FastQCFastQC Report
Mon 16 Jan 2017
ERR1146514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5126056
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259380.5060030557606081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237050.4624412998999621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139590.272314621611625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142500.053.0962871
GTATCAA255950.041.834141
CAACGCA325450.032.4787525
ATCAACG328200.032.193363
TATCAAC331450.032.1481822
TCAACGC336700.031.4364994
AACGCAG340100.031.2037836
ACGCAGA374650.028.187377
CGCAGAG377800.027.8403938
GCAGAGT408750.025.3759219
GTACATG164100.024.8446871
TGATCGC10250.023.842779
TACATGG166550.023.7446562
GAGTACT277250.023.47780812-13
ACATGGG168750.022.8492453
AGAGTAC374150.022.3338310-11
CAGAGTA396700.021.745510-11
AGTACTT289400.021.6963812-13
GTACTTT300050.021.47461114-15
GTACTAG18050.021.1023481
Produced by FastQC (version 0.11.5)