FastQCFastQC Report
Mon 16 Jan 2017
ERR1146513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3343163
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175770.5257595875522671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159110.47592654022552894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93710.28030341326462394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84000.061.2539371
GTATCAA154300.041.8200151
ATCAACG195700.032.539983
CAACGCA197250.032.287835
TATCAAC201650.031.8135492
TCAACGC202950.031.3543824
AACGCAG207100.031.0453516
ACGCAGA227600.027.9591547
CGCAGAG229450.027.5698628
GTGGTAT27350.027.514311
GTACTAG11650.027.4523181
GCAGAGT247800.025.338619
TGGTATC29850.024.5796812
GTACATG103750.024.0261251
TACATGG105050.023.1915472
GAGTACT187800.023.12352412-13
ACATGGG100100.022.8814953
CAGAGTA242850.022.62319810-11
AGAGTAC235300.022.13071610-11
CATGGGG67750.021.8670794
Produced by FastQC (version 0.11.5)