FastQCFastQC Report
Mon 16 Jan 2017
ERR1146513_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146513_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3343163
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34410.10292648010282479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.038.99481
GTATCAA62350.033.3373261
GTACTAG11250.030.933151
CAACGCA65600.029.5970235
ATCAACG66250.029.2374293
AACGCAG70750.027.6328496
TCAACGC71050.027.4603064
TATCAAC75250.027.2400442
GTACATG104550.025.9985281
TACATGG105550.024.5871772
ACGCAGA79250.024.194677
CGCAGAG79450.023.956318
ACATGGG102500.023.851043
CATGGGG70500.022.5397424
TGATCGC7050.021.3304569
GCAGAGT87150.020.8141739
CGTTAAC2751.4424586E-920.5063239
GTGATCG7150.020.375798
TATTCGC2151.1969369E-619.6717669
CTAGGCA19000.019.5476634
Produced by FastQC (version 0.11.5)