##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146511_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2881155
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.014978020967284	31.0	31.0	34.0	28.0	34.0
2	31.047033568135003	31.0	31.0	34.0	27.0	34.0
3	31.276685565337512	31.0	31.0	34.0	28.0	34.0
4	34.78399253077325	37.0	35.0	37.0	32.0	37.0
5	34.80666815912368	37.0	35.0	37.0	32.0	37.0
6	34.79105844704641	37.0	35.0	37.0	32.0	37.0
7	34.80279853045046	37.0	35.0	37.0	32.0	37.0
8	34.74656483250641	37.0	35.0	37.0	31.0	37.0
9	36.3448082453044	39.0	37.0	39.0	32.0	39.0
10-11	36.34696380444648	39.0	37.0	39.0	32.0	39.0
12-13	36.28896831305501	39.0	37.0	39.0	31.0	39.0
14-15	37.60834769389359	40.0	38.0	41.0	31.5	41.0
16-17	37.62284153403756	40.0	38.0	41.0	31.0	41.0
18-19	37.62653866244614	40.0	38.0	41.0	31.0	41.0
20-21	37.745105869000454	40.0	38.0	41.0	31.0	41.0
22-23	37.7543842660322	40.0	38.0	41.0	31.0	41.0
24-25	37.77683411687327	40.0	38.0	41.0	31.0	41.0
26-27	37.852647115479726	40.0	38.0	41.0	31.0	41.0
28-29	37.83719359076481	40.0	38.0	41.0	31.0	41.0
30-31	37.80150460492407	40.0	38.0	41.0	31.0	41.0
32-33	37.77791354508869	40.0	38.0	41.0	31.0	41.0
34-35	37.72395619118027	40.0	38.0	41.0	31.0	41.0
36-37	37.64142123558087	40.0	38.0	41.0	30.0	41.0
38-39	37.58675687354551	40.0	38.0	41.0	30.0	41.0
40-41	37.54156753107695	40.0	37.0	41.0	30.0	41.0
42-43	37.38121586655352	40.0	37.0	41.0	30.0	41.0
44-45	37.253769061366015	40.0	37.0	41.0	30.0	41.0
46-47	37.146477367583486	40.0	36.5	41.0	30.0	41.0
48-49	37.04599908717164	40.0	36.0	41.0	30.0	41.0
50-51	36.93368631677227	40.0	36.0	41.0	30.0	41.0
52-53	36.81812432861127	39.5	35.5	41.0	30.0	41.0
54-55	36.710490584505166	39.0	35.0	41.0	29.5	41.0
56-57	36.54906469801173	39.0	35.0	41.0	29.0	41.0
58-59	36.38196730130798	39.0	35.0	41.0	29.0	41.0
60-61	36.17462389215436	39.0	35.0	40.5	29.0	41.0
62-63	35.954157447273744	38.0	35.0	40.0	28.5	41.0
64-65	35.67768967653598	37.5	34.5	40.0	28.0	41.0
66-67	35.44667381657703	37.0	34.0	40.0	28.0	41.0
68-69	35.19834562874958	37.0	34.0	39.5	28.0	41.0
70-71	34.900652342550124	36.0	34.0	39.0	28.0	41.0
72-73	34.55916116973922	36.0	34.0	39.0	27.5	40.5
74-75	34.21996855427771	35.0	34.0	38.5	27.0	40.0
76-77	33.94298692711777	35.0	33.5	37.0	27.0	39.5
78-79	33.62381475484658	35.0	33.0	37.0	27.0	39.0
80-81	33.36517282131645	35.0	33.0	36.5	26.5	39.0
82-83	33.09252574054503	35.0	33.0	36.0	26.0	37.5
84-85	32.876295096931614	35.0	33.0	36.0	26.0	37.0
86-87	32.66461124097801	35.0	33.0	35.0	26.0	37.0
88-89	32.475675553727584	35.0	33.0	35.0	26.0	36.0
90-91	32.3335224935833	35.0	33.0	35.0	25.0	36.0
92-93	32.185122459569165	35.0	33.0	35.0	25.0	36.0
94-95	32.05425879551777	35.0	32.0	35.0	25.0	36.0
96-97	31.965957228958526	35.0	32.0	35.0	25.0	35.0
98-99	31.86532709972216	35.0	32.0	35.0	25.0	35.0
100	30.70135449151469	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27515122479152865
1101	2	0.20709373112589802
1101	3	0.22585709541968058
1101	4	0.1835610169754176
1101	5	0.20987977054418394
1101	6	0.18969459403316336
1101	7	0.27371642203608104
1101	8	0.3265367729051434
1101	9	0.3866968212644224
1101	10-11	0.4080354101882193
1101	12-13	0.3782976841488477
1101	14-15	0.572580452142077
1101	16-17	0.47127926978419055
1101	18-19	0.4703286955693855
1101	20-21	0.5030565322857541
1101	22-23	0.5247996846844956
1101	24-25	0.5645599264933736
1101	26-27	0.5450036739740654
1101	28-29	0.5249065067389438
1101	30-31	0.5747380857324345
1101	32-33	0.4868179850337597
1101	34-35	0.4395835819633547
1101	36-37	0.5216569649200977
1101	38-39	0.464049626777296
1101	40-41	0.41732185514825915
1101	42-43	0.4654413083742881
1101	44-45	0.3971727058579475
1101	46-47	0.36013794220585993
1101	48-49	0.40729350607897175
1101	50-51	0.46064579115129334
1101	52-53	0.3784374872701406
1101	54-55	0.37200055193181214
1101	56-57	0.4663935167089903
1101	58-59	0.3744383761444112
1101	60-61	0.3314226844773671
1101	62-63	0.39967979307006374
1101	64-65	0.46655701753861223
1101	66-67	0.32535879227125264
1101	68-69	0.3387287180933569
1101	70-71	0.28087194924228953
1101	72-73	0.24143221228607814
1101	74-75	0.3291986000345517
1101	76-77	0.270357538243033
1101	78-79	0.2935238236927944
1101	80-81	0.2683404948629331
1101	82-83	0.27503406781829653
1101	84-85	0.23803981551012754
1101	86-87	0.19150159431983127
1101	88-89	0.1931516090972636
1101	90-91	0.23304432327286406
1101	92-93	0.2818552456192833
1101	94-95	0.33396001941861897
1101	96-97	0.3218113829632472
1101	98-99	0.24682617255247763
1101	100	0.20978011379810368
1102	1	0.21275638794450202
1102	2	0.20961757896103705
1102	3	0.2198995799089225
1102	4	0.28074981476775207
1102	5	0.2819163777003695
1102	6	0.2634475262552485
1102	7	0.4073817912213258
1102	8	0.3910767277804723
1102	9	0.39064869008539205
1102	10-11	0.3614202018427335
1102	12-13	0.37838397186411044
1102	14-15	0.4564766002638905
1102	16-17	0.33904738240322274
1102	18-19	0.37742440167017577
1102	20-21	0.3752436680898654
1102	22-23	0.35352213129397114
1102	24-25	0.4487579798642187
1102	26-27	0.4303834348919793
1102	28-29	0.4375946675488578
1102	30-31	0.43391093793487556
1102	32-33	0.414500773212346
1102	34-35	0.4104722640270424
1102	36-37	0.4572915188085389
1102	38-39	0.4560108271533849
1102	40-41	0.38925748804015825
1102	42-43	0.42634184821519483
1102	44-45	0.3175593996410342
1102	46-47	0.2936685546722657
1102	48-49	0.29915929476361214
1102	50-51	0.2911525347533157
1102	52-53	0.25682170231649337
1102	54-55	0.1594873069031948
1102	56-57	0.2731649655150079
1102	58-59	0.2741691001336193
1102	60-61	0.2476891059385835
1102	62-63	0.17314408203079523
1102	64-65	0.22834950236703122
1102	66-67	0.21708405815545007
1102	68-69	0.21400677889905495
1102	70-71	0.10985281806189562
1102	72-73	0.11152225812982408
1102	74-75	0.15781915659031398
1102	76-77	0.13725378540578248
1102	78-79	0.17131453935171237
1102	80-81	0.14157146102756712
1102	82-83	0.11571333258184069
1102	84-85	0.05912668119027842
1102	86-87	0.060725405116386355
1102	88-89	0.14381464064518923
1102	90-91	0.06800170175439035
1102	92-93	0.11933182515254259
1102	94-95	0.13863797358573748
1102	96-97	0.16559802302001003
1102	98-99	0.23807187003805907
1102	100	0.27743063227059395
1103	1	0.12401445721918947
1103	2	0.1407219604090706
1103	3	0.13955377424329285
1103	4	0.14884816781049182
1103	5	0.16456538051591707
1103	6	0.13526959230792812
1103	7	0.18408281001750026
1103	8	0.1966800390823238
1103	9	0.3779498111304562
1103	10-11	0.24724490718761416
1103	12-13	0.22399833166561933
1103	14-15	0.2578864568737771
1103	16-17	0.29021158325365093
1103	18-19	0.2427028868244605
1103	20-21	0.16340892307182742
1103	22-23	0.18183438210419922
1103	24-25	0.2229697995290465
1103	26-27	0.22666712020075153
1103	28-29	0.21391922921297635
1103	30-31	0.20390072142384952
1103	32-33	0.3010273843783082
1103	34-35	0.2683992499681125
1103	36-37	0.24486245937264783
1103	38-39	0.2203300055739561
1103	40-41	0.1893145414509334
1103	42-43	0.22344672756749873
1103	44-45	0.1327865186809376
1103	46-47	0.14959216226260708
1103	48-49	0.16675967908951606
1103	50-51	0.19447606832179787
1103	52-53	0.1606362122884022
1103	54-55	0.16732630247445002
1103	56-57	0.1301525969825832
1103	58-59	0.14394802509475113
1103	60-61	0.22877757262349974
1103	62-63	0.24842603498586868
1103	64-65	0.1715812619830288
1103	66-67	0.18302417727225162
1103	68-69	0.22487966515009816
1103	70-71	0.12720735329249067
1103	72-73	0.1490593165853653
1103	74-75	0.17311095936273801
1103	76-77	0.12298940158873961
1103	78-79	0.14204835654453518
1103	80-81	0.1304870331286665
1103	82-83	0.17113044534414001
1103	84-85	0.09695306675261861
1103	86-87	0.17460805373252697
1103	88-89	0.06730803394013662
1103	90-91	0.1383058364543146
1103	92-93	0.0472902133476083
1103	94-95	0.049059575284360335
1103	96-97	0.06518878901602676
1103	98-99	0.051707254984894035
1103	100	0.14659729480202088
1104	1	0.02336524535950346
1104	2	0.0034375249038589573
1104	3	0.035363167039619725
1104	4	0.06305211128726285
1104	5	-0.02812030692464873
1104	6	-0.029617095368458024
1104	7	0.04210268164705866
1104	8	0.08369650718704236
1104	9	0.09810604392922073
1104	10-11	0.11762976400130754
1104	12-13	0.13669821093692747
1104	14-15	0.1813444368807069
1104	16-17	0.21744278712752418
1104	18-19	0.24302367494330923
1104	20-21	0.1525901053420995
1104	22-23	0.103331295197016
1104	24-25	0.23764644627051723
1104	26-27	0.18228538939230532
1104	28-29	0.18705168333561062
1104	30-31	0.26151786765765905
1104	32-33	0.17270784371769565
1104	34-35	0.1923008013486438
1104	36-37	0.2524845580279518
1104	38-39	0.19791028616619144
1104	40-41	0.1326679471683292
1104	42-43	0.18364971245173223
1104	44-45	0.15585922176165212
1104	46-47	0.1285608381200234
1104	48-49	0.11328717300171576
1104	50-51	0.14896978518618909
1104	52-53	0.13303865188495223
1104	54-55	0.008838112696430755
1104	56-57	0.06946531740750572
1104	58-59	0.07335268270150408
1104	60-61	0.07857844848147266
1104	62-63	0.05009360576332966
1104	64-65	0.15050176289209816
1104	66-67	0.14070504568472586
1104	68-69	0.16527422135026626
1104	70-71	0.13131448996184503
1104	72-73	0.14260651070894426
1104	74-75	0.20042425383923756
1104	76-77	0.10880241593910256
1104	78-79	0.10362011099888235
1104	80-81	0.062213839773555435
1104	82-83	0.04117970277223293
1104	84-85	0.0182696074417521
1104	86-87	0.056028776197294405
1104	88-89	0.05418123447088874
1104	90-91	0.035024297579035135
1104	92-93	0.06150176178121569
1104	94-95	0.05445875732218752
1104	96-97	0.009911362950461466
1104	98-99	0.027021749082003055
1104	100	0.0828958457817599
1105	1	0.12375176114597508
1105	2	0.03408618252661455
1105	3	0.06259687320136109
1105	4	0.1348176116515063
1105	5	0.10130901214702703
1105	6	0.1378403078386441
1105	7	0.19970753631369575
1105	8	0.24850874098478215
1105	9	0.3117185840091139
1105	10-11	0.2966813409939846
1105	12-13	0.27921899457966504
1105	14-15	0.32174594182264116
1105	16-17	0.2508971109700866
1105	18-19	0.32286665807475146
1105	20-21	0.3321520048822606
1105	22-23	0.3527771041121639
1105	24-25	0.36655200159302836
1105	26-27	0.29450237824408276
1105	28-29	0.29740855757972895
1105	30-31	0.36780078752387624
1105	32-33	0.36199748063982184
1105	34-35	0.3896003900774119
1105	36-37	0.33849787988358315
1105	38-39	0.32907369750182625
1105	40-41	0.3257762738007557
1105	42-43	0.351514280676021
1105	44-45	0.26228320039280106
1105	46-47	0.2658429798735966
1105	48-49	0.3187746395981179
1105	50-51	0.28219147284574575
1105	52-53	0.24800169301148145
1105	54-55	0.17169543448824953
1105	56-57	0.2263094886820909
1105	58-59	0.2626139306240276
1105	60-61	0.21637735274289582
1105	62-63	0.19498305409505434
1105	64-65	0.17591807088054878
1105	66-67	0.25772030720914785
1105	68-69	0.2258019613318467
1105	70-71	0.1639095517529725
1105	72-73	0.11354240828875817
1105	74-75	0.20453150103977435
1105	76-77	0.14697669899131682
1105	78-79	0.1585865907097599
1105	80-81	0.15999946826346445
1105	82-83	0.0948783669826554
1105	84-85	0.1627708856852621
1105	86-87	0.16885209618001795
1105	88-89	0.0947921562891807
1105	90-91	0.09341367530752365
1105	92-93	0.1584725875791051
1105	94-95	0.16555971285869475
1105	96-97	0.1312705254483859
1105	98-99	0.16887333812745098
1105	100	0.11416025814719077
1106	1	0.055769006321025216
1106	2	0.04605505207855387
1106	3	0.090738062322675
1106	4	0.07189435844109227
1106	5	0.08890296235849604
1106	6	0.13084408437926243
1106	7	0.19809881128577445
1106	8	0.12695957339113306
1106	9	0.23945880874386916
1106	10-11	0.21716343602955135
1106	12-13	0.23798098633531595
1106	14-15	0.2312917767542828
1106	16-17	0.2824783592871185
1106	18-19	0.2534891353014146
1106	20-21	0.28816666046773776
1106	22-23	0.23829075293274116
1106	24-25	0.25352075950547714
1106	26-27	0.1834108164478323
1106	28-29	0.2218383365648009
1106	30-31	0.16793277889102143
1106	32-33	0.1565521306254638
1106	34-35	0.19008689263998235
1106	36-37	0.23697817618371175
1106	38-39	0.17303858956206852
1106	40-41	0.16138483851436547
1106	42-43	0.16498886549992875
1106	44-45	0.1684048289360689
1106	46-47	0.10813043280080592
1106	48-49	0.1352050473367825
1106	50-51	0.1724372404811092
1106	52-53	0.08869253379526754
1106	54-55	0.08147599144426465
1106	56-57	0.07190431727305224
1106	58-59	0.016134481890887287
1106	60-61	0.017210440097223056
1106	62-63	0.08889874663275776
1106	64-65	0.12347625849829313
1106	66-67	0.08442277591548475
1106	68-69	0.07491036372164928
1106	70-71	0.08299961400761902
1106	72-73	0.08600753322897958
1106	74-75	0.06615558319572301
1106	76-77	0.004477879020704734
1106	78-79	0.07556700155200247
1106	80-81	0.0718228045783178
1106	82-83	0.0934652901581714
1106	84-85	0.135159258477195
1106	86-87	-0.018397133619153294
1106	88-89	-0.03981743595429066
1106	90-91	0.039873051260649106
1106	92-93	0.03709780086634851
1106	94-95	0.007180622220381849
1106	96-97	0.0024753968597934772
1106	98-99	0.08225638973481963
1106	100	0.14271976043155732
1107	1	0.030988018436591602
1107	2	0.07343001324661813
1107	3	0.0303031446463784
1107	4	0.0020186206343169033
1107	5	-0.06569163223610275
1107	6	0.002412894568756485
1107	7	0.07594907534293327
1107	8	0.09758088435727075
1107	9	0.13320220731485222
1107	10-11	0.13454771686210876
1107	12-13	0.1801602345490423
1107	14-15	0.17395254096676638
1107	16-17	0.1038763089609347
1107	18-19	0.09138755095844431
1107	20-21	0.25006321219187555
1107	22-23	0.20187105737511502
1107	24-25	0.2676353913787253
1107	26-27	0.21132942471126626
1107	28-29	0.2783589951544201
1107	30-31	0.2229017133427078
1107	32-33	0.25153815112872735
1107	34-35	0.26297288207769753
1107	36-37	0.23428168659067694
1107	38-39	0.22099913631572576
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2216	22-23	-0.3207422365262005
2216	24-25	-0.36133208395937544
2216	26-27	-0.4328783676134478
2216	28-29	-0.40742182405023186
2216	30-31	-0.37913205144710105
2216	32-33	-0.3786372673300491
2216	34-35	-0.3653388784313236
2216	36-37	-0.3563039643864556
2216	38-39	-0.27370581001667205
2216	40-41	-0.32194052161210607
2216	42-43	-0.28454869610575173
2216	44-45	-0.2133649300939382
2216	46-47	-0.2189059376141529
2216	48-49	-0.10162903325468164
2216	50-51	-0.17670641611581317
2216	52-53	-0.10499806520531507
2216	54-55	-0.11829202975209085
2216	56-57	-0.14444970022864823
2216	58-59	-0.10470622776131933
2216	60-61	-0.08804471833798999
2216	62-63	-0.08008194448962058
2216	64-65	-0.16483370784908402
2216	66-67	-0.0983660100481174
2216	68-69	-0.1600895295228426
2216	70-71	-0.09180737294868635
2216	72-73	-0.027344551359348657
2216	74-75	-0.026814320328320207
2216	76-77	-0.13092768058709936
2216	78-79	-0.09163100947428404
2216	80-81	-0.14905839917123131
2216	82-83	-0.09465805478931344
2216	84-85	-0.04285913029569599
2216	86-87	-0.07048300847870337
2216	88-89	-0.08165060775528588
2216	90-91	-0.01733412249041777
2216	92-93	-0.01956829582932329
2216	94-95	-0.08804440013896908
2216	96-97	-0.04881770430738186
2216	98-99	-0.11901172881890787
2216	100	-0.1720310129071585
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	863.0
1	206.0
2	143.0
3	133.0
4	122.0
5	150.0
6	207.0
7	343.0
8	934.0
9	1628.0
10	2246.0
11	3443.0
12	4993.0
13	6593.0
14	8167.0
15	9227.0
16	9859.0
17	10556.0
18	11704.0
19	13191.0
20	14691.0
21	15899.0
22	17452.0
23	19991.0
24	23337.0
25	28350.0
26	31021.0
27	28300.0
28	30129.0
29	37479.0
30	43263.0
31	49112.0
32	62521.0
33	82916.0
34	119587.0
35	182458.0
36	331116.0
37	653908.0
38	795163.0
39	229332.0
40	422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67234431063665	19.630777119701616	13.282719988438119	24.414158581223614
2	14.856483184219766	21.013916687417886	40.26670612002922	23.862894008333132
3	17.928629362103432	26.181773341692967	30.418820979431487	25.470776316772113
4	12.186469320374522	17.777909002332517	39.342831289414406	30.692790387878553
5	12.963512949757492	38.63700072632993	35.41283919277686	12.986647131135726
6	29.91820832993186	38.5614462309655	18.446392499274836	13.073952939827802
7	26.090738341946413	33.340662875452324	23.138426566151203	17.430172216450064
8	24.68666542537079	36.44022048749579	20.9980366219337	17.875077465199716
9	25.7744043657845	16.4693954672198	20.488511056866066	37.26768911012964
10-11	23.827383550293003	27.055596823038684	29.16456879841343	19.952450828254886
12-13	24.50524012538096	24.90849100018234	28.466948571230105	22.119320303206592
14-15	22.021049472564844	26.330197905195224	25.813157057908885	25.835595564331044
16-17	22.083332392593732	29.032138732981807	26.553813807544824	22.330715066879637
18-19	21.97790689495572	28.68282821616543	27.642293507794935	21.69697138108392
20-21	22.991487708606883	27.835264056082448	27.21705188213558	21.956196353175088
22-23	22.808376715307272	27.801923255933502	26.935707845155875	22.45399218360335
24-25	22.491516601826568	27.941923000841783	27.197065097652644	22.369495299679006
26-27	22.38361784369522	28.047497930817944	26.970099233907703	22.598784991579134
28-29	22.418297622763063	28.292084207579293	26.923163093525517	22.366455076132123
30-31	22.645033917972643	28.003234699656993	26.933946231107686	22.417785151262677
32-33	22.360645122855026	28.242457590364666	26.710189202666378	22.686708084113928
34-35	22.69886010333896	28.224183845245843	26.87801222920767	22.19894382220752
36-37	22.72536764099237	27.93135341734819	26.840671389354238	22.5026075523052
38-39	22.519893774806967	28.08499196571669	26.96007071489402	22.435043544582324
40-41	22.647562315976984	27.8060948005986	27.0953960029402	22.450946880484217
42-43	22.467806346688	27.917603840675003	26.90515193986986	22.709437872767133
44-45	22.502528961472244	28.042899940666672	26.997393224328608	22.457177873532473
46-47	22.515333928268095	27.965083419974036	26.840166919843945	22.679415731913917
48-49	22.439197329712922	27.934574770400612	26.87851833529501	22.74770956459146
50-51	22.478694014522723	27.983432875615428	26.87705884038814	22.660814269473708
52-53	22.519326164229533	27.88666963241769	26.89426634401241	22.69973785934037
54-55	22.28300120369132	27.955704854193552	26.99714622678378	22.764147715331344
56-57	22.42714515523034	27.944014211664804	26.95295312926386	22.675887503841004
58-59	22.420360258168518	27.80519411712082	26.97150540109316	22.802940223617505
60-61	22.321274870113548	27.916419638202676	27.00430919550393	22.757996296179847
62-63	22.326757130280527	27.78839532816974	27.057127709617774	22.827719831931955
64-65	22.183619423500016	27.79278172057113	26.973883180017495	23.04971567591136
66-67	22.27461880214192	27.77990193736673	26.907187930218317	23.03829133027304
68-69	22.34281608756134	27.684595469345613	26.986643032739615	22.98594541035344
70-71	22.26139687316568	27.545788220285143	27.00350226706693	23.189312639482246
72-73	22.400835479240175	27.556605386145105	27.006376574340557	23.036182560274156
74-75	22.27133777457907	27.696411686768315	27.033525446830215	22.9987250918224
76-77	22.35791854456893	27.53631672614865	26.97515653648957	23.130608192792852
78-79	22.30152549154023	27.55366651477361	27.001691902166048	23.14311609152011
80-81	22.37791648064539	27.568387790518905	27.01315554042293	23.04054018841277
82-83	22.46513625325862	27.398977045188673	27.201956963072938	22.933929738479772
84-85	22.4211961586922	27.57222863914856	26.964200499715364	23.042374702443873
86-87	22.307450982027092	27.57763871575621	27.07455220593038	23.04035809628632
88-89	22.433179432117893	27.30685495897579	26.97484315295351	23.285122455952806
90-91	22.4635383887667	27.309307757903827	26.988288891270667	23.2388649620588
92-93	22.516621976044064	27.2396307304498	27.07018445586207	23.173562837644067
94-95	22.258886740394832	27.09624658937183	27.257563135269663	23.38730353496367
96-97	22.362687878194674	26.9982340131465	27.18765160572446	23.451426502934368
98-99	22.226366413359496	27.16514395148065	27.062214751482877	23.546274883676972
100	22.50418302095355	27.03858121393025	26.947457638323257	23.50977812679294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2092.0
1	1497.5
2	973.5
3	1018.0
4	931.5
5	807.5
6	732.0
7	697.5
8	661.0
9	643.0
10	599.0
11	598.5
12	624.0
13	634.5
14	668.5
15	749.5
16	893.0
17	1087.0
18	1362.0
19	1687.5
20	2245.5
21	3111.0
22	4352.5
23	6255.5
24	8633.0
25	11549.5
26	15268.0
27	19540.0
28	23961.5
29	29075.5
30	35674.0
31	42573.0
32	50317.0
33	56414.5
34	60562.5
35	65607.0
36	71480.5
37	78304.0
38	82930.5
39	85190.5
40	87666.0
41	90177.0
42	92657.0
43	96452.0
44	100608.5
45	105914.5
46	110735.5
47	113174.5
48	114682.0
49	113988.0
50	110955.5
51	107943.0
52	104701.5
53	101359.0
54	97737.0
55	90387.0
56	81587.0
57	72084.5
58	62917.5
59	54487.0
60	45276.0
61	37075.5
62	29976.5
63	23082.5
64	16591.5
65	11863.5
66	8797.5
67	6660.0
68	4880.0
69	3568.0
70	2843.5
71	2243.5
72	1774.5
73	1418.0
74	1098.5
75	838.5
76	597.0
77	404.0
78	282.5
79	186.0
80	122.0
81	89.5
82	67.5
83	54.0
84	36.0
85	29.0
86	24.0
87	17.0
88	13.0
89	12.5
90	9.5
91	5.0
92	5.5
93	7.0
94	5.5
95	3.5
96	3.5
97	5.0
98	4.0
99	5.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09478837480107805
2	0.06268319476043462
3	0.07774659815247704
4	0.07948201328980913
5	0.07975967971178224
6	0.08677075686660385
7	0.08309167677545984
8	0.08479238361004528
9	0.0707702293004021
10-11	0.07776395230385037
12-13	0.06698702430101816
14-15	0.07550791262531867
16-17	0.07686153643243768
18-19	0.07644503679947799
20-21	0.06821916904852394
22-23	0.07215856141026775
24-25	0.07460549675390599
26-27	0.06934718888778979
28-29	0.07793749381758357
30-31	0.08231073996366041
32-33	0.08387261358725927
34-35	0.08583363269244453
36-37	0.08668398610973724
38-39	0.08862765106354917
40-41	0.08465355039905871
42-43	0.09018952468714804
44-45	0.08687488177484377
46-47	0.08357759301391282
48-49	0.09242821021430642
50-51	0.08894002578826894
52-53	0.09504868707167785
54-55	0.08711783989407026
56-57	0.09282735569589279
58-59	0.09727001844746291
60-61	0.09633289427330359
62-63	0.09707912278235638
64-65	0.09848480904359536
66-67	0.10039376569466063
68-69	0.0974262058098228
70-71	0.09983843285071438
72-73	0.0974262058098228
74-75	0.10094909853860692
76-77	0.09831126752986215
78-79	0.1052876363819371
80-81	0.10065407796526046
82-83	0.10488849090035074
84-85	0.10122676496058004
86-87	0.09798153865376905
88-89	0.09695764372274314
90-91	0.09786005959415581
92-93	0.09237614776018646
94-95	0.09208112718684
96-97	0.09005069147616147
98-99	0.10284070103829888
100	0.24420761812536987
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2881155.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.00426679619036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17357907698339	49.61776969421725
2	14.591175119953812	19.261596309747326
3	5.163315288900954	10.224025196444018
4	2.1733026463993665	5.737889908072414
5	1.0997997731897564	3.629573882600316
6	0.5855418092861441	2.3188954680268004
7	0.35400923989339456	1.6356284222768123
8	0.21898486394367256	1.1563148307252489
9	0.14345906100421357	0.8522019123167756
>10	0.4857171966317404	4.915688951740225
>50	0.009589890826461042	0.4196505060421398
>100	0.0014535321656686752	0.12960233210276925
>500	0.0	0.0
>1k	7.250082144190267E-5	0.10116258568787559
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.470830274664154E-5	6.941660549328308E-5
2	0.0	0.0	0.0	3.470830274664154E-5	6.941660549328308E-5
3	0.0	0.0	0.0	3.470830274664154E-5	6.941660549328308E-5
4	0.0	0.0	0.0	3.470830274664154E-5	6.941660549328308E-5
5	0.0	0.0	0.0	6.941660549328308E-5	6.941660549328308E-5
6	0.0	0.0	0.0	6.941660549328308E-5	1.0412490823992461E-4
7	0.0	0.0	0.0	6.941660549328308E-5	1.0412490823992461E-4
8	0.0	0.0	0.0	6.941660549328308E-5	1.0412490823992461E-4
9	0.0	0.0	0.0	6.941660549328308E-5	1.0412490823992461E-4
10-11	0.0	0.0	0.0	1.0412490823992461E-4	1.0412490823992461E-4
12-13	0.0	0.0	0.0	1.0412490823992461E-4	2.9502057334645306E-4
14-15	0.0	0.0	0.0	1.3883321098656615E-4	3.817913302130569E-4
16-17	0.0	0.0	0.0	1.7354151373320768E-4	4.5120793570634E-4
18-19	0.0	0.0	0.0	1.7354151373320768E-4	5.900411466929061E-4
20-21	0.0	0.0	0.0	1.9089566510652845E-4	6.073952980662269E-4
22-23	0.0	0.0	0.0	3.123747247197738E-4	6.247494494395477E-4
24-25	0.0	0.0	0.0	6.247494494395476E-4	6.247494494395477E-4
26-27	0.0	0.0	0.0	0.00112801983926585	6.7681190355951E-4
28-29	0.0	0.0	0.0	0.0042170587837169465	6.941660549328307E-4
30-31	0.0	0.0	0.0	0.01376184203904337	6.941660549328307E-4
32-33	0.0	0.0	0.0	0.034864490109001424	6.941660549328307E-4
34-35	0.0	0.0	0.0	0.06863566868148364	7.288743576794723E-4
36-37	0.0	0.0	0.0	0.12127080979676552	7.288743576794723E-4
38-39	0.0	0.0	0.0	0.20016278193988174	7.982909631727554E-4
40-41	0.0	0.0	0.0	0.2875235799531785	7.982909631727554E-4
42-43	0.0	0.0	0.0	0.37439846172802227	8.677075686660384E-4
44-45	0.0	0.0	0.0	0.4905845051724048	9.0241587141268E-4
46-47	0.0	0.0	0.0	0.6346066074195938	9.371241741593215E-4
48-49	0.0	0.0	0.0	0.7885552842523225	0.0010586032337725668
50-51	0.0	0.0	0.0	0.9577061976880799	0.0010759573851458876
52-53	0.0	0.0	0.0	1.1192733469736962	0.0011106656878925292
54-55	0.0	0.0	0.0	1.299739167104859	0.0011453739906391708
56-57	0.0	0.0	0.0	1.5197030357616996	0.0011800822933858124
58-59	0.0	0.0	0.0	1.765715485629895	0.0012494988988790954
60-61	0.0	0.0	0.0	2.0335768120771007	0.0012494988988790954
62-63	0.0	0.0	0.0	2.3042148027440383	0.0013189155043723784
64-65	0.0	0.0	0.0	2.5848834929047553	0.0013189155043723784
66-67	0.0	0.0	0.0	2.9022562132200456	0.0013189155043723784
68-69	0.0	0.0	0.0	3.2616780423128917	0.0013189155043723784
70-71	1.735415137332077E-5	0.0	0.0	3.6576824225006987	0.0013883321098656616
72-73	3.470830274664154E-5	0.0	0.0	4.064793459567431	0.0015098111694789068
74-75	3.470830274664154E-5	0.0	0.0	4.468988999203445	0.0016312902290921525
76-77	3.470830274664154E-5	0.0	0.0	4.9073201545907805	0.0016659985318387938
78-79	3.470830274664154E-5	0.0	0.0	5.399188866964812	0.0017007068345854354
80-81	3.470830274664154E-5	0.0	0.0	5.929028462543668	0.0017354151373320768
82-83	3.470830274664154E-5	0.0	0.0	6.482157329265521	0.001874248348318643
84-85	3.470830274664154E-5	0.0	0.0	7.029906409061644	0.0018916024996919638
86-87	3.470830274664154E-5	0.0	0.0	7.583295588054097	0.0020130815593052092
88	3.470830274664154E-5	0.0	0.0	8.020221057180194	0.0021172064675451336
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2565	0.0	40.884857	1
GTATCAA	4550	0.0	37.311337	1
CAACGCA	4730	0.0	33.59014	5
ATCAACG	4830	0.0	32.800797	3
TCAACGC	4880	0.0	32.560486	4
AACGCAG	5120	0.0	31.300962	6
TATCAAC	5635	0.0	29.032667	2
ACGCAGA	5980	0.0	26.563715	7
CGCAGAG	6165	0.0	25.309193	8
GTACATG	8420	0.0	24.462847	1
TACATGG	8155	0.0	23.750614	2
ACATGGG	8045	0.0	22.965086	3
GCAGAGT	7200	0.0	21.08243	9
CATGGGG	5625	0.0	20.39207	4
TATACTG	1490	0.0	17.035849	5
GAGTACA	6370	0.0	16.979834	1
AGAGTAC	6095	0.0	16.885632	10-11
CAGAGTA	6870	0.0	16.656706	10-11
GTATTAT	1445	0.0	16.272232	1
ATGGGGG	3625	0.0	16.079405	5
>>END_MODULE