##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5609947
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35627538014174	33.0	31.0	34.0	30.0	34.0
2	32.46156211457969	34.0	31.0	34.0	30.0	34.0
3	32.560320979859526	34.0	31.0	34.0	31.0	34.0
4	36.05952926114988	37.0	35.0	37.0	35.0	37.0
5	36.10243216201508	37.0	35.0	37.0	35.0	37.0
6	36.16320457216441	37.0	36.0	37.0	35.0	37.0
7	36.128105310085814	37.0	36.0	37.0	35.0	37.0
8	36.13602481449468	37.0	36.0	37.0	35.0	37.0
9	37.92213010212039	39.0	38.0	39.0	35.0	39.0
10-11	37.87538928620894	39.0	38.0	39.0	35.0	39.0
12-13	37.83989100075277	39.0	38.0	39.0	35.0	39.0
14-15	39.18043690965351	40.0	39.0	41.0	36.0	41.0
16-17	39.13439913068697	40.0	39.0	41.0	36.0	41.0
18-19	39.25674850404113	40.5	39.0	41.0	36.0	41.0
20-21	39.316234627528566	41.0	39.0	41.0	36.0	41.0
22-23	39.27825966983289	41.0	39.0	41.0	36.0	41.0
24-25	39.26527924417111	41.0	39.0	41.0	36.0	41.0
26-27	39.17974171591995	41.0	39.0	41.0	35.5	41.0
28-29	39.11182939874476	40.5	39.0	41.0	35.0	41.0
30-31	38.94081958350052	40.0	38.5	41.0	35.0	41.0
32-33	38.79662116237462	40.0	38.0	41.0	35.0	41.0
34-35	38.707963016406396	40.0	38.0	41.0	35.0	41.0
36-37	38.62591910404858	40.0	38.0	41.0	35.0	41.0
38-39	38.5239791926733	40.0	38.0	41.0	35.0	41.0
40-41	38.41351896907404	40.0	38.0	41.0	34.0	41.0
42-43	38.29121799189903	40.0	38.0	41.0	34.0	41.0
44-45	38.12323726053027	40.0	38.0	41.0	33.5	41.0
46-47	37.842659297850766	40.0	38.0	41.0	33.0	41.0
48-49	37.52388944137975	40.0	37.0	41.0	32.5	41.0
50-51	37.40488056304275	40.0	37.0	41.0	32.0	41.0
52-53	37.30567169351154	40.0	37.0	41.0	32.0	41.0
54-55	37.15752742405588	40.0	36.5	41.0	31.5	41.0
56-57	37.012600742930374	40.0	36.0	41.0	31.0	41.0
58-59	36.84519675497826	39.0	35.5	41.0	31.0	41.0
60-61	36.65674221164656	39.0	35.0	41.0	31.0	41.0
62-63	36.43475758327129	39.0	35.0	41.0	31.0	41.0
64-65	36.17610638745785	38.5	35.0	41.0	30.5	41.0
66-67	35.9227201255199	37.5	35.0	40.0	30.0	41.0
68-69	35.64089571612709	37.0	35.0	40.0	30.0	41.0
70-71	35.34105277643443	36.5	35.0	39.5	30.0	41.0
72-73	35.02250529283076	36.0	35.0	39.0	29.5	41.0
74-75	34.69930794355098	36.0	35.0	39.0	29.0	40.5
76-77	34.39333535593117	35.0	34.5	37.5	29.0	39.5
78-79	34.099754685739455	35.0	34.0	37.0	29.0	39.0
80-81	33.831542080522325	35.0	34.0	37.0	29.0	39.0
82-83	33.56910920905313	35.0	34.0	36.0	29.0	38.0
84-85	33.34494603959716	35.0	34.0	36.0	29.0	37.0
86-87	33.15101105233258	35.0	34.0	35.5	29.0	37.0
88-89	32.9465249849954	35.0	34.0	35.0	28.0	36.5
90-91	32.85214922707826	35.0	34.0	35.0	28.5	36.0
92-93	32.74381308771723	35.0	34.0	35.0	27.5	36.0
94-95	32.668641343670444	35.0	34.0	35.0	27.0	36.0
96-97	32.61236567832103	35.0	34.0	35.0	27.0	35.5
98-99	32.54161189045101	35.0	34.0	35.0	27.0	35.0
100	31.836725195443023	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.316913206813652
1101	2	0.3282401388469225
1101	3	0.33713487274781784
1101	4	0.21751296561842537
1101	5	0.208521735185073
1101	6	0.26897352888453696
1101	7	0.23333989402567568
1101	8	0.22729295321030207
1101	9	0.28677916265966985
1101	10-11	0.30110538485723737
1101	12-13	0.30857739140339646
1101	14-15	0.4807593081291017
1101	16-17	0.49205946477818685
1101	18-19	0.4528529956929077
1101	20-21	0.40151352225236536
1101	22-23	0.4094202916024159
1101	24-25	0.3994646992587718
1101	26-27	0.39926989802035706
1101	28-29	0.4128700014410285
1101	30-31	0.45276400373680303
1101	32-33	0.434881882411716
1101	34-35	0.4202997203458878
1101	36-37	0.4535372958882462
1101	38-39	0.4776450973851709
1101	40-41	0.46448271289735743
1101	42-43	0.48344986040779503
1101	44-45	0.4468478778096667
1101	46-47	0.42941923751676114
1101	48-49	0.41575418270365105
1101	50-51	0.4030567871553785
1101	52-53	0.34944081130494453
1101	54-55	0.342668237390626
1101	56-57	0.32026958833385066
1101	58-59	0.365908107825355
1101	60-61	0.291521618206005
1101	62-63	0.3082757712351736
1101	64-65	0.27699313109963697
1101	66-67	0.24133395816689074
1101	68-69	0.22008887782806852
1101	70-71	0.27988391688501224
1101	72-73	0.2501784153141813
1101	74-75	0.2684441026648514
1101	76-77	0.2295518403806227
1101	78-79	0.19613388752492256
1101	80-81	0.17545099946926968
1101	82-83	0.16146662544726098
1101	84-85	0.19097209808126792
1101	86-87	0.16452274325088467
1101	88-89	0.25619123744021266
1101	90-91	0.21614686501039415
1101	92-93	0.24487937527086245
1101	94-95	0.22342746132724756
1101	96-97	0.16203175432806916
1101	98-99	0.23588644791788482
1101	100	0.21129606389222744
1102	1	0.282516450104751
1102	2	0.2896438202084042
1102	3	0.28824088397883685
1102	4	0.21593106027678033
1102	5	0.18530671513074282
1102	6	0.20669338194042552
1102	7	0.21669440759744418
1102	8	0.21015812949228518
1102	9	0.2628643362669365
1102	10-11	0.274794470712699
1102	12-13	0.26188289607203785
1102	14-15	0.40537630745373576
1102	16-17	0.5017193426007012
1102	18-19	0.4373391214689022
1102	20-21	0.3803209187572847
1102	22-23	0.37163706952527775
1102	24-25	0.39074768156751105
1102	26-27	0.3333824593325261
1102	28-29	0.3455807783788245
1102	30-31	0.3755163872444882
1102	32-33	0.3755454251130885
1102	34-35	0.3823643031047794
1102	36-37	0.35242108046290355
1102	38-39	0.34888572416678443
1102	40-41	0.3837466447780713
1102	42-43	0.43574090389083864
1102	44-45	0.4173057561112614
1102	46-47	0.49042792978267613
1102	48-49	0.600048549440821
1102	50-51	0.5346890192678941
1102	52-53	0.49236753201098793
1102	54-55	0.49988072806694817
1102	56-57	0.49288922450768524
1102	58-59	0.4721901049815429
1102	60-61	0.49331092994585646
1102	62-63	0.4478918225303019
1102	64-65	0.45567300069755134
1102	66-67	0.42648453987094115
1102	68-69	0.3933951323820182
1102	70-71	0.4585347098655248
1102	72-73	0.4409169140642035
1102	74-75	0.42603887707790733
1102	76-77	0.42265864029052125
1102	78-79	0.39497942609246195
1102	80-81	0.3887451482014086
1102	82-83	0.35007049398308965
1102	84-85	0.360607079340177
1102	86-87	0.38039597773732936
1102	88-89	0.4495595691337897
1102	90-91	0.4018971693496809
1102	92-93	0.37510474643393366
1102	94-95	0.42717959787393767
1102	96-97	0.4361768873914116
1102	98-99	0.4307940027432764
1102	100	0.5140464260506405
1103	1	0.24211468379576928
1103	2	0.25237043827787176
1103	3	0.2511757958587282
1103	4	0.16901699748805754
1103	5	0.14216823283167201
1103	6	0.19077308969555418
1103	7	0.20021725145909386
1103	8	0.19508466879163677
1103	9	0.2265294027762934
1103	10-11	0.22664510208791455
1103	12-13	0.2570657761369617
1103	14-15	0.3828303327017082
1103	16-17	0.4729114515808135
1103	18-19	0.36779424238877567
1103	20-21	0.36641317130455775
1103	22-23	0.3568865469429241
1103	24-25	0.3515977107279369
1103	26-27	0.3530726038112775
1103	28-29	0.35631427141111516
1103	30-31	0.3834410991334636
1103	32-33	0.33491561087048183
1103	34-35	0.3383227186076425
1103	36-37	0.34257743808767316
1103	38-39	0.3379672199120378
1103	40-41	0.2911292929674332
1103	42-43	0.2836233410762432
1103	44-45	0.295814537148992
1103	46-47	0.30856078061678005
1103	48-49	0.3081395567303957
1103	50-51	0.2787426058247462
1103	52-53	0.22589116779739982
1103	54-55	0.24522899721858948
1103	56-57	0.23189877828799865
1103	58-59	0.21028030450786872
1103	60-61	0.22460430322504976
1103	62-63	0.23482100756228874
1103	64-65	0.23898960975315475
1103	66-67	0.18553861566130792
1103	68-69	0.16690556590275918
1103	70-71	0.19196650877717758
1103	72-73	0.17071471983427244
1103	74-75	0.1402261739712074
1103	76-77	0.1298587058266918
1103	78-79	0.1946746934444974
1103	80-81	0.17715306794177366
1103	82-83	0.17516158292890793
1103	84-85	0.2122441814023759
1103	86-87	0.15737465454720478
1103	88-89	0.19038410007674855
1103	90-91	0.2210019318443983
1103	92-93	0.19714373095409599
1103	94-95	0.15439905500775097
1103	96-97	0.13109582773272876
1103	98-99	0.15890540473519366
1103	100	0.18118413543114542
1104	1	0.19324142685911738
1104	2	0.168984927098208
1104	3	0.18529620080584408
1104	4	0.1024928884745222
1104	5	0.1008532805689697
1104	6	0.09607240108362447
1104	7	0.12998164271046875
1104	8	0.11123491494537774
1104	9	0.14478552691362978
1104	10-11	0.1719834953158994
1104	12-13	0.16676945841891921
1104	14-15	0.26863182511471706
1104	16-17	0.3442169073850536
1104	18-19	0.3030088221993168
1104	20-21	0.26490117125897683
1104	22-23	0.2792186470885838
1104	24-25	0.2278720212411045
1104	26-27	0.25836896493550654
1104	28-29	0.26133700404179194
1104	30-31	0.29445520722732965
1104	32-33	0.2943218455172385
1104	34-35	0.27161006399686016
1104	36-37	0.2475275331879061
1104	38-39	0.2561278843630035
1104	40-41	0.22656548167688584
1104	42-43	0.14586443338193078
1104	44-45	0.17385263068442924
1104	46-47	0.2415522179337799
1104	48-49	0.2599026750955318
1104	50-51	0.18642074546839638
1104	52-53	0.11397467233646807
1104	54-55	0.15059002676979105
1104	56-57	0.13816123213163678
1104	58-59	0.10493092680328431
1104	60-61	0.11426446945444724
1104	62-63	0.15023168700194844
1104	64-65	0.12803523941541783
1104	66-67	0.11266140170871353
1104	68-69	0.12237024805693153
1104	70-71	0.13204610757384927
1104	72-73	0.1429497529084287
1104	74-75	0.16674345058061846
1104	76-77	0.11221429685474504
1104	78-79	0.11572047855514711
1104	80-81	0.08660282406685837
1104	82-83	0.05026165452198228
1104	84-85	0.09380505952027107
1104	86-87	0.12191088934859806
1104	88-89	0.18139885112466914
1104	90-91	0.07439874355191733
1104	92-93	0.08345572074495777
1104	94-95	0.0830203377092289
1104	96-97	0.09920961350857738
1104	98-99	0.10609591934530727
1104	100	0.11622454794178338
1105	1	0.18068732534837295
1105	2	0.16923881716144962
1105	3	0.15032182418693907
1105	4	0.12364666288098647
1105	5	0.10458486182064775
1105	6	0.13132837819844667
1105	7	0.1360322109018881
1105	8	0.13093505553835172
1105	9	0.18676012005018094
1105	10-11	0.1781935738776852
1105	12-13	0.2045011346960095
1105	14-15	0.28873289804099045
1105	16-17	0.33936039098503556
1105	18-19	0.2671234930079862
1105	20-21	0.26032668583916774
1105	22-23	0.26678523264350673
1105	24-25	0.2524650142962841
1105	26-27	0.27013250597804017
1105	28-29	0.26847145990806354
1105	30-31	0.2958761720669685
1105	32-33	0.3081453654564683
1105	34-35	0.2969176211006044
1105	36-37	0.2769707288736214
1105	38-39	0.25237377443389875
1105	40-41	0.2419289714439543
1105	42-43	0.27889687946058217
1105	44-45	0.2524820596103652
1105	46-47	0.2747271941694507
1105	48-49	0.3945453715470322
1105	50-51	0.35813518579259096
1105	52-53	0.3153174564473247
1105	54-55	0.30299077383540407
1105	56-57	0.29139309287921833
1105	58-59	0.2976251700804795
1105	60-61	0.21554960853116256
1105	62-63	0.21727589901322375
1105	64-65	0.23908746895728683
1105	66-67	0.2467190540770332
1105	68-69	0.23277292464874932
1105	70-71	0.2158890838082428
1105	72-73	0.22377453209390552
1105	74-75	0.227412054851726
1105	76-77	0.20338231391340145
1105	78-79	0.1956837630290451
1105	80-81	0.2217078686092293
1105	82-83	0.18375620953356986
1105	84-85	0.15636016549433407
1105	86-87	0.15296018203103756
1105	88-89	0.22392981330806094
1105	90-91	0.17282743606273243
1105	92-93	0.17601928812675283
1105	94-95	0.16523369177807723
1105	96-97	0.18029034748746398
1105	98-99	0.2224735184738833
1105	100	0.23375995846275543
1106	1	0.09437969384472922
1106	2	0.04049040429219275
1106	3	0.06742115951315952
1106	4	0.04843890171806464
1106	5	0.0593277836872943
1106	6	0.09989415282289826
1106	7	0.0695648480849087
1106	8	0.08475364895743098
1106	9	0.10318200969022939
1106	10-11	0.10575024271425093
1106	12-13	0.11102184924438774
1106	14-15	0.19999529698672092
1106	16-17	0.2870433519648756
1106	18-19	0.14443994766764234
1106	20-21	0.14147091563446423
1106	22-23	0.10177113236846935
1106	24-25	0.09817855818374
1106	26-27	0.08120925397597745
1106	28-29	0.11737100597071759
1106	30-31	0.08685884795527699
1106	32-33	0.08791488145473636
1106	34-35	0.11337173841299375
1106	36-37	0.12960974670799885
1106	38-39	0.10048684765526161
1106	40-41	0.07419756430540758
1106	42-43	0.04694543011670049
1106	44-45	0.10731476674858698
1106	46-47	0.12870886617989896
1106	48-49	0.1513394445885723
1106	50-51	0.12756643268650691
1106	52-53	0.038581084612552274
1106	54-55	0.0991663215339571
1106	56-57	0.05876910189410012
1106	58-59	0.07953592807319154
1106	60-61	0.04835761578242881
1106	62-63	0.04223534897798231
1106	64-65	0.06897653823831007
1106	66-67	0.072069658916881
1106	68-69	0.08382508672912081
1106	70-71	0.07524626129052336
1106	72-73	0.035420706931198254
1106	74-75	-0.006437015449819228
1106	76-77	-0.016552674644593424
1106	78-79	-0.03485006594384288
1106	80-81	0.04272820939604571
1106	82-83	-0.006448136366898893
1106	84-85	0.008598961317552778
1106	86-87	0.06223425046491826
1106	88-89	0.034915867029198466
1106	90-91	-0.03921983159744258
1106	92-93	0.011661443786024961
1106	94-95	-0.0018232564057214518
1106	96-97	0.004343725495992601
1106	98-99	0.01614178534619981
1106	100	0.09882186171801877
1107	1	0.08041039305413733
1107	2	0.06929411354880699
1107	3	0.08126874348311475
1107	4	0.06922414909235641
1107	5	0.0636879029268016
1107	6	0.08927841419325944
1107	7	0.08889515940254
1107	8	0.05238713401674033
1107	9	0.09820998502176082
1107	10-11	0.09649859211303635
1107	12-13	0.11174042784200111
1107	14-15	0.18051562369576857
1107	16-17	0.23837967761735257
1107	18-19	0.17195283343475154
1107	20-21	0.11293574533446105
1107	22-23	0.11824197757553634
1107	24-25	0.13835067709970872
1107	26-27	0.1274646740865606
1107	28-29	0.1530121527424555
1107	30-31	0.20637275043880976
1107	32-33	0.1335665040786722
1107	34-35	0.11218299684769306
1107	36-37	0.12906104415460362
1107	38-39	0.10948656592868389
1107	40-41	0.12773938702554233
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2216	52-53	-0.5563681343318478
2216	54-55	-0.5306303569464035
2216	56-57	-0.48726232481458
2216	58-59	-0.5688801535723869
2216	60-61	-0.5783493417625536
2216	62-63	-0.5224558107694435
2216	64-65	-0.42916410952050654
2216	66-67	-0.5182893008653053
2216	68-69	-0.48608167153174264
2216	70-71	-0.4343427435255691
2216	72-73	-0.43192443238941536
2216	74-75	-0.4600089632942783
2216	76-77	-0.3767254285572932
2216	78-79	-0.35477563707519266
2216	80-81	-0.379009390496698
2216	82-83	-0.3714303814737434
2216	84-85	-0.39832613175102694
2216	86-87	-0.3342206503609617
2216	88-89	-0.26520727197054583
2216	90-91	-0.34348072277553854
2216	92-93	-0.3992364609824577
2216	94-95	-0.3676285558926864
2216	96-97	-0.38399340430014917
2216	98-99	-0.392560398627289
2216	100	-0.441860431074403
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	32.0
8	221.0
9	596.0
10	776.0
11	515.0
12	432.0
13	618.0
14	1014.0
15	1978.0
16	3515.0
17	6128.0
18	10686.0
19	15498.0
20	22370.0
21	32666.0
22	45791.0
23	52293.0
24	47676.0
25	38791.0
26	34429.0
27	36813.0
28	44541.0
29	54143.0
30	65890.0
31	83740.0
32	106246.0
33	138077.0
34	198794.0
35	309525.0
36	533083.0
37	1227345.0
38	1803364.0
39	690243.0
40	2114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27502625316878	20.31183291950389	13.87641517993782	23.536725647389506
2	15.07942916886152	21.32962191483881	40.27693028968495	23.314018626614722
3	17.384805788440836	27.137639108018824	31.519308855229976	23.958246248310363
4	11.660925226691168	18.383867278928403	40.181834459625755	29.773373034754673
5	11.996551756101631	38.7417261041903	35.84052371095502	13.421198428753048
6	28.2408668456908	39.93390344390902	18.379488141196134	13.445741569204046
7	24.562121531629444	34.305190405542156	23.714288209852963	17.418399852975437
8	25.027692007125946	35.02424810897549	21.225143593796847	18.722916290101715
9	26.17327840942412	14.796549626261799	21.423711435214226	37.60646052909985
10-11	23.690262751379603	26.697343751504025	29.295793075935546	20.316600421180826
12-13	25.192492905904455	24.873684189886287	28.98126310284215	20.95255980136711
14-15	22.2745192199711	25.56007669236032	27.800882575340296	24.36452151232828
16-17	20.729929219227877	28.300151829056613	28.584807914184275	22.38511103753123
18-19	20.508770464692425	27.043380972315816	31.59807602100309	20.849772541988663
20-21	21.766095801313863	26.19965994249308	31.36514898082125	20.669095275371802
22-23	21.466049501002416	26.157662452069513	31.126657702826783	21.249630344101288
24-25	21.08541310639833	26.26876867107657	31.567143147698186	21.07867507482691
26-27	21.09822831296367	26.495295712401685	31.11679269752745	21.289683277107198
28-29	20.94288947827849	26.693968766549848	31.231293272467635	21.131848482704026
30-31	21.45048535313059	26.39432963228101	31.10268994156083	21.052495073027572
32-33	21.40868258683108	26.60186727836776	30.649779110414844	21.339671024386313
34-35	21.504884321836272	26.55315623279003	30.982945538004465	20.959013907369233
36-37	21.611351487884992	26.337798797599195	30.812755138510322	21.238094576005494
38-39	21.569783319084735	26.3816536741779	30.92745014713254	21.121112859604825
40-41	21.628821946553778	26.224083964128187	30.865478550984555	21.281615538333483
42-43	21.60603299817271	26.36478562096932	30.317175902018324	21.71200547883964
44-45	21.544722294787203	26.81613953296436	30.20505036217575	21.434087810072686
46-47	21.80565163984615	26.583673606898607	29.56888897524344	22.041785778011807
48-49	22.15888728330783	26.673280515826757	29.313956793341394	21.85387540752401
50-51	22.086420780802385	26.998561661990745	28.755004280789105	22.160013276417764
52-53	21.769333862513985	27.242886520608412	28.74261989520191	22.245159721675698
54-55	21.81310834654874	27.223541757521197	28.684032482048128	22.279317413881937
56-57	22.138779116046354	26.97450517995413	28.600305867608117	22.2864098363914
58-59	21.702532528388392	26.93238703133697	29.02024676877471	22.34483367149993
60-61	21.82764719802029	27.25315479238331	28.73707144534657	22.18212656424983
62-63	21.816598103866312	26.946009423983348	28.64106259922117	22.596329872929168
64-65	21.403600401431035	26.919507017021605	28.877775658519806	22.799116923027558
66-67	21.7035767718125	26.82880113000413	28.760772713765743	22.70684938441763
68-69	21.59260224829261	26.7292623416558	28.85329946410865	22.824835945942933
70-71	21.588460537115814	26.523254075910756	28.765364854832875	23.122920532140554
72-73	21.74210776348826	26.616305259470728	28.850642226038964	22.790944751002044
74-75	21.714749953095456	26.917540249893158	28.545914536130763	22.821795260880627
76-77	21.690648565694133	26.788401268727103	28.358997432490803	23.16195273308796
78-79	21.572894607371282	26.899871298494798	28.4745930394233	23.05264105471062
80-81	21.563674183251756	26.847678744130782	28.503764828627904	23.08488224398956
82-83	21.761297732280504	26.539485756364318	28.8095047531074	22.88971175824777
84-85	21.849056835842298	26.710739788690375	28.308249342971852	23.131954032495475
86-87	21.32810702133193	26.889336031160365	28.443602051855393	23.33895489565231
88-89	21.675908881135598	26.546150970766746	28.248519994930433	23.529420153167223
90-91	21.543685228314608	26.477030837465353	28.420686083314	23.55859785090604
92-93	21.670014441316766	26.590686130551532	28.22275189660933	23.516547531522363
94-95	21.096961723569084	26.516234886983202	28.49335924329322	23.89344414615449
96-97	21.257328415978353	26.416852342004837	28.384453878317302	23.941365363699507
98-99	21.04242976508757	26.666632798220803	28.251043259542012	24.039894177149616
100	21.384044415695342	26.371444484127082	28.04099339480297	24.2035177053746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	524.0
1	369.5
2	856.0
3	1416.5
4	890.5
5	679.0
6	1492.0
7	3865.5
8	7235.0
9	8620.0
10	6178.5
11	3621.0
12	3470.5
13	3863.5
14	4461.0
15	5224.5
16	6038.0
17	7054.0
18	8370.0
19	9618.0
20	10894.5
21	12486.0
22	14639.0
23	17825.0
24	22045.0
25	27192.5
26	34198.0
27	43444.5
28	51953.0
29	62956.5
30	75611.5
31	86650.5
32	101240.5
33	112615.5
34	123194.0
35	138179.0
36	151323.0
37	166872.5
38	175592.5
39	175986.0
40	177441.0
41	176366.0
42	177814.0
43	185088.5
44	194729.0
45	209069.5
46	222496.0
47	230696.5
48	234282.5
49	227933.0
50	215659.0
51	209360.5
52	201006.5
53	187779.0
54	175862.5
55	157617.5
56	140836.5
57	122242.5
58	104086.5
59	92336.5
60	74423.5
61	55477.0
62	42024.0
63	31450.5
64	21659.5
65	14179.5
66	10013.0
67	7045.0
68	5029.5
69	3876.5
70	3072.0
71	2288.5
72	1800.5
73	1618.0
74	1333.5
75	982.5
76	776.0
77	577.0
78	371.5
79	240.0
80	173.5
81	114.5
82	76.5
83	59.5
84	38.5
85	21.0
86	14.5
87	10.0
88	7.0
89	3.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026381710914559443
2	0.004135511440660669
3	1.426038427814024E-4
4	0.04918050027923615
5	0.0024064398469361655
6	0.004902007095610708
7	0.0
8	4.456370086918824E-4
9	7.13019213907012E-5
10-11	8.91274017383765E-5
12-13	0.0
14-15	1.78254803476753E-5
16-17	3.475968667796683E-4
18-19	1.8716754365059063E-4
20-21	2.4064398469361652E-4
22-23	0.0
24-25	0.0
26-27	2.049930239982659E-4
28-29	0.0
30-31	1.960802838244283E-4
32-33	3.1194590608431776E-4
34-35	1.3369110260756474E-4
36-37	7.13019213907012E-5
38-39	6.951937335593366E-4
40-41	4.902007095610707E-4
42-43	0.0
44-45	5.793281112994471E-4
46-47	0.0
48-49	2.673822052151295E-5
50-51	0.0
52-53	2.9412042573664243E-4
54-55	3.1194590608431776E-4
56-57	6.238918121686355E-5
58-59	7.041064737331742E-4
60-61	5.258516702564214E-4
62-63	8.91274017383765E-5
64-65	3.0303316591048004E-4
66-67	9.982268994698168E-4
68-69	0.0023975271067623277
70-71	0.0033601030455367937
72-73	0.0028877278163233983
74-75	0.0026649093119774574
76-77	0.004099860479965319
78-79	0.0011943071832942452
80-81	0.0011319180020773814
82-83	6.773682532116613E-4
84-85	4.902007095610707E-4
86-87	0.0
88-89	0.0
90-91	2.673822052151295E-5
92-93	2.673822052151295E-5
94-95	1.1586562225988945E-4
96-97	3.6542234712734365E-4
98-99	9.804014191221415E-5
100	0.07773691979621197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5609947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.61756890995946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.38330182224595	30.94628147169188
2	15.36396025140115	14.324609514991424
3	6.89395651625332	9.641384788761096
4	3.6024180991784753	6.717438959237509
5	2.1911274000959433	5.107251628223647
6	1.4035098126863785	3.9256929245226924
7	0.9666113336760197	3.154274931975276
8	0.6462105319351393	2.409981120226233
9	0.4964128012073469	2.0827402171262546
>10	1.987863232591025	14.792582963639509
>50	0.04382533741237928	1.3739200235012108
>100	0.017775708010508675	1.5856114195095663
>500	0.0013622189878958215	0.43499786213674574
>1k	0.001437897820503899	1.1946036179547648
>5k	0.0	0.0
>10k+	2.2703649797429986E-4	2.3086285565022857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27538	0.49087807781428244	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23765	0.42362254046250347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14135	0.25196316471439034	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.34764410430259E-5
2	0.0	0.0	0.0	3.030331659104801E-4	5.34764410430259E-5
3	0.0	0.0	0.0	3.030331659104801E-4	5.34764410430259E-5
4	0.0	0.0	0.0	4.278115283442072E-4	5.34764410430259E-5
5	0.0	0.0	0.0	4.991134497349084E-4	5.34764410430259E-5
6	0.0	0.0	0.0	4.991134497349084E-4	8.91274017383765E-5
7	0.0	0.0	0.0	5.169389300825836E-4	8.91274017383765E-5
8	0.0	0.0	0.0	5.169389300825836E-4	8.91274017383765E-5
9	0.0	0.0	0.0	5.525898907779343E-4	8.91274017383765E-5
10-11	0.0	0.0	0.0	8.467103165145768E-4	8.91274017383765E-5
12-13	0.0	0.0	0.0	8.556230566884144E-4	2.2281850434594124E-4
14-15	0.0	0.0	0.0	9.804014191221414E-4	2.9412042573664243E-4
16-17	0.0	0.0	0.0	0.0010071396396436544	3.832478274750189E-4
18-19	0.0	0.0	0.0	0.0010784415610343556	3.921605676488566E-4
20-21	8.912740173837649E-6	0.0	0.0	0.0011764817029465697	4.991134497349084E-4
22-23	1.7825480347675298E-5	0.0	0.0	0.0014349511679878615	5.704153711256095E-4
24-25	1.7825480347675298E-5	0.0	0.0	0.0020766684605041724	5.704153711256095E-4
26-27	1.7825480347675298E-5	0.0	0.0	0.0032085864625815536	6.595427728639861E-4
28-29	1.7825480347675298E-5	0.0	0.0	0.00856514330705798	6.595427728639861E-4
30-31	1.7825480347675298E-5	0.0	0.0	0.021390576417210358	6.595427728639861E-4
32-33	1.7825480347675298E-5	0.0	0.0	0.047433603205163974	6.773682532116614E-4
34-35	1.7825480347675298E-5	0.0	0.0	0.08894023419472591	7.308446942546873E-4
36-37	1.7825480347675298E-5	0.0	0.0	0.15265741369749125	7.308446942546873E-4
38-39	1.7825480347675298E-5	0.0	0.0	0.2490130477168501	7.397574344285249E-4
40-41	1.7825480347675298E-5	0.0	0.0	0.35972710615626136	7.664956549500378E-4
42-43	1.7825480347675298E-5	0.0	0.0	0.46848036175742835	7.932338754715508E-4
44-45	1.7825480347675298E-5	0.0	0.0	0.6029023090592478	8.199720959930637E-4
46-47	1.7825480347675298E-5	0.0	0.0	0.7683138539455008	8.199720959930637E-4
48-49	1.7825480347675298E-5	0.0	0.0	0.9483690309373689	8.823612772099272E-4
50-51	1.7825480347675298E-5	8.912740173837649E-6	0.0	1.1439769395325838	8.91274017383765E-4
52-53	1.7825480347675298E-5	1.7825480347675298E-5	0.0	1.3373923140450348	9.269249780791155E-4
54-55	1.7825480347675298E-5	1.7825480347675298E-5	0.0	1.5489985912522881	9.447504584267908E-4
56-57	1.7825480347675298E-5	1.7825480347675298E-5	0.0	1.8009706687068523	9.536631986006286E-4
58-59	1.7825480347675298E-5	1.7825480347675298E-5	0.0	2.0890660820859805	9.625759387744662E-4
60-61	1.7825480347675298E-5	1.7825480347675298E-5	0.0	2.394015487133836	9.982268994698168E-4
62-63	1.7825480347675298E-5	1.7825480347675298E-5	0.0	2.7044105764279056	0.001069528820860518
64-65	1.7825480347675298E-5	1.7825480347675298E-5	0.0	3.024119479203636	0.001069528820860518
66-67	1.7825480347675298E-5	1.7825480347675298E-5	0.0	3.3867610513967423	0.001069528820860518
68-69	1.7825480347675298E-5	1.7825480347675298E-5	0.0	3.7966757974718837	0.001069528820860518
70-71	1.7825480347675298E-5	1.7825480347675298E-5	0.0	4.243212992921324	0.0010962670413820308
72-73	1.7825480347675298E-5	1.7825480347675298E-5	0.0	4.702245850094483	0.001167568962772732
74-75	1.7825480347675298E-5	1.7825480347675298E-5	0.0	5.155529989855519	0.0012388708841634332
76-77	1.7825480347675298E-5	1.7825480347675298E-5	0.0	5.652361778105925	0.001301260065380297
78-79	1.7825480347675298E-5	1.7825480347675298E-5	0.0	6.203677147039	0.0013369110260756474
80-81	1.7825480347675298E-5	1.7825480347675298E-5	0.0	6.804868209984871	0.0013369110260756474
82-83	1.7825480347675298E-5	1.7825480347675298E-5	0.0	7.426656615472481	0.0013547365064233228
84-85	1.7825480347675298E-5	1.7825480347675298E-5	0.0	8.035976097456892	0.001372561986770998
86-87	1.7825480347675298E-5	1.7825480347675298E-5	0.0	8.6646807893194	0.0014884276090308874
88	1.7825480347675298E-5	1.7825480347675298E-5	0.0	9.16570156545151	0.0016042932312907768
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13740	0.0	58.321236	1
GTATCAA	25305	0.0	43.356304	1
ATCAACG	32025	0.0	33.845013	3
CAACGCA	32605	0.0	33.241962	5
TATCAAC	33170	0.0	33.060654	2
TCAACGC	34005	0.0	31.887873	4
AACGCAG	34370	0.0	31.848848	6
ACGCAGA	37470	0.0	28.935625	7
CGCAGAG	37685	0.0	28.533592	8
GCAGAGT	41025	0.0	26.027273	9
GTGGTAT	4240	0.0	25.176994	1
GTACATG	17740	0.0	24.176037	1
GAGTACT	29680	0.0	23.570112	12-13
TACATGG	17740	0.0	23.247398	2
CAGAGTA	39990	0.0	22.687841	10-11
ACATGGG	17580	0.0	22.6022	3
AGAGTAC	38510	0.0	22.375984	10-11
TGGTATC	4850	0.0	21.912659	2
CTGTGCG	2790	0.0	21.729736	9
AGTACTT	31490	0.0	21.29747	12-13
>>END_MODULE