##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4200210
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39485406682047	33.0	31.0	34.0	30.0	34.0
2	32.46579528166449	34.0	31.0	34.0	30.0	34.0
3	32.546817659117046	34.0	31.0	34.0	30.0	34.0
4	36.051027924794234	37.0	35.0	37.0	35.0	37.0
5	36.09859983191317	37.0	35.0	37.0	35.0	37.0
6	36.168258253753976	37.0	36.0	37.0	35.0	37.0
7	36.14100652110252	37.0	36.0	37.0	35.0	37.0
8	36.147870939786344	37.0	36.0	37.0	35.0	37.0
9	37.93884567676378	39.0	38.0	39.0	35.0	39.0
10-11	37.88360724820902	39.0	38.0	39.0	35.0	39.0
12-13	37.852562371881405	39.0	38.0	39.0	35.0	39.0
14-15	39.18844391113778	40.0	39.0	41.0	36.0	41.0
16-17	39.15156004104557	40.0	39.0	41.0	36.0	41.0
18-19	39.26851157442128	40.5	39.0	41.0	36.0	41.0
20-21	39.31910416383943	41.0	39.0	41.0	36.0	41.0
22-23	39.27969422957423	41.0	39.0	41.0	36.0	41.0
24-25	39.25335590363339	41.0	39.0	41.0	36.0	41.0
26-27	39.159601543732336	41.0	39.0	41.0	35.5	41.0
28-29	39.10797710114494	40.5	39.0	41.0	35.0	41.0
30-31	38.960731725318496	40.0	38.5	41.0	35.0	41.0
32-33	38.825002916520845	40.0	38.0	41.0	35.0	41.0
34-35	38.73213922637201	40.0	38.0	41.0	35.0	41.0
36-37	38.64255072960638	40.0	38.0	41.0	35.0	41.0
38-39	38.54474276286186	40.0	38.0	41.0	35.0	41.0
40-41	38.452981517590786	40.0	38.0	41.0	34.5	41.0
42-43	38.33688934600889	40.0	38.0	41.0	34.0	41.0
44-45	38.15959571069065	40.0	38.0	41.0	33.5	41.0
46-47	37.881458784203645	40.0	37.5	41.0	33.0	41.0
48-49	37.55432514088581	40.0	37.0	41.0	33.0	41.0
50-51	37.420800031427	40.0	37.0	41.0	32.0	41.0
52-53	37.333612009875694	40.0	36.5	41.0	32.0	41.0
54-55	37.16761554779404	40.0	36.0	41.0	32.0	41.0
56-57	36.989894433849734	39.5	35.5	41.0	31.5	41.0
58-59	36.79530975832161	39.0	35.0	41.0	31.0	41.0
60-61	36.55988771989972	39.0	35.0	41.0	31.0	41.0
62-63	36.33553179483883	38.5	35.0	41.0	31.0	41.0
64-65	36.05719428314299	37.5	35.0	40.0	31.0	41.0
66-67	35.7726939843484	37.0	35.0	40.0	30.5	41.0
68-69	35.453391497091815	36.5	35.0	39.5	30.0	41.0
70-71	35.1264653433995	36.0	35.0	39.0	30.0	41.0
72-73	34.799529309248825	35.5	35.0	39.0	29.5	41.0
74-75	34.504567152594745	35.0	34.0	37.5	29.0	40.0
76-77	34.224718406936795	35.0	34.0	37.0	29.0	39.0
78-79	33.95227524338069	35.0	34.0	37.0	29.0	39.0
80-81	33.706260877432314	35.0	34.0	36.0	29.0	39.0
82-83	33.45902359643922	35.0	34.0	36.0	29.0	37.0
84-85	33.26422476495223	35.0	34.0	35.5	29.0	37.0
86-87	33.11252461186464	35.0	34.0	35.0	29.0	37.0
88-89	32.93151544803712	35.0	34.0	35.0	29.0	36.0
90-91	32.84350282485876	35.0	34.0	35.0	29.0	36.0
92-93	32.743635199192425	35.0	34.0	35.0	28.0	36.0
94-95	32.66761221462736	35.0	34.0	35.0	27.0	36.0
96-97	32.61557612595561	35.0	34.0	35.0	27.0	35.0
98-99	32.55855492939639	35.0	34.0	35.0	27.0	35.0
100	31.813202911283007	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2895641800216211
1101	2	0.28344897621306586
1101	3	0.27643261189733437
1101	4	0.19749000041505838
1101	5	0.17938268826686254
1101	6	0.19611814615002743
1101	7	0.1942962890405795
1101	8	0.23574300733152143
1101	9	0.2924354962125122
1101	10-11	0.27772772272535207
1101	12-13	0.29668752095721374
1101	14-15	0.4461528681013931
1101	16-17	0.46032387652766005
1101	18-19	0.43170565809880657
1101	20-21	0.380304514799775
1101	22-23	0.35389041659666987
1101	24-25	0.3667537939436869
1101	26-27	0.3889587370188963
1101	28-29	0.39815685060811745
1101	30-31	0.45952739960897304
1101	32-33	0.37329463928070084
1101	34-35	0.4193087335280339
1101	36-37	0.41098889902739444
1101	38-39	0.46118545391296095
1101	40-41	0.4090664324815805
1101	42-43	0.43893476570080736
1101	44-45	0.4590870629623467
1101	46-47	0.43903695735720305
1101	48-49	0.5366055238084613
1101	50-51	0.5407198764516039
1101	52-53	0.49222074963611107
1101	54-55	0.5200762167173778
1101	56-57	0.49166006243563487
1101	58-59	0.5625678765380542
1101	60-61	0.5606611498183014
1101	62-63	0.4922812752674517
1101	64-65	0.44949881650754264
1101	66-67	0.4387184109302993
1101	68-69	0.482984292738152
1101	70-71	0.47539110807542784
1101	72-73	0.4590523003346547
1101	74-75	0.4761433238782544
1101	76-77	0.4887678005321874
1101	78-79	0.4727371090808603
1101	80-81	0.42953286365204235
1101	82-83	0.3987306679023135
1101	84-85	0.37781039455160936
1101	86-87	0.4014592454588879
1101	88-89	0.441016011385301
1101	90-91	0.4331222637191843
1101	92-93	0.4529418030839736
1101	94-95	0.3917625872311774
1101	96-97	0.3705158124372474
1101	98-99	0.47922198323103515
1101	100	0.49759289946091556
1102	1	0.2990092577547472
1102	2	0.2717034913736356
1102	3	0.2857124943384406
1102	4	0.1860609653389247
1102	5	0.20376484036286513
1102	6	0.2181283328382193
1102	7	0.20701423196878466
1102	8	0.22667769678394478
1102	9	0.3024941074059768
1102	10-11	0.3126882794736616
1102	12-13	0.2970423766515182
1102	14-15	0.42986956027014855
1102	16-17	0.4648501107830256
1102	18-19	0.42005381024988964
1102	20-21	0.39866091881794574
1102	22-23	0.3831603371882508
1102	24-25	0.3130531859110164
1102	26-27	0.33635493850921705
1102	28-29	0.3726110176876887
1102	30-31	0.4673913959835403
1102	32-33	0.4462711274341302
1102	34-35	0.42972326486270873
1102	36-37	0.437724560395381
1102	38-39	0.3886562566494547
1102	40-41	0.396730917856182
1102	42-43	0.3777298756729337
1102	44-45	0.38425714955956636
1102	46-47	0.4687436788861419
1102	48-49	0.4642392727636775
1102	50-51	0.436671685300567
1102	52-53	0.37792533416772045
1102	54-55	0.4063550875218809
1102	56-57	0.3871331105370288
1102	58-59	0.3663493138613987
1102	60-61	0.3325209576286383
1102	62-63	0.2895816731027594
1102	64-65	0.2909970069821384
1102	66-67	0.33857567546795053
1102	68-69	0.3397073531150241
1102	70-71	0.31487086686215093
1102	72-73	0.26953122040588795
1102	74-75	0.23492083888644544
1102	76-77	0.2646808332973052
1102	78-79	0.289624566071204
1102	80-81	0.3133931394069691
1102	82-83	0.2276760764037249
1102	84-85	0.22840964038753953
1102	86-87	0.24551408287904763
1102	88-89	0.26691279244979427
1102	90-91	0.26849648513636026
1102	92-93	0.2864142184231895
1102	94-95	0.2843115310148505
1102	96-97	0.3233846444910853
1102	98-99	0.3176740185324931
1102	100	0.29925337482367453
1103	1	0.21701249500981845
1103	2	0.2083495026574269
1103	3	0.23765766220956408
1103	4	0.1485985347769514
1103	5	0.1437259904069066
1103	6	0.14438670584581814
1103	7	0.15189590021656585
1103	8	0.14074382717659262
1103	9	0.21821395232964846
1103	10-11	0.21551392162780303
1103	12-13	0.22781666604785045
1103	14-15	0.346100180566971
1103	16-17	0.40266565181645575
1103	18-19	0.31896217047984265
1103	20-21	0.31403708427650656
1103	22-23	0.33466666589069405
1103	24-25	0.3438222767679875
1103	26-27	0.33654281913426587
1103	28-29	0.35037340019464125
1103	30-31	0.4132745225600729
1103	32-33	0.3975373698408262
1103	34-35	0.4392776421036544
1103	36-37	0.39959917445811044
1103	38-39	0.38905980428124565
1103	40-41	0.4285524454545282
1103	42-43	0.437911134768612
1103	44-45	0.4814387136131373
1103	46-47	0.5903273660710227
1103	48-49	0.5964236533745435
1103	50-51	0.6051496590232048
1103	52-53	0.5394173300691847
1103	54-55	0.5810097567652903
1103	56-57	0.5541821625006165
1103	58-59	0.5211105544204386
1103	60-61	0.4909458153068229
1103	62-63	0.5048255904891548
1103	64-65	0.43349126805587446
1103	66-67	0.4484978304878311
1103	68-69	0.4761845251549275
1103	70-71	0.42716178732050025
1103	72-73	0.46416771238369137
1103	74-75	0.39605337404258734
1103	76-77	0.46607665370312645
1103	78-79	0.42646993126969335
1103	80-81	0.4038001601791308
1103	82-83	0.4101198537442414
1103	84-85	0.339297364304322
1103	86-87	0.4193541517036792
1103	88-89	0.46182327766138087
1103	90-91	0.4004286642114394
1103	92-93	0.41750799141953365
1103	94-95	0.37049769656245957
1103	96-97	0.39041277642459704
1103	98-99	0.38960934190571805
1103	100	0.40378920426148923
1104	1	0.23815628527470523
1104	2	0.1930152942465071
1104	3	0.19955661215155374
1104	4	0.12139867428410867
1104	5	0.11276047176203008
1104	6	0.1393322029681272
1104	7	0.14600947714708212
1104	8	0.1525743042272083
1104	9	0.12720991834126494
1104	10-11	0.1979781597611776
1104	12-13	0.1731680444353998
1104	14-15	0.3089049533538173
1104	16-17	0.3346106064889014
1104	18-19	0.28726512521755154
1104	20-21	0.2720892366550913
1104	22-23	0.31074466218056074
1104	24-25	0.2699468163785781
1104	26-27	0.25641273818432353
1104	28-29	0.27288425357774315
1104	30-31	0.29754494153417
1104	32-33	0.32896105861916425
1104	34-35	0.2612671594180114
1104	36-37	0.26775026253323375
1104	38-39	0.24408977235530216
1104	40-41	0.22285346758304314
1104	42-43	0.29800152670441804
1104	44-45	0.2564145209108091
1104	46-47	0.2972156722766073
1104	48-49	0.3786205174108588
1104	50-51	0.3222204350458
1104	52-53	0.27152148465265213
1104	54-55	0.31633711964143885
1104	56-57	0.2185069264594901
1104	58-59	0.21047559861466425
1104	60-61	0.22716979060815845
1104	62-63	0.23253966432620388
1104	64-65	0.20601271780750352
1104	66-67	0.1768831246230249
1104	68-69	0.16039375185511062
1104	70-71	0.21197489299331096
1104	72-73	0.20655394089402535
1104	74-75	0.16072750237724165
1104	76-77	0.19148044727323565
1104	78-79	0.15012799943942667
1104	80-81	0.15940509527988667
1104	82-83	0.11130476107690157
1104	84-85	0.20093703654671202
1104	86-87	0.1637218046396285
1104	88-89	0.21183761960057979
1104	90-91	0.1570624470483466
1104	92-93	0.1864126409446385
1104	94-95	0.2131396501819509
1104	96-97	0.1602275908495585
1104	98-99	0.15585011876689947
1104	100	0.26282004706225237
1105	1	0.21363442336149774
1105	2	0.1762434022436281
1105	3	0.19311465777456505
1105	4	0.1513234014416298
1105	5	0.1013036097249298
1105	6	0.1256297888918283
1105	7	0.14673450537826938
1105	8	0.1366527771942998
1105	9	0.2063069141534939
1105	10-11	0.20417198500747702
1105	12-13	0.1994562914998923
1105	14-15	0.3322821357396819
1105	16-17	0.38630061698489015
1105	18-19	0.3263610612539978
1105	20-21	0.2790129575787361
1105	22-23	0.3245445358039589
1105	24-25	0.2819280904281882
1105	26-27	0.2874258373669534
1105	28-29	0.28642277852915754
1105	30-31	0.271666389625949
1105	32-33	0.26957251826892303
1105	34-35	0.28740529001778725
1105	36-37	0.29315888463887774
1105	38-39	0.3054389532083661
1105	40-41	0.2701149870581432
1105	42-43	0.31274275335395885
1105	44-45	0.3188836015652754
1105	46-47	0.33847109505412476
1105	48-49	0.4020152889771822
1105	50-51	0.4373778504672856
1105	52-53	0.4164036194437415
1105	54-55	0.385617693398288
1105	56-57	0.3445679190999087
1105	58-59	0.28157493788096843
1105	60-61	0.33216508467270245
1105	62-63	0.2940953797699848
1105	64-65	0.29714917577577893
1105	66-67	0.3689738454121496
1105	68-69	0.29185893722515743
1105	70-71	0.3286535512773554
1105	72-73	0.31470732253012557
1105	74-75	0.3711040568625492
1105	76-77	0.2775656576708059
1105	78-79	0.28445341786279954
1105	80-81	0.2904585389537999
1105	82-83	0.2515531977416927
1105	84-85	0.271360409226709
1105	86-87	0.3145684969002147
1105	88-89	0.3211377826157715
1105	90-91	0.2923804373063632
1105	92-93	0.3234368370195284
1105	94-95	0.3442101299174496
1105	96-97	0.27249573447638653
1105	98-99	0.3535044411475923
1105	100	0.3905617467390563
1106	1	0.09679998026879844
1106	2	0.09512252718117509
1106	3	0.11570896921685403
1106	4	0.1404545576189875
1106	5	0.11756726899802317
1106	6	0.11495108866806447
1106	7	0.13968126951547788
1106	8	0.12083829890792686
1106	9	0.10467630108838222
1106	10-11	0.15786161808084387
1106	12-13	0.1559586621848652
1106	14-15	0.2419594591738914
1106	16-17	0.28985516446376636
1106	18-19	0.22004861692300892
1106	20-21	0.19445830962409616
1106	22-23	0.20333301530690306
1106	24-25	0.21945525961852042
1106	26-27	0.20817510144985363
1106	28-29	0.2272101690920394
1106	30-31	0.19579966394108794
1106	32-33	0.14693072523814266
1106	34-35	0.17262284720750642
1106	36-37	0.15941545629710419
1106	38-39	0.12396090037439933
1106	40-41	0.15848476774940679
1106	42-43	0.21019749852568737
1106	44-45	0.18721425101890787
1106	46-47	0.19464406815920654
1106	48-49	0.20593255397251653
1106	50-51	0.12536362364188136
1106	52-53	0.12041905197833813
1106	54-55	0.1520440147903983
1106	56-57	0.09811387182742237
1106	58-59	0.12958597935301697
1106	60-61	0.15171268574589902
1106	62-63	0.15815606713285035
1106	64-65	0.153958214792965
1106	66-67	0.18663532626201373
1106	68-69	0.06653369445398027
1106	70-71	0.11106817403157976
1106	72-73	0.10405766523395954
1106	74-75	0.14552091723924576
1106	76-77	0.16008037601280023
1106	78-79	0.18119046735119326
1106	80-81	0.12077172463301622
1106	82-83	0.09778509852593942
1106	84-85	0.03911394760302045
1106	86-87	0.09350652236131651
1106	88-89	0.18013734269485582
1106	90-91	0.08349476786620613
1106	92-93	0.08164561208415932
1106	94-95	0.1241513380163326
1106	96-97	0.10466482975999725
1106	98-99	0.10523820480685941
1106	100	0.1235242068203064
1107	1	0.09898346211954134
1107	2	0.10822906816645883
1107	3	0.1028300527326067
1107	4	0.053356362690216486
1107	5	0.06148016623063768
1107	6	0.09024108522557839
1107	7	0.07673008711367402
1107	8	0.0953847974271298
1107	9	0.11741969926833207
1107	10-11	0.1506999999549663
1107	12-13	0.1066650155415445
1107	14-15	0.17813448985405245
1107	16-17	0.24939435755566564
1107	18-19	0.14678269674137567
1107	20-21	0.17149204335441226
1107	22-23	0.19456735659650093
1107	24-25	0.18216022546958754
1107	26-27	0.22126942292544527
1107	28-29	0.19089858210621458
1107	30-31	0.2098862227432079
1107	32-33	0.1429392421050082
1107	34-35	0.09001315411428834
1107	36-37	0.08401943906891063
1107	38-39	0.10220883291219707
1107	40-41	0.1253370877481501
1107	42-43	0.14297844870424825
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2216	56-57	-0.4464582567043678
2216	58-59	-0.5252137504828269
2216	60-61	-0.5851704053156084
2216	62-63	-0.6102005275599822
2216	64-65	-0.5030578002259247
2216	66-67	-0.563426449807487
2216	68-69	-0.49763047023024853
2216	70-71	-0.45191296343541154
2216	72-73	-0.4066965063742245
2216	74-75	-0.4297680654788323
2216	76-77	-0.4382590536650923
2216	78-79	-0.3973906813060353
2216	80-81	-0.4273696679312735
2216	82-83	-0.46668270108499854
2216	84-85	-0.42080487326635563
2216	86-87	-0.40787064537125417
2216	88-89	-0.3381970214052856
2216	90-91	-0.44096240102535234
2216	92-93	-0.36183646805987024
2216	94-95	-0.3419000890878081
2216	96-97	-0.3981521909808947
2216	98-99	-0.4138744398543466
2216	100	-0.5161153478484692
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	32.0
8	125.0
9	461.0
10	554.0
11	361.0
12	320.0
13	402.0
14	655.0
15	1292.0
16	2328.0
17	4196.0
18	7062.0
19	10497.0
20	15017.0
21	22006.0
22	31128.0
23	35022.0
24	32977.0
25	28000.0
26	25845.0
27	28398.0
28	34701.0
29	42013.0
30	51613.0
31	64637.0
32	82470.0
33	107444.0
34	155791.0
35	245853.0
36	443811.0
37	1009927.0
38	1277686.0
39	436163.0
40	1421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32884374593014	19.67005937255139	13.470976448356215	23.53012043316225
2	15.213904800090287	21.487836085604776	39.10935737370495	24.188901740599984
3	18.846275085686315	26.350148707062797	31.066514864516105	23.73706134273478
4	12.176725267333186	17.75184772656029	39.34857217448141	30.722854831625114
5	12.31178451210517	38.726713087717314	35.27870487078184	13.682797529395687
6	29.003149043120413	38.810849317834304	17.701617569117758	14.48438406992752
7	24.91211746844026	33.93631126451089	23.329434321006406	17.82213694604245
8	25.24973221506886	33.799779486638606	20.76611131996365	20.18437697832888
9	25.67109803662011	15.366579639189126	21.706459159384085	37.25586316480668
10-11	24.522544308238178	25.819516254736524	28.520288228685896	21.1376512083394
12-13	26.191261865478154	24.211015639694207	28.13384330783461	21.463879186993033
14-15	22.81861721864522	25.181999893576712	27.55751291870392	24.441869969074155
16-17	21.179542292897214	27.983199359458617	28.035839747516604	22.801418600127565
18-19	20.891978316286142	27.310049061914388	30.40356620858563	21.394406413213837
20-21	22.39836074394588	25.761047455381863	29.92805339176867	21.912538408903583
22-23	21.99716204665957	25.759331081064996	29.96206142073849	22.281445451536946
24-25	22.377500172610418	25.900085948083547	30.071317862678292	21.65109601662774
26-27	22.06542003451505	25.612194572325365	30.56922455889861	21.753160834260967
28-29	21.308044277315215	27.03213970636465	29.987361344713875	21.67245467160626
30-31	21.965405239390243	25.890882641967856	29.905641454191787	22.238070664450106
32-33	22.101866827992335	26.155364763030896	29.77584755200319	21.96692085697358
34-35	22.644533770804202	25.9617539959671	29.86339828534498	21.530313947883716
36-37	22.31478243106474	25.609178019474744	30.345299483906636	21.73074006555388
38-39	22.093361143295628	26.119135316672036	30.170729851993016	21.616773688039316
40-41	22.01604477136659	26.11024587134208	29.86952120793741	22.004188149353915
42-43	21.990186204975466	26.14896636120575	29.05247594763119	22.808371486187596
44-45	22.157275378453388	26.070434483036113	29.126786497839262	22.645503640671237
46-47	22.840679394601697	26.30555376993055	28.72115918013623	22.13260765533152
48-49	22.953781666158953	26.28220214463316	28.451917412136147	22.31209877707174
50-51	21.88042978803441	26.729484954323713	28.774073201101853	22.61601205654003
52-53	22.2229549919974	27.21975573767662	27.731981650862846	22.825307619463132
54-55	21.9645089822733	26.605216264647108	28.004224571296128	23.426050181783463
56-57	22.78565140609292	26.16194100165409	27.818218288284914	23.234189303968083
58-59	22.96351681903308	26.22941101123713	28.08712812920964	22.719944040520147
60-61	22.30264463445466	26.638217115990287	28.626368347954323	22.43276990160073
62-63	22.07425976179241	26.514331929750046	28.256986211615537	23.15442209684201
64-65	21.58258222688603	27.196348738778042	27.920363173830726	23.300705860505204
66-67	22.2329427324365	26.221678855708213	27.71104144432851	23.834336967526774
68-69	21.746685132924437	26.190372451276673	28.144852983017284	23.91808943278161
70-71	22.428759470572906	26.12576351778711	27.723256594952034	23.722220416687946
72-73	22.409117477943795	25.664317099854404	28.66969915941777	23.25686626278403
74-75	21.824780814116277	27.07214705977589	27.78677494376308	23.31629718234475
76-77	21.971545809133016	26.094413018623268	27.646574511418294	24.287466660825416
78-79	22.068963218473996	26.147506090255778	27.810564950982723	23.972965740287496
80-81	22.239061521801005	26.134852195564385	27.855309849395077	23.770776433239533
82-83	22.17039103618088	25.60034417621746	28.66194352900618	23.567321258595477
84-85	22.09159419158088	26.155837644062313	27.886859107671363	23.865709056685443
86-87	21.510269724609007	26.499686920415883	27.81194273619652	24.178100618778583
88-89	21.646489104116224	26.101194940252988	27.342942376690687	24.9093735789401
90-91	21.928478763070487	25.50656431932998	27.606591770357987	24.95836514724155
92-93	22.313209801326458	25.94901870312582	27.594901870312583	24.142869625235136
94-95	21.473379588984592	25.691159305820012	28.00046188194274	24.83499922325266
96-97	20.983240649089296	25.741951112954943	28.310833372518047	24.963974865437716
98-99	21.26368072617382	26.15601161514321	27.392760332140814	25.18754732654216
100	21.51347242789627	24.97051994267204	27.432167275647174	26.083840353784517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	556.0
1	362.0
2	564.5
3	902.0
4	566.5
5	432.0
6	992.0
7	2600.0
8	4737.5
9	5588.5
10	4102.5
11	2487.0
12	2398.0
13	2726.0
14	3113.5
15	3597.5
16	4133.5
17	4852.5
18	5715.0
19	6470.5
20	7450.0
21	8806.5
22	10604.5
23	12454.5
24	14588.0
25	17575.0
26	22472.0
27	28715.0
28	35248.0
29	42413.5
30	49187.5
31	56834.0
32	66221.5
33	73032.0
34	78827.5
35	86231.0
36	94581.5
37	104784.5
38	111339.0
39	112872.0
40	113091.5
41	112836.0
42	114548.5
43	120210.0
44	127789.0
45	139871.5
46	153068.0
47	180634.0
48	224660.5
49	217155.0
50	175848.5
51	161354.0
52	156255.0
53	152010.0
54	146722.0
55	134053.0
56	121747.0
57	106795.0
58	90244.0
59	80168.0
60	67000.0
61	52730.5
62	42342.0
63	32800.5
64	23245.0
65	15599.5
66	11115.0
67	8466.0
68	6304.5
69	4592.0
70	3631.5
71	2965.0
72	2219.5
73	1868.0
74	1519.0
75	1158.5
76	861.5
77	589.5
78	434.0
79	278.5
80	182.5
81	135.5
82	91.0
83	59.5
84	42.0
85	25.5
86	17.0
87	10.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002880808340535354
2	0.0039045666764280836
3	1.42850000357125E-4
4	0.04947371679035095
5	0.0024760666728568335
6	0.00485690001214225
7	2.3808333392854166E-5
8	2.6189166732139583E-4
9	9.523333357141666E-5
10-11	8.332916687498958E-5
12-13	0.0
14-15	3.571250008928125E-5
16-17	3.095083341071042E-4
18-19	1.6665833374997915E-4
20-21	2.8570000071425E-4
22-23	0.0
24-25	0.0
26-27	3.214125008035312E-4
28-29	1.1904166696427083E-5
30-31	1.7856250044640624E-4
32-33	2.4998750062496874E-4
34-35	1.547541670535521E-4
36-37	2.3808333392854166E-5
38-39	8.690041688391771E-4
40-41	6.071125015177812E-4
42-43	0.0
44-45	5.475916680356458E-4
46-47	0.0
48-49	1.0713750026784375E-4
50-51	0.0
52-53	2.976041674106771E-4
54-55	4.8807083455351045E-4
56-57	1.7856250044640624E-4
58-59	6.666333349999166E-4
60-61	6.309208349106354E-4
62-63	1.9046666714283333E-4
64-65	4.166458343749479E-4
66-67	0.0013689791700891144
68-69	0.0027855750069639374
70-71	0.0038212375095530936
72-73	0.003452208341963854
74-75	0.0029046166739282084
76-77	0.004666433344999416
78-79	0.0013808833367855418
80-81	0.0011547041695534272
82-83	8.5710000214275E-4
84-85	7.380583351784791E-4
86-87	0.0
88-89	0.0
90-91	7.14250001785625E-5
92-93	7.14250001785625E-5
94-95	5.952083348213542E-5
96-97	4.166458343749479E-4
98-99	8.332916687498958E-5
100	0.07868654186338302
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4200210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.19212508000983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.48222957575149	32.03919923312545
2	15.776167601409124	15.205740846606144
3	6.900874945758148	9.977034856424483
4	3.669498753737119	7.073637716841577
5	2.139217832725224	5.154672668404077
6	1.3384465264478513	3.870154944928773
7	0.8777879586648458	2.961172696839192
8	0.6393570218929004	2.4649578855876193
9	0.4349368238237286	1.8864476834053794
>10	1.696400449295981	12.64046959748201
>50	0.02978719913043648	0.9623040719651322
>100	0.012478383943164088	1.1835893716541523
>500	0.0013657829914841858	0.46760985421313006
>1k	0.00102433724361107	0.8268635585176379
>5k	1.7072287393517834E-4	0.6942362892310278
>10k+	2.560843109027675E-4	2.5919087247742434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	29253	0.6964651767411629	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17666	0.42059801771816174	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16087	0.383004659290845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9325	0.2220127088883651	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	7551	0.17977672544944182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.142750005356875E-4
2	0.0	0.0	0.0	0.0	2.142750005356875E-4
3	0.0	0.0	0.0	0.0	2.142750005356875E-4
4	0.0	0.0	0.0	9.523333357141666E-5	2.142750005356875E-4
5	0.0	0.0	0.0	9.523333357141666E-5	2.3808333392854166E-4
6	0.0	0.0	0.0	1.6665833374997918E-4	3.095083341071042E-4
7	0.0	0.0	0.0	1.6665833374997918E-4	3.095083341071042E-4
8	0.0	0.0	0.0	1.6665833374997918E-4	3.095083341071042E-4
9	0.0	0.0	0.0	2.142750005356875E-4	3.095083341071042E-4
10-11	0.0	0.0	0.0	2.2617916723211457E-4	3.3331666749995836E-4
12-13	0.0	0.0	0.0	2.3808333392854166E-4	0.0011666083362498543
14-15	0.0	0.0	0.0	2.6189166732139583E-4	0.0014404041702676773
16-17	0.0	0.0	0.0	7.380583351784791E-4	0.001690391670892646
18-19	0.0	0.0	0.0	9.523333357141666E-4	0.001726104170981927
20-21	0.0	0.0	0.0	9.99950002499875E-4	0.0019284750048211875
22-23	0.0	1.1904166696427083E-5	0.0	0.0012261291697319896	0.0020951333385711667
24-25	0.0	2.3808333392854166E-5	0.0	0.0017618166710712084	0.002118941671964021
26-27	0.0	2.3808333392854166E-5	0.0	0.0029641375074103437	0.002273695839017573
28-29	0.0	2.3808333392854166E-5	0.0	0.008451958354463229	0.002297504172410427
30-31	0.0	2.3808333392854166E-5	0.0	0.023451208391961353	0.0023451208391961353
32-33	0.0	2.3808333392854166E-5	0.0	0.05685430014213575	0.002452258339463979
34-35	0.0	2.3808333392854166E-5	0.0	0.11050637944293262	0.002761766673571083
36-37	0.0	2.3808333392854166E-5	0.0	0.1914666171453332	0.002761766673571083
38-39	0.0	2.3808333392854166E-5	0.0	0.31016306327540766	0.0028450958404460727
40-41	0.0	2.3808333392854166E-5	0.0	0.43890662609726655	0.002880808340535354
42-43	0.0	2.3808333392854166E-5	0.0	0.5736141764340354	0.0029165208406246356
44-45	0.0	2.3808333392854166E-5	0.0	0.7434747310253536	0.0030712750076781876
46-47	0.0	2.3808333392854166E-5	0.0	0.9504881898762204	0.003273645841517448
48-49	0.0	2.3808333392854166E-5	0.0	1.165905990414765	0.003523633342142417
50-51	0.0	2.3808333392854166E-5	0.0	1.39621590349054	0.003690291675892396
52-53	0.0	2.3808333392854166E-5	0.0	1.6290137874058679	0.0039998000099995
54-55	0.0	2.3808333392854166E-5	0.0	1.8841557922103895	0.004106937510267344
56-57	0.0	2.3808333392854166E-5	0.0	2.205663526347492	0.004214075010535187
58-59	0.0	2.3808333392854166E-5	0.0	2.548015456370039	0.004309308344106604
60-61	0.0	2.3808333392854166E-5	0.0	2.906414203099369	0.004333116677499458
62-63	0.0	2.3808333392854166E-5	0.0	3.2774551748603047	0.004642625011606562
64-65	0.0	2.3808333392854166E-5	0.0	3.661602634154007	0.004714050011785125
66-67	0.0	2.3808333392854166E-5	0.0	4.105842326931272	0.004785475011963688
68-69	0.0	2.3808333392854166E-5	0.0	4.604579294844782	0.0048330916787493955
70-71	0.0	2.3808333392854166E-5	0.0	5.143968992026589	0.004892612512231531
72-73	0.0	2.3808333392854166E-5	0.0	5.67704948085929	0.0051068875127672185
74-75	0.0	2.3808333392854166E-5	0.0	6.223653103059133	0.0053449708466957605
76-77	0.0	2.3808333392854166E-5	0.0	6.809754750357721	0.00551162918044574
78-79	0.0	2.3808333392854166E-5	0.0	7.48534001871335	0.0056544791808028644
80-81	0.0	2.3808333392854166E-5	0.0	8.195292616321565	0.005737808347677854
82-83	0.0	2.3808333392854166E-5	0.0	8.912601988948172	0.006047316681784958
84-85	0.0	2.3808333392854166E-5	0.0	9.628566190738082	0.0060949333485706665
86-87	0.0	2.3808333392854166E-5	0.0	10.363481825908703	0.0063330166824992085
88	0.0	2.3808333392854166E-5	0.0	10.946738377366845	0.006547291683034896
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	13105	0.0	25.045206	1
TACATGG	12740	0.0	24.65488	2
GTACTAG	1705	0.0	23.993912	1
ACATGGG	13230	0.0	23.66981	3
GAGTACT	19155	0.0	23.456026	12-13
AGAGTAC	25120	0.0	22.348335	10-11
TGATCGC	1060	0.0	22.168568	9
CTAACGC	710	0.0	21.854252	3
AGTACTT	19940	0.0	21.8373	12-13
CATGGGG	10215	0.0	21.49606	4
GTGATCG	1090	0.0	21.127003	8
GTACTTT	21385	0.0	20.812267	14-15
TCTAACG	865	0.0	19.569613	2
CTATTGA	2690	0.0	19.567675	9
GTATAAG	2125	0.0	19.47287	1
CGCCTAC	855	0.0	19.238459	7
ATGGGGG	7105	0.0	19.050531	5
ACTTTTT	24035	0.0	18.693243	16-17
TACGGGC	1360	0.0	18.669607	4
TACTTTT	23475	0.0	18.518883	14-15
>>END_MODULE