##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146514_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5126056
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34611131833129	33.0	31.0	34.0	30.0	34.0
2	32.450538971872334	34.0	31.0	34.0	30.0	34.0
3	32.54736019270956	34.0	31.0	34.0	31.0	34.0
4	36.051591125809004	37.0	35.0	37.0	35.0	37.0
5	36.09314549041212	37.0	35.0	37.0	35.0	37.0
6	36.15387600135465	37.0	36.0	37.0	35.0	37.0
7	36.11771993907207	37.0	36.0	37.0	35.0	37.0
8	36.122855661350556	37.0	36.0	37.0	35.0	37.0
9	37.906882991524085	39.0	38.0	39.0	35.0	39.0
10-11	37.85682091650969	39.0	38.0	39.0	35.0	39.0
12-13	37.82357010926139	39.0	38.0	39.0	35.0	39.0
14-15	39.16431999572381	40.0	39.0	41.0	36.0	41.0
16-17	39.11499328138436	40.0	39.0	41.0	36.0	41.0
18-19	39.235918511229684	40.5	39.0	41.0	36.0	41.0
20-21	39.29278893948876	41.0	39.0	41.0	36.0	41.0
22-23	39.255325926989485	41.0	39.0	41.0	36.0	41.0
24-25	39.23740405879296	41.0	39.0	41.0	36.0	41.0
26-27	39.148077683895764	41.0	39.0	41.0	35.5	41.0
28-29	39.07749934842694	40.5	39.0	41.0	35.0	41.0
30-31	38.90456561535808	40.0	38.5	41.0	35.0	41.0
32-33	38.75713238403951	40.0	38.0	41.0	35.0	41.0
34-35	38.66466597321606	40.0	38.0	41.0	35.0	41.0
36-37	38.57742404686957	40.0	38.0	41.0	35.0	41.0
38-39	38.47649557476547	40.0	38.0	41.0	34.5	41.0
40-41	38.36275725431014	40.0	38.0	41.0	34.0	41.0
42-43	38.237866012388466	40.0	38.0	41.0	34.0	41.0
44-45	38.063166984519874	40.0	38.0	41.0	33.5	41.0
46-47	37.77530190852383	40.0	37.5	41.0	33.0	41.0
48-49	37.44804719261748	40.0	37.0	41.0	32.0	41.0
50-51	37.31907035350375	40.0	37.0	41.0	32.0	41.0
52-53	37.20780430412778	40.0	36.5	41.0	32.0	41.0
54-55	37.04260214870848	40.0	36.0	41.0	31.0	41.0
56-57	36.8807269175366	39.5	35.5	41.0	31.0	41.0
58-59	36.69483136742946	39.0	35.0	41.0	31.0	41.0
60-61	36.48748150624964	39.0	35.0	41.0	31.0	41.0
62-63	36.24679753791219	38.5	35.0	41.0	31.0	41.0
64-65	35.968676405407976	37.5	35.0	40.0	30.0	41.0
66-67	35.69024928717127	37.0	35.0	40.0	30.0	41.0
68-69	35.385714377681396	36.5	35.0	39.5	30.0	41.0
70-71	35.06165363780653	36.0	35.0	39.0	29.5	41.0
72-73	34.7345358692921	35.5	35.0	39.0	29.0	41.0
74-75	34.42446551500802	35.0	34.0	38.0	29.0	40.0
76-77	34.133324236020826	35.0	34.0	37.0	29.0	39.0
78-79	33.85770970898484	35.0	34.0	37.0	29.0	39.0
80-81	33.606911922148335	35.0	34.0	36.0	29.0	39.0
82-83	33.36769594401622	35.0	34.0	36.0	29.0	37.0
84-85	33.167403262859395	35.0	34.0	35.5	29.0	37.0
86-87	32.9901379345056	35.0	34.0	35.0	28.5	37.0
88-89	32.80326726824678	35.0	34.0	35.0	27.0	36.0
90-91	32.727471958948556	35.0	34.0	35.0	27.0	36.0
92-93	32.6395262751714	35.0	34.0	35.0	27.0	36.0
94-95	32.572637033227885	35.0	34.0	35.0	27.0	36.0
96-97	32.5198460570856	35.0	34.0	35.0	27.0	35.0
98-99	32.448554210098365	35.0	34.0	35.0	27.0	35.0
100	31.724166103530667	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3275641918025727
1101	2	0.34991299786034347
1101	3	0.34068568104729735
1101	4	0.23732228443951442
1101	5	0.24195493800732493
1101	6	0.22539779025852624
1101	7	0.22619379889485458
1101	8	0.2579747567141766
1101	9	0.2974013149279102
1101	10-11	0.3214072763998388
1101	12-13	0.3368860097301507
1101	14-15	0.4845604763237077
1101	16-17	0.5164232616135962
1101	18-19	0.4947323506515531
1101	20-21	0.41392933522569564
1101	22-23	0.4341658758640108
1101	24-25	0.4440503331461372
1101	26-27	0.434575644434652
1101	28-29	0.4718219493548901
1101	30-31	0.5297303722074247
1101	32-33	0.48037271376886537
1101	34-35	0.5246778377328312
1101	36-37	0.49668444943854695
1101	38-39	0.48200666236125755
1101	40-41	0.49381570628813876
1101	42-43	0.5096508021925672
1101	44-45	0.46635587673150525
1101	46-47	0.5379666549414779
1101	48-49	0.6360687973451107
1101	50-51	0.5587087584178789
1101	52-53	0.5150557406604008
1101	54-55	0.521355828390881
1101	56-57	0.4933493713896908
1101	58-59	0.4634904005202287
1101	60-61	0.4726296156690921
1101	62-63	0.481844698305693
1101	64-65	0.43371555628825575
1101	66-67	0.38160858996782565
1101	68-69	0.43379976424104427
1101	70-71	0.39501064846015055
1101	72-73	0.35837481046193176
1101	74-75	0.3611147223992077
1101	76-77	0.3408132886241475
1101	78-79	0.34484535073043787
1101	80-81	0.27001342459668365
1101	82-83	0.2691840974247057
1101	84-85	0.2862444522257732
1101	86-87	0.26699332004006493
1101	88-89	0.3687298389155913
1101	90-91	0.3383607494265348
1101	92-93	0.3755071206203624
1101	94-95	0.4346023539714423
1101	96-97	0.34830823900027497
1101	98-99	0.3800073179472463
1101	100	0.4726883173869254
1102	1	0.26899411849123567
1102	2	0.2837864135725283
1102	3	0.292536883971664
1102	4	0.1611139877019454
1102	5	0.18910278939593894
1102	6	0.1977924150424144
1102	7	0.20690343105030706
1102	8	0.22664054433368364
1102	9	0.22245206230518733
1102	10-11	0.24960806754227605
1102	12-13	0.26473282340884197
1102	14-15	0.3819992968131487
1102	16-17	0.4513580481451527
1102	18-19	0.3684237946175415
1102	20-21	0.3201505780665954
1102	22-23	0.36725477568482745
1102	24-25	0.35497006056664304
1102	26-27	0.3136327203524374
1102	28-29	0.3444396679205255
1102	30-31	0.34579331168420424
1102	32-33	0.3854934629506559
1102	34-35	0.3677473296300988
1102	36-37	0.37366689170708867
1102	38-39	0.3646343993689172
1102	40-41	0.36266490327781753
1102	42-43	0.375254113819274
1102	44-45	0.36710740963549426
1102	46-47	0.31681535026029195
1102	48-49	0.41050072996846865
1102	50-51	0.36748698769943644
1102	52-53	0.3238852601720268
1102	54-55	0.3114374584767603
1102	56-57	0.29145417866946133
1102	58-59	0.29152586490646115
1102	60-61	0.2828039125998387
1102	62-63	0.30348666704186655
1102	64-65	0.34526705205362873
1102	66-67	0.2928375562937262
1102	68-69	0.20635174146069346
1102	70-71	0.23255726828423207
1102	72-73	0.19634148603886814
1102	74-75	0.16943040365701023
1102	76-77	0.15491222410852146
1102	78-79	0.19168411296615773
1102	80-81	0.14871277923829496
1102	82-83	0.14699073999619117
1102	84-85	0.1999424628387345
1102	86-87	0.23698497882600833
1102	88-89	0.2648186406485209
1102	90-91	0.24046042918082122
1102	92-93	0.2479610473928915
1102	94-95	0.24337197248443942
1102	96-97	0.23699870583122618
1102	98-99	0.2540280302150464
1102	100	0.27194284945904457
1103	1	0.1977690935779961
1103	2	0.24160422530614056
1103	3	0.1998080178247008
1103	4	0.16698503250338348
1103	5	0.129623387879235
1103	6	0.16060701102378516
1103	7	0.14128653668954172
1103	8	0.15489251795744963
1103	9	0.2104958248059816
1103	10-11	0.2189732568476046
1103	12-13	0.23109782188930694
1103	14-15	0.3626305737141493
1103	16-17	0.4284307948216224
1103	18-19	0.3390766983357949
1103	20-21	0.2536823928109939
1103	22-23	0.27466372749095314
1103	24-25	0.3195420121395358
1103	26-27	0.3066008243307792
1103	28-29	0.3324809567657496
1103	30-31	0.3240724955192178
1103	32-33	0.3634641465983819
1103	34-35	0.3803103195022359
1103	36-37	0.39110757698035314
1103	38-39	0.32619697225341326
1103	40-41	0.34963262560644637
1103	42-43	0.37153302908596686
1103	44-45	0.3945572120964371
1103	46-47	0.4281019338592458
1103	48-49	0.5167527177274067
1103	50-51	0.47374922346001824
1103	52-53	0.43535666117986693
1103	54-55	0.469798767676636
1103	56-57	0.3965279996192024
1103	58-59	0.4359159674571629
1103	60-61	0.3556880536406126
1103	62-63	0.3710531553697507
1103	64-65	0.43336558835629546
1103	66-67	0.3887705131602459
1103	68-69	0.31886384470401197
1103	70-71	0.3138256881837336
1103	72-73	0.3337456318856056
1103	74-75	0.3611327027431699
1103	76-77	0.3735660607483027
1103	78-79	0.3401656418266441
1103	80-81	0.27127075112343846
1103	82-83	0.2722407558982596
1103	84-85	0.2842237952202993
1103	86-87	0.2701104072984961
1103	88-89	0.33323571311249367
1103	90-91	0.31679249270430176
1103	92-93	0.33563376155845503
1103	94-95	0.3201867522230799
1103	96-97	0.275247428364807
1103	98-99	0.3168214234344049
1103	100	0.34743316333059937
1104	1	0.19387102688064317
1104	2	0.193942674073881
1104	3	0.18051278531491732
1104	4	0.1731848228414279
1104	5	0.15872122434371505
1104	6	0.14107194588861205
1104	7	0.1381943656960587
1104	8	0.15281396853745122
1104	9	0.21047275311715907
1104	10-11	0.17452574917485464
1104	12-13	0.2000376414864462
1104	14-15	0.29834899665934955
1104	16-17	0.377968735850871
1104	18-19	0.3270669767650958
1104	20-21	0.2828999842476847
1104	22-23	0.2937662186283134
1104	24-25	0.3024970961595983
1104	26-27	0.3399920721743612
1104	28-29	0.29950203468958136
1104	30-31	0.32408064321352015
1104	32-33	0.32925927887539075
1104	34-35	0.3160340061806366
1104	36-37	0.31613959298210403
1104	38-39	0.3175840930074898
1104	40-41	0.3388324131210325
1104	42-43	0.3499551514473893
1104	44-45	0.32328521584275904
1104	46-47	0.3430963938304785
1104	48-49	0.4356793074050813
1104	50-51	0.3815713326721948
1104	52-53	0.3018710896574248
1104	54-55	0.32855160979004694
1104	56-57	0.3469067262313885
1104	58-59	0.28514778509078553
1104	60-61	0.24338810071169803
1104	62-63	0.26344744407804654
1104	64-65	0.23511540654573793
1104	66-67	0.23356829101779653
1104	68-69	0.2337136629337948
1104	70-71	0.2807337471004345
1104	72-73	0.2309043655080245
1104	74-75	0.2288223756213128
1104	76-77	0.1946260757771725
1104	78-79	0.20133419463076052
1104	80-81	0.21533071896021738
1104	82-83	0.17618428027621036
1104	84-85	0.20264936220100083
1104	86-87	0.22408055285583828
1104	88-89	0.204498069272411
1104	90-91	0.17681482222690903
1104	92-93	0.21133630860029484
1104	94-95	0.22072936042910385
1104	96-97	0.2058708600849144
1104	98-99	0.21666597188071535
1104	100	0.22868826320237545
1105	1	0.14240581628356352
1105	2	0.16450877917902318
1105	3	0.14929567178435832
1105	4	0.11283730529853386
1105	5	0.11791677960484748
1105	6	0.13457016384040088
1105	7	0.12677754509915928
1105	8	0.11102312966274752
1105	9	0.1483189205762443
1105	10-11	0.15561064773540068
1105	12-13	0.1578110343366177
1105	14-15	0.27613571907563994
1105	16-17	0.3420126935342722
1105	18-19	0.2698315770017885
1105	20-21	0.20105026057303377
1105	22-23	0.24238473234462532
1105	24-25	0.24704573432863697
1105	26-27	0.2597443270152553
1105	28-29	0.2633367596177578
1105	30-31	0.2833865694357769
1105	32-33	0.2470192383818457
1105	34-35	0.21564260476906583
1105	36-37	0.23496450156615367
1105	38-39	0.24458269497531404
1105	40-41	0.26691170636610195
1105	42-43	0.26202630525348525
1105	44-45	0.27813914266521067
1105	46-47	0.3406862181974688
1105	48-49	0.39840952293237564
1105	50-51	0.35209729224216346
1105	52-53	0.302364228204155
1105	54-55	0.3331240653024352
1105	56-57	0.3022148555032871
1105	58-59	0.2603453545731895
1105	60-61	0.24573257655730885
1105	62-63	0.2725591735890589
1105	64-65	0.2761094599346947
1105	66-67	0.25165133820203067
1105	68-69	0.19253491667456046
1105	70-71	0.20881077752623156
1105	72-73	0.19729432058794316
1105	74-75	0.20172817734169968
1105	76-77	0.17495932819520732
1105	78-79	0.18422819769930499
1105	80-81	0.19219703963401003
1105	82-83	0.26373935164046003
1105	84-85	0.16282068024875684
1105	86-87	0.18233854015924322
1105	88-89	0.2854378274831504
1105	90-91	0.20786602818863997
1105	92-93	0.23001432119889387
1105	94-95	0.2791740420274067
1105	96-97	0.26974603485844284
1105	98-99	0.24659889645525368
1105	100	0.18546041704193783
1106	1	0.13296516457424445
1106	2	0.11282506030671868
1106	3	0.14425837373113382
1106	4	0.09797429120669676
1106	5	0.1434426705423988
1106	6	0.11217499129575259
1106	7	0.13823066212408008
1106	8	0.10772816093109583
1106	9	0.13698058180060713
1106	10-11	0.14001039257423287
1106	12-13	0.17411917937864985
1106	14-15	0.24979745027758327
1106	16-17	0.30356271169280546
1106	18-19	0.2305471828026242
1106	20-21	0.23961572419180044
1106	22-23	0.1688754547238105
1106	24-25	0.18410472310712578
1106	26-27	0.19825487229974215
1106	28-29	0.19146062377178907
1106	30-31	0.22808646333625404
1106	32-33	0.24784635280901313
1106	34-35	0.18808923991841908
1106	36-37	0.20746274753012983
1106	38-39	0.2316802821143824
1106	40-41	0.2429287367717592
1106	42-43	0.24103376118702613
1106	44-45	0.21554941704825836
1106	46-47	0.221534088389312
1106	48-49	0.3817933358072807
1106	50-51	0.30122831508446524
1106	52-53	0.2704932932124393
1106	54-55	0.29915161711014093
1106	56-57	0.2515382416635106
1106	58-59	0.17683412245776253
1106	60-61	0.22471144111263897
1106	62-63	0.20128509825092777
1106	64-65	0.19064686625618776
1106	66-67	0.15350163937392125
1106	68-69	0.21279340899866384
1106	70-71	0.18881286259563268
1106	72-73	0.1943224119172129
1106	74-75	0.212600105189658
1106	76-77	0.18392643989305668
1106	78-79	0.17620396361711244
1106	80-81	0.22492190754052643
1106	82-83	0.1872432317944046
1106	84-85	0.17923203753963435
1106	86-87	0.21238676540946955
1106	88-89	0.31238594249898455
1106	90-91	0.21511642836475175
1106	92-93	0.19439206230816808
1106	94-95	0.22839275260052716
1106	96-97	0.18540650638880152
1106	98-99	0.3047791805537443
1106	100	0.28893087004756524
1107	1	0.12041301718841879
1107	2	0.07821095091744468
1107	3	0.07529460685410783
1107	4	0.08102155768897035
1107	5	0.06670518915852597
1107	6	0.054982369021040256
1107	7	0.06872359745126744
1107	8	0.07661452504384414
1107	9	0.06232744286036507
1107	10-11	0.0905045706478731
1107	12-13	0.08632343762576511
1107	14-15	0.14357556970118424
1107	16-17	0.23322540116260626
1107	18-19	0.14246208807320215
1107	20-21	0.13860036022850863
1107	22-23	0.14847727677256017
1107	24-25	0.16300790405350085
1107	26-27	0.174880962638035
1107	28-29	0.15060981562540832
1107	30-31	0.19869824299301087
1107	32-33	0.1424656777577269
1107	34-35	0.12383237966782445
1107	36-37	0.10891762167950958
1107	38-39	0.1409023837122234
1107	40-41	0.14843723260856478
1107	42-43	0.20242049122843753
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2216	46-47	-0.5222364685471632
2216	48-49	-0.44028805243321045
2216	50-51	-0.448634945007818
2216	52-53	-0.47600218598982735
2216	54-55	-0.45577037489169925
2216	56-57	-0.43757796122411463
2216	58-59	-0.5101287115008972
2216	60-61	-0.4758138958087912
2216	62-63	-0.4986745971503339
2216	64-65	-0.4863415808195839
2216	66-67	-0.5067066099229081
2216	68-69	-0.4352021518442726
2216	70-71	-0.3840492489308005
2216	72-73	-0.3626712795297422
2216	74-75	-0.3722783154889555
2216	76-77	-0.38280452795280695
2216	78-79	-0.3918532057622741
2216	80-81	-0.33725030211493134
2216	82-83	-0.39216550341809864
2216	84-85	-0.39863084677507743
2216	86-87	-0.31910352730528047
2216	88-89	-0.2527265043687308
2216	90-91	-0.2791784559578687
2216	92-93	-0.3125025287115335
2216	94-95	-0.28628047920940247
2216	96-97	-0.2908844546821996
2216	98-99	-0.32414317263129533
2216	100	-0.3533216078128909
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	29.0
8	164.0
9	586.0
10	800.0
11	474.0
12	459.0
13	567.0
14	897.0
15	1719.0
16	3047.0
17	5367.0
18	9028.0
19	13512.0
20	19002.0
21	28183.0
22	40155.0
23	46578.0
24	43734.0
25	38281.0
26	34365.0
27	37018.0
28	44343.0
29	52876.0
30	64221.0
31	80253.0
32	102223.0
33	132549.0
34	189946.0
35	299375.0
36	532107.0
37	1200460.0
38	1586531.0
39	516172.0
40	1034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18185897265822	19.361567310348487	13.708132459137154	23.748441257856136
2	15.538611006521819	20.852167534486828	39.57636459761952	24.032856861371826
3	17.838246350693186	26.376256768867677	31.324538503586197	24.46095837685294
4	12.219356312012717	17.901743846181624	38.597506170164806	31.28139367164086
5	12.585560217264327	38.24586093974899	35.137932258820946	14.030646584165737
6	29.49025243318496	38.69876108901805	17.633626179134854	14.177360298662139
7	25.382711386687934	34.07143815830338	22.75298982297501	17.792860632033673
8	25.914426157167664	34.438334234940925	20.485623471892183	19.16161613599923
9	26.517336727236973	14.549632134191329	20.65672737743301	38.276303761138685
10-11	24.391483386384238	26.08412147244326	28.500445225725883	21.023949915446615
12-13	25.791378400860232	24.266921781580226	28.288122486371588	21.653577331187954
14-15	22.819324297078776	24.980108970749335	26.960501492912336	25.24006523925955
16-17	21.40197959925186	27.819129298215238	27.77271918123892	23.006171921293983
18-19	21.37200317203614	26.299895923660443	30.75623604552828	21.57186485877514
20-21	22.329438353271556	25.37771584999539	30.71804418320761	21.574801613525445
22-23	22.23296038903984	25.276294289410806	30.561224848109347	21.929520473440007
24-25	21.98745000054623	25.529120243711734	30.749907921411708	21.73352183433033
26-27	21.855274749885094	25.683694872693252	30.475401693781794	21.98562868363986
28-29	21.738715249836375	25.87822409240634	30.53774296218047	21.845317695576814
30-31	22.045403026036052	25.577111481246952	30.403901954840045	21.973583537876955
32-33	21.958235812520087	25.764427431103037	30.001838650381853	22.275498105995027
34-35	22.302890813459918	25.72436643937333	30.16767203821813	21.80507070894862
36-37	22.421908003886422	25.44240905796243	30.15744958572906	21.97823335242209
38-39	22.215760628043544	25.567282882957404	30.139714795940865	22.07724169305819
40-41	22.26434926528898	25.48739970062596	30.156091586684354	22.092159447400707
42-43	22.306720800553094	25.523199512451676	29.75628826528622	22.41379142170901
44-45	22.273741482796847	25.896267525162052	29.61150677742831	22.21848421461279
46-47	22.610560633750392	25.951930685111517	28.797832095474575	22.63967658566352
48-49	22.828357964374923	26.04558049742765	28.462069834640776	22.663991703556654
50-51	22.672245484637703	26.312861193869125	28.260352598567007	22.754540722926166
52-53	22.565999816232303	26.468088310716446	28.266934085727435	22.698977787323813
54-55	22.40269408875764	26.445231818924462	28.159031220509018	22.99304287180888
56-57	22.702223749931356	26.335802324148904	28.013771231388073	22.948202694531666
58-59	22.3864260963078	26.214606382641474	28.330042113631208	23.06892540741951
60-61	22.303750584273047	26.39024796587143	28.312340471023568	22.993660978831954
62-63	22.373533360170136	26.267197589252767	28.29616904492947	23.063100005647627
64-65	22.01464679815881	26.26241334676802	28.360887118406332	23.36205273666684
66-67	22.187503779751765	26.23764191870539	28.184931012458453	23.38992328908439
68-69	22.267765119603023	26.11341361719951	28.24545329424194	23.373367968955527
70-71	22.20760578459771	25.937681431978227	28.16291538168606	23.691797401738
72-73	22.3171538940703	26.026778860100713	28.187234693397794	23.468832552431188
74-75	22.32352087386255	26.392390691097734	27.876140377983944	23.407948057055773
76-77	22.341404507150514	26.10427918854513	27.828567136604526	23.725749167699828
78-79	22.20662956878862	26.174400084120343	27.84012725738123	23.7788430897098
80-81	22.247574427341675	26.255724242226798	27.76289181089527	23.733809519536262
82-83	22.414314950247515	25.954094453880494	28.030995944128644	23.600594651743346
84-85	22.432791120776805	26.113931355117813	27.59780758379675	23.85546994030863
86-87	22.079918752350736	26.28411589729024	27.650263672499868	23.985701677859154
88-89	22.234091863218037	25.918786294960494	27.552654516454755	24.294467325366714
90-91	22.106842190842453	25.878553207237026	27.716516139944254	24.29808846197627
92-93	22.220767778084166	25.86236209292864	27.630740736802977	24.28612939218422
94-95	21.713873255235512	25.704879975809835	27.87922474419875	24.702022024755905
96-97	21.86720707071988	25.529819211356287	27.80383535754006	24.799138360383775
98-99	21.71597545984025	25.789426607815596	27.550784020394666	24.94381391194949
100	21.854908568888295	25.454168475023593	27.6033067209574	25.087616235130717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1054.0
1	678.0
2	1056.0
3	1735.5
4	1073.5
5	790.5
6	1793.0
7	4609.5
8	8570.0
9	10244.0
10	7259.5
11	3953.0
12	3586.0
13	3861.5
14	4320.5
15	4908.5
16	5659.5
17	6473.5
18	7269.5
19	8320.5
20	9521.0
21	11108.5
22	13105.5
23	15199.0
24	18107.5
25	21665.0
26	26245.0
27	33167.5
28	40654.5
29	48724.0
30	57674.5
31	67700.0
32	81604.5
33	90860.0
34	95361.5
35	103024.0
36	112496.5
37	125212.5
38	133259.0
39	134247.0
40	136650.0
41	139212.5
42	143386.5
43	153093.5
44	163853.0
45	177964.0
46	189777.5
47	198367.0
48	208868.0
49	209146.0
50	202803.5
51	201761.5
52	198807.5
53	194579.0
54	191028.0
55	177200.5
56	160305.5
57	143143.5
58	125359.0
59	109446.0
60	89870.5
61	71533.5
62	57616.5
63	43467.0
64	30288.0
65	21432.0
66	15818.0
67	11675.0
68	8420.0
69	5981.5
70	4559.0
71	3652.5
72	2860.0
73	2344.5
74	1848.0
75	1477.0
76	1108.0
77	677.5
78	406.0
79	246.0
80	169.5
81	99.5
82	50.0
83	25.0
84	18.5
85	11.5
86	8.0
87	8.0
88	4.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0024970464622313918
2	0.0036285206404299913
3	2.731144568065585E-4
4	0.05093584619442316
5	0.0026140955151484884
6	0.005150158328352246
7	0.0
8	5.267207381269343E-4
9	3.9016350972365497E-5
10-11	8.778678968782238E-5
12-13	0.0
14-15	2.9262263229274124E-5
16-17	3.6090124649438085E-4
18-19	2.1458993034801022E-4
20-21	2.6336036906346715E-4
22-23	0.0
24-25	0.0
26-27	2.34098105834193E-4
28-29	1.9508175486182748E-5
30-31	1.950817548618275E-4
32-33	2.6336036906346715E-4
34-35	1.4631131614637063E-4
36-37	4.8770438715456876E-5
38-39	8.193433704196756E-4
40-41	5.267207381269343E-4
42-43	0.0
44-45	6.535238787871221E-4
46-47	0.0
48-49	7.803270194473099E-5
50-51	0.0
52-53	3.7065533423747223E-4
54-55	3.6090124649438085E-4
56-57	1.4631131614637063E-4
58-59	8.486056336489496E-4
60-61	5.46228913613117E-4
62-63	2.048358426049189E-4
64-65	4.096716852098378E-4
66-67	0.0011314741781985995
68-69	0.0023312269705988383
70-71	0.0032773734816787022
72-73	0.0028677017964688642
74-75	0.0024190137602866607
76-77	0.003921143272722733
78-79	0.001297293669831153
80-81	9.754087743091374E-4
82-83	5.949993523285738E-4
84-85	4.2917986069602044E-4
86-87	0.0
88-89	0.0
90-91	9.754087743091374E-6
92-93	8.778678968782238E-5
94-95	1.170490529170965E-4
96-97	2.9262263229274125E-4
98-99	1.8532766711873612E-4
100	0.07809122647118955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5126056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.036636003973264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.88121249541965	32.79629672642282
2	16.18934202802734	15.877417443444058
3	6.816860560222151	10.028277299443646
4	3.444992127751265	6.757233000203685
5	2.0066515484170084	4.919972078326708
6	1.2148546544044494	3.574343129145823
7	0.8144444857331852	2.795633245463898
8	0.5517120741325473	2.1643283326587865
9	0.40881826542604405	1.8042365226125383
>10	1.6043794377124345	12.551935858658608
>50	0.045841761848468755	1.5332447781219676
>100	0.01833399688414113	1.6328764784024417
>500	0.0012072663435020497	0.37682719560122685
>1k	0.0011362506760041492	0.9725213094678334
>5k	0.0	0.0
>10k+	2.13047001750778E-4	2.2148566020257507
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25938	0.5060030557606081	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23705	0.4624412998999621	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13959	0.272314621611625	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9508175486182748E-5	1.5606540388946199E-4
2	0.0	0.0	0.0	1.9508175486182748E-5	1.5606540388946199E-4
3	0.0	0.0	0.0	1.9508175486182748E-5	1.5606540388946199E-4
4	0.0	0.0	0.0	1.9508175486182748E-5	1.5606540388946199E-4
5	0.0	0.0	0.0	1.9508175486182748E-5	1.5606540388946199E-4
6	0.0	0.0	0.0	1.9508175486182748E-5	1.950817548618275E-4
7	0.0	0.0	0.0	1.9508175486182748E-5	1.950817548618275E-4
8	0.0	0.0	0.0	1.9508175486182748E-5	2.1458993034801025E-4
9	0.0	0.0	0.0	3.9016350972365497E-5	2.9262263229274125E-4
10-11	0.0	0.0	0.0	3.9016350972365497E-5	3.0237672003583264E-4
12-13	0.0	0.0	0.0	3.9016350972365497E-5	5.852452645854825E-4
14-15	0.0	0.0	0.0	3.9016350972365497E-5	9.266383355936806E-4
16-17	0.0	0.0	0.0	6.827861420163962E-5	0.0012875395820880614
18-19	0.0	0.0	0.0	1.0729496517400512E-4	0.0013655722840327925
20-21	0.0	0.0	0.0	1.7557357937564473E-4	0.0014338508982344321
22-23	0.0	0.0	0.0	5.169666503838429E-4	0.001589916302123894
24-25	0.0	0.0	0.0	0.001297293669831153	0.0017069653550409905
26-27	0.0	0.0	0.0	0.002653111866120854	0.0017947521447288129
28-29	0.0	0.0	0.0	0.007988597861591836	0.0018142603202149956
30-31	0.0	0.0	0.0	0.02071768236632608	0.001872784846673544
32-33	0.0	0.0	0.0	0.04734634190496553	0.001999587987333732
34-35	0.0	0.0	0.0	0.09170793296054511	0.0021654074789662853
36-37	0.0	0.0	0.0	0.15903064656336177	0.002194669742195559
38-39	9.754087743091374E-6	0.0	0.0	0.2562301309232673	0.002223932005424834
40-41	1.9508175486182748E-5	0.0	0.0	0.3690946801985776	0.0023019647073695645
42-43	1.9508175486182748E-5	0.0	0.0	0.48154955778867803	0.0023214728828557475
44-45	1.9508175486182748E-5	0.0	0.0	0.6187603100707444	0.0024385219357728437
46-47	1.9508175486182748E-5	0.0	0.0	0.787691355693344	0.0027018823048363108
48-49	1.9508175486182748E-5	0.0	0.0	0.9733701699708314	0.002974996761642869
50-51	1.9508175486182748E-5	0.0	0.0	1.1706758568380837	0.003023767200358326
52-53	1.9508175486182748E-5	0.0	0.0	1.3620510583575363	0.0032676193939356105
54-55	1.9508175486182748E-5	0.0	0.0	1.5710909127797277	0.0034627011487974382
56-57	1.9508175486182748E-5	0.0	0.0	1.8220635904094689	0.003511471587512895
58-59	1.9508175486182748E-5	0.0	0.0	2.1052247575914116	0.0036187665526869
60-61	1.9508175486182748E-5	0.0	0.0	2.4089280335603043	0.0036285206404299913
62-63	1.9508175486182748E-5	0.0	0.0	2.7142017176558353	0.0037845860443194534
64-65	1.9508175486182748E-5	0.0	0.0	3.028761293282789	0.0038431105707780015
66-67	1.9508175486182748E-5	0.0	0.0	3.3877019681408083	0.0038918810094934588
68-69	1.9508175486182748E-5	0.0	0.0	3.787297680712033	0.003911389184979641
70-71	1.9508175486182748E-5	0.0	0.0	4.22628039958986	0.004057700501126011
72-73	1.9508175486182748E-5	0.0	0.0	4.681250068278614	0.004652699853454586
74-75	1.9508175486182748E-5	0.0	0.0	5.126192534767471	0.004711224379913134
76-77	1.9508175486182748E-5	0.0	0.0	5.612199710654741	0.004779502994114774
78-79	1.9508175486182748E-5	0.0	0.0	6.155463381593959	0.0048087652573440475
80-81	1.9508175486182748E-5	0.0	0.0	6.739635696527701	0.004984338836719692
82-83	1.9508175486182748E-5	0.0	0.0	7.341014222240256	0.005296469644498617
84-85	1.9508175486182748E-5	0.0	0.0	7.938520375118804	0.0053257319077278905
86-87	1.9508175486182748E-5	0.0	0.0	8.558275602139345	0.005462289136131169
88	1.9508175486182748E-5	0.0	0.0	9.035016394670679	0.005598846364534449
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14250	0.0	53.096287	1
GTATCAA	25595	0.0	41.83414	1
CAACGCA	32545	0.0	32.478752	5
ATCAACG	32820	0.0	32.19336	3
TATCAAC	33145	0.0	32.148182	2
TCAACGC	33670	0.0	31.436499	4
AACGCAG	34010	0.0	31.203783	6
ACGCAGA	37465	0.0	28.18737	7
CGCAGAG	37780	0.0	27.840393	8
GCAGAGT	40875	0.0	25.375921	9
GTACATG	16410	0.0	24.844687	1
TGATCGC	1025	0.0	23.84277	9
TACATGG	16655	0.0	23.744656	2
GAGTACT	27725	0.0	23.477808	12-13
ACATGGG	16875	0.0	22.849245	3
AGAGTAC	37415	0.0	22.33383	10-11
CAGAGTA	39670	0.0	21.7455	10-11
AGTACTT	28940	0.0	21.69638	12-13
GTACTTT	30005	0.0	21.474611	14-15
GTACTAG	1805	0.0	21.102348	1
>>END_MODULE