##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146513_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3343163 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38129489947095 33.0 31.0 34.0 30.0 34.0 2 32.45918401226623 34.0 31.0 34.0 30.0 34.0 3 32.52971183277633 34.0 31.0 34.0 30.0 34.0 4 36.05149165625487 37.0 35.0 37.0 35.0 37.0 5 36.09577277566185 37.0 35.0 37.0 35.0 37.0 6 36.15757203582356 37.0 36.0 37.0 35.0 37.0 7 36.12787500938482 37.0 36.0 37.0 35.0 37.0 8 36.129029903716926 37.0 36.0 37.0 35.0 37.0 9 37.91589910512889 39.0 38.0 39.0 35.0 39.0 10-11 37.860149654683305 39.0 38.0 39.0 35.0 39.0 12-13 37.830251471435886 39.0 38.0 39.0 35.0 39.0 14-15 39.17011629406045 40.0 39.0 41.0 36.0 41.0 16-17 39.11977758787113 40.0 39.0 41.0 36.0 41.0 18-19 39.24358145265427 40.5 39.0 41.0 36.0 41.0 20-21 39.30426664808147 41.0 39.0 41.0 36.0 41.0 22-23 39.26772744852704 41.0 39.0 41.0 36.0 41.0 24-25 39.248149880816456 41.0 39.0 41.0 36.0 41.0 26-27 39.16097001552123 41.0 39.0 41.0 35.5 41.0 28-29 39.08835210846734 40.5 39.0 41.0 35.0 41.0 30-31 38.908089734182866 40.0 38.5 41.0 35.0 41.0 32-33 38.751579417455865 40.0 38.0 41.0 35.0 41.0 34-35 38.65635357893109 40.0 38.0 41.0 35.0 41.0 36-37 38.5608802203183 40.0 38.0 41.0 35.0 41.0 38-39 38.45956568674636 40.0 38.0 41.0 34.5 41.0 40-41 38.340264145062626 40.0 38.0 41.0 34.0 41.0 42-43 38.21462519177199 40.0 38.0 41.0 34.0 41.0 44-45 38.04463348032986 40.0 38.0 41.0 33.0 41.0 46-47 37.762993309030996 40.0 37.5 41.0 33.0 41.0 48-49 37.434244456522165 40.0 37.0 41.0 32.0 41.0 50-51 37.30828365233762 40.0 37.0 41.0 32.0 41.0 52-53 37.20789354273184 40.0 36.5 41.0 31.0 41.0 54-55 37.04778872582641 40.0 36.0 41.0 31.0 41.0 56-57 36.89848311314764 39.5 36.0 41.0 31.0 41.0 58-59 36.723098305407184 39.0 35.0 41.0 31.0 41.0 60-61 36.52421793373521 39.0 35.0 41.0 31.0 41.0 62-63 36.29749760929994 39.0 35.0 41.0 31.0 41.0 64-65 36.036818127025214 38.0 35.0 40.5 30.0 41.0 66-67 35.77858827104751 37.0 35.0 40.0 30.0 41.0 68-69 35.50015195190902 37.0 35.0 40.0 30.0 41.0 70-71 35.19946275428389 36.0 35.0 39.0 29.0 41.0 72-73 34.88054052404863 36.0 35.0 39.0 29.0 41.0 74-75 34.56960997713841 35.5 34.5 39.0 29.0 40.5 76-77 34.27226431974749 35.0 34.0 37.5 29.0 39.5 78-79 33.9842399248855 35.0 34.0 37.0 29.0 39.0 80-81 33.7279902296119 35.0 34.0 36.5 29.0 39.0 82-83 33.473078638403216 35.0 34.0 36.0 29.0 38.0 84-85 33.25572818316068 35.0 34.0 36.0 29.0 37.0 86-87 33.06156161096543 35.0 34.0 35.5 28.5 37.0 88-89 32.86009775772225 35.0 34.0 35.0 27.0 36.5 90-91 32.76842424374762 35.0 34.0 35.0 27.0 36.0 92-93 32.66287405071186 35.0 34.0 35.0 27.0 36.0 94-95 32.584032396864885 35.0 34.0 35.0 27.0 36.0 96-97 32.5276314675653 35.0 34.0 35.0 27.0 35.5 98-99 32.450231562146385 35.0 34.0 35.0 27.0 35.0 100 31.74186571220129 35.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28760816267764966 1101 2 0.3246703136260507 1101 3 0.2919247423327107 1101 4 0.2332404401264725 1101 5 0.2164406548822697 1101 6 0.24284129046038316 1101 7 0.27150040663142505 1101 8 0.2609233599091709 1101 9 0.25486627363447667 1101 10-11 0.33198939009914596 1101 12-13 0.30864432716555257 1101 14-15 0.4599644976016606 1101 16-17 0.49046703366735755 1101 18-19 0.46317602997146423 1101 20-21 0.3973135142287205 1101 22-23 0.3835761340504362 1101 24-25 0.40624897825397 1101 26-27 0.4339183795280306 1101 28-29 0.42512840520391393 1101 30-31 0.467026981717396 1101 32-33 0.4587041098355371 1101 34-35 0.4468410911092633 1101 36-37 0.4164105370837561 1101 38-39 0.46930042089611845 1101 40-41 0.4060234391309976 1101 42-43 0.39095145584969515 1101 44-45 0.476102602379747 1101 46-47 0.4938368814997105 1101 48-49 0.5184243841305332 1101 50-51 0.5081940457227603 1101 52-53 0.4651800388005185 1101 54-55 0.5099559037032577 1101 56-57 0.5024226352636632 1101 58-59 0.48597766526432196 1101 60-61 0.4433346097151727 1101 62-63 0.5126549448686646 1101 64-65 0.43659139532059044 1101 66-67 0.45335824722659623 1101 68-69 0.3915651002141658 1101 70-71 0.34936571263514793 1101 72-73 0.38605845146733486 1101 74-75 0.41521581463353385 1101 76-77 0.3847431579338547 1101 78-79 0.39864309952584875 1101 80-81 0.27349293202291847 1101 82-83 0.33171304077262675 1101 84-85 0.35625944158130807 1101 86-87 0.36401746285819314 1101 88-89 0.37864379784347335 1101 90-91 0.35170376389284996 1101 92-93 0.34972378249454295 1101 94-95 0.3207416766104316 1101 96-97 0.30927649342319796 1101 98-99 0.30188534499178843 1101 100 0.3238024309474703 1102 1 0.33168919310170253 1102 2 0.30942011961317206 1102 3 0.3321794235244724 1102 4 0.21180192669854847 1102 5 0.1668343614598129 1102 6 0.21748445746308676 1102 7 0.22747240414947356 1102 8 0.22600824034824996 1102 9 0.2591998579184036 1102 10-11 0.277962125037007 1102 12-13 0.3010371736961659 1102 14-15 0.46538285026171167 1102 16-17 0.5074217083445589 1102 18-19 0.40488084413183145 1102 20-21 0.38498102219612207 1102 22-23 0.3667788601837927 1102 24-25 0.39794601429235854 1102 26-27 0.4073457618523477 1102 28-29 0.41190634872853593 1102 30-31 0.4409347226265936 1102 32-33 0.4219997891871756 1102 34-35 0.45421241523458633 1102 36-37 0.4126920044290543 1102 38-39 0.43796336690694204 1102 40-41 0.39903571313175945 1102 42-43 0.42705077117818036 1102 44-45 0.43033959191718907 1102 46-47 0.415174460589796 1102 48-49 0.47779454578393654 1102 50-51 0.4933086518447709 1102 52-53 0.513190522590385 1102 54-55 0.5009695576733577 1102 56-57 0.5182295190983908 1102 58-59 0.4914230756389699 1102 60-61 0.5051736829607449 1102 62-63 0.5117492752598807 1102 64-65 0.44822792150568347 1102 66-67 0.42493130870058593 1102 68-69 0.4111597015968087 1102 70-71 0.42979325456113315 1102 72-73 0.48200301013244484 1102 74-75 0.4714062209881078 1102 76-77 0.47980519492975304 1102 78-79 0.45105671498855315 1102 80-81 0.45393311469175046 1102 82-83 0.40053864896014346 1102 84-85 0.3730641466097566 1102 86-87 0.37361637483253674 1102 88-89 0.3518872653861749 1102 90-91 0.3226805976213569 1102 92-93 0.3482951351638093 1102 94-95 0.3914171262371511 1102 96-97 0.3879322599477959 1102 98-99 0.3595383955950453 1102 100 0.4994871620176049 1103 1 0.23605641015294765 1103 2 0.2147647077449406 1103 3 0.23473979563646452 1103 4 0.19978595915323183 1103 5 0.16124118898656548 1103 6 0.1775768488525813 1103 7 0.2007537273729767 1103 8 0.17130550533545374 1103 9 0.20959662704583337 1103 10-11 0.22436911498792966 1103 12-13 0.20715917665787487 1103 14-15 0.3583672367177968 1103 16-17 0.41546811049053645 1103 18-19 0.2955977871939268 1103 20-21 0.3403417684588206 1103 22-23 0.33059059649023226 1103 24-25 0.33138269554018507 1103 26-27 0.3429847879181551 1103 28-29 0.31295306739341555 1103 30-31 0.3606549157586585 1103 32-33 0.3324460381903691 1103 34-35 0.28387517387128014 1103 36-37 0.30427736646142733 1103 38-39 0.27971795053090176 1103 40-41 0.36178819694239195 1103 42-43 0.3775759029965968 1103 44-45 0.3802424943914886 1103 46-47 0.34942789841932154 1103 48-49 0.46077952323394555 1103 50-51 0.3948586573679904 1103 52-53 0.41224300860236696 1103 54-55 0.36624071679291603 1103 56-57 0.28355842407588483 1103 58-59 0.2652922642895845 1103 60-61 0.2921687624405038 1103 62-63 0.3205588974739584 1103 64-65 0.22545102548114215 1103 66-67 0.23172106538758896 1103 68-69 0.21589679556861086 1103 70-71 0.2128938724671059 1103 72-73 0.27972135710260204 1103 74-75 0.27589980265523906 1103 76-77 0.2186321622206222 1103 78-79 0.25334291946492016 1103 80-81 0.19347802556967508 1103 82-83 0.21612935631442554 1103 84-85 0.18084585090534944 1103 86-87 0.23428291285556213 1103 88-89 0.2593285185074947 1103 90-91 0.2492414215065537 1103 92-93 0.24130892257615244 1103 94-95 0.24975080002000283 1103 96-97 0.18141994317821997 1103 98-99 0.22955513182910892 1103 100 0.22269728832173286 1104 1 0.19323935283853189 1104 2 0.15713118635453327 1104 3 0.1386325696505395 1104 4 0.08995637766881259 1104 5 0.07432915851284605 1104 6 0.10994804065964559 1104 7 0.14980714782364402 1104 8 0.13730562130687218 1104 9 0.16566836280346564 1104 10-11 0.19905872417167103 1104 12-13 0.1936015786341514 1104 14-15 0.3157966186719037 1104 16-17 0.40737780295068404 1104 18-19 0.3088695454024375 1104 20-21 0.28865810132356273 1104 22-23 0.26957113708060376 1104 24-25 0.2577564550734337 1104 26-27 0.2513332619647244 1104 28-29 0.24237479374695425 1104 30-31 0.3456556665992565 1104 32-33 0.3017888816757832 1104 34-35 0.35420803043321314 1104 36-37 0.3220894801223366 1104 38-39 0.28041731023484573 1104 40-41 0.28777668603550666 1104 42-43 0.25407535399086356 1104 44-45 0.25194084008890627 1104 46-47 0.34939165735042366 1104 48-49 0.44107200166958194 1104 50-51 0.3644698706148972 1104 52-53 0.2808279677038641 1104 54-55 0.3048501116674345 1104 56-57 0.300944751640138 1104 58-59 0.24037476383053047 1104 60-61 0.20967080123293869 1104 62-63 0.29513262816304575 1104 64-65 0.27212970807554626 1104 66-67 0.23260897157562965 1104 68-69 0.18701647361410778 1104 70-71 0.2644689093214794 1104 72-73 0.23979633708637493 1104 74-75 0.19259738264391757 1104 76-77 0.1948745618179899 1104 78-79 0.21799338086018594 1104 80-81 0.21328562823968866 1104 82-83 0.2718749967926186 1104 84-85 0.23360355464149052 1104 86-87 0.235198882640546 1104 88-89 0.21276311248485769 1104 90-91 0.2315634927263872 1104 92-93 0.23119153124917347 1104 94-95 0.26404799462674333 1104 96-97 0.2635075439748533 1104 98-99 0.27352697827996764 1104 100 0.307339141069253 1105 1 0.176311557536863 1105 2 0.11800976348528991 1105 3 0.1392590189036511 1105 4 0.11613154301664963 1105 5 0.07646559869312597 1105 6 0.11526446975187099 1105 7 0.09179088072774988 1105 8 0.10303414208919293 1105 9 0.15168244861394697 1105 10-11 0.14889125732556607 1105 12-13 0.09607020643957043 1105 14-15 0.23040245209676868 1105 16-17 0.2753043017627377 1105 18-19 0.2637691128644448 1105 20-21 0.23925698179309762 1105 22-23 0.27197969737169814 1105 24-25 0.21617559399329878 1105 26-27 0.2196950448411954 1105 28-29 0.21947382687186945 1105 30-31 0.23847018209356463 1105 32-33 0.2444000800940529 1105 34-35 0.2839992904152737 1105 36-37 0.22840482895069414 1105 38-39 0.20419747264633514 1105 40-41 0.22441685351532925 1105 42-43 0.23464005349328687 1105 44-45 0.2891237774552309 1105 46-47 0.3408938771240031 1105 48-49 0.3375009901049637 1105 50-51 0.38530116782238366 1105 52-53 0.2820028735807867 1105 54-55 0.29089217709042714 1105 56-57 0.2753157941682858 1105 58-59 0.22852639257264684 1105 60-61 0.1896154950097042 1105 62-63 0.27537668649556224 1105 64-65 0.2530617102654773 1105 66-67 0.24771214585618395 1105 68-69 0.2045398627538617 1105 70-71 0.194500006909351 1105 72-73 0.2205266438934217 1105 74-75 0.20988188557118548 1105 76-77 0.20208432847580582 1105 78-79 0.2368428176785855 1105 80-81 0.2155013584037846 1105 82-83 0.22339147449238794 1105 84-85 0.15216022178962163 1105 86-87 0.14024911620760605 1105 88-89 0.2024641068638502 1105 90-91 0.1718913904325774 1105 92-93 0.21344964598548444 1105 94-95 0.27988728786259287 1105 96-97 0.17730804458071958 1105 98-99 0.15822578445433777 1105 100 0.20098357425073843 1106 1 0.023258092505678007 1106 2 0.009881871086363958 1106 3 0.07198350472589254 1106 4 0.056069758327460306 1106 5 0.06997642304055773 1106 6 0.07912084129276309 1106 7 0.04159519603453532 1106 8 0.036087955483509404 1106 9 0.12510648907051802 1106 10-11 0.09599111354282996 1106 12-13 0.056585381593642126 1106 14-15 0.15206354702696245 1106 16-17 0.20265794051930186 1106 18-19 0.1507957174395287 1106 20-21 0.09531435305322589 1106 22-23 0.0940061796049676 1106 24-25 0.1059005198313514 1106 26-27 0.1519927858307213 1106 28-29 0.0951143484710073 1106 30-31 0.11842963926086725 1106 32-33 0.1426452837765808 1106 34-35 0.13074261355899353 1106 36-37 0.06825620231012408 1106 38-39 0.09270298238246255 1106 40-41 0.05984413955504664 1106 42-43 0.15718176064680023 1106 44-45 0.16550618810319406 1106 46-47 0.15104499207264155 1106 48-49 0.26409190023266405 1106 50-51 0.22581399567252447 1106 52-53 0.12147448965807683 1106 54-55 0.19502770361847865 1106 56-57 0.1804952488721696 1106 58-59 0.10857909908458652 1106 60-61 0.10887079002558409 1106 62-63 0.13811701039862356 1106 64-65 0.21128268612184087 1106 66-67 0.18556045196120863 1106 68-69 0.14978943383176357 1106 70-71 0.17581169362141225 1106 72-73 0.16778355382123777 1106 74-75 0.16207636592329777 1106 76-77 0.17875938488781884 1106 78-79 0.20068651958425932 1106 80-81 0.20748087796955872 1106 82-83 0.09797434074955902 1106 84-85 0.11231508993625283 1106 86-87 0.10890591092675095 1106 88-89 0.15295449017526863 1106 90-91 0.17337104208603193 1106 92-93 0.15104928717146038 1106 94-95 0.2323018175910292 1106 96-97 0.17588357115855047 1106 98-99 0.16136799913975608 1106 100 0.26489539358962233 1107 1 0.10023152388907874 1107 2 0.05392008948153659 1107 3 0.09562550375658674 1107 4 0.09273431895848461 1107 5 0.05277061848789799 1107 6 0.06200128046753406 1107 7 0.06048064241156936 1107 8 0.05042298980851001 1107 9 0.11989953910467221 1107 10-11 0.11959921818554875 1107 12-13 0.1649829514263388 1107 14-15 0.23415466913385075 1107 16-17 0.3012767381780037 1107 18-19 0.22217627325441214 1107 20-21 0.2153432465112246 1107 22-23 0.14484227334067867 1107 24-25 0.1866481094096315 1107 26-27 0.19699681335247732 1107 28-29 0.2060754678687502 1107 30-31 0.2629449362444589 1107 32-33 0.24307375226408823 1107 34-35 0.23146491382273382 1107 36-37 0.24392442232885259 1107 38-39 0.16846272931079653 1107 40-41 0.19058058379009424 1107 42-43 0.17535542487620148 1107 44-45 0.21904092647350382 1107 46-47 0.2254566228896664 1107 48-49 0.31744317467371985 1107 50-51 0.31490008717675266 1107 52-53 0.3051152487090292 1107 54-55 0.28509305344596214 1107 56-57 0.17623999542296076 1107 58-59 0.21063721215868014 1107 60-61 0.1610965536685569 1107 62-63 0.22306840874114897 1107 64-65 0.23163150159138723 1107 66-67 0.22538243767056088 1107 68-69 0.22260863151662136 1107 70-71 0.11629206273974546 1107 72-73 0.10843725473860388 1107 74-75 0.17103383244685944 1107 76-77 0.08523017335331673 1107 78-79 0.10121511522999782 1107 80-81 0.16181223095392028 1107 82-83 0.10202001591008525 1107 84-85 0.09760341779460902 1107 86-87 0.11841099496432861 1107 88-89 0.21883468642015913 1107 90-91 0.14909524212420422 1107 92-93 0.21067259145694095 1107 94-95 0.23083875005000465 1107 96-97 0.19506236682942557 1107 98-99 0.19546533127749655 1107 100 0.16912010590346327 1108 1 0.052663011165975604 1108 2 0.018969163974624337 1108 3 0.008160255560774488 1108 4 -0.004308463914767913 1108 5 0.02208551604370257 1108 6 0.01483738414847835 1108 7 0.04658416991592418 1108 8 0.006516326361776237 1108 9 0.04876979297667816 1108 10-11 0.06310063965443646 1108 12-13 0.06800420134164398 1108 14-15 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76-77 -0.19132815080046583 2214 78-79 -0.19471705437775455 2214 80-81 -0.16221703540455223 2214 82-83 -0.20534104449711776 2214 84-85 -0.1687290193183415 2214 86-87 -0.14301332092276908 2214 88-89 -0.08859387458922896 2214 90-91 -0.09424071261739897 2214 92-93 -0.08138652671664914 2214 94-95 -0.07290723773534324 2214 96-97 -0.08039961343575897 2214 98-99 -0.03529095227379031 2214 100 -0.25274141503391334 2215 1 -0.44112606102435237 2215 2 -0.4236654152685446 2215 3 -0.447024360638153 2215 4 -0.2773749504898433 2215 5 -0.2644507882906808 2215 6 -0.2459641892295963 2215 7 -0.27383001523022443 2215 8 -0.3154103347822499 2215 9 -0.40826210121824147 2215 10-11 -0.3774767566828885 2215 12-13 -0.37142578071934196 2215 14-15 -0.48820983449304833 2215 16-17 -0.46259880636508655 2215 18-19 -0.4903823536465737 2215 20-21 -0.5657627176086208 2215 22-23 -0.5153616130033214 2215 24-25 -0.4787244497831509 2215 26-27 -0.5074901295349932 2215 28-29 -0.5076092024583474 2215 30-31 -0.4462153570863592 2215 32-33 -0.5227821758522495 2215 34-35 -0.5033930924827246 2215 36-37 -0.5419001469722602 2215 38-39 -0.5505230876922056 2215 40-41 -0.5406130603909887 2215 42-43 -0.576916928850217 2215 44-45 -0.523337918152535 2215 46-47 -0.49926371808976455 2215 48-49 -0.3512557587641467 2215 50-51 -0.37684679657277087 2215 52-53 -0.3760738811251727 2215 54-55 -0.3262900788558767 2215 56-57 -0.3993376422829016 2215 58-59 -0.3938579646431748 2215 60-61 -0.3494185046984555 2215 62-63 -0.2979415682833704 2215 64-65 -0.29865286729179985 2215 66-67 -0.3648006222745579 2215 68-69 -0.2976416635849688 2215 70-71 -0.19135290422445905 2215 72-73 -0.21200655324651052 2215 74-75 -0.27249079792509434 2215 76-77 -0.27313820180290094 2215 78-79 -0.2920569342548731 2215 80-81 -0.3261227478270641 2215 82-83 -0.32597376700768166 2215 84-85 -0.27220137167309844 2215 86-87 -0.30750369278116096 2215 88-89 -0.22541419460996082 2215 90-91 -0.2986543559558683 2215 92-93 -0.2950283928296358 2215 94-95 -0.3630865921899371 2215 96-97 -0.2866858267165213 2215 98-99 -0.25311961172251785 2215 100 -0.4369414221012242 2216 1 -0.5156979755662654 2216 2 -0.49677475837112794 2216 3 -0.4731938455324851 2216 4 -0.32826520079024846 2216 5 -0.2774049229700495 2216 6 -0.3257678909522852 2216 7 -0.35133508681101233 2216 8 -0.36062719975030433 2216 9 -0.4267382739420853 2216 10-11 -0.43256338002530015 2216 12-13 -0.4308265442921382 2216 14-15 -0.4824400103923381 2216 16-17 -0.4995098648052121 2216 18-19 -0.49442684170944773 2216 20-21 -0.4871533049902368 2216 22-23 -0.487854569887908 2216 24-25 -0.49707512280618005 2216 26-27 -0.4900818116560117 2216 28-29 -0.5546412107070466 2216 30-31 -0.551413922628484 2216 32-33 -0.5993519718905915 2216 34-35 -0.5302651862294567 2216 36-37 -0.5026439737004793 2216 38-39 -0.5182634555293575 2216 40-41 -0.5335213351266432 2216 42-43 -0.5502246544238645 2216 44-45 -0.6099669703283013 2216 46-47 -0.5735199310482813 2216 48-49 -0.46755874983821144 2216 50-51 -0.5193132171133925 2216 52-53 -0.45194972257998955 2216 54-55 -0.49243395172312887 2216 56-57 -0.5054702159523643 2216 58-59 -0.6331979409386719 2216 60-61 -0.589923478726682 2216 62-63 -0.5802979848732406 2216 64-65 -0.518674646348309 2216 66-67 -0.5147766053982252 2216 68-69 -0.5196027029882586 2216 70-71 -0.48189052379355246 2216 72-73 -0.45980166061450234 2216 74-75 -0.4510083421508426 2216 76-77 -0.43063363078707795 2216 78-79 -0.5081905525816452 2216 80-81 -0.5096655417783893 2216 82-83 -0.44969934270219625 2216 84-85 -0.37981875415619015 2216 86-87 -0.37445440979245603 2216 88-89 -0.41600478957045794 2216 90-91 -0.3802506819370848 2216 92-93 -0.33391010254445774 2216 94-95 -0.36099735438778424 2216 96-97 -0.37445612859431776 2216 98-99 -0.3622305981120135 2216 100 -0.5160732145821001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 11.0 8 130.0 9 404.0 10 514.0 11 360.0 12 300.0 13 374.0 14 566.0 15 1096.0 16 2048.0 17 3659.0 18 6305.0 19 9242.0 20 13140.0 21 19447.0 22 27425.0 23 31393.0 24 29728.0 25 25233.0 26 22107.0 27 23458.0 28 27396.0 29 33815.0 30 41241.0 31 51770.0 32 65316.0 33 84817.0 34 121404.0 35 190927.0 36 329587.0 37 742699.0 38 1041322.0 39 394516.0 40 1413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8535447956519 19.897968035368688 13.759119611614473 23.489367557364936 2 15.26145286489882 21.215776012922333 40.138915062592545 23.383856059586307 3 17.31923886269108 27.054294521193306 31.68228813800138 23.944178478114235 4 11.856224059001454 18.388395311414683 39.712229053200126 30.043151576383735 5 12.18062232730214 38.50321142520753 35.70027376083736 13.615892486652964 6 28.49845722174278 39.65165966446256 18.202868984249154 13.647014129545513 7 24.695056747158304 34.51590604466489 23.508964414837084 17.28007279333972 8 25.268474343059687 34.97324379701779 20.995049578989875 18.763232280932655 9 26.275924924988704 14.728596840776234 21.19343268635122 37.80204554788384 10-11 23.836766363789458 26.574252077152178 29.127510836307813 20.461470722750548 12-13 25.308158770601374 24.845662625483712 28.94218738362443 20.903991220290486 14-15 22.47608028684213 25.476173312518714 27.666763481170374 24.380982919468778 16-17 20.895944502697827 28.337750569933444 28.366391193225198 22.399913734143524 18-19 20.73287625033408 26.801542195227775 31.540072981925917 20.925508572512236 20-21 21.74849868814216 25.852969324377135 31.541809476933423 20.856722510547282 22-23 21.55677123729833 25.840887805949038 31.332378947721068 21.269962009031566 24-25 21.268496331169015 26.08208154971804 31.54132777851394 21.108094340599006 26-27 21.236749063760037 26.16965685160148 31.27007083118965 21.323523253448833 28-29 21.0547317016849 26.44959279580445 31.322792218028255 21.172883284482392 30-31 21.441861905058886 26.174873955197732 31.10123621194521 21.282027927798175 32-33 21.409294262579124 26.295347035885484 30.75512499669099 21.5402337048444 34-35 21.75264798243936 26.348720827923657 30.943627111906302 20.955004077730685 36-37 21.77005534582391 26.09999937185197 30.80565070004107 21.324294582283056 38-39 21.671236720198163 26.093634698626232 30.897739580831317 21.337389000344288 40-41 21.676905256341133 26.078798379673078 30.792582749366055 21.451713614619734 42-43 21.801584308034037 26.07310801178405 30.3862539756512 21.739053704530708 44-45 21.64705365052164 26.49639389012533 30.312754859158836 21.54379760019419 46-47 22.004999457100954 26.49830415088944 29.595490857011757 21.901205534997846 48-49 22.184049766074683 26.631188267630545 29.21930771506368 21.965454251231094 50-51 22.18448517167724 26.798349347608834 28.876336571085524 22.140828909628397 52-53 22.057404253085302 26.962285114680036 28.76586883117252 22.214441801062144 54-55 21.931272663859534 26.953298278601068 28.690759890438756 22.42466916710064 56-57 22.192087725385566 26.905248327929264 28.581536630014714 22.321127316670456 58-59 21.920055516794505 26.795812011638787 28.922739376916713 22.361393094650005 60-61 21.82877717144612 26.994988104768964 28.860760364524857 22.315474359260058 62-63 21.863256795896813 26.72270551457799 28.868895478136388 22.545142211388818 64-65 21.520582515966215 26.73585384814874 28.950948401105187 22.792615234779863 66-67 21.693428569633845 26.704611227286584 28.812385903839015 22.789574299240563 68-69 21.658809182024928 26.638622809927444 28.950162080250735 22.75240592779689 70-71 21.758165924783484 26.456109640572777 28.794395209910416 22.99132922473332 72-73 21.780931959209415 26.527010831792463 28.81794024693729 22.874116962060835 74-75 21.819357108201352 26.82499262656435 28.573274601101616 22.78237566413268 76-77 21.827471372707148 26.65352697281615 28.49765079862676 23.02135085584994 78-79 21.638753179283945 26.74596268702924 28.423583668822634 23.19170046486418 80-81 21.67715259694469 26.91176727920134 28.366960248784785 23.044119875069192 82-83 21.90792744319273 26.46417476233464 28.72198821110415 22.90590958336848 84-85 21.893529974372953 26.730229521730642 28.190694547578293 23.18554595631811 86-87 21.596826358276033 26.87829263459374 28.26506339431601 23.259817612814217 88-89 21.791593769134202 26.56529161156665 28.04514766405347 23.597966955245674 90-91 21.588337865221288 26.59425815952953 28.24313153881438 23.574272436434793 92-93 21.734636510979204 26.54722184375478 28.18407067681295 23.534070968453065 94-95 21.272852630445445 26.473919884181434 28.371495830292297 23.88173165508082 96-97 21.448635898098562 26.32745983431205 28.27816384059115 23.94574042699823 98-99 21.276785079995364 26.46440309138428 28.118405850747607 24.140405977872746 100 21.50125028851085 26.164812962646415 28.044631393153395 24.289305355689343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 342.0 1 251.5 2 615.5 3 1009.0 4 617.0 5 485.0 6 1118.5 7 2836.0 8 5309.5 9 6393.0 10 4490.0 11 2416.5 12 2270.5 13 2575.0 14 2917.0 15 3358.0 16 3943.0 17 4595.5 18 5325.5 19 5950.0 20 6821.5 21 8119.5 22 9609.0 23 11861.0 24 14834.0 25 18211.0 26 22426.5 27 27517.0 28 32728.5 29 38755.5 30 46021.5 31 53620.5 32 63987.5 33 71154.0 34 74694.5 35 80173.5 36 86675.0 37 97041.5 38 103149.5 39 102425.0 40 102011.5 41 102158.5 42 101944.0 43 103194.0 44 106066.5 45 113599.0 46 121050.0 47 124144.0 48 125557.5 49 123315.0 50 119923.5 51 117885.0 52 115731.5 53 113387.0 54 110841.5 55 102419.0 56 92458.5 57 81789.0 58 70939.5 59 62031.5 60 50505.0 61 40306.5 62 32427.5 63 23769.5 64 15870.5 65 10955.5 66 8211.0 67 6219.5 68 4512.0 69 3215.0 70 2486.5 71 1875.5 72 1372.5 73 1148.0 74 905.5 75 750.0 76 574.0 77 374.5 78 273.0 79 166.0 80 114.0 81 85.5 82 49.5 83 31.5 84 24.5 85 10.5 86 4.5 87 3.0 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002781796759535805 2 0.0033800326218015697 3 2.3929434490630578E-4 4 0.050431283189003945 5 0.0029612675182155344 6 0.0050251812430324215 7 0.0 8 3.888533104727469E-4 9 0.0 10-11 1.1964717245315289E-4 12-13 0.0 14-15 0.0 16-17 2.8416203457623816E-4 18-19 1.9442665523637344E-4 20-21 1.046912758965088E-4 22-23 0.0 24-25 0.0 26-27 8.973537933986468E-5 28-29 0.0 30-31 1.9442665523637344E-4 32-33 4.3372100014267925E-4 34-35 1.34603069009797E-4 36-37 5.9823586226576444E-5 38-39 6.580594484923409E-4 40-41 3.1407382768952635E-4 42-43 0.0 44-45 5.23456379482544E-4 46-47 0.0 48-49 5.9823586226576444E-5 50-51 0.0 52-53 4.03809207029391E-4 54-55 2.991179311328822E-4 56-57 8.973537933986468E-5 58-59 9.123096899552909E-4 60-61 4.935445863692557E-4 62-63 1.7947075867972935E-4 64-65 2.991179311328822E-4 66-67 0.001196471724531529 68-69 0.001974178345477023 70-71 0.002811708552649093 72-73 0.0026172818974127196 74-75 0.002422855242176346 76-77 0.0033501208286882815 78-79 0.001076824552078376 80-81 9.721332761818673E-4 82-83 5.085004829258998E-4 84-85 4.6363279325596743E-4 86-87 1.4955896556644111E-5 88-89 0.0 90-91 4.486768966993234E-5 92-93 4.486768966993234E-5 94-95 8.973537933986468E-5 96-97 3.2902972424617047E-4 98-99 1.4955896556644111E-5 100 0.08177884237173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3343163.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.29029643490025 #Duplication Level Percentage of deduplicated Percentage of total 1 67.48027183159003 34.61083145749902 2 16.17953097657057 16.597058799319115 3 6.719103480249691 10.338744278363299 4 3.3674885614669274 6.908779462350981 5 2.014761779751189 5.166886446457284 6 1.1703926014372696 3.601787008375898 7 0.7724634935752005 2.7733917099427488 8 0.524770198333958 2.1532495226200083 9 0.3771986250264577 1.741196636319942 >10 1.3482293707805517 10.742632847783975 >50 0.030464990165560514 1.0497535191310607 >100 0.013218186900941046 1.2262377138238663 >500 0.0010529520758394521 0.40195359701762 >1k 7.657833278832369E-4 0.5832672202967292 >5k 9.572291598540461E-5 0.46008393277783244 >10k+ 1.9144583197080922E-4 1.6441458479206115 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17577 0.5257595875522671 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15911 0.47592654022552894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9371 0.28030341326462394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.973537933986468E-5 2 0.0 0.0 0.0 0.0 8.973537933986468E-5 3 0.0 0.0 0.0 0.0 8.973537933986468E-5 4 0.0 0.0 0.0 0.0 8.973537933986468E-5 5 0.0 0.0 0.0 2.9911793113288225E-5 8.973537933986468E-5 6 0.0 0.0 0.0 2.9911793113288225E-5 8.973537933986468E-5 7 0.0 0.0 0.0 2.9911793113288225E-5 1.196471724531529E-4 8 0.0 0.0 0.0 2.9911793113288225E-5 1.196471724531529E-4 9 0.0 0.0 0.0 2.9911793113288225E-5 1.196471724531529E-4 10-11 0.0 0.0 0.0 2.9911793113288225E-5 1.3460306900979702E-4 12-13 0.0 0.0 0.0 2.9911793113288225E-5 4.785886898126116E-4 14-15 0.0 0.0 0.0 2.9911793113288225E-5 6.131917588224087E-4 16-17 0.0 0.0 0.0 2.9911793113288225E-5 7.627507243888498E-4 18-19 0.0 0.0 0.0 7.477948278322057E-5 8.07618414058782E-4 20-21 0.0 0.0 0.0 2.2433844834966168E-4 8.225743106154261E-4 22-23 0.0 0.0 0.0 5.832799657091204E-4 8.674420002853585E-4 24-25 0.0 0.0 0.0 0.0012114276210881732 8.674420002853585E-4 26-27 0.0 0.0 0.0 0.0024527670352896347 9.123096899552909E-4 28-29 0.0 0.0 0.0 0.006804932933273071 9.571773796252232E-4 30-31 0.0 0.0 0.0 0.018904253247598157 9.870891727385115E-4 32-33 0.0 0.0 0.0 0.044942469152715554 0.0010170009658517997 34-35 0.0 0.0 0.0 0.08429143299324621 0.0010768245520783762 36-37 0.0 0.0 0.0 0.1435167833575569 0.0010768245520783762 38-39 0.0 0.0 0.0 0.23313251552496844 0.0011067363451916644 40-41 0.0 0.0 0.0 0.3319162122816028 0.0011067363451916644 42-43 0.0 0.0 0.0 0.43603916410895904 0.0011665599314182409 44-45 0.0 0.0 0.0 0.564719698082325 0.0013161188969846819 46-47 0.0 0.0 0.0 0.72272874520327 0.0014208101728811907 48-49 0.0 0.0 0.0 0.8948412027771305 0.0014955896556644113 50-51 0.0 0.0 0.0 1.088041474495859 0.0014955896556644113 52-53 0.0 0.0 0.0 1.2744069014882013 0.0016451486212308523 54-55 0.0 0.0 0.0 1.4780164772103543 0.0016750604143441405 56-57 0.0 0.0 0.0 1.7201225306693093 0.0017049722074574288 58-59 0.0 0.0 0.0 1.994308982242266 0.001734884000570717 60-61 0.0 0.0 0.0 2.2936662077200545 0.0017647957936840052 62-63 0.0 0.0 0.0 2.598392600061678 0.001974178345477023 64-65 0.0 0.0 0.0 2.914246179441445 0.0020639137248168876 66-67 0.0 0.0 0.0 3.273591506007933 0.0020639137248168876 68-69 0.0 0.0 0.0 3.6862097361091877 0.0020639137248168876 70-71 0.0 0.0 0.0 4.134722117946388 0.002168605000713396 72-73 0.0 0.0 0.0 4.59312034740753 0.002288252173166549 74-75 0.0 0.0 0.0 5.045356747487334 0.0023630316559497696 76-77 0.0 0.0 0.0 5.532993754716716 0.002392943449063058 78-79 0.0 0.0 0.0 6.076281653033369 0.0024527670352896343 80-81 0.0 0.0 0.0 6.672034836470732 0.002557458311186143 82-83 0.0 0.0 0.0 7.293422426606181 0.002677105483639296 84-85 0.0 0.0 0.0 7.895187880459314 0.0027518849664225167 86-87 0.0 0.0 0.0 8.52336544763148 0.002901443931988958 88 0.0 0.0 0.0 9.007697201721843 0.003051002897555399 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8400 0.0 61.253937 1 GTATCAA 15430 0.0 41.820015 1 ATCAACG 19570 0.0 32.53998 3 CAACGCA 19725 0.0 32.28783 5 TATCAAC 20165 0.0 31.813549 2 TCAACGC 20295 0.0 31.354382 4 AACGCAG 20710 0.0 31.045351 6 ACGCAGA 22760 0.0 27.959154 7 CGCAGAG 22945 0.0 27.569862 8 GTGGTAT 2735 0.0 27.51431 1 GTACTAG 1165 0.0 27.452318 1 GCAGAGT 24780 0.0 25.33861 9 TGGTATC 2985 0.0 24.579681 2 GTACATG 10375 0.0 24.026125 1 TACATGG 10505 0.0 23.191547 2 GAGTACT 18780 0.0 23.123524 12-13 ACATGGG 10010 0.0 22.881495 3 CAGAGTA 24285 0.0 22.623198 10-11 AGAGTAC 23530 0.0 22.130716 10-11 CATGGGG 6775 0.0 21.867079 4 >>END_MODULE