FastQCFastQC Report
Mon 16 Jan 2017
ERR1146512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200210
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT289190.6885131933879496No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77260.1839431837931913No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA58090.13830260867908986No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG48600.11570850028927125No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA47180.11232771694748594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG136900.025.491781
TACATGG135400.024.8642232
ACATGGG139350.023.2146433
GTACTAG15950.023.0003051
CTAGTAC20850.021.1983643
TGATCGC10800.020.4503619
CATGGGG103450.020.407084
CTGTGCG20850.019.6083059
TACGGGC12000.019.1990554
GTGATCG11050.019.1385128
GTATAAG21200.018.8574391
AGAGTAC95950.018.80784410-11
ATACGGG13150.018.2358063
GCTATTG25250.018.0537858
GTATATG19300.018.0332551
GGCGTCG13050.018.0060088
TAGTACT23850.017.7426784
GTCCTAC14900.017.676711
ATGGGGG71750.017.6270585
GAGTACA105450.017.394731
Produced by FastQC (version 0.11.5)