FastQCFastQC Report
Mon 16 Jan 2017
ERR1146515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5609947
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275380.49087807781428244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237650.42362254046250347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141350.25196316471439034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137400.058.3212361
GTATCAA253050.043.3563041
ATCAACG320250.033.8450133
CAACGCA326050.033.2419625
TATCAAC331700.033.0606542
TCAACGC340050.031.8878734
AACGCAG343700.031.8488486
ACGCAGA374700.028.9356257
CGCAGAG376850.028.5335928
GCAGAGT410250.026.0272739
GTGGTAT42400.025.1769941
GTACATG177400.024.1760371
GAGTACT296800.023.57011212-13
TACATGG177400.023.2473982
CAGAGTA399900.022.68784110-11
ACATGGG175800.022.60223
AGAGTAC385100.022.37598410-11
TGGTATC48500.021.9126592
CTGTGCG27900.021.7297369
AGTACTT314900.021.2974712-13
Produced by FastQC (version 0.11.5)