FastQCFastQC Report
Mon 16 Jan 2017
ERR1146512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200210
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT292530.6964651767411629No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176660.42059801771816174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160870.383004659290845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93250.2220127088883651No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT75510.17977672544944182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131050.025.0452061
TACATGG127400.024.654882
GTACTAG17050.023.9939121
ACATGGG132300.023.669813
GAGTACT191550.023.45602612-13
AGAGTAC251200.022.34833510-11
TGATCGC10600.022.1685689
CTAACGC7100.021.8542523
AGTACTT199400.021.837312-13
CATGGGG102150.021.496064
GTGATCG10900.021.1270038
GTACTTT213850.020.81226714-15
TCTAACG8650.019.5696132
CTATTGA26900.019.5676759
GTATAAG21250.019.472871
CGCCTAC8550.019.2384597
ATGGGGG71050.019.0505315
ACTTTTT240350.018.69324316-17
TACGGGC13600.018.6696074
TACTTTT234750.018.51888314-15
Produced by FastQC (version 0.11.5)