##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146512_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4200210 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.205177122096277 31.0 31.0 34.0 28.0 34.0 2 31.370870027927175 31.0 31.0 34.0 28.0 34.0 3 31.48006075886682 33.0 31.0 34.0 28.0 34.0 4 34.92523897614643 37.0 35.0 37.0 32.0 37.0 5 34.99982381833289 37.0 35.0 37.0 32.0 37.0 6 35.17013744550868 37.0 35.0 37.0 32.0 37.0 7 35.12395475464322 37.0 35.0 37.0 32.0 37.0 8 35.08123593820309 37.0 35.0 37.0 32.0 37.0 9 36.69786796374467 39.0 37.0 39.0 33.0 39.0 10-11 36.68444053987777 39.0 37.0 39.0 32.5 39.0 12-13 36.63173507991267 39.0 37.0 39.0 32.0 39.0 14-15 37.983120248749465 40.0 38.0 41.0 33.0 41.0 16-17 38.03040931286769 40.0 38.0 41.0 33.0 41.0 18-19 38.066020627540055 40.0 38.0 41.0 32.5 41.0 20-21 38.15938953052348 40.0 38.0 41.0 33.0 41.0 22-23 38.1829457336657 40.0 38.0 41.0 33.0 41.0 24-25 38.2101054471086 40.0 38.0 41.0 32.5 41.0 26-27 38.2668532049588 40.0 38.0 41.0 33.0 41.0 28-29 38.26266722378167 40.0 38.0 41.0 33.0 41.0 30-31 38.23944338497361 40.0 38.0 41.0 33.0 41.0 32-33 38.19965358874913 40.0 38.0 41.0 32.5 41.0 34-35 38.14083010135208 40.0 38.0 41.0 32.0 41.0 36-37 38.055099983096085 40.0 38.0 41.0 32.0 41.0 38-39 37.99061963568488 40.0 38.0 41.0 32.0 41.0 40-41 37.95278557500696 40.0 38.0 41.0 32.0 41.0 42-43 37.79433230719417 40.0 38.0 41.0 31.0 41.0 44-45 37.673200625683 40.0 37.0 41.0 31.0 41.0 46-47 37.557945317019865 40.0 37.0 41.0 31.0 41.0 48-49 37.434287809419054 40.0 37.0 41.0 31.0 41.0 50-51 37.28684827663379 40.0 36.0 41.0 30.0 41.0 52-53 37.1774368424436 40.0 36.0 41.0 30.0 41.0 54-55 37.05260272700651 39.5 35.5 41.0 30.0 41.0 56-57 36.838988645805806 39.0 35.0 41.0 30.0 41.0 58-59 36.63349808700041 39.0 35.0 41.0 30.0 41.0 60-61 36.378215970153875 39.0 35.0 41.0 30.0 41.0 62-63 36.16085695715214 38.0 35.0 40.0 30.0 41.0 64-65 35.86340480595018 37.0 35.0 40.0 29.0 41.0 66-67 35.585790353339476 37.0 35.0 40.0 29.0 41.0 68-69 35.29932705745665 36.5 34.0 39.5 29.0 41.0 70-71 34.98789227205306 36.0 34.0 39.0 28.5 41.0 72-73 34.6317504362877 35.0 34.0 39.0 28.0 41.0 74-75 34.316084552915214 35.0 34.0 37.5 28.0 40.0 76-77 34.043543656150526 35.0 34.0 37.0 28.0 39.0 78-79 33.71914106675619 35.0 34.0 37.0 27.0 39.0 80-81 33.468059216086814 35.0 33.5 36.0 27.0 39.0 82-83 33.18849628947124 35.0 33.0 36.0 26.5 37.0 84-85 32.99526856990484 35.0 33.0 35.5 26.5 37.0 86-87 32.81923844283976 35.0 33.0 35.0 26.0 37.0 88-89 32.62834846352921 35.0 33.0 35.0 26.0 36.0 90-91 32.46261115515652 35.0 33.0 35.0 25.5 36.0 92-93 32.30550460572209 35.0 33.0 35.0 25.0 36.0 94-95 32.17217948626378 35.0 33.0 35.0 25.0 36.0 96-97 32.06600408074834 35.0 33.0 35.0 25.0 35.0 98-99 31.965039962287598 35.0 33.0 35.0 25.0 35.0 100 30.783353213291715 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3055623246278145 1101 2 0.30963516093840937 1101 3 0.30841018083809146 1101 4 0.33472205886639017 1101 5 0.23786483820972393 1101 6 0.313559859730411 1101 7 0.39343233508784436 1101 8 0.4059166746551739 1101 9 0.5220093669187591 1101 10-11 0.5200724653306068 1101 12-13 0.4945120818547295 1101 14-15 0.5877350086708475 1101 16-17 0.5688113913418746 1101 18-19 0.522028718294699 1101 20-21 0.530154270182635 1101 22-23 0.5444997562612031 1101 24-25 0.5723178216631908 1101 26-27 0.5981118029142465 1101 28-29 0.4996886987125464 1101 30-31 0.5763516474723502 1101 32-33 0.5649539741112335 1101 34-35 0.5326796349563807 1101 36-37 0.5247957912050296 1101 38-39 0.5067252133425555 1101 40-41 0.5336353148001862 1101 42-43 0.5823192085778075 1101 44-45 0.5460892196779099 1101 46-47 0.4687371591063254 1101 48-49 0.4724047160012077 1101 50-51 0.48601619559336484 1101 52-53 0.46401893509920455 1101 54-55 0.4845524300190718 1101 56-57 0.4951581882476077 1101 58-59 0.5133045272258911 1101 60-61 0.4695548556960034 1101 62-63 0.51226310179986 1101 64-65 0.520608737633701 1101 66-67 0.4569459698206515 1101 68-69 0.4363902741857544 1101 70-71 0.41118249863311007 1101 72-73 0.42837436135418017 1101 74-75 0.47034568972158297 1101 76-77 0.4749787854509435 1101 78-79 0.4553007936940432 1101 80-81 0.43393635342228265 1101 82-83 0.45235868359376497 1101 84-85 0.4655421908710835 1101 86-87 0.394054596368278 1101 88-89 0.429988442796855 1101 90-91 0.4319593996089921 1101 92-93 0.3977957644829857 1101 94-95 0.4423232402199062 1101 96-97 0.42868299745779836 1101 98-99 0.4467899834719731 1101 100 0.5556127381888913 1102 1 0.23659378809878007 1102 2 0.26606800158391053 1102 3 0.2487255441788001 1102 4 0.27502341271237185 1102 5 0.25844564439159257 1102 6 0.3759707509313728 1102 7 0.30493882069415434 1102 8 0.30102504668115415 1102 9 0.46074526273976346 1102 10-11 0.4808438885435038 1102 12-13 0.44424483014057614 1102 14-15 0.5670764291910331 1102 16-17 0.5338765014987459 1102 18-19 0.4954753005376773 1102 20-21 0.5538708510995178 1102 22-23 0.5209177076981177 1102 24-25 0.500709868857605 1102 26-27 0.47321892576027835 1102 28-29 0.4923591405259984 1102 30-31 0.5295053049153395 1102 32-33 0.5443589524126509 1102 34-35 0.505182207312501 1102 36-37 0.5466174506118193 1102 38-39 0.45528006528726195 1102 40-41 0.467991251972542 1102 42-43 0.5003190778225246 1102 44-45 0.4385484752257156 1102 46-47 0.5070942457439926 1102 48-49 0.44351758425089827 1102 50-51 0.4741566260817933 1102 52-53 0.4566811703704019 1102 54-55 0.45382571082915746 1102 56-57 0.4779716113763115 1102 58-59 0.4891287061441645 1102 60-61 0.5292287792122679 1102 62-63 0.4155798702791529 1102 64-65 0.46773034615993225 1102 66-67 0.4781656329980706 1102 68-69 0.48227205616326785 1102 70-71 0.43849036888568804 1102 72-73 0.46871070116628033 1102 74-75 0.5314132145934138 1102 76-77 0.47131854742551127 1102 78-79 0.39569331226675786 1102 80-81 0.35703075138930984 1102 82-83 0.4052506244465022 1102 84-85 0.38347228733812955 1102 86-87 0.3728768414955681 1102 88-89 0.3725463038986234 1102 90-91 0.3783883450655665 1102 92-93 0.28012196622808716 1102 94-95 0.2779681745205309 1102 96-97 0.33041892493750424 1102 98-99 0.35909698465516016 1102 100 0.2922184766015441 1103 1 0.22828962495929872 1103 2 0.2653295383684373 1103 3 0.3034713244328273 1103 4 0.282402034449305 1103 5 0.3245959249128987 1103 6 0.33882117677576673 1103 7 0.3918122350334414 1103 8 0.4160976419861626 1103 9 0.5379589287179343 1103 10-11 0.49439975649393375 1103 12-13 0.5279315429980898 1103 14-15 0.6383612743127927 1103 16-17 0.5849142945337533 1103 18-19 0.5789108817767001 1103 20-21 0.5122444754730182 1103 22-23 0.5007434886582871 1103 24-25 0.5315055031041069 1103 26-27 0.5363499940597976 1103 28-29 0.49555337863112214 1103 30-31 0.5385667806957031 1103 32-33 0.49617005020539295 1103 34-35 0.46017207661171255 1103 36-37 0.5121548478875653 1103 38-39 0.5398653198695271 1103 40-41 0.46534946881313033 1103 42-43 0.5306723484668865 1103 44-45 0.5471378279688679 1103 46-47 0.5435525274498829 1103 48-49 0.47441632391034716 1103 50-51 0.48911353500511723 1103 52-53 0.5102202412485539 1103 54-55 0.4176155083306341 1103 56-57 0.49828814515138475 1103 58-59 0.48256699773546075 1103 60-61 0.48590699374811663 1103 62-63 0.44892669086412695 1103 64-65 0.45925993675262333 1103 66-67 0.46203914712287997 1103 68-69 0.44970105149487694 1103 70-71 0.43565296393485653 1103 72-73 0.4106947501468454 1103 74-75 0.44732224607059834 1103 76-77 0.4166112527261987 1103 78-79 0.416704505584228 1103 80-81 0.3663033470278876 1103 82-83 0.42723018732675655 1103 84-85 0.4557963683098549 1103 86-87 0.4490853988389034 1103 88-89 0.4153039597312116 1103 90-91 0.4429233336742513 1103 92-93 0.4035150409114223 1103 94-95 0.4374297348621212 1103 96-97 0.47438363000205186 1103 98-99 0.4267625984917345 1103 100 0.5473042891614099 1104 1 0.11534128365939011 1104 2 0.06266416572187339 1104 3 0.06599082859295535 1104 4 0.13793265462107485 1104 5 0.07969060276399631 1104 6 0.2414786196895804 1104 7 0.2112856411308499 1104 8 0.22819815099781238 1104 9 0.28814217221020044 1104 10-11 0.296037141043513 1104 12-13 0.3425158828987094 1104 14-15 0.4349365244941126 1104 16-17 0.4215984662608818 1104 18-19 0.37109543718360527 1104 20-21 0.39845171412603975 1104 22-23 0.358310050168285 1104 24-25 0.41357857910007567 1104 26-27 0.38250975782393937 1104 28-29 0.34542423977553227 1104 30-31 0.3677763459884531 1104 32-33 0.3499461292893855 1104 34-35 0.33632745797045516 1104 36-37 0.3778672361300863 1104 38-39 0.3349529995560445 1104 40-41 0.3394927243473589 1104 42-43 0.41370023796851285 1104 44-45 0.33576137197695033 1104 46-47 0.35502256130563126 1104 48-49 0.3105403705441958 1104 50-51 0.2850479592731858 1104 52-53 0.266753060342225 1104 54-55 0.27719366935543377 1104 56-57 0.28655914938186555 1104 58-59 0.3191926613681275 1104 60-61 0.35134508692107147 1104 62-63 0.3273781859871647 1104 64-65 0.35001343855417844 1104 66-67 0.3003986064694928 1104 68-69 0.2958325261128891 1104 70-71 0.2617362496371669 1104 72-73 0.3441817922267134 1104 74-75 0.3266924147668888 1104 76-77 0.26862809779430563 1104 78-79 0.32852355294262736 1104 80-81 0.2927698563630443 1104 82-83 0.29544798515576787 1104 84-85 0.25398708224194166 1104 86-87 0.27907942873260083 1104 88-89 0.23085056919152436 1104 90-91 0.2944932540927141 1104 92-93 0.3064018671341415 1104 94-95 0.2694132592634162 1104 96-97 0.24156935206211472 1104 98-99 0.2981538683471996 1104 100 0.29849401222777416 1105 1 0.15980020506871995 1105 2 0.1789935847435018 1105 3 0.1701860361939609 1105 4 0.2466080145465952 1105 5 0.21808581461295518 1105 6 0.2955889124797437 1105 7 0.3455881051205978 1105 8 0.36706594761998446 1105 9 0.4589241539864872 1105 10-11 0.4760080315029711 1105 12-13 0.4672601207274454 1105 14-15 0.5674157980233403 1105 16-17 0.5732209630660705 1105 18-19 0.5070542156670186 1105 20-21 0.49397921696686353 1105 22-23 0.5042792294991614 1105 24-25 0.5460136584305957 1105 26-27 0.5519994326431359 1105 28-29 0.4613174139345304 1105 30-31 0.4976719459056227 1105 32-33 0.4937351352329813 1105 34-35 0.4955220416533592 1105 36-37 0.5145036405564554 1105 38-39 0.5283415584910713 1105 40-41 0.517058100786798 1105 42-43 0.5268464578213212 1105 44-45 0.5009832921225126 1105 46-47 0.5159878997789917 1105 48-49 0.4983264989421201 1105 50-51 0.5145317843070814 1105 52-53 0.5241697761258521 1105 54-55 0.44644345651528994 1105 56-57 0.49590197998765717 1105 58-59 0.4713813859733378 1105 60-61 0.4958684688049715 1105 62-63 0.4760848059794469 1105 64-65 0.5281074307096247 1105 66-67 0.4702369121203276 1105 68-69 0.49005247804021934 1105 70-71 0.4832157253866711 1105 72-73 0.45952594058292817 1105 74-75 0.4927200631885995 1105 76-77 0.46847337938049805 1105 78-79 0.4427678797128678 1105 80-81 0.4151011130668394 1105 82-83 0.4461009198718031 1105 84-85 0.4459497560097674 1105 86-87 0.39137135647138166 1105 88-89 0.37524385751424916 1105 90-91 0.38189864863905143 1105 92-93 0.4376553739078517 1105 94-95 0.4623725426337444 1105 96-97 0.4465901117200133 1105 98-99 0.3679177020717077 1105 100 0.4672743673920472 1106 1 0.0822630290351789 1106 2 0.12640926656661833 1106 3 0.12154903069766476 1106 4 0.08511649252882592 1106 5 0.08690628342744589 1106 6 0.16273588951695928 1106 7 0.17121059322644072 1106 8 0.22243461221557936 1106 9 0.25797308296913 1106 10-11 0.26816987623803357 1106 12-13 0.26578339851849364 1106 14-15 0.2886038560711839 1106 16-17 0.2336334568202787 1106 18-19 0.26318268072316897 1106 20-21 0.2588062628707064 1106 22-23 0.2950225872363319 1106 24-25 0.33074879616386 1106 26-27 0.2755281144010837 1106 28-29 0.2495469087315172 1106 30-31 0.21970748988685074 1106 32-33 0.29101767308790727 1106 34-35 0.23553374572235697 1106 36-37 0.2789577312779272 1106 38-39 0.2823312746606135 1106 40-41 0.22522011388666385 1106 42-43 0.22320689969744478 1106 44-45 0.2133019469420887 1106 46-47 0.30087140220126685 1106 48-49 0.25756604674685946 1106 50-51 0.2930871328765221 1106 52-53 0.20123318176552374 1106 54-55 0.19050687301721325 1106 56-57 0.1878936133434408 1106 58-59 0.19397193636329035 1106 60-61 0.24226949474400783 1106 62-63 0.20454648126349895 1106 64-65 0.2662477872085063 1106 66-67 0.25822876815213647 1106 68-69 0.2095020880856282 1106 70-71 0.18286369360373556 1106 72-73 0.15496864495606388 1106 74-75 0.20484166330591336 1106 76-77 0.1562248675080582 1106 78-79 0.19174608190269993 1106 80-81 0.1334736093193598 1106 82-83 0.11724508087200292 1106 84-85 0.13795403030317033 1106 86-87 0.09091936487875074 1106 88-89 0.1293986798079203 1106 90-91 0.09428720200526186 1106 92-93 0.1341100864928535 1106 94-95 0.0982904023369855 1106 96-97 0.18137752400738805 1106 98-99 0.16487943709809372 1106 100 0.2475733778415261 1107 1 0.07968610525075803 1107 2 0.15091926422783075 1107 3 0.04765421308369966 1107 4 -0.008703746658227374 1107 5 0.01905791571743265 1107 6 0.055754029576192465 1107 7 0.08064775998201412 1107 8 0.1466430376332113 1107 9 0.14509421904190845 1107 10-11 0.16428625327166912 1107 12-13 0.10808753817303796 1107 14-15 0.2009723518101154 1107 16-17 0.15031035458897435 1107 18-19 0.21208723452711808 1107 20-21 0.21850674035133721 1107 22-23 0.2292221567942505 1107 24-25 0.2746612860794784 1107 26-27 0.251551741414346 1107 28-29 0.2718854075450352 1107 30-31 0.29078105921877295 1107 32-33 0.2630994439445047 1107 34-35 0.24691808477827237 1107 36-37 0.230928041811147 1107 38-39 0.22682168168854133 1107 40-41 0.23466649126191186 1107 42-43 0.23417228038115923 1107 44-45 0.2148492653509777 1107 46-47 0.2319322395924388 1107 48-49 0.27203234323683034 1107 50-51 0.2671407778380086 1107 52-53 0.24164221999814117 1107 54-55 0.19684046825974377 1107 56-57 0.20664496340216942 1107 58-59 0.20440562384341376 1107 60-61 0.27955685556642607 1107 62-63 0.22076035158954 1107 64-65 0.2629428565706462 1107 66-67 0.23932492386811077 1107 68-69 0.2564257542538755 1107 70-71 0.1582131453025113 1107 72-73 0.20627270314721358 1107 74-75 0.22484417290414171 1107 76-77 0.18221470035042842 1107 78-79 0.1974283509999495 1107 80-81 0.15995156558665968 1107 82-83 0.1505037350441114 1107 84-85 0.18857848666638688 1107 86-87 0.208891070801144 1107 88-89 0.1820004762072429 1107 90-91 0.23522905027837737 1107 92-93 0.18210026621162 1107 94-95 0.20464969796766042 1107 96-97 0.16897331576591768 1107 98-99 0.2507318858615619 1107 100 0.3253114020650614 1108 1 0.03877225120302796 1108 2 0.060666525391724235 1108 3 0.07959050378811838 1108 4 0.0736047913357254 1108 5 0.05352496409473417 1108 6 0.09225741501924034 1108 7 0.10045149436342626 1108 8 0.13225471966371316 1108 9 0.1155043358174197 1108 10-11 0.1773330129063453 1108 12-13 0.1371964558386054 1108 14-15 0.11858837428377456 1108 16-17 0.13798294055600024 1108 18-19 0.11043906881894117 1108 20-21 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-0.3268314872117024 2215 4 -0.2906409998804591 2215 5 -0.2978341709627088 2215 6 -0.35816470377312726 2215 7 -0.39329903457589666 2215 8 -0.36276268198624706 2215 9 -0.5380280430142292 2215 10-11 -0.5187254825187466 2215 12-13 -0.48227637529637235 2215 14-15 -0.6533110247443403 2215 16-17 -0.649600371673543 2215 18-19 -0.7080522909445932 2215 20-21 -0.5871006801919236 2215 22-23 -0.5278347566892663 2215 24-25 -0.5291447941114242 2215 26-27 -0.596880630449796 2215 28-29 -0.5689038434640423 2215 30-31 -0.6266573781431717 2215 32-33 -0.5904095108148297 2215 34-35 -0.6254989787061049 2215 36-37 -0.5921440455839715 2215 38-39 -0.6690277700585412 2215 40-41 -0.6174612951396838 2215 42-43 -0.674730831703954 2215 44-45 -0.6028607812897917 2215 46-47 -0.5729683622017632 2215 48-49 -0.5915750471330767 2215 50-51 -0.5604362387989852 2215 52-53 -0.5942920336002757 2215 54-55 -0.5904874411966077 2215 56-57 -0.6231787783612432 2215 58-59 -0.57115910961938 2215 60-61 -0.5250704232998658 2215 62-63 -0.5755192834704488 2215 64-65 -0.5900019468746294 2215 66-67 -0.5878627910554641 2215 68-69 -0.5501096195626829 2215 70-71 -0.5002459758857682 2215 72-73 -0.42855937892165485 2215 74-75 -0.3705857053956265 2215 76-77 -0.43298212003521286 2215 78-79 -0.41733452260424286 2215 80-81 -0.4483900435511359 2215 82-83 -0.48117744653086447 2215 84-85 -0.46273553780485344 2215 86-87 -0.42176983363168574 2215 88-89 -0.3847978107340282 2215 90-91 -0.4791231952906543 2215 92-93 -0.4621354036644618 2215 94-95 -0.48550528970226736 2215 96-97 -0.4789789227845276 2215 98-99 -0.48235337285353097 2215 100 -0.4346867973735158 2216 1 -0.14746375947844825 2216 2 -0.16403885222567638 2216 3 -0.15845704399912464 2216 4 -0.14008073029565082 2216 5 -0.17179776793571477 2216 6 -0.3292977051548931 2216 7 -0.30751106276787965 2216 8 -0.3422731978662199 2216 9 -0.4122891980595327 2216 10-11 -0.5381433523134405 2216 12-13 -0.41595604294910515 2216 14-15 -0.6028374756462327 2216 16-17 -0.5515381683509446 2216 18-19 -0.571004244091462 2216 20-21 -0.5751509724628434 2216 22-23 -0.6075708586853779 2216 24-25 -0.6327493399632971 2216 26-27 -0.7501587010090631 2216 28-29 -0.7083791318350663 2216 30-31 -0.7293077897740048 2216 32-33 -0.6887929022798005 2216 34-35 -0.7154426318999754 2216 36-37 -0.6598336438515844 2216 38-39 -0.6390501893213312 2216 40-41 -0.6688979410513767 2216 42-43 -0.6635203274332611 2216 44-45 -0.6049011817265821 2216 46-47 -0.6268167560365896 2216 48-49 -0.5643130228254094 2216 50-51 -0.6743904591856023 2216 52-53 -0.5995963140846001 2216 54-55 -0.6486327980499453 2216 56-57 -0.5895390978996531 2216 58-59 -0.6234580972173944 2216 60-61 -0.6636924105126951 2216 62-63 -0.6283350931220681 2216 64-65 -0.5787342534699818 2216 66-67 -0.5669583934557778 2216 68-69 -0.6663107073439676 2216 70-71 -0.6164048849272064 2216 72-73 -0.5796325581935875 2216 74-75 -0.5043359546336319 2216 76-77 -0.5441400738980917 2216 78-79 -0.4959206850097857 2216 80-81 -0.5788241647238905 2216 82-83 -0.5195948278876514 2216 84-85 -0.5247883356113832 2216 86-87 -0.5481719883698588 2216 88-89 -0.5120879323796359 2216 90-91 -0.4748970601338911 2216 92-93 -0.41100046199831297 2216 94-95 -0.47764353733368026 2216 96-97 -0.44880344800404615 2216 98-99 -0.5056283995605 2216 100 -0.5172702137030569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1310.0 1 343.0 2 222.0 3 221.0 4 184.0 5 227.0 6 326.0 7 766.0 8 2195.0 9 2910.0 10 3038.0 11 3385.0 12 4213.0 13 5644.0 14 8585.0 15 10616.0 16 11704.0 17 12563.0 18 14337.0 19 15792.0 20 18175.0 21 19868.0 22 22258.0 23 25903.0 24 31095.0 25 37443.0 26 39396.0 27 36484.0 28 40143.0 29 50297.0 30 57669.0 31 67111.0 32 85168.0 33 116219.0 34 173916.0 35 270823.0 36 495544.0 37 1004788.0 38 1144690.0 39 363782.0 40 857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09247795857065 20.074133178517823 13.129075048820745 23.704313814090785 2 15.022385307462056 21.533207731650766 39.39542516360143 24.048981797285744 3 19.08298214754616 26.30593218440816 30.390346397937062 24.220739270108613 4 12.168892457885901 17.765781393902497 39.771769524869335 30.293556623342273 5 12.916333956933244 39.17019988319118 35.00571057386681 12.907755586008774 6 29.289720285419733 38.75276857237228 18.165528575098552 13.791982567109432 7 25.625716389563408 33.4914771982585 23.319353062989855 17.563453349188233 8 24.8395671906594 34.69445142810162 20.927530291530285 19.538451089708683 9 25.316598355284945 16.737122458695083 21.484060598176107 36.462218587843864 10-11 24.384156604407288 26.50672373422363 28.524886496141516 20.584233165227566 12-13 25.429449486233136 24.809519742078347 27.679973867598935 22.081056904089586 14-15 22.513232660232035 26.184267541413607 26.18617379269065 25.11632600566371 16-17 21.807136630229223 28.837694019591126 26.757937204354576 22.597232145825075 18-19 21.627203287761716 29.276772724559525 27.447386960256082 21.648637027422676 20-21 23.263068533257343 27.609024767049444 26.544021150204383 22.583885549488826 22-23 22.939008104779674 27.495538612402726 26.577313026489307 22.988140256328297 24-25 23.26497938825882 27.667174558650444 26.66450785767158 22.403338195419156 26-27 22.825687863546896 27.405797975085637 27.272405615702773 22.496108545664693 28-29 22.10125769693319 28.911016649275766 26.5640790165654 22.423646637225648 30-31 22.703714431449487 27.74263238520404 26.55190158649327 23.0017515968532 32-33 22.886012579626506 28.032652362859157 26.348489897920558 22.73284515959378 34-35 23.38387465916419 27.806852319018034 26.6222616961315 22.18701132568628 36-37 22.99616092195066 27.40822911503413 27.20332338811149 22.392286574903725 38-39 22.712081750678163 27.9673786559543 27.06754401705705 22.252995576310493 40-41 22.629352508865214 27.844070441293482 26.93329066243707 22.593286387404238 42-43 22.403436902736274 27.867461667882047 26.279294221942607 23.44980720743908 44-45 22.546915324838732 27.767116497751637 26.57546128934632 23.110506888063313 46-47 23.06695703168097 27.87380226218779 26.5758692185046 22.483371487626645 48-49 22.955793596766366 27.552536011918555 26.799070718591622 22.692599672723453 50-51 21.998521797059155 27.835516977231073 27.324818474510295 22.841142751199474 52-53 22.43538406010465 28.255957373563884 26.258760703289486 23.04989786304198 54-55 22.088733349569324 27.628717498030475 26.675051555628844 23.607497596771356 56-57 22.979343528014123 27.193618901169348 26.517283433534487 23.30975413728204 58-59 23.23817215999451 27.21106852791334 26.733085968938287 22.817673343153867 60-61 22.548659089663325 27.65276940345994 27.25783657409504 22.5407349327817 62-63 22.3407318044489 27.616584250029252 26.86492020771424 23.17776373780761 64-65 21.864447214892927 28.33333055282205 26.51641339783802 23.285808834447007 66-67 22.616155697546702 27.32445755596398 26.25921412154507 23.800172624944242 68-69 22.012512589568853 27.396284116957037 26.74747743887297 23.843725854601143 70-71 22.767903851502545 27.382316259211798 26.36818155493425 23.481598334351407 72-73 22.704900109688385 27.072869194365317 27.357653848239178 22.86457684770712 74-75 22.179869229391485 28.400666806408903 26.566818099106005 22.85264586509361 76-77 22.336649737288994 27.445301260581456 26.53965641478274 23.678392587346806 78-79 22.55891417282889 27.48543524216759 26.654206961791576 23.301443623211945 80-81 22.798614542065067 27.4026667658118 26.762846981161708 23.035871710961427 82-83 22.821488646296796 26.84281307518547 27.59567481378068 22.740023464737053 84-85 22.766467682394502 27.49637793331393 26.833466442347593 22.90368794194397 86-87 22.27755978393049 27.774913252960076 26.778956304325263 23.16857065878417 88-89 22.5043171801128 27.33760467642479 26.30019451738383 23.85788362607858 90-91 22.794093464536715 26.802394167820538 26.612500387270828 23.791011980371923 92-93 23.28204162754453 27.16467759385739 26.65738061988533 22.89590015871276 94-95 22.542886389776875 27.00172926540715 27.039762857218776 23.4156214875972 96-97 22.16661570969635 27.168640087542723 27.243276360819195 23.421467841941727 98-99 22.46513267435093 27.676915420846083 26.31524381030192 23.54270809450107 100 22.901719151682457 26.5403901237267 26.17562322202091 24.38226750256993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3185.0 1 2268.0 2 1436.0 3 1525.0 4 1396.5 5 1211.5 6 1107.0 7 1082.0 8 1133.0 9 1129.5 10 992.5 11 874.0 12 848.5 13 851.5 14 941.5 15 1116.0 16 1315.5 17 1569.5 18 1947.0 19 2332.5 20 3049.0 21 4451.0 22 6551.0 23 8756.0 24 11162.5 25 14531.0 26 20124.0 27 26669.0 28 33488.5 29 41780.5 30 49434.5 31 57485.5 32 67401.5 33 75276.0 34 81826.0 35 89660.5 36 98424.5 37 108823.0 38 115066.0 39 115788.0 40 115673.5 41 115391.5 42 117506.0 43 124100.0 44 131459.5 45 143352.0 46 158225.0 47 184906.5 48 227189.0 49 220600.5 50 176184.0 51 159222.5 52 155360.5 53 152346.5 54 146634.5 55 133262.0 56 121424.5 57 105770.0 58 89296.0 59 79299.0 60 66312.5 61 52977.0 62 42925.5 63 33301.0 64 23894.5 65 16242.0 66 11768.0 67 9027.5 68 6857.5 69 5085.5 70 3974.5 71 3221.0 72 2456.5 73 2051.0 74 1623.5 75 1257.0 76 962.0 77 679.5 78 519.5 79 378.5 80 275.5 81 186.0 82 137.0 83 101.5 84 75.5 85 58.0 86 44.0 87 36.0 88 27.0 89 18.5 90 17.5 91 19.0 92 15.0 93 11.5 94 7.0 95 6.5 96 8.5 97 6.5 98 6.5 99 6.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1011378002528445 2 0.06699665016749162 3 0.08275776687356108 4 0.08511479187945364 5 0.08592427521481069 6 0.09532856690498809 7 0.09094783356070292 8 0.09297154189909553 9 0.07742470019356175 10-11 0.08443625437775731 12-13 0.07431771268579428 14-15 0.08312679604115032 16-17 0.08488861271222153 18-19 0.084650529378293 20-21 0.07575811685606196 22-23 0.0801269460336507 24-25 0.08217446270543616 26-27 0.07602000852338335 28-29 0.08501955854588222 30-31 0.08936457939007811 32-33 0.0909835460607922 34-35 0.09304296689927409 36-37 0.09261441689820271 38-39 0.09378102523445257 40-41 0.08930505855659598 42-43 0.09499525023748813 44-45 0.09094783356070292 46-47 0.08697184188409626 48-49 0.09676897107525576 50-51 0.09281678773204197 52-53 0.09829270441239842 54-55 0.08980503355784591 56-57 0.09684039607543432 58-59 0.10097114191909451 60-61 0.0995188335821304 62-63 0.10125684191980876 64-65 0.10330435859159423 66-67 0.1049590377623976 68-69 0.10180443358784443 70-71 0.10397099192659416 72-73 0.10076877108525525 74-75 0.10450667942793336 76-77 0.10117351275293378 78-79 0.10836362943757573 80-81 0.10335197525837994 82-83 0.10838743777096858 84-85 0.1047804752619512 86-87 0.10107827941936236 88-89 0.09961406691570183 90-91 0.0995188335821304 92-93 0.09404291690177397 94-95 0.09331676273329191 96-97 0.09187635856302423 98-99 0.10589946693141533 100 0.24786855895300472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4200210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.640808594198084 #Duplication Level Percentage of deduplicated Percentage of total 1 67.24875883293089 34.05531524255841 2 15.603868712130934 15.803850575600373 3 6.6917941685854885 10.166336029293259 4 3.5881456142028534 7.268263810278321 5 1.995409651197959 5.05245791066657 6 1.2884371044332341 3.914849807475973 7 0.9012274762042839 3.194722168560534 8 0.6006522922610146 2.433401421124509 9 0.4469749084568784 2.0371653707016586 >10 1.6046326837375946 12.490206855660766 >50 0.02500410723976167 0.8322069986513908 >100 0.004594110215569673 0.4179318581857345 >500 0.0 0.0 >1k 2.5016920174503437E-4 0.5404919856650949 >5k 1.6677946783002291E-4 0.5715727030218813 >10k+ 8.338973391501146E-5 1.221227262555581 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28919 0.6885131933879496 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7726 0.1839431837931913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5809 0.13830260867908986 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4860 0.11570850028927125 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4718 0.11232771694748594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1904166696427083E-4 2 0.0 0.0 0.0 0.0 1.1904166696427083E-4 3 0.0 0.0 0.0 0.0 1.1904166696427083E-4 4 0.0 0.0 0.0 1.1904166696427083E-4 1.1904166696427083E-4 5 0.0 0.0 0.0 1.1904166696427083E-4 1.1904166696427083E-4 6 0.0 0.0 0.0 1.1904166696427083E-4 2.142750005356875E-4 7 0.0 0.0 0.0 1.1904166696427083E-4 2.6189166732139583E-4 8 0.0 0.0 0.0 1.1904166696427083E-4 2.8570000071425E-4 9 0.0 0.0 0.0 1.6665833374997918E-4 2.8570000071425E-4 10-11 1.1904166696427083E-5 0.0 0.0 2.142750005356875E-4 3.3331666749995836E-4 12-13 2.3808333392854166E-5 0.0 0.0 2.2617916723211457E-4 0.0012261291697319896 14-15 2.3808333392854166E-5 0.0 0.0 2.6189166732139583E-4 0.0016546791708033646 16-17 2.3808333392854166E-5 0.0 0.0 5.475916680356458E-4 0.0020713250051783124 18-19 2.3808333392854166E-5 0.0 0.0 7.499625018749063E-4 0.002118941671964021 20-21 2.3808333392854166E-5 0.0 0.0 8.5710000214275E-4 0.002523683339642542 22-23 2.3808333392854166E-5 0.0 0.0 0.0011308958361605729 0.002595108339821104 24-25 2.3808333392854166E-5 0.0 0.0 0.001726104170981927 0.0026189166732139583 26-27 2.3808333392854166E-5 0.0 0.0 0.003011754174196052 0.002868904173838927 28-29 2.3808333392854166E-5 0.0 0.0 0.008523383354641792 0.0029046166739282084 30-31 2.3808333392854166E-5 0.0 0.0 0.023451208391961353 0.0029641375074103437 32-33 2.3808333392854166E-5 0.0 0.0 0.05674716264186791 0.0030712750076781876 34-35 2.3808333392854166E-5 0.0 0.0 0.11038733777596835 0.0032379333414281667 36-37 2.3808333392854166E-5 0.0 0.0 0.19122853381140467 0.0032379333414281667 38-39 2.3808333392854166E-5 0.0 0.0 0.30955595077388987 0.0033688791750888644 40-41 2.3808333392854166E-5 1.1904166696427083E-5 0.0 0.43839474692932023 0.003404591675178146 42-43 2.3808333392854166E-5 2.3808333392854166E-5 0.0 0.5728880222655535 0.0034760166753567082 44-45 2.3808333392854166E-5 2.3808333392854166E-5 0.0 0.743236647691425 0.00371410000928525 46-47 2.3808333392854166E-5 2.3808333392854166E-5 0.0 0.9506072315431847 0.003928375009820937 48-49 2.3808333392854166E-5 2.3808333392854166E-5 0.0 1.1660607445818185 0.00408312917687449 50-51 2.3808333392854166E-5 2.3808333392854166E-5 0.0 1.3958944909897362 0.004237883343928042 52-53 2.3808333392854166E-5 2.3808333392854166E-5 0.0 1.6276448082357788 0.0045235833446422916 54-55 2.3808333392854166E-5 2.3808333392854166E-5 0.0 1.88182257553789 0.004595008344820854 56-57 2.3808333392854166E-5 2.3808333392854166E-5 0.0 2.2006280638349036 0.004678337511695844 58-59 2.3808333392854166E-5 2.3808333392854166E-5 0.0 2.5412181771863787 0.0047616666785708335 60-61 2.3808333392854166E-5 2.3808333392854166E-5 0.0 2.8970694322426738 0.004833091678749396 62-63 2.3808333392854166E-5 2.3808333392854166E-5 0.0 3.265503391497092 0.0051426000128565 64-65 2.3808333392854166E-5 2.3808333392854166E-5 0.0 3.646900988283919 0.005273545846517198 66-67 2.3808333392854166E-5 2.3808333392854166E-5 0.0 4.087831322719579 0.005380683346785042 68-69 2.3808333392854166E-5 2.3808333392854166E-5 0.0 4.582425640622731 0.005547341680535021 70-71 2.3808333392854166E-5 2.3808333392854166E-5 0.0 5.11707747945936 0.005594958347320729 72-73 2.3808333392854166E-5 2.3808333392854166E-5 0.0 5.644908230778937 0.005844945847945698 74-75 2.3808333392854166E-5 2.3808333392854166E-5 0.0 6.187202544634673 0.0060949333485706665 76-77 2.3808333392854166E-5 2.3808333392854166E-5 0.0 6.770661466926654 0.006142550015356375 78-79 2.3808333392854166E-5 2.3808333392854166E-5 0.0 7.443365926941748 0.006261591682320646 80-81 2.3808333392854166E-5 2.3808333392854166E-5 0.0 8.151175774544607 0.0063687291825884895 82-83 2.3808333392854166E-5 2.3808333392854166E-5 0.0 8.867032838834248 0.006761566683570583 84-85 2.3808333392854166E-5 2.3808333392854166E-5 0.0 9.581973282288267 0.006832991683749146 86-87 2.3808333392854166E-5 2.3808333392854166E-5 0.0 10.31455570078639 0.007118691684463396 88 2.3808333392854166E-5 2.3808333392854166E-5 0.0 10.89621709390721 0.007380583351784792 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13690 0.0 25.49178 1 TACATGG 13540 0.0 24.864223 2 ACATGGG 13935 0.0 23.214643 3 GTACTAG 1595 0.0 23.000305 1 CTAGTAC 2085 0.0 21.198364 3 TGATCGC 1080 0.0 20.450361 9 CATGGGG 10345 0.0 20.40708 4 CTGTGCG 2085 0.0 19.608305 9 TACGGGC 1200 0.0 19.199055 4 GTGATCG 1105 0.0 19.138512 8 GTATAAG 2120 0.0 18.857439 1 AGAGTAC 9595 0.0 18.807844 10-11 ATACGGG 1315 0.0 18.235806 3 GCTATTG 2525 0.0 18.053785 8 GTATATG 1930 0.0 18.033255 1 GGCGTCG 1305 0.0 18.006008 8 TAGTACT 2385 0.0 17.742678 4 GTCCTAC 1490 0.0 17.67671 1 ATGGGGG 7175 0.0 17.627058 5 GAGTACA 10545 0.0 17.39473 1 >>END_MODULE