FastQCFastQC Report
Mon 16 Jan 2017
ERR1146511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881155
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118350.41077276300650256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106950.37120529787533124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62960.2185234740928551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56800.064.1683651
GTATCAA110750.044.3327181
ATCAACG143400.033.906753
CAACGCA144600.033.6740345
TATCAAC146050.033.5186582
TCAACGC146300.033.395354
AACGCAG150550.032.4357156
ACGCAGA168100.028.963977
CGCAGAG170550.028.3825578
GCAGAGT183200.026.1662039
TGGTATC19150.025.7844562
GTGGTAT20700.025.4457511
GTACATG80900.024.764451
TACATGG79700.024.191512
ACATGGG77300.023.6029913
GAGTACT138000.023.022512-13
AGAGTAC169900.022.59987310-11
CAGAGTA179950.022.52602610-11
TAACGCC4200.021.2725284
CTAACGC3800.021.0368213
Produced by FastQC (version 0.11.5)