FastQCFastQC Report
Mon 16 Jan 2017
ERR1146511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881155
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.040.8848571
GTATCAA45500.037.3113371
CAACGCA47300.033.590145
ATCAACG48300.032.8007973
TCAACGC48800.032.5604864
AACGCAG51200.031.3009626
TATCAAC56350.029.0326672
ACGCAGA59800.026.5637157
CGCAGAG61650.025.3091938
GTACATG84200.024.4628471
TACATGG81550.023.7506142
ACATGGG80450.022.9650863
GCAGAGT72000.021.082439
CATGGGG56250.020.392074
TATACTG14900.017.0358495
GAGTACA63700.016.9798341
AGAGTAC60950.016.88563210-11
CAGAGTA68700.016.65670610-11
GTATTAT14450.016.2722321
ATGGGGG36250.016.0794055
Produced by FastQC (version 0.11.5)