734872183286 8.556230566884144E-4 42-43 1.7825480347675298E-5 0.0 0.0 0.46778516802386905 8.734485370360896E-4 44-45 1.7825480347675298E-5 0.0 0.0 0.6025547121924681 8.91274017383765E-4 46-47 1.7825480347675298E-5 0.0 0.0 0.7683227666856746 9.090994977314403E-4 48-49 1.7825480347675298E-5 0.0 0.0 0.9486631513631056 9.447504584267908E-4 50-51 1.7825480347675298E-5 0.0 0.0 1.1436739063666734 9.625759387744662E-4 52-53 1.7825480347675298E-5 0.0 0.0 1.3355562895692241 0.0010873543012081932 54-55 1.7825480347675298E-5 0.0 0.0 1.5460752124752695 0.0010873543012081932 56-57 1.7825480347675298E-5 0.0 0.0 1.7958725813274172 0.0011051797815558686 58-59 1.7825480347675298E-5 0.0 0.0 2.0812674344338724 0.0011051797815558686 60-61 1.7825480347675298E-5 0.0 0.0 2.383614319350967 0.0011230052619035438 62-63 1.7825480347675298E-5 0.0 0.0 2.690774083961934 0.001194307183294245 64-65 1.7825480347675298E-5 0.0 0.0 3.00687332696726 0.001194307183294245 66-67 1.7825480347675298E-5 0.0 0.0 3.365958715831005 0.0012032199234680825 68-69 1.7825480347675298E-5 0.0 0.0 3.7713547026380105 0.0012121326636419204 70-71 1.7825480347675298E-5 0.0 0.0 4.21333748785862 0.0012299581439895956 72-73 1.7825480347675298E-5 0.0 0.0 4.668217008110771 0.0012745218448587839 74-75 1.7825480347675298E-5 0.0 0.0 5.117347810950799 0.00132799828590181 76-77 1.7825480347675298E-5 0.0 0.0 5.610525375729932 0.0013636492465971604 78-79 1.7825480347675298E-5 8.912740173837649E-6 0.0 6.158837151224423 0.001372561986770998 80-81 1.7825480347675298E-5 1.7825480347675298E-5 0.0 6.75753264692162 0.0013814747269448356 82-83 1.7825480347675298E-5 1.7825480347675298E-5 0.0 7.376629404876731 0.0014438639081616991 84-85 1.7825480347675298E-5 1.7825480347675298E-5 0.0 7.984585237614544 0.0014616893885093746 86-87 1.7825480347675298E-5 1.7825480347675298E-5 0.0 8.610910227850638 0.0015062530893785626 88 1.7825480347675298E-5 1.7825480347675298E-5 0.0 9.110603005696845 0.001550816790247751 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7610 0.0 33.308727 1 GTATCAA 10970 0.0 31.894825 1 CAACGCA 11865 0.0 28.091406 5 ATCAACG 11955 0.0 27.959818 3 TATCAAC 13440 0.0 26.094793 2 AACGCAG 12985 0.0 25.986376 6 TCAACGC 13530 0.0 24.913334 4 GTACATG 18635 0.0 24.529617 1 GGCGTCG 1330 0.0 24.027514 8 ACGCAGA 13895 0.0 23.743124 7 TACATGG 18145 0.0 23.7071 2 CGCAGAG 14080 0.0 23.130346 8 ACATGGG 17860 0.0 23.032494 3 TACGGGC 1535 0.0 21.438782 4 ACGGGCG 1530 0.0 20.586302 5 CATGGGG 12890 0.0 20.570112 4 CGGGCGT 1530 0.0 20.272892 6 ATACGGG 1565 0.0 20.126802 3 GTACTAG 2560 0.0 20.02352 1 GCAGAGT 15945 0.0 20.011578 9 >>END_MODULE