FastQCFastQC Report
Mon 16 Jan 2017
ERR1146514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5126056
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57310.11180135371131335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79550.033.7572561
GTATCAA120150.030.2570721
CAACGCA135250.025.7212285
ATCAACG136350.025.5491983
GTACATG169000.024.9062041
GTACTAG18200.024.8068161
TATCAAC145200.024.7040352
AACGCAG144900.024.553566
TCAACGC144050.024.1832664
TACATGG169800.023.7552472
ACATGGG176950.021.971983
ACGCAGA159050.021.777937
CGCAGAG161150.021.3478958
CATGGGG124100.019.7367614
TCTAACG8350.019.7056872
TAACGCC8950.019.4351834
CTAACGC8500.018.8050373
TGATCGC11600.018.2308799
GCAGAGT179950.018.1242779
GTATAAG27450.017.8181321
Produced by FastQC (version 0.11.5)