##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146513_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3343163
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208096942925007	31.0	31.0	34.0	28.0	34.0
2	31.222419905939375	31.0	31.0	34.0	28.0	34.0
3	31.418123196505825	33.0	31.0	34.0	28.0	34.0
4	34.85526012342204	37.0	35.0	37.0	32.0	37.0
5	34.898171880940296	37.0	35.0	37.0	32.0	37.0
6	34.90954913056886	37.0	35.0	37.0	32.0	37.0
7	34.872458207990455	37.0	35.0	37.0	32.0	37.0
8	34.80442084337497	37.0	35.0	37.0	32.0	37.0
9	36.36577516561412	39.0	37.0	39.0	32.0	39.0
10-11	36.36827070651356	39.0	37.0	39.0	32.0	39.0
12-13	36.31412422906092	39.0	37.0	39.0	32.0	39.0
14-15	37.65798107959438	40.0	38.0	41.0	31.5	41.0
16-17	37.69713860795899	40.0	38.0	41.0	31.5	41.0
18-19	37.73190164523835	40.0	38.0	41.0	31.0	41.0
20-21	37.85305323132614	40.0	38.0	41.0	32.0	41.0
22-23	37.889158709880434	40.0	38.0	41.0	32.0	41.0
24-25	37.92804119930736	40.0	38.0	41.0	31.5	41.0
26-27	38.00746000120245	40.0	38.0	41.0	32.0	41.0
28-29	37.99693404120589	40.0	38.0	41.0	32.0	41.0
30-31	37.96333950812449	40.0	38.0	41.0	31.0	41.0
32-33	37.93188875325553	40.0	38.0	41.0	31.0	41.0
34-35	37.883121612676376	40.0	38.0	41.0	31.0	41.0
36-37	37.80550095822429	40.0	38.0	41.0	31.0	41.0
38-39	37.744239811220694	40.0	38.0	41.0	30.0	41.0
40-41	37.69633652322666	40.0	38.0	41.0	30.0	41.0
42-43	37.539912801140716	40.0	37.0	41.0	30.0	41.0
44-45	37.42471769997455	40.0	37.0	41.0	30.0	41.0
46-47	37.321644801644425	40.0	37.0	41.0	30.0	41.0
48-49	37.218351902075966	40.0	36.5	41.0	30.0	41.0
50-51	37.09932868962716	40.0	36.0	41.0	30.0	41.0
52-53	36.97811653215832	40.0	36.0	41.0	30.0	41.0
54-55	36.86872805783027	39.5	35.0	41.0	30.0	41.0
56-57	36.69606671885278	39.0	35.0	41.0	30.0	41.0
58-59	36.517169967482886	39.0	35.0	41.0	29.5	41.0
60-61	36.308230558904846	39.0	35.0	41.0	29.0	41.0
62-63	36.08436172570707	38.0	35.0	40.0	29.0	41.0
64-65	35.793075748924	37.5	35.0	40.0	29.0	41.0
66-67	35.55682418117214	37.0	34.5	40.0	28.5	41.0
68-69	35.30288442412171	36.5	34.0	40.0	28.0	41.0
70-71	35.009615295455234	36.0	34.0	39.0	28.0	41.0
72-73	34.671451855622955	35.5	34.0	39.0	28.0	41.0
74-75	34.33534739406963	35.0	34.0	38.5	27.5	40.0
76-77	34.05570906952488	35.0	34.0	37.0	27.0	39.5
78-79	33.73068408569968	35.0	33.5	37.0	27.0	39.0
80-81	33.483332550641414	35.0	33.0	36.5	27.0	39.0
82-83	33.20632556653684	35.0	33.0	36.0	26.0	38.0
84-85	32.98754846832176	35.0	33.0	36.0	26.0	37.0
86-87	32.779625761591646	35.0	33.0	35.0	26.0	37.0
88-89	32.58685218160167	35.0	33.0	35.0	26.0	36.0
90-91	32.43235298428464	35.0	33.0	35.0	25.5	36.0
92-93	32.276321256247456	35.0	33.0	35.0	25.0	36.0
94-95	32.147701293655146	35.0	33.0	35.0	25.0	36.0
96-97	32.050467925135564	35.0	33.0	35.0	25.0	35.0
98-99	31.937722749384342	35.0	33.0	35.0	25.0	35.0
100	30.810884781866754	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4045449024411809
1101	2	0.37202503040039403
1101	3	0.42354635370709914
1101	4	0.4201443715583224
1101	5	0.26795031930333835
1101	6	0.4200869459087002
1101	7	0.4406278021209715
1101	8	0.5295462589894555
1101	9	0.5243836055786346
1101	10-11	0.5376054057923767
1101	12-13	0.5560354252339295
1101	14-15	0.6110340625226272
1101	16-17	0.6514976636021856
1101	18-19	0.563070083355413
1101	20-21	0.6132741659625509
1101	22-23	0.6376065647414464
1101	24-25	0.6741622056027836
1101	26-27	0.687981189036627
1101	28-29	0.6764997692054422
1101	30-31	0.711403811451369
1101	32-33	0.6368819600256259
1101	34-35	0.6319277642824161
1101	36-37	0.6721831461489032
1101	38-39	0.620738657296755
1101	40-41	0.6358640501065835
1101	42-43	0.6747943316096396
1101	44-45	0.566294577075503
1101	46-47	0.5703929178171308
1101	48-49	0.5918158739608259
1101	50-51	0.6011926648071579
1101	52-53	0.6444907193675675
1101	54-55	0.5987354669912222
1101	56-57	0.5785854176772744
1101	58-59	0.5946743597145812
1101	60-61	0.6403550183258844
1101	62-63	0.5717568323492728
1101	64-65	0.6407127315517798
1101	66-67	0.5888174436909424
1101	68-69	0.5867820820258842
1101	70-71	0.5346282509643032
1101	72-73	0.5694117286384994
1101	74-75	0.5358707057117016
1101	76-77	0.5154748065366732
1101	78-79	0.5641201359107981
1101	80-81	0.5279601218237886
1101	82-83	0.45575564064748164
1101	84-85	0.3707454115874853
1101	86-87	0.4043335818588787
1101	88-89	0.4154514610860929
1101	90-91	0.44932532115892343
1101	92-93	0.42839182873125736
1101	94-95	0.3993014625277169
1101	96-97	0.36284451568587883
1101	98-99	0.4138446138074272
1101	100	0.37432038343952456
1102	1	0.3509034622644158
1102	2	0.35931919369144794
1102	3	0.3102325288565275
1102	4	0.4136042759609566
1102	5	0.39328507113412314
1102	6	0.42627200286246847
1102	7	0.43960095537887156
1102	8	0.5594325793028716
1102	9	0.5111188005034464
1102	10-11	0.5787912348619813
1102	12-13	0.5724672516519362
1102	14-15	0.6497643975193981
1102	16-17	0.6288763526043937
1102	18-19	0.6088185939900512
1102	20-21	0.5618649484012295
1102	22-23	0.6094481060648604
1102	24-25	0.6216468324191595
1102	26-27	0.5285647211470703
1102	28-29	0.5519538080134012
1102	30-31	0.5921560873806158
1102	32-33	0.5812829136171658
1102	34-35	0.6606285942007801
1102	36-37	0.6168667169221038
1102	38-39	0.624017068389044
1102	40-41	0.5767463615283006
1102	42-43	0.6201224715188971
1102	44-45	0.5470461331918628
1102	46-47	0.5165730520793232
1102	48-49	0.5149720939675433
1102	50-51	0.5098067743618486
1102	52-53	0.4664250129830805
1102	54-55	0.4519701248527639
1102	56-57	0.43579025788554304
1102	58-59	0.43502123391936465
1102	60-61	0.45411751963733593
1102	62-63	0.5463691354325491
1102	64-65	0.5515002617412037
1102	66-67	0.48555945664198674
1102	68-69	0.4550158279518186
1102	70-71	0.49158688409451656
1102	72-73	0.479963791979543
1102	74-75	0.5368479941133799
1102	76-77	0.5123740183363807
1102	78-79	0.5373164272276725
1102	80-81	0.4427815818176768
1102	82-83	0.5020704731325196
1102	84-85	0.5101906450642275
1102	86-87	0.4516804137034427
1102	88-89	0.44956947849976103
1102	90-91	0.4180700099496306
1102	92-93	0.40697524629305804
1102	94-95	0.2787946355426669
1102	96-97	0.26729676034727845
1102	98-99	0.33049815295132134
1102	100	0.3353110303255278
1103	1	0.2873864395569612
1103	2	0.3324888131131729
1103	3	0.33332477368746183
1103	4	0.3366167068637509
1103	5	0.3379180629139569
1103	6	0.3343773556610543
1103	7	0.34289060011418115
1103	8	0.3489678419074522
1103	9	0.4297313510329346
1103	10-11	0.425649760038624
1103	12-13	0.46179377242507513
1103	14-15	0.554778857038599
1103	16-17	0.5407576707112298
1103	18-19	0.5593237450574193
1103	20-21	0.51049612317221
1103	22-23	0.49034832764792213
1103	24-25	0.49268318191710136
1103	26-27	0.5144790601471314
1103	28-29	0.4467205964552221
1103	30-31	0.4637068544530507
1103	32-33	0.45398173101452244
1103	34-35	0.4329015042885942
1103	36-37	0.39636308994477787
1103	38-39	0.4113347403172085
1103	40-41	0.43650895649140153
1103	42-43	0.47114311604735093
1103	44-45	0.3700468731241884
1103	46-47	0.46583917347146553
1103	48-49	0.43647934960790735
1103	50-51	0.3840143863636314
1103	52-53	0.30543623452985
1103	54-55	0.2673747831541604
1103	56-57	0.32877144642986167
1103	58-59	0.3299206241445205
1103	60-61	0.2599881617760289
1103	62-63	0.28214070874366115
1103	64-65	0.2626700519165368
1103	66-67	0.2963041625362379
1103	68-69	0.2883696213098048
1103	70-71	0.30437039508631614
1103	72-73	0.2669136296842822
1103	74-75	0.2993451854048317
1103	76-77	0.26359368701091057
1103	78-79	0.23759368369900358
1103	80-81	0.2329170106541696
1103	82-83	0.23929531141735083
1103	84-85	0.2175100007301296
1103	86-87	0.22216217093067314
1103	88-89	0.1672406480906261
1103	90-91	0.25755876153049684
1103	92-93	0.20490673780108892
1103	94-95	0.15887854676427082
1103	96-97	0.22917110750226755
1103	98-99	0.25777786244408674
1103	100	0.24111745641271298
1104	1	0.11729688137090477
1104	2	0.13151814719189758
1104	3	0.16625530457637439
1104	4	0.13265011258010162
1104	5	0.17053357528089208
1104	6	0.14224226233751835
1104	7	0.183734693637696
1104	8	0.31046217083065386
1104	9	0.28429426887387166
1104	10-11	0.31000766865760454
1104	12-13	0.2936037108784504
1104	14-15	0.37729547031628385
1104	16-17	0.3850698486768138
1104	18-19	0.39189140062824634
1104	20-21	0.3808909984248743
1104	22-23	0.3705333094976524
1104	24-25	0.3964224424803575
1104	26-27	0.3986381075162555
1104	28-29	0.36743926242705527
1104	30-31	0.4161559701337936
1104	32-33	0.38761606346146493
1104	34-35	0.3139873090150189
1104	36-37	0.36339440371511245
1104	38-39	0.41248423268204704
1104	40-41	0.3285740395038985
1104	42-43	0.3636476452517101
1104	44-45	0.2919880711535683
1104	46-47	0.25246541988545346
1104	48-49	0.2726263218245748
1104	50-51	0.28336829173838396
1104	52-53	0.28762244820421756
1104	54-55	0.19165111405629887
1104	56-57	0.23592173498310842
1104	58-59	0.24826828787795563
1104	60-61	0.25906512962890815
1104	62-63	0.21904184831154794
1104	64-65	0.30645205092186245
1104	66-67	0.26330267349293024
1104	68-69	0.2503716969008636
1104	70-71	0.21507026402373697
1104	72-73	0.25523554924917846
1104	74-75	0.2207667422616737
1104	76-77	0.16148456831193414
1104	78-79	0.13125709077578307
1104	80-81	0.07296463699425004
1104	82-83	0.08505267169966402
1104	84-85	0.11741969542049446
1104	86-87	0.13763424523564538
1104	88-89	0.1744664667484983
1104	90-91	0.10732066700040122
1104	92-93	0.1286941859763573
1104	94-95	0.11828111553123222
1104	96-97	0.15735422563179924
1104	98-99	0.2029872205943164
1104	100	0.1680825958497607
1105	1	0.09523757324141968
1105	2	0.13769619266676614
1105	3	0.14868415116766087
1105	4	0.20796569406736864
1105	5	0.0800498004289878
1105	6	0.1361903629672483
1105	7	0.1622057440364557
1105	8	0.26636245270254477
1105	9	0.2528961704496382
1105	10-11	0.2831423323270883
1105	12-13	0.25199013484179744
1105	14-15	0.31838143389175855
1105	16-17	0.3326242705367193
1105	18-19	0.2966216633252472
1105	20-21	0.2958427930553853
1105	22-23	0.2598554979790464
1105	24-25	0.3284662774949183
1105	26-27	0.28114953072281423
1105	28-29	0.23578623383941988
1105	30-31	0.33843969897286996
1105	32-33	0.3248036713040605
1105	34-35	0.2991760395017309
1105	36-37	0.3104132127609631
1105	38-39	0.2984119795491509
1105	40-41	0.2579978886855727
1105	42-43	0.30622128525658354
1105	44-45	0.22847119386860015
1105	46-47	0.2710596238243994
1105	48-49	0.3066193887620159
1105	50-51	0.32682376523386125
1105	52-53	0.2510413968454088
1105	54-55	0.23408022876752455
1105	56-57	0.24880723306778663
1105	58-59	0.16014280082944055
1105	60-61	0.19574175277076478
1105	62-63	0.19402262584205943
1105	64-65	0.32076634716325003
1105	66-67	0.2799697437810025
1105	68-69	0.23497421774719385
1105	70-71	0.20350222945529595
1105	72-73	0.26788255032771247
1105	74-75	0.26626435988632124
1105	76-77	0.223899126705426
1105	78-79	0.22366821550431837
1105	80-81	0.14252595386098932
1105	82-83	0.09389522389007254
1105	84-85	0.12673258186997316
1105	86-87	0.1321285321187844
1105	88-89	0.10215867198306228
1105	90-91	0.04772949800614157
1105	92-93	0.153611047174131
1105	94-95	0.19501820836732975
1105	96-97	0.11865303751452672
1105	98-99	0.11082250673450744
1105	100	0.2533205609405442
1106	1	0.1528523143101559
1106	2	0.07213968464218112
1106	3	0.129095775381721
1106	4	0.09916778325082021
1106	5	0.1412671553824012
1106	6	0.1681709690942128
1106	7	0.1585269879250717
1106	8	0.075915606487861
1106	9	0.19330538100487615
1106	10-11	0.22522816485484043
1106	12-13	0.20761400136563424
1106	14-15	0.2547471231095386
1106	16-17	0.2767810181853747
1106	18-19	0.25136035572764825
1106	20-21	0.23637459425449947
1106	22-23	0.26101820139304976
1106	24-25	0.2745546157488832
1106	26-27	0.2518854312435721
1106	28-29	0.2439695740363348
1106	30-31	0.24357359690329616
1106	32-33	0.2438114211395188
1106	34-35	0.23958114434866218
1106	36-37	0.16750901718327071
1106	38-39	0.19114881567374908
1106	40-41	0.11699650228325709
1106	42-43	0.20031439442654175
1106	44-45	0.21657861578653126
1106	46-47	0.17479515483141483
1106	48-49	0.2121250606528875
1106	50-51	0.23888637770019727
1106	52-53	0.19273569409951818
1106	54-55	0.08252847838794963
1106	56-57	0.16397392689847834
1106	58-59	0.11086976002989957
1106	60-61	0.17894303988958882
1106	62-63	0.13992975306137367
1106	64-65	0.26918272570457447
1106	66-67	0.15807184486686054
1106	68-69	0.07741679070749541
1106	70-71	0.14338433341929857
1106	72-73	0.18785198975753303
1106	74-75	0.15535014306261985
1106	76-77	0.1540842734139929
1106	78-79	0.17879657694930984
1106	80-81	0.13353598713381132
1106	82-83	0.19997210763258977
1106	84-85	0.10583477543794118
1106	86-87	0.14559000917014941
1106	88-89	0.14220689124613273
1106	90-91	0.24903600489025024
1106	92-93	0.17182378258362263
1106	94-95	0.20369004490871845
1106	96-97	0.20087096602294707
1106	98-99	0.2124227169135935
1106	100	0.17541146961673704
1107	1	0.02163483513108133
1107	2	0.057032316629459956
1107	3	0.08156884743636539
1107	4	0.14823153823274993
1107	5	0.10952053776489379
1107	6	0.10128479706366988
1107	7	0.12644830180182254
1107	8	0.1476053732052165
1107	9	0.15860850418576433
1107	10-11	0.23793747476268834
1107	12-13	0.23349453776060614
1107	14-15	0.24603403391712675
1107	16-17	0.23570687005025093
1107	18-19	0.22003090444763274
1107	20-21	0.3011883837942335
1107	22-23	0.28943038320495873
1107	24-25	0.29577630217440287
1107	26-27	0.24224139227733588
1107	28-29	0.2409032778196476
1107	30-31	0.2975002315958548
1107	32-33	0.2959794075161497
1107	34-35	0.29935540531229066
1107	36-37	0.23256199736844252
1107	38-39	0.24953123555935264
1107	40-41	0.20445029554775118
1107	42-43	0.23133650442336062
1107	44-45	0.21941633597038646
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2216	62-63	-0.48891846714740694
2216	64-65	-0.38531510393837465
2216	66-67	-0.4640624616444313
2216	68-69	-0.43799011165160096
2216	70-71	-0.48897791385083167
2216	72-73	-0.48668685591937333
2216	74-75	-0.34801201114085245
2216	76-77	-0.4141048101722262
2216	78-79	-0.398442056500663
2216	80-81	-0.41929646757132133
2216	82-83	-0.3808771168152276
2216	84-85	-0.369823049378752
2216	86-87	-0.4136078450250409
2216	88-89	-0.42247959622092424
2216	90-91	-0.4621030013897709
2216	92-93	-0.3921070321731328
2216	94-95	-0.46198167643638754
2216	96-97	-0.38977770076327545
2216	98-99	-0.46232729356206903
2216	100	-0.505847555727378
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1011.0
1	241.0
2	218.0
3	181.0
4	147.0
5	157.0
6	236.0
7	453.0
8	1270.0
9	2190.0
10	2557.0
11	3327.0
12	4504.0
13	5706.0
14	8080.0
15	9785.0
16	10734.0
17	11495.0
18	12822.0
19	14672.0
20	16708.0
21	18537.0
22	20639.0
23	23705.0
24	28283.0
25	34281.0
26	35763.0
27	31753.0
28	33713.0
29	41648.0
30	48514.0
31	54328.0
32	68936.0
33	91831.0
34	133611.0
35	204179.0
36	365041.0
37	751876.0
38	935286.0
39	313953.0
40	792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.408976887096664	20.23608132781375	13.262448253911982	24.0924935311776
2	14.800221375949102	21.397110918150464	40.29308667095889	23.509581034941544
3	17.463607194198946	26.922323894624466	30.8690266133151	24.745042297861485
4	11.968601528609792	18.271916510243123	40.01966895089947	29.739813010247612
5	12.706276258853242	39.05817497938809	35.52699725952923	12.70855150222944
6	29.008328847248976	39.51166451793705	18.649695594974546	12.830311039839422
7	25.57635026120374	33.93828292635608	23.503336563762964	16.982030248677216
8	24.71031304424367	36.37850812153394	21.088354109837784	17.8228247243846
9	25.836418373679088	16.354750054107324	20.833369504664308	36.97546206754929
10-11	23.551240570637795	27.446342728454574	29.213999651228672	19.78841704967896
12-13	24.305809888588037	25.453874394047133	28.457682417462514	21.782633299902308
14-15	22.016735478522932	26.65236393910469	25.97320640997297	25.35769417239941
16-17	21.694489998389397	29.30839531763324	26.832690588624825	22.16442409535254
18-19	21.660771567008798	29.094794100609366	27.951708580999867	21.29272575138197
20-21	22.767787720024277	28.139863635649302	27.367117348026937	21.725231296299484
22-23	22.594966426528647	28.049029646525476	27.154540347812212	22.201463579133662
24-25	22.30923804484572	28.285329607291228	27.373357352035388	22.03207499582766
26-27	22.159345519529793	28.366612635508197	27.115424623540523	22.358617221421483
28-29	22.026885190542796	28.739366390128946	27.102413158852716	22.13133526047554
30-31	22.36126456263484	28.390448734750294	27.000635727147166	22.247650975467696
32-33	22.245038547081446	28.593099636818266	26.66550605786897	22.496355758231314
34-35	22.67789763747231	28.56210680549647	26.952473706696402	21.807521850334822
36-37	22.64501537279189	28.2605639948691	26.924383288258074	22.17003734408094
38-39	22.398408559695028	28.224174048888678	27.174974903588765	22.20244248782753
40-41	22.436189137259294	28.087796705767392	27.294625391660894	22.18138876531242
42-43	22.37676648538811	28.092751906658286	27.01533485136471	22.515146756588894
44-45	22.13499943640585	28.476938071555953	27.19420376720403	22.193858724834172
46-47	22.289570877599182	28.377099980271403	26.880645589102155	22.45268355302726
48-49	22.248902243536964	28.223359083878595	26.980452659495796	22.547286013088648
50-51	22.330315089343774	28.182598771545557	26.914220755347394	22.572865383763276
52-53	22.245674126647767	28.248505022182336	26.922744696187724	22.583076154982173
54-55	22.037268891914643	28.24304265717266	26.981670624499284	22.73801782641341
56-57	22.33301087756771	28.233382525053198	26.900505480101305	22.53310111727779
58-59	22.136001777339164	28.083036710916502	27.171312021232257	22.609649490512073
60-61	22.00097189062368	28.372259858282415	27.045775868728295	22.580992382365604
62-63	22.108789815515813	28.10902845073792	27.05729685577884	22.724884877967426
64-65	21.819680834022254	28.18975096166888	27.035239284656686	22.955328919652178
66-67	22.05195668873611	28.12805277622698	26.922190727240636	22.897799807796275
68-69	22.02622608206168	28.106925404327498	27.081012214784995	22.785836298825828
70-71	22.04141872308814	27.945893093181574	27.015905368157195	22.996782815573095
72-73	22.152424704388967	28.05244415154771	27.054426144885973	22.74070499917735
74-75	22.149627533854467	28.349571570735634	26.98332589503936	22.517475000370542
76-77	22.202324259083657	28.133906738003912	26.981074112743304	22.682694890169135
78-79	22.068195544759764	28.199473405731812	26.90568524778467	22.826645801723753
80-81	22.147179562419716	28.267704275495326	26.992930566243377	22.59218559584158
82-83	22.41248950838296	27.857829643388328	27.313972363516154	22.415708484712557
84-85	22.415943561490675	28.15881520092116	26.834731385746437	22.590509851841727
86-87	22.139505067400915	28.288248932542494	26.93325420751395	22.63899179254264
88-89	22.423019913778173	27.861788777329437	26.76156746655225	22.953623842340136
90-91	22.277477407344477	27.862655816902365	27.00386169170391	22.856005084049244
92-93	22.500090941303114	27.860717743194634	26.970480976950604	22.66871033855164
94-95	22.00224725358128	27.808700218587774	27.20997150664491	22.979081021186037
96-97	22.24237438971541	27.698084737402674	27.05311225494122	23.006428617940703
98-99	22.049686000312008	27.98566198344559	26.87403451795741	23.090617498285003
100	22.392244642779502	27.77538729559503	26.66140927170587	23.170958789919595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3384.0
1	2441.0
2	1559.0
3	1611.5
4	1505.0
5	1278.0
6	1125.5
7	1076.0
8	1020.5
9	963.0
10	903.5
11	864.0
12	874.0
13	936.0
14	972.5
15	1063.0
16	1222.0
17	1496.0
18	1873.0
19	2311.5
20	3132.5
21	4328.0
22	5911.5
23	8216.0
24	11458.0
25	15183.0
26	19735.5
27	25026.0
28	30332.5
29	36417.5
30	43885.0
31	52686.5
32	64093.5
33	71705.0
34	75953.5
35	81685.5
36	88763.5
37	99832.5
38	105775.5
39	104785.5
40	104221.0
41	104657.0
42	104971.0
43	106664.0
44	109333.5
45	116730.5
46	124112.0
47	126605.5
48	127576.5
49	124970.0
50	121448.5
51	120091.5
52	118011.0
53	115686.5
54	112552.0
55	103581.0
56	94025.5
57	83321.5
58	71901.5
59	62501.0
60	51196.5
61	40939.0
62	33390.5
63	25021.5
64	16918.0
65	11725.5
66	8702.5
67	6570.0
68	4827.0
69	3516.5
70	2704.0
71	2020.5
72	1534.0
73	1305.0
74	1058.5
75	824.0
76	610.0
77	446.0
78	327.5
79	227.0
80	164.5
81	123.5
82	91.0
83	56.0
84	41.0
85	30.0
86	24.0
87	18.0
88	13.0
89	10.0
90	9.5
91	10.5
92	9.5
93	6.0
94	3.5
95	3.0
96	2.5
97	6.0
98	7.5
99	5.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09861918189451127
2	0.06700241657376563
3	0.08390257968277347
4	0.08593658161447706
5	0.08557764009711762
6	0.09586729692808876
7	0.09123096899552909
8	0.09248726430628719
9	0.07717242623228363
10-11	0.08571224316612741
12-13	0.07338858440345267
14-15	0.08299026999281818
16-17	0.08378293251032032
18-19	0.08429143299324622
20-21	0.07419620281751145
22-23	0.07937094302611031
24-25	0.08107591523356773
26-27	0.07471965919699398
28-29	0.0840820504414532
30-31	0.08654977337329947
32-33	0.08859873120155973
34-35	0.09135061616798223
36-37	0.08998962958132761
38-39	0.09105149823684935
40-41	0.08677411182164914
42-43	0.09247230840973054
44-45	0.08954095268462829
46-47	0.08477002168305883
48-49	0.09479047237601038
50-51	0.0906626449263766
52-53	0.0966300476524776
54-55	0.08826970147731356
56-57	0.09482038416912367
58-59	0.09987547720526939
60-61	0.09814059320469866
62-63	0.09983060951559944
64-65	0.10158044941272681
66-67	0.10401826055145981
68-69	0.10044380127442185
70-71	0.10294143599938142
72-73	0.10001008027427918
74-75	0.10359949544787378
76-77	0.1000848597570624
78-79	0.10738333727670472
80-81	0.10268718575791846
82-83	0.1074431608629313
84-85	0.10342002468919403
86-87	0.09974087413625958
88-89	0.09839484344616162
90-91	0.09887343213597422
92-93	0.09262186737529698
94-95	0.09230779354760746
96-97	0.09034857109868707
98-99	0.10499039382764166
100	0.24461864408047107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3343163.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.47514281344114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.62009587154203	37.38089522474275
2	15.645234298000519	17.045527454666516
3	6.499954478803387	10.622578455410425
4	3.339400521617125	7.2765728130549086
5	1.8851652503283283	5.13473231192861
6	1.1298072738328733	3.6927847556226223
7	0.732117243460786	2.7917533975596416
8	0.49926073326626236	2.1757839796658387
9	0.3523415276043906	1.7274469531819672
>10	1.2672123040765064	10.684390734846584
>50	0.024662057456769082	0.910314373191572
>100	0.00465541821340194	0.3828511478792462
>500	0.0	0.0
>1k	9.302179765433665E-5	0.17436839824933548
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3441	0.10292648010282479	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.973537933986468E-5
2	0.0	0.0	0.0	0.0	8.973537933986468E-5
3	0.0	0.0	0.0	0.0	8.973537933986468E-5
4	0.0	0.0	0.0	0.0	8.973537933986468E-5
5	0.0	0.0	0.0	2.9911793113288225E-5	8.973537933986468E-5
6	0.0	0.0	0.0	2.9911793113288225E-5	1.196471724531529E-4
7	0.0	0.0	0.0	2.9911793113288225E-5	1.196471724531529E-4
8	0.0	0.0	0.0	2.9911793113288225E-5	1.196471724531529E-4
9	0.0	0.0	0.0	2.9911793113288225E-5	1.196471724531529E-4
10-11	0.0	0.0	0.0	2.9911793113288225E-5	1.196471724531529E-4
12-13	0.0	0.0	0.0	7.477948278322057E-5	4.636327932559675E-4
14-15	0.0	0.0	0.0	8.973537933986468E-5	5.533681725958322E-4
16-17	0.0	0.0	0.0	8.973537933986468E-5	7.477948278322056E-4
18-19	0.0	0.0	0.0	1.3460306900979702E-4	7.777066209454939E-4
20-21	0.0	0.0	0.0	2.6920613801959404E-4	9.422214830685791E-4
22-23	0.0	0.0	0.0	6.131917588224087E-4	9.571773796252232E-4
24-25	0.0	0.0	0.0	0.0012413394142014613	9.571773796252232E-4
26-27	0.0	0.0	0.0	0.0024976347249595666	0.0010768245520783762
28-29	0.0	0.0	0.0	0.006849800622943003	0.0011067363451916644
30-31	0.0	0.0	0.0	0.01887434145448487	0.0011216922417483085
32-33	0.0	0.0	0.0	0.04480786608370576	0.0011366481383049524
34-35	0.0	0.0	0.0	0.0840820504414532	0.001196471724531529
36-37	0.0	0.0	0.0	0.1434120920816604	0.001196471724531529
38-39	0.0	0.0	0.0	0.23283339759383553	0.0012862071038713936
40-41	0.0	0.0	0.0	0.33139275590212025	0.0012862071038713936
42-43	0.0	0.0	0.0	0.43527641338457024	0.0013011630004280377
44-45	0.0	0.0	0.0	0.5642710211856257	0.0013759424832112583
46-47	0.0	0.0	0.0	0.7221604211341176	0.0014806337591077672
48-49	0.0	0.0	0.0	0.893644731052599	0.00160028093156092
50-51	0.0	0.0	0.0	1.0857083546330226	0.001734884000570717
52-53	0.0	0.0	0.0	1.2705034124869172	0.0018246193799105817
54-55	0.0	0.0	0.0	1.4728566928983122	0.00185453117302387
56-57	0.0	0.0	0.0	1.7126296264944305	0.0019143547592504464
58-59	0.0	0.0	0.0	1.9848718115150232	0.001974178345477023
60-61	0.0	0.0	0.0	2.2816715786816255	0.0020190460351469553
62-63	0.0	0.0	0.0	2.583646086056827	0.002123737311043464
64-65	0.0	0.0	0.0	2.896059809228566	0.002138693207600108
66-67	0.0	0.0	0.0	3.252144750345706	0.0021686050007133965
68-69	0.0	0.0	0.0	3.6609342709284594	0.0021835608972700406
70-71	0.0	0.0	0.0	4.104182177177721	0.002243384483496617
72-73	0.0	0.0	0.0	4.556792474671441	0.0023480757593931255
74-75	0.0	0.0	0.0	5.003599884301185	0.00243781113873299
76-77	0.0	0.0	0.0	5.486765078460129	0.0024826788284029225
78-79	0.0	0.0	0.0	6.026328958534179	0.0024826788284029225
80-81	0.0	0.0	0.0	6.6198537133846	0.0024826788284029225
82-83	0.0	0.0	0.0	7.239715802071272	0.002527546518072855
84-85	0.0	0.0	0.0	7.840673637510346	0.0025873701042994314
86-87	0.0	0.0	0.0	8.466622776095573	0.0027369290698658726
88	0.0	0.0	0.0	8.949758058461404	0.0028715321388756696
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3835	0.0	38.9948	1
GTATCAA	6235	0.0	33.337326	1
GTACTAG	1125	0.0	30.93315	1
CAACGCA	6560	0.0	29.597023	5
ATCAACG	6625	0.0	29.237429	3
AACGCAG	7075	0.0	27.632849	6
TCAACGC	7105	0.0	27.460306	4
TATCAAC	7525	0.0	27.240044	2
GTACATG	10455	0.0	25.998528	1
TACATGG	10555	0.0	24.587177	2
ACGCAGA	7925	0.0	24.19467	7
CGCAGAG	7945	0.0	23.95631	8
ACATGGG	10250	0.0	23.85104	3
CATGGGG	7050	0.0	22.539742	4
TGATCGC	705	0.0	21.330456	9
GCAGAGT	8715	0.0	20.814173	9
CGTTAAC	275	1.4424586E-9	20.506323	9
GTGATCG	715	0.0	20.37579	8
TATTCGC	215	1.1969369E-6	19.671766	9
CTAGGCA	1900	0.0	19.547663	4
>>END_MODULE