##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146514_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5126056
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.987989401598423	31.0	31.0	34.0	28.0	34.0
2	31.167466957052362	31.0	31.0	34.0	28.0	34.0
3	31.272132415252585	31.0	31.0	34.0	28.0	34.0
4	34.7226126675167	37.0	35.0	37.0	32.0	37.0
5	34.80411958043377	37.0	35.0	37.0	32.0	37.0
6	34.97891439344401	37.0	35.0	37.0	32.0	37.0
7	34.937409774688376	37.0	35.0	37.0	32.0	37.0
8	34.87586226135649	37.0	35.0	37.0	32.0	37.0
9	36.463457480761036	39.0	37.0	39.0	32.0	39.0
10-11	36.45321832223448	39.0	37.0	39.0	32.0	39.0
12-13	36.40123459439382	39.0	37.0	39.0	32.0	39.0
14-15	37.74779099174882	40.0	38.0	41.0	32.0	41.0
16-17	37.77394423705086	40.0	38.0	41.0	32.0	41.0
18-19	37.801851169788236	40.0	38.0	41.0	32.0	41.0
20-21	37.9099112456048	40.0	38.0	41.0	32.0	41.0
22-23	37.94162188239848	40.0	38.0	41.0	32.0	41.0
24-25	37.97953465588358	40.0	38.0	41.0	32.0	41.0
26-27	38.05511381459742	40.0	38.0	41.0	32.0	41.0
28-29	38.04485124626028	40.0	38.0	41.0	32.0	41.0
30-31	38.01130762129793	40.0	38.0	41.0	32.0	41.0
32-33	37.98311518641232	40.0	38.0	41.0	31.5	41.0
34-35	37.93585185179405	40.0	38.0	41.0	31.0	41.0
36-37	37.85597826086956	40.0	38.0	41.0	31.0	41.0
38-39	37.7951688393572	40.0	38.0	41.0	31.0	41.0
40-41	37.742896585601095	40.0	38.0	41.0	31.0	41.0
42-43	37.58474926922375	40.0	37.0	41.0	30.0	41.0
44-45	37.47271245183431	40.0	37.0	41.0	30.0	41.0
46-47	37.36595025493284	40.0	37.0	41.0	30.0	41.0
48-49	37.25496336754807	40.0	36.5	41.0	30.0	41.0
50-51	37.12743930226279	40.0	36.0	41.0	30.0	41.0
52-53	36.996749937964	40.0	36.0	41.0	30.0	41.0
54-55	36.87535241519016	39.0	35.0	41.0	30.0	41.0
56-57	36.69189050997492	39.0	35.0	41.0	30.0	41.0
58-59	36.50070473283944	39.0	35.0	41.0	30.0	41.0
60-61	36.28134680932085	38.5	35.0	41.0	29.0	41.0
62-63	36.04163103173278	38.0	35.0	40.0	29.0	41.0
64-65	35.741674691029516	37.0	35.0	40.0	29.0	41.0
66-67	35.496278425362505	37.0	34.5	40.0	29.0	41.0
68-69	35.230322200927965	36.5	34.0	39.5	29.0	41.0
70-71	34.93323209890801	36.0	34.0	39.0	28.0	41.0
72-73	34.590415906498095	35.0	34.0	39.0	28.0	40.5
74-75	34.2614079908608	35.0	34.0	37.5	28.0	40.0
76-77	33.98727784089756	35.0	34.0	37.0	28.0	39.0
78-79	33.67880423077703	35.0	34.0	37.0	27.0	39.0
80-81	33.44258461085872	35.0	33.5	36.0	27.0	39.0
82-83	33.183563152646016	35.0	33.0	36.0	27.0	37.0
84-85	32.98523484721977	35.0	33.0	35.5	27.0	37.0
86-87	32.794409483626396	35.0	33.0	35.0	26.0	36.5
88-89	32.62314048071266	35.0	33.0	35.0	26.0	36.0
90-91	32.489136579857885	35.0	33.0	35.0	26.0	36.0
92-93	32.35527557638855	35.0	33.0	35.0	25.5	36.0
94-95	32.2378849353187	35.0	33.0	35.0	25.0	35.5
96-97	32.15205774185846	35.0	33.0	35.0	25.0	35.0
98-99	32.05270309181172	35.0	33.0	35.0	25.0	35.0
100	30.931764498866183	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24638115443899622
1101	2	0.29104585907100855
1101	3	0.25746419713413005
1101	4	0.307804422766516
1101	5	0.30678176566011217
1101	6	0.33152913929173167
1101	7	0.3707963396623981
1101	8	0.3834205137463158
1101	9	0.5078672801251045
1101	10-11	0.5499066086529325
1101	12-13	0.5446591523077515
1101	14-15	0.5715557607573913
1101	16-17	0.5404036402553842
1101	18-19	0.5457714625601184
1101	20-21	0.5416060679591865
1101	22-23	0.5199056970045532
1101	24-25	0.5941521339987901
1101	26-27	0.616133341473919
1101	28-29	0.5861499733383582
1101	30-31	0.6326758004246642
1101	32-33	0.60831968477072
1101	34-35	0.5903712978785265
1101	36-37	0.5719181644239058
1101	38-39	0.5999333887868588
1101	40-41	0.5889701419529061
1101	42-43	0.6214432719688574
1101	44-45	0.566831967569513
1101	46-47	0.504620062648975
1101	48-49	0.5349619137280683
1101	50-51	0.5371333765777067
1101	52-53	0.4647176985176955
1101	54-55	0.4820470973392261
1101	56-57	0.5348824295068297
1101	58-59	0.558640422009212
1101	60-61	0.5122605818011507
1101	62-63	0.48067692653448546
1101	64-65	0.5316142142065345
1101	66-67	0.47739956775182435
1101	68-69	0.48194701848525767
1101	70-71	0.4801218361581405
1101	72-73	0.5147395206698704
1101	74-75	0.4817672228285659
1101	76-77	0.4220048323863992
1101	78-79	0.47114447571546947
1101	80-81	0.4105511485747684
1101	82-83	0.43944029756365666
1101	84-85	0.4311078931746479
1101	86-87	0.4347775932732034
1101	88-89	0.44699079711959655
1101	90-91	0.42213172561609014
1101	92-93	0.4328239121794084
1101	94-95	0.4444773722349069
1101	96-97	0.4755361224214738
1101	98-99	0.49347514448109564
1101	100	0.5298775523948827
1102	1	0.16813023364833413
1102	2	0.22605964004450385
1102	3	0.2296484196603501
1102	4	0.23348450501058693
1102	5	0.16143386440391083
1102	6	0.2744169065344195
1102	7	0.2853861263923818
1102	8	0.3356741765051936
1102	9	0.4367460756957229
1102	10-11	0.3655017587685805
1102	12-13	0.3751535526875216
1102	14-15	0.510762798825219
1102	16-17	0.39922016873793353
1102	18-19	0.41105580136915876
1102	20-21	0.4508633554772459
1102	22-23	0.4404576921944994
1102	24-25	0.4968624295578081
1102	26-27	0.4170484415422848
1102	28-29	0.46561362728429856
1102	30-31	0.5196697586601999
1102	32-33	0.5305816662807814
1102	34-35	0.5045797773141345
1102	36-37	0.5097871639349663
1102	38-39	0.4976095669833498
1102	40-41	0.4328383762287089
1102	42-43	0.47335770788490095
1102	44-45	0.38872776678735477
1102	46-47	0.3992927673363056
1102	48-49	0.346103611786603
1102	50-51	0.3406296481149198
1102	52-53	0.3111101983136564
1102	54-55	0.25326552857700335
1102	56-57	0.32221984509322255
1102	58-59	0.31304295821749406
1102	60-61	0.32395655128757284
1102	62-63	0.32071449194825163
1102	64-65	0.3436699640659526
1102	66-67	0.27231708033291113
1102	68-69	0.3239248146825986
1102	70-71	0.25170304281883205
1102	72-73	0.2408652289055837
1102	74-75	0.25349818198581886
1102	76-77	0.2572922486092324
1102	78-79	0.23458232880378205
1102	80-81	0.22613993420930711
1102	82-83	0.2638105695965507
1102	84-85	0.20987516057345346
1102	86-87	0.15064207325391976
1102	88-89	0.15347478071571174
1102	90-91	0.14606075191786516
1102	92-93	0.14027922790634761
1102	94-95	0.1659908907671479
1102	96-97	0.2237742842994308
1102	98-99	0.2825579254149062
1102	100	0.2007230624780938
1103	1	0.161536174662249
1103	2	0.16188099970695546
1103	3	0.1966498543805777
1103	4	0.27576493274096947
1103	5	0.18858046736805534
1103	6	0.2782758092208084
1103	7	0.35654330246718047
1103	8	0.3019243266914202
1103	9	0.4894031351553352
1103	10-11	0.4040292976226425
1103	12-13	0.47324530410634225
1103	14-15	0.5535149880103987
1103	16-17	0.5888608525976835
1103	18-19	0.5275084379326174
1103	20-21	0.4244683904264619
1103	22-23	0.4056826888373948
1103	24-25	0.45659379056309746
1103	26-27	0.45718951058554325
1103	28-29	0.3865753845692197
1103	30-31	0.4296043128014446
1103	32-33	0.4207707949179422
1103	34-35	0.4115480650497503
1103	36-37	0.39927942846062336
1103	38-39	0.3859335593220834
1103	40-41	0.3644644160474897
1103	42-43	0.4063032662596413
1103	44-45	0.3429149589055811
1103	46-47	0.37732423223513223
1103	48-49	0.42793178460324555
1103	50-51	0.42838399291423457
1103	52-53	0.387468765289654
1103	54-55	0.3408127930673217
1103	56-57	0.3553208903893861
1103	58-59	0.4559513410828302
1103	60-61	0.450726098467257
1103	62-63	0.4412719832374492
1103	64-65	0.3708017250966904
1103	66-67	0.3784036865481397
1103	68-69	0.3673816423253413
1103	70-71	0.3092650078908221
1103	72-73	0.3739825296526291
1103	74-75	0.3473430201734118
1103	76-77	0.32471597167289445
1103	78-79	0.35033880801447737
1103	80-81	0.32547946817260254
1103	82-83	0.2801522450387992
1103	84-85	0.3000816047995869
1103	86-87	0.28061783142934615
1103	88-89	0.2577587987804577
1103	90-91	0.33360448261452547
1103	92-93	0.37555744348189535
1103	94-95	0.388560170792033
1103	96-97	0.3737979607170132
1103	98-99	0.3387876502737299
1103	100	0.44303953490390313
1104	1	0.0969622688229741
1104	2	0.13028964610468563
1104	3	0.10858638787546582
1104	4	0.1910727965970338
1104	5	0.13469476338508457
1104	6	0.22781052344043218
1104	7	0.28002943464779406
1104	8	0.28681003107774217
1104	9	0.27994709055437283
1104	10-11	0.33088450139768355
1104	12-13	0.3391811857856837
1104	14-15	0.4202419220693301
1104	16-17	0.32122702369361633
1104	18-19	0.37387367890067225
1104	20-21	0.38969512018801566
1104	22-23	0.3679888735423944
1104	24-25	0.42339389253028514
1104	26-27	0.35452477086202094
1104	28-29	0.35672297884146786
1104	30-31	0.3637958699347337
1104	32-33	0.3948490264971909
1104	34-35	0.4085483824871474
1104	36-37	0.315595408146244
1104	38-39	0.25304430221836327
1104	40-41	0.2841297434537111
1104	42-43	0.33843252902640586
1104	44-45	0.29557913393740876
1104	46-47	0.2811049640674881
1104	48-49	0.29190762144607163
1104	50-51	0.3189063891914614
1104	52-53	0.2862885395017116
1104	54-55	0.23313401470517903
1104	56-57	0.31557889801998584
1104	58-59	0.280847004210365
1104	60-61	0.2560906896365296
1104	62-63	0.28813286216391987
1104	64-65	0.31765038248170896
1104	66-67	0.27067949084052145
1104	68-69	0.2362839300930304
1104	70-71	0.24711776372740957
1104	72-73	0.30744365809216134
1104	74-75	0.2421760307585572
1104	76-77	0.22385774115041812
1104	78-79	0.2517903278216451
1104	80-81	0.23171318034682997
1104	82-83	0.18557443475987867
1104	84-85	0.1787283131109234
1104	86-87	0.12920832064998677
1104	88-89	0.1679744343671672
1104	90-91	0.170900170104467
1104	92-93	0.13317745357374378
1104	94-95	0.1357672863912711
1104	96-97	0.14312755874930616
1104	98-99	0.21758928578218928
1104	100	0.1791510840992352
1105	1	0.09284978985061443
1105	2	0.10274157699569031
1105	3	0.0573002131004543
1105	4	0.1655655173805073
1105	5	0.18036497183209832
1105	6	0.2216270576424435
1105	7	0.22860647634866638
1105	8	0.2649572049998312
1105	9	0.30629512417612403
1105	10-11	0.3304109674490334
1105	12-13	0.31787826380714534
1105	14-15	0.4511285859566314
1105	16-17	0.41472981800232844
1105	18-19	0.38032398181024263
1105	20-21	0.3715794181807155
1105	22-23	0.3872217060201919
1105	24-25	0.40294883265101333
1105	26-27	0.3351112249547654
1105	28-29	0.31942713293290836
1105	30-31	0.3357954710543112
1105	32-33	0.2962139434817601
1105	34-35	0.306242128780319
1105	36-37	0.2946179615099638
1105	38-39	0.2098223081767543
1105	40-41	0.22639640640623782
1105	42-43	0.28185406861587126
1105	44-45	0.24633041334713823
1105	46-47	0.23371038979313852
1105	48-49	0.23183216890757308
1105	50-51	0.27952233172084817
1105	52-53	0.28327522499503743
1105	54-55	0.19969935778642167
1105	56-57	0.1999343780337952
1105	58-59	0.1643835777292466
1105	60-61	0.22433666371039607
1105	62-63	0.2213627612476401
1105	64-65	0.24551839472996306
1105	66-67	0.23526623254539913
1105	68-69	0.22288932741639655
1105	70-71	0.24564782851972922
1105	72-73	0.25792533038739407
1105	74-75	0.2856975557489321
1105	76-77	0.2706201500417009
1105	78-79	0.1971953650602316
1105	80-81	0.22675329506084552
1105	82-83	0.2862919357397189
1105	84-85	0.21338251257969176
1105	86-87	0.1494809380944062
1105	88-89	0.2335782080006581
1105	90-91	0.21446098231925248
1105	92-93	0.223515226948642
1105	94-95	0.18944274473507505
1105	96-97	0.21493395411642524
1105	98-99	0.19886141313116212
1105	100	0.24035410338680663
1106	1	0.13162001764097653
1106	2	0.11423184029939293
1106	3	0.08931330765150491
1106	4	0.16079767993441862
1106	5	0.1262603286635624
1106	6	0.1504393661632193
1106	7	0.21833177811621596
1106	8	0.19383399420756575
1106	9	0.29091142431595074
1106	10-11	0.3012761748222701
1106	12-13	0.30937620288282375
1106	14-15	0.34909544066977816
1106	16-17	0.30437906745426346
1106	18-19	0.35534176793630223
1106	20-21	0.3117547170985091
1106	22-23	0.3114537282812435
1106	24-25	0.3043472906151763
1106	26-27	0.3609639187222271
1106	28-29	0.2722427195565089
1106	30-31	0.30339806260385416
1106	32-33	0.31735238049617465
1106	34-35	0.2992643034953275
1106	36-37	0.2999827846818519
1106	38-39	0.2813643526426475
1106	40-41	0.30136134913662005
1106	42-43	0.3149332238464666
1106	44-45	0.31223201112865695
1106	46-47	0.25705855199600336
1106	48-49	0.2510431549307839
1106	50-51	0.2519552902857498
1106	52-53	0.21377743593029663
1106	54-55	0.2105150784368064
1106	56-57	0.16696527334428168
1106	58-59	0.21874172548971416
1106	60-61	0.22453717857282385
1106	62-63	0.2082412885260112
1106	64-65	0.2553981792410198
1106	66-67	0.26099035891463984
1106	68-69	0.16976667802072143
1106	70-71	0.16022202466581348
1106	72-73	0.1536054001443503
1106	74-75	0.20954508588347664
1106	76-77	0.1927280336414796
1106	78-79	0.253755874416008
1106	80-81	0.229737961039433
1106	82-83	0.19599140508449153
1106	84-85	0.1628842571603002
1106	86-87	0.2033062337421967
1106	88-89	0.1462759261047495
1106	90-91	0.19643193272855797
1106	92-93	0.17688515612045563
1106	94-95	0.19174437882239204
1106	96-97	0.23830956329194208
1106	98-99	0.3089119823491018
1106	100	0.3180046657908484
1107	1	0.020990664534163983
1107	2	0.06512615717301173
1107	3	0.030419141497723956
1107	4	0.04519751850162379
1107	5	0.014885611660375275
1107	6	0.058732629265229264
1107	7	0.1044602269483832
1107	8	0.11646431304302496
1107	9	0.19692278697998233
1107	10-11	0.26247933264775014
1107	12-13	0.2331726658617157
1107	14-15	0.2870877615899943
1107	16-17	0.30428610107438914
1107	18-19	0.2508656248789052
1107	20-21	0.30784516845205445
1107	22-23	0.2998927253241561
1107	24-25	0.32680498092882715
1107	26-27	0.28454302531105924
1107	28-29	0.27604147350529473
1107	30-31	0.3164707365244084
1107	32-33	0.3114907242310778
1107	34-35	0.29317631899881036
1107	36-37	0.2508050783463176
1107	38-39	0.2550475192798771
1107	40-41	0.20319908397012654
1107	42-43	0.24413715138670966
1107	44-45	0.184945169965971
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2216	58-59	-0.48588811989557
2216	60-61	-0.4547399439641282
2216	62-63	-0.49838047558422005
2216	64-65	-0.4901694739427924
2216	66-67	-0.4416501533082027
2216	68-69	-0.4347528824934557
2216	70-71	-0.40978537210042276
2216	72-73	-0.3661840906262981
2216	74-75	-0.35801192460384357
2216	76-77	-0.3998326213230641
2216	78-79	-0.34490622112245717
2216	80-81	-0.41821164948843403
2216	82-83	-0.3709192970758224
2216	84-85	-0.3529251064769383
2216	86-87	-0.3980200199140711
2216	88-89	-0.39255230958750076
2216	90-91	-0.4027686593529083
2216	92-93	-0.3369911326281496
2216	94-95	-0.3758252577141832
2216	96-97	-0.38564774176020933
2216	98-99	-0.42822264955018596
2216	100	-0.525150202188275
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1507.0
1	390.0
2	270.0
3	237.0
4	211.0
5	243.0
6	407.0
7	1039.0
8	3030.0
9	4043.0
10	4325.0
11	5211.0
12	6903.0
13	8741.0
14	12226.0
15	14845.0
16	15903.0
17	17340.0
18	19001.0
19	21760.0
20	24748.0
21	27436.0
22	30348.0
23	35064.0
24	41582.0
25	49570.0
26	51991.0
27	47367.0
28	50947.0
29	63010.0
30	71703.0
31	81178.0
32	103234.0
33	138431.0
34	204001.0
35	316371.0
36	581500.0
37	1205326.0
38	1431838.0
39	432225.0
40	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.020917505146485	19.822283807728763	13.147088815526109	24.009709871598645
2	15.273569432715735	20.88238405952419	39.818375179932374	24.025671327827705
3	18.156243355696912	26.333746528854995	30.321216727721854	25.18879338772624
4	12.305600467654209	17.939709078938712	38.996839546302006	30.757850907105077
5	13.269620473867322	38.738363548715675	34.9277948911393	13.0642210862777
6	30.075380146933195	38.46125308144786	18.210031485684997	13.253335285933954
7	26.315954924724196	33.63246710088282	22.617867269503115	17.433710704889872
8	25.55371538070727	35.38586592115504	20.668578348427236	18.391840349710456
9	26.243331105162383	16.07726859559634	20.35719194235076	37.32220835689051
10-11	24.18669924828765	26.862158344959862	28.5857354664455	20.365406940306986
12-13	24.955390281958014	24.883696528001803	27.826566513404387	22.334346676635793
14-15	22.490368267170556	26.116704573397726	25.38996718372691	26.002959975704808
16-17	22.239246031486488	28.775184811373645	26.31169046946633	22.673878687673536
18-19	22.324056893915824	28.434316164929214	27.417929434058742	21.823697507096217
20-21	23.32201926661304	27.54450588392964	26.871186651451097	22.26228819800622
22-23	23.30971603899094	27.33107263540638	26.648948896506546	22.710262429096137
24-25	22.994678715957136	27.593159565113496	26.8629667837547	22.549194935174672
26-27	22.824859926184825	27.701823085070572	26.58445371724538	22.888863271499226
28-29	22.73101243024376	28.00795518419475	26.6026974529002	22.658334932661298
30-31	23.024379891056153	27.67394006819192	26.482075068126516	22.819604972625413
32-33	22.847797652670067	27.93789656417298	26.12696613922586	23.087339643931085
34-35	23.273355244724385	27.833656185633338	26.423688826225412	22.46929974341687
36-37	23.29150179222247	27.479218302810814	26.495594908908863	22.73368499605785
38-39	22.997521922487515	27.569571516059572	26.585691857900123	22.84721470355279
40-41	23.07062710214236	27.36900638351316	26.778432084053183	22.781934430291294
42-43	22.896874222934393	27.49248476573098	26.495751528796497	23.114889482538132
44-45	22.781613697392835	27.788098953904594	26.663805591085783	22.766481757616784
46-47	22.943339089789987	27.777659546427664	26.20674237594901	23.072258987833344
48-49	22.893824606170025	27.588893776962102	26.39384774478556	23.123433872082312
50-51	22.880139803133368	27.67362021287761	26.326999724201222	23.11924025978781
52-53	22.781846903667486	27.72887124552353	26.42547088389095	23.063810966918037
54-55	22.59477911183944	27.69729649940771	26.451040188659093	23.256884200093758
56-57	22.922148280305322	27.610823216898407	26.329758515779332	23.137269987016936
58-59	22.74069676844878	27.414739497800266	26.556705922785667	23.28785781096529
60-61	22.56242875728293	27.664976775179344	26.57196765367069	23.200626813867036
62-63	22.77563483515712	27.503247121916917	26.50204102714011	23.21907701578585
64-65	22.434019728444635	27.56564342206665	26.504899500222763	23.495437349265956
66-67	22.660783683088287	27.513805756872216	26.331506886230876	23.493903673808617
68-69	22.716281106050626	27.432150382455784	26.45909788608761	23.392470625405984
70-71	22.65461028639273	27.308052818427868	26.405474385562027	23.631862509617374
72-73	22.766935402061808	27.424933136721336	26.518660256435915	23.28947120478094
74-75	22.781889476243386	27.797082147829773	26.349413871416882	23.071614504509956
76-77	22.846248320533704	27.46391777540001	26.40917242208209	23.2806614819842
78-79	22.776452699928438	27.50938428193924	26.419134942180833	23.295028075951485
80-81	22.822334862814394	27.6008321960027	26.4597750126003	23.1170579285826
82-83	22.998752714218455	27.313937749757404	26.69998099876209	22.98732853726205
84-85	23.030322143914507	27.52586486599167	26.33357316773466	23.110239822359162
86-87	22.796087957206502	27.666389673150583	26.41400210522883	23.123520264414086
88-89	23.003182410870284	27.248027408080368	26.35564405620453	23.393146124844822
90-91	22.929538053122403	27.2542457939924	26.521990647512567	23.29422550537263
92-93	23.12320421761734	27.242986502648215	26.448639281442972	23.185169998291475
94-95	22.629928904164235	27.133690280210654	26.71708520155731	23.519295614067804
96-97	22.88380400047135	27.003332928827195	26.627210558527953	23.485652512173502
98-99	22.744348770570596	27.371858419677903	26.33383914519	23.5499536645615
100	22.990914938108663	27.165580762830743	26.27193702077164	23.571567278288956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5330.0
1	3751.0
2	2372.5
3	2524.5
4	2317.5
5	2055.0
6	1860.0
7	1780.0
8	1808.5
9	1805.5
10	1582.5
11	1367.0
12	1355.5
13	1366.5
14	1407.0
15	1478.5
16	1673.5
17	1928.0
18	2269.0
19	2876.5
20	3795.5
21	5296.5
22	7491.0
23	9815.5
24	12773.0
25	16784.5
26	22312.0
27	29663.0
28	37336.0
29	45926.5
30	55662.0
31	66718.0
32	81478.0
33	91863.0
34	97841.5
35	106191.0
36	117046.0
37	130131.5
38	137446.0
39	138576.0
40	140617.0
41	144070.0
42	149255.0
43	158718.5
44	168321.0
45	182615.5
46	194792.5
47	202752.0
48	213027.5
49	211914.5
50	204893.5
51	204092.5
52	201231.5
53	196283.0
54	191913.0
55	178603.0
56	161944.5
57	144207.0
58	126263.0
59	109932.0
60	89601.5
61	71795.5
62	58954.0
63	44855.0
64	31319.5
65	22602.0
66	16900.0
67	12456.0
68	9074.5
69	6612.5
70	5064.0
71	3920.5
72	2984.5
73	2479.0
74	2074.0
75	1628.5
76	1173.5
77	785.5
78	541.5
79	380.0
80	265.5
81	169.0
82	122.5
83	95.0
84	63.5
85	50.0
86	46.5
87	35.5
88	24.5
89	15.0
90	18.0
91	17.0
92	10.5
93	14.0
94	12.5
95	11.5
96	10.5
97	7.0
98	8.0
99	9.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09914054782078072
2	0.0643379627534307
3	0.08168073076064716
4	0.08433384262676802
5	0.08441187532871276
6	0.09131776945082146
7	0.08759170793296055
8	0.0889962965679657
9	0.0735068052319366
10-11	0.08095892826765841
12-13	0.06971246509987405
14-15	0.07909589750872796
16-17	0.0808516333024844
18-19	0.08071507607408113
20-21	0.07162426629751997
22-23	0.07591606490448016
24-25	0.07772057113695206
26-27	0.07154623359557523
28-29	0.08023712577466964
30-31	0.08456794073260222
32-33	0.08597252936760738
34-35	0.08815744502205984
36-37	0.08743564252907109
38-39	0.08867441167244368
40-41	0.08479228475069332
42-43	0.0902350657113383
44-45	0.08659679098316522
46-47	0.08331941750148653
48-49	0.09260530903290952
50-51	0.08912309970862589
52-53	0.09434153665117978
54-55	0.08655777463219286
56-57	0.09303448889360554
58-59	0.09726776297410719
60-61	0.09595096112878986
62-63	0.09719948435990555
64-65	0.09899423650463436
66-67	0.10114988989585755
68-69	0.09766768057157395
70-71	0.09980382578731095
72-73	0.09698489442955753
74-75	0.10077923456162008
76-77	0.09772620509803248
78-79	0.10519783630924047
80-81	0.1004085792273826
82-83	0.10411513256975734
84-85	0.10069144777193227
86-87	0.09749210699219829
88-89	0.0963411246385135
90-91	0.09673128814823716
92-93	0.09131776945082146
94-95	0.09088858959012544
96-97	0.08943523051640481
98-99	0.1023301345127716
100	0.2437936690508258
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5126056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.33331782489584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.25162627538	35.71834049935474
2	15.465124282689482	16.18682528575006
3	6.545372763295668	10.276232193119068
4	3.2998348365458923	6.907652210824776
5	1.978523965702414	5.177136176063887
6	1.1987066998109328	3.7639379220022526
7	0.7918629152972916	2.9008569534001216
8	0.5439977603921582	2.277536615250748
9	0.3939214573946183	1.8553695145090854
>10	1.4806419608687607	12.350280331043617
>50	0.038429721764276066	1.3471534527801647
>100	0.011613847967291788	0.9494981004031214
>500	2.7481031295263045E-4	0.08312643089578112
>1k	0.0	0.0
>5k	6.870257822235187E-5	0.2060543146025712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5731	0.11180135371131335	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9508175486182748E-5	3.9016350972365497E-5
2	0.0	0.0	0.0	1.9508175486182748E-5	3.9016350972365497E-5
3	0.0	0.0	0.0	1.9508175486182748E-5	3.9016350972365497E-5
4	0.0	0.0	0.0	1.9508175486182748E-5	5.852452645854825E-5
5	0.0	0.0	0.0	1.9508175486182748E-5	5.852452645854825E-5
6	0.0	0.0	0.0	1.9508175486182748E-5	1.950817548618275E-4
7	0.0	0.0	0.0	1.9508175486182748E-5	2.5360628132037576E-4
8	0.0	0.0	0.0	1.9508175486182748E-5	2.5360628132037576E-4
9	0.0	0.0	0.0	5.852452645854825E-5	2.5360628132037576E-4
10-11	0.0	0.0	0.0	5.852452645854825E-5	2.5360628132037576E-4
12-13	0.0	0.0	0.0	5.852452645854825E-5	5.267207381269342E-4
14-15	0.0	0.0	9.754087743091374E-6	5.852452645854825E-5	7.705729317042185E-4
16-17	0.0	0.0	1.9508175486182748E-5	7.803270194473099E-5	9.85162862052229E-4
18-19	0.0	0.0	1.9508175486182748E-5	9.754087743091375E-5	0.0010729496517400512
20-21	0.0	0.0	1.9508175486182748E-5	1.6581949163255337E-4	0.0012680314066018787
22-23	0.0	0.0	1.9508175486182748E-5	5.169666503838429E-4	0.0014143427227482493
24-25	0.0	0.0	1.9508175486182748E-5	0.001297293669831153	0.0015411458634084373
26-27	0.0	0.0	1.9508175486182748E-5	0.0026140955151484884	0.0016191785653531681
28-29	0.0	0.0	1.9508175486182748E-5	0.00793982742287638	0.0016484408285824424
30-31	0.0	0.0	1.9508175486182748E-5	0.020561616962436618	0.001716719442784082
32-33	9.754087743091374E-6	0.0	1.9508175486182748E-5	0.0471610142378468	0.0019703257241044576
34-35	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.09133727762630764	0.002028850250563006
36-37	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.1584356472110332	0.002028850250563006
38-39	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.25563513157093876	0.002077620689278463
40-41	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.3686850085133678	0.002184915654452468
42-43	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.48142275464801787	0.002262948356397199
44-45	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.6185457201403964	0.002399505584800478
46-47	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.7871743890429601	0.0025653250764330316
48-49	1.9508175486182748E-5	0.0	1.9508175486182748E-5	0.9726483674778427	0.002779915006781042
50-51	1.9508175486182748E-5	0.0	1.9508175486182748E-5	1.1691834814133908	0.0028481936209826812
52-53	1.9508175486182748E-5	0.0	1.9508175486182748E-5	1.3598271263521116	0.0031115539900461488
54-55	1.9508175486182748E-5	0.0	1.9508175486182748E-5	1.5670137041031156	0.0034041766223388897
56-57	1.9508175486182748E-5	0.0	1.9508175486182748E-5	1.8159477773945505	0.003569996113971443
58-59	1.9508175486182748E-5	0.0	1.9508175486182748E-5	2.097089848413673	0.003638274728173083
60-61	1.9508175486182748E-5	0.0	1.9508175486182748E-5	2.398930093623636	0.0037650778688332704
62-63	1.9508175486182748E-5	0.0	1.9508175486182748E-5	2.7018725507485675	0.004038192325639829
64-65	1.9508175486182748E-5	0.0	1.9508175486182748E-5	3.013632703193254	0.004057700501126012
66-67	1.9508175486182748E-5	0.0	1.9508175486182748E-5	3.36909116872699	0.004086962764355286
68-69	1.9508175486182748E-5	0.0	1.9508175486182748E-5	3.7654680323429943	0.0041357332030707425
70-71	1.9508175486182748E-5	0.0	1.9508175486182748E-5	4.200617394737787	0.004350323133418753
72-73	1.9508175486182748E-5	0.0	1.9508175486182748E-5	4.651148953503434	0.004838027520573322
74-75	1.9508175486182748E-5	0.0	1.9508175486182748E-5	5.092141014456338	0.004886797959288779
76-77	1.9508175486182748E-5	0.0	1.9508175486182748E-5	5.575719422495579	0.004935568398004235
78-79	1.9508175486182748E-5	0.0	1.9508175486182748E-5	6.116505555148051	0.005023355187692058
80-81	1.9508175486182748E-5	0.0	1.9508175486182748E-5	6.699078199691927	0.005228191030296977
82-83	1.9508175486182748E-5	0.0	1.9508175486182748E-5	7.298944841804303	0.005559830013562084
84-85	1.9508175486182748E-5	0.0	1.9508175486182748E-5	7.8958267330672935	0.005569584101305175
86-87	1.9508175486182748E-5	0.0	1.9508175486182748E-5	8.513914011083765	0.00574515768068082
88	1.9508175486182748E-5	0.0	1.9508175486182748E-5	8.989113657751691	0.005989009874258104
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7955	0.0	33.757256	1
GTATCAA	12015	0.0	30.257072	1
CAACGCA	13525	0.0	25.721228	5
ATCAACG	13635	0.0	25.549198	3
GTACATG	16900	0.0	24.906204	1
GTACTAG	1820	0.0	24.806816	1
TATCAAC	14520	0.0	24.704035	2
AACGCAG	14490	0.0	24.55356	6
TCAACGC	14405	0.0	24.183266	4
TACATGG	16980	0.0	23.755247	2
ACATGGG	17695	0.0	21.97198	3
ACGCAGA	15905	0.0	21.77793	7
CGCAGAG	16115	0.0	21.347895	8
CATGGGG	12410	0.0	19.736761	4
TCTAACG	835	0.0	19.705687	2
TAACGCC	895	0.0	19.435183	4
CTAACGC	850	0.0	18.805037	3
TGATCGC	1160	0.0	18.230879	9
GCAGAGT	17995	0.0	18.124277	9
GTATAAG	2745	0.0	17.818132	1
>>END_MODULE