##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2881155
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26254852654578	33.0	31.0	34.0	30.0	34.0
2	32.3939458307519	34.0	31.0	34.0	30.0	34.0
3	32.49022978631833	34.0	31.0	34.0	30.0	34.0
4	36.0053995706583	37.0	35.0	37.0	35.0	37.0
5	36.0524268912988	37.0	35.0	37.0	35.0	37.0
6	36.12370733264958	37.0	36.0	37.0	35.0	37.0
7	36.09045573736921	37.0	36.0	37.0	35.0	37.0
8	36.092638889611976	37.0	36.0	37.0	35.0	37.0
9	37.865507409354926	39.0	38.0	39.0	35.0	39.0
10-11	37.81254028332388	39.0	38.0	39.0	35.0	39.0
12-13	37.78886575002039	39.0	38.0	39.0	35.0	39.0
14-15	39.129777294175426	40.0	39.0	41.0	36.0	41.0
16-17	39.07544977621822	40.0	38.5	41.0	36.0	41.0
18-19	39.191075280573244	40.0	39.0	41.0	36.0	41.0
20-21	39.251740708153505	40.0	39.0	41.0	36.0	41.0
22-23	39.21122171490253	40.0	39.0	41.0	36.0	41.0
24-25	39.204778465580645	41.0	39.0	41.0	36.0	41.0
26-27	39.125636940740776	40.0	39.0	41.0	35.5	41.0
28-29	39.05714878234597	40.0	39.0	41.0	35.0	41.0
30-31	38.90100237578332	40.0	38.5	41.0	35.0	41.0
32-33	38.77777696791738	40.0	38.0	41.0	35.0	41.0
34-35	38.69371016137625	40.0	38.0	41.0	35.0	41.0
36-37	38.6100411119846	40.0	38.0	41.0	35.0	41.0
38-39	38.52086715223582	40.0	38.0	41.0	35.0	41.0
40-41	38.417723794797574	40.0	38.0	41.0	34.0	41.0
42-43	38.30869824775134	40.0	38.0	41.0	34.0	41.0
44-45	38.16430927874411	40.0	38.0	41.0	33.5	41.0
46-47	37.914329669871975	40.0	38.0	41.0	33.0	41.0
48-49	37.62758494423243	40.0	37.0	41.0	33.0	41.0
50-51	37.52655410764086	40.0	37.0	41.0	32.5	41.0
52-53	37.43105317138439	40.0	37.0	41.0	32.0	41.0
54-55	37.28357794009694	40.0	36.5	41.0	32.0	41.0
56-57	37.15102189920361	40.0	36.0	41.0	32.0	41.0
58-59	36.990639517832264	39.0	36.0	41.0	31.0	41.0
60-61	36.79942991612738	39.0	35.0	41.0	31.0	41.0
62-63	36.57279875605443	39.0	35.0	41.0	31.0	41.0
64-65	36.31143031180204	38.5	35.0	41.0	31.0	41.0
66-67	36.04661203579814	37.5	35.0	40.0	31.0	41.0
68-69	35.75893886306012	37.0	35.0	40.0	30.5	41.0
70-71	35.4457167351288	36.5	35.0	39.0	30.0	41.0
72-73	35.115379769571575	36.0	35.0	39.0	30.0	41.0
74-75	34.791419586936485	36.0	35.0	39.0	30.0	40.5
76-77	34.48621455631509	35.0	34.0	37.5	29.5	39.5
78-79	34.187214328975706	35.0	34.0	37.0	29.0	39.0
80-81	33.908350817640844	35.0	34.0	36.5	29.0	39.0
82-83	33.651744352525284	35.0	34.0	36.0	29.0	38.0
84-85	33.433160659527175	35.0	34.0	36.0	29.0	37.0
86-87	33.237278973189575	35.0	34.0	35.5	29.0	37.0
88-89	33.03080240389705	35.0	34.0	35.0	29.0	36.0
90-91	32.94211869892456	35.0	34.0	35.0	29.0	36.0
92-93	32.84437647401823	35.0	34.0	35.0	29.0	36.0
94-95	32.76270315203452	35.0	34.0	35.0	29.0	36.0
96-97	32.70562378629404	35.0	34.0	35.0	29.0	35.5
98-99	32.63154793824005	35.0	34.0	35.0	27.0	35.0
100	31.913577714492973	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3041340031120825
1101	2	0.3268440314550105
1101	3	0.34454129211279394
1101	4	0.26678595511875614
1101	5	0.2403372599828515
1101	6	0.2663531852771399
1101	7	0.2529659401432838
1101	8	0.2891546770542561
1101	9	0.3845578606813902
1101	10-11	0.38391813998175195
1101	12-13	0.40124664490319617
1101	14-15	0.5465393988450202
1101	16-17	0.5560303083848126
1101	18-19	0.4687288289094482
1101	20-21	0.45888195153963096
1101	22-23	0.4625219219498504
1101	24-25	0.47835868068374765
1101	26-27	0.4782715365081387
1101	28-29	0.4867208570136299
1101	30-31	0.5962242601988805
1101	32-33	0.46584389250958935
1101	34-35	0.5192336588658435
1101	36-37	0.48364343893909023
1101	38-39	0.5366689248915932
1101	40-41	0.5492093868764059
1101	42-43	0.5453498746938834
1101	44-45	0.5270647096881262
1101	46-47	0.5419469262600174
1101	48-49	0.6699325401759992
1101	50-51	0.5676865670693871
1101	52-53	0.5622143793485819
1101	54-55	0.5639860624598256
1101	56-57	0.46209909305559194
1101	58-59	0.4436517330194931
1101	60-61	0.46122552405503825
1101	62-63	0.4214985801447355
1101	64-65	0.4062303803625653
1101	66-67	0.4186829573858475
1101	68-69	0.3499044697374245
1101	70-71	0.3573378245090453
1101	72-73	0.34524559547081424
1101	74-75	0.35185660334654756
1101	76-77	0.3202645735543399
1101	78-79	0.2686375811788082
1101	80-81	0.26820194590852964
1101	82-83	0.24801732409159882
1101	84-85	0.24273830692006015
1101	86-87	0.28245528406176845
1101	88-89	0.3183440383539349
1101	90-91	0.2792673258650069
1101	92-93	0.2790053333987572
1101	94-95	0.3602250959001765
1101	96-97	0.3570097820653544
1101	98-99	0.45713179328685527
1101	100	0.42374033589894466
1102	1	0.2911688668765393
1102	2	0.3045571763573207
1102	3	0.27211130251887283
1102	4	0.18898855758880728
1102	5	0.20199166411597957
1102	6	0.19848687968023881
1102	7	0.24104120853742472
1102	8	0.23567834037478974
1102	9	0.28462834254943914
1102	10-11	0.29066031744410026
1102	12-13	0.36878760782526143
1102	14-15	0.4324411597385023
1102	16-17	0.5091116684745671
1102	18-19	0.4044466517998373
1102	20-21	0.40432101491089867
1102	22-23	0.4059965152375824
1102	24-25	0.38955773396098436
1102	26-27	0.3487710080637285
1102	28-29	0.360097603288402
1102	30-31	0.4229570694745419
1102	32-33	0.47548203357914787
1102	34-35	0.4424519057328311
1102	36-37	0.4980829283220416
1102	38-39	0.43001350999827537
1102	40-41	0.4896243276757062
1102	42-43	0.4660910468229531
1102	44-45	0.4681673405137303
1102	46-47	0.47592722525509856
1102	48-49	0.5491966592747488
1102	50-51	0.5718122662915732
1102	52-53	0.5090600613674923
1102	54-55	0.490021040189184
1102	56-57	0.46912275705448536
1102	58-59	0.49413031779242544
1102	60-61	0.44837835506542945
1102	62-63	0.3815772498486538
1102	64-65	0.42198741939147766
1102	66-67	0.40301332537039514
1102	68-69	0.39970913524363993
1102	70-71	0.2782749755763092
1102	72-73	0.3116904601985411
1102	74-75	0.38655467774663776
1102	76-77	0.36722310118200596
1102	78-79	0.4064153875985639
1102	80-81	0.3042867629338133
1102	82-83	0.2700680411886438
1102	84-85	0.25281317779375456
1102	86-87	0.2791577637482021
1102	88-89	0.3226337317204937
1102	90-91	0.2721993647921579
1102	92-93	0.31273440082663484
1102	94-95	0.34261643239648976
1102	96-97	0.34041286237398793
1102	98-99	0.32818935370521984
1102	100	0.32562170657116596
1103	1	0.2639186936959419
1103	2	0.2615649534209723
1103	3	0.27995745151675777
1103	4	0.1919719876104793
1103	5	0.16206800342272487
1103	6	0.19176330310097
1103	7	0.23400442787328046
1103	8	0.21648872565512534
1103	9	0.3087620061949323
1103	10-11	0.3016751122126138
1103	12-13	0.2920529614793921
1103	14-15	0.4218872454253386
1103	16-17	0.49667930299556673
1103	18-19	0.41304329836366094
1103	20-21	0.4183085417837802
1103	22-23	0.3887015274502872
1103	24-25	0.3332024589629654
1103	26-27	0.40714016599391556
1103	28-29	0.38062742215397094
1103	30-31	0.38438924090879567
1103	32-33	0.4176922709833306
1103	34-35	0.4259894865818481
1103	36-37	0.3963206011151499
1103	38-39	0.3673352825349028
1103	40-41	0.43986238533607036
1103	42-43	0.4697255997811567
1103	44-45	0.48871620296100815
1103	46-47	0.5197539824089503
1103	48-49	0.5519246926321486
1103	50-51	0.44716951067305644
1103	52-53	0.4302493423894731
1103	54-55	0.39291536815986916
1103	56-57	0.34936382624300677
1103	58-59	0.33764843358758867
1103	60-61	0.4180545929095203
1103	62-63	0.44428702327900993
1103	64-65	0.49616265346802635
1103	66-67	0.452688459911208
1103	68-69	0.37145757900278653
1103	70-71	0.42350129742804654
1103	72-73	0.3905599854990811
1103	74-75	0.32463970801562425
1103	76-77	0.29245419714045795
1103	78-79	0.28957315371617653
1103	80-81	0.30914732299298464
1103	82-83	0.2950258955834144
1103	84-85	0.2656925679918487
1103	86-87	0.2194574884572802
1103	88-89	0.3065619154416268
1103	90-91	0.31276564164433296
1103	92-93	0.34279068282734215
1103	94-95	0.27576901389092967
1103	96-97	0.2736458393614143
1103	98-99	0.30963777952924687
1103	100	0.27047065616779875
1104	1	0.24796005975471758
1104	2	0.17893439885096285
1104	3	0.1583752893110031
1104	4	0.1273017038873192
1104	5	0.1110743759472399
1104	6	0.16825601353518493
1104	7	0.14064419916033444
1104	8	0.13981187100394266
1104	9	0.2084689393703556
1104	10-11	0.22201763578875244
1104	12-13	0.24963916657833352
1104	14-15	0.3631779292612194
1104	16-17	0.4168202645503598
1104	18-19	0.31100486327978416
1104	20-21	0.2862466877259706
1104	22-23	0.2954479521239577
1104	24-25	0.26114365302091613
1104	26-27	0.2672753195695847
1104	28-29	0.3077066802168318
1104	30-31	0.3290615239996342
1104	32-33	0.3140306375919337
1104	34-35	0.32958059013162
1104	36-37	0.26445957311511137
1104	38-39	0.3103856859195986
1104	40-41	0.2941832734233003
1104	42-43	0.27370007901793514
1104	44-45	0.21906911895597858
1104	46-47	0.29407974012178073
1104	48-49	0.3490109515484221
1104	50-51	0.3063817659383403
1104	52-53	0.28015809824000115
1104	54-55	0.2964259756947243
1104	56-57	0.3452033015500433
1104	58-59	0.3285659685634883
1104	60-61	0.2450116196903167
1104	62-63	0.3135200971136385
1104	64-65	0.22882045402251805
1104	66-67	0.19012536253494972
1104	68-69	0.1830835647723177
1104	70-71	0.21098902753548998
1104	72-73	0.1874380280567891
1104	74-75	0.1980323865887499
1104	76-77	0.06304205723746747
1104	78-79	0.12403845012090642
1104	80-81	0.19294226391810554
1104	82-83	0.14558932169006766
1104	84-85	0.16051659757022207
1104	86-87	0.11096768875896146
1104	88-89	0.22351443751210098
1104	90-91	0.14723541516074334
1104	92-93	0.16860481952215167
1104	94-95	0.17949740531070546
1104	96-97	0.13571182401472726
1104	98-99	0.148096506232541
1104	100	0.22122776335261207
1105	1	0.209724833407833
1105	2	0.17967682584160372
1105	3	0.1320009338240098
1105	4	0.08474366891420004
1105	5	0.09330752969036382
1105	6	0.07773616693622643
1105	7	0.15875508109813552
1105	8	0.15943053081864633
1105	9	0.19469929970681932
1105	10-11	0.1694758306974009
1105	12-13	0.15963903659747558
1105	14-15	0.33287233246300474
1105	16-17	0.40426727519756867
1105	18-19	0.3581328182024279
1105	20-21	0.24502184271336347
1105	22-23	0.29523132721855916
1105	24-25	0.26950024012551665
1105	26-27	0.2645470214974921
1105	28-29	0.24607513693646155
1105	30-31	0.280030708493868
1105	32-33	0.26111209481907593
1105	34-35	0.22013866291735695
1105	36-37	0.2019305415965249
1105	38-39	0.22617865478268584
1105	40-41	0.21752659361872162
1105	42-43	0.25033755710582284
1105	44-45	0.2189186070382121
1105	46-47	0.19662491289882666
1105	48-49	0.272056822381721
1105	50-51	0.20842385373804007
1105	52-53	0.10662931900243677
1105	54-55	0.15083578286813548
1105	56-57	0.13172626650780472
1105	58-59	0.13119604246941918
1105	60-61	0.13227317129536686
1105	62-63	0.10224833492187457
1105	64-65	0.051546749207851406
1105	66-67	0.06442297850888679
1105	68-69	0.07124080260832955
1105	70-71	0.05800833028275321
1105	72-73	0.06677754217654552
1105	74-75	0.09517945460477506
1105	76-77	0.03324288356118643
1105	78-79	0.014035419203779043
1105	80-81	0.05622403227214079
1105	82-83	0.06393009248832726
1105	84-85	0.013668485512546624
1105	86-87	0.028593180839877164
1105	88-89	0.07922390549456537
1105	90-91	0.06276257318260292
1105	92-93	0.07309577610440954
1105	94-95	0.1107076392939561
1105	96-97	0.1157155698265413
1105	98-99	0.1224134420875842
1105	100	0.09460585342579364
1106	1	0.14738846898796254
1106	2	0.13508388351144163
1106	3	0.1279698551766657
1106	4	0.0704223320625843
1106	5	0.11430367991424362
1106	6	0.11784823512026321
1106	7	0.11534987128511176
1106	8	0.1249053491923604
1106	9	0.11740357875909524
1106	10-11	0.12740767121726293
1106	12-13	0.20181509564316258
1106	14-15	0.2762780334897741
1106	16-17	0.30957678228242713
1106	18-19	0.2567056078187022
1106	20-21	0.24801612543188156
1106	22-23	0.25332778016407786
1106	24-25	0.24081592489310566
1106	26-27	0.19927615730514248
1106	28-29	0.17196309509933627
1106	30-31	0.25414072965624257
1106	32-33	0.23617613122961956
1106	34-35	0.20899056688416806
1106	36-37	0.22681068544177663
1106	38-39	0.28368309468513786
1106	40-41	0.2694426956500635
1106	42-43	0.2838536293964111
1106	44-45	0.3189404995418883
1106	46-47	0.30169402667330303
1106	48-49	0.3804844985253908
1106	50-51	0.3391411910379958
1106	52-53	0.26352315788700764
1106	54-55	0.31662996832633183
1106	56-57	0.33132053511509696
1106	58-59	0.286206858103057
1106	60-61	0.29403940401189743
1106	62-63	0.35189605651621747
1106	64-65	0.31087484248130437
1106	66-67	0.2844623448252861
1106	68-69	0.17639805575890222
1106	70-71	0.2593566785318657
1106	72-73	0.2289204465269563
1106	74-75	0.27710655089293823
1106	76-77	0.2541637552780429
1106	78-79	0.2711742621096107
1106	80-81	0.2561498143402474
1106	82-83	0.20304835711053926
1106	84-85	0.16429393458238906
1106	86-87	0.14805937885886067
1106	88-89	0.280766394814016
1106	90-91	0.22468800380959664
1106	92-93	0.17203376081516097
1106	94-95	0.22544383964844172
1106	96-97	0.2212029967640845
1106	98-99	0.20189277560877628
1106	100	0.34054452047709916
1107	1	0.10275348918057148
1107	2	0.08496583442786942
1107	3	0.08836749419499768
1107	4	0.08230801084481953
1107	5	0.06683241554642905
1107	6	0.12060064270026061
1107	7	0.12020852304031848
1107	8	0.10299171141665653
1107	9	0.13181488661868457
1107	10-11	0.15221506481776004
1107	12-13	0.18613320685782497
1107	14-15	0.2354889685627768
1107	16-17	0.2969393172311712
1107	18-19	0.21849653667860025
1107	20-21	0.17198630250550906
1107	22-23	0.1863815235626518
1107	24-25	0.20394422560762138
1107	26-27	0.21993940676149748
1107	28-29	0.22594725166970164
1107	30-31	0.23032277376933052
1107	32-33	0.21536998025106158
1107	34-35	0.16914278807087868
1107	36-37	0.12423379820971547
1107	38-39	0.10623202292372724
1107	40-41	0.1312398376196242
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2216	54-55	-0.4751217681842874
2216	56-57	-0.5445696871936718
2216	58-59	-0.5647100576445894
2216	60-61	-0.6242548811377162
2216	62-63	-0.6554275309213793
2216	64-65	-0.5673134517865108
2216	66-67	-0.5156191041516962
2216	68-69	-0.45223480219915757
2216	70-71	-0.46054285358702884
2216	72-73	-0.4255877335909304
2216	74-75	-0.42223862269059964
2216	76-77	-0.46042318485548606
2216	78-79	-0.4299936476960369
2216	80-81	-0.5193850535593896
2216	82-83	-0.5219860692592775
2216	84-85	-0.41664986986633323
2216	86-87	-0.3763325207887789
2216	88-89	-0.3644874515321419
2216	90-91	-0.37788631399077843
2216	92-93	-0.3870813026423221
2216	94-95	-0.38334460670490955
2216	96-97	-0.4317248825003688
2216	98-99	-0.40617990700747697
2216	100	-0.506181945088958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	14.0
8	100.0
9	282.0
10	343.0
11	201.0
12	209.0
13	273.0
14	472.0
15	878.0
16	1574.0
17	2833.0
18	4541.0
19	6838.0
20	9780.0
21	14287.0
22	19876.0
23	23131.0
24	21738.0
25	19075.0
26	17699.0
27	19433.0
28	23274.0
29	28751.0
30	35507.0
31	44572.0
32	57153.0
33	74409.0
34	105463.0
35	162536.0
36	289145.0
37	644735.0
38	934301.0
39	316841.0
40	889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88173673218703	19.386488928318425	13.697285991512237	24.034488347982307
2	15.316267475254019	20.998558176568878	39.86582781282258	23.819346535354523
3	17.85649703434701	26.286871957164372	31.041183859378517	24.815447149110096
4	12.194363070905407	17.992036736853947	38.86487038341174	30.9487298088289
5	12.500013015893622	38.298104226416854	35.5184994028308	13.68338335485873
6	29.545563406827064	38.793785069533385	17.981737289929896	13.678914233709651
7	25.415857673696024	33.79944286213094	23.078738588773803	17.70596087539923
8	25.225413776323723	35.24934175498222	20.837125227253324	18.68811924144073
9	26.080348360413918	15.132894666512097	20.775113027627125	38.01164394544686
10-11	24.066410276033828	26.365734799344914	29.04122849535281	20.526626429268447
12-13	25.285866258497023	24.42641926588469	28.816412168036777	21.471302307581507
14-15	22.39164501736283	25.358857819173213	27.186961478990195	25.062535684473765
16-17	21.37969704426744	28.265149261734994	27.83434861185493	22.520805082142633
18-19	21.188171389896397	26.926071186852475	30.528313347100983	21.35744407615015
20-21	22.12310088787494	26.08341324936683	30.53052792479667	21.26295793796156
22-23	21.899828367442918	26.068798797704396	30.310396351463215	21.720976483389475
24-25	21.626396358404875	26.19499818649118	30.56210096298186	21.616504492122083
26-27	21.589488216066687	26.335409762950352	30.314848510879777	21.76025351010318
28-29	21.539178131544144	26.523881312161496	30.33471478186956	21.602225774424802
30-31	21.804158061885012	26.288009996008537	30.231626260347433	21.67620568175902
32-33	21.715884877873375	26.44148220033049	29.94086734146689	21.901765580329236
34-35	21.96610557754232	26.44827026684634	30.0904933008579	21.495130854753437
36-37	22.059004471992044	26.217754028810326	29.939942477919875	21.783299021277756
38-39	21.90144575018031	26.374007595620334	29.93286671151012	21.791679942689232
40-41	22.041605059108544	26.24159186988484	29.921055554398606	21.79574751660801
42-43	22.00811827201244	26.30082380156569	29.61631706728725	22.074740859134618
44-45	22.080366549797276	26.501746793322706	29.470210800494872	21.947675856385146
46-47	22.266660419172172	26.550324435859924	28.93867216446182	22.244342980506083
48-49	22.377441797142566	26.747500088072425	28.58804891030548	22.287009204479528
50-51	22.37656425981941	26.953357247353928	28.371937643063283	22.298140849763374
52-53	22.385498272996532	26.949132923299807	28.369800008434144	22.29556879526952
54-55	22.208894199273033	26.927066385875765	28.348965125874326	22.515074288976873
56-57	22.306870120113476	26.934257686295805	28.288022441035277	22.47084975255545
58-59	22.15436315104345	26.863410923717723	28.414397175834726	22.567828749404097
60-61	22.153364399761482	26.907284552145715	28.482634292421956	22.45671675567085
62-63	22.09621158218073	26.777779706020166	28.521892299949673	22.604116411849436
64-65	21.934553125735384	26.722769342476916	28.498173990488507	22.84450354129919
66-67	21.940893268502222	26.80697518714031	28.429499983860484	22.822631560496987
68-69	22.021559216518206	26.72002729524402	28.442709837394766	22.815703650843012
70-71	21.99522778899184	26.57565138042338	28.384225289733322	23.04489554085145
72-73	22.07147763555819	26.63599865188928	28.325666618883478	22.966857093669052
74-75	22.03181310322494	26.76834045046349	28.193536653133535	23.006309793178037
76-77	22.060418648740676	26.695183078557644	28.125920894290967	23.118477378410716
78-79	21.96103911655205	26.730641776350556	28.08318846782007	23.22513063927732
80-81	22.040169399194447	26.765339979351804	28.031339163350687	23.163151458103062
82-83	22.108624956115364	26.641320160971365	28.16493925047208	23.08511563244119
84-85	22.056277048764393	26.65199422867137	27.917656871119373	23.374071851444864
86-87	21.87558461797439	26.839427243588077	27.93400563315754	23.35098250528
88-89	21.980750775296713	26.556762826019426	27.862992445737905	23.599493952945956
90-91	21.873023795326457	26.53889726130571	27.916539692081717	23.671539251286116
92-93	21.93050457047846	26.521391519056515	27.943946756047534	23.604157154417493
94-95	21.68943792824645	26.37993775756828	28.074727708884655	23.855896605300618
96-97	21.779076797913053	26.299760581379772	27.991188228572938	23.92997439213424
98-99	21.660967269174403	26.35249262231	27.90574258044307	24.080797528072534
100	21.74631418845111	26.189468097632545	27.914011450181498	24.15020626373485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	122.5
2	400.0
3	712.5
4	462.5
5	329.5
6	753.0
7	2019.5
8	3651.0
9	4278.5
10	3084.0
11	1779.5
12	1710.0
13	1942.5
14	2170.5
15	2471.0
16	2925.5
17	3311.0
18	3768.5
19	4279.0
20	4851.5
21	5767.0
22	7065.0
23	8925.5
24	11221.0
25	13952.0
26	17543.5
27	21647.5
28	25636.0
29	30442.5
30	37012.0
31	43512.0
32	50690.5
33	56557.5
34	60582.5
35	65214.0
36	70365.0
37	76957.0
38	81589.0
39	83773.5
40	85518.0
41	87604.0
42	90724.5
43	94532.0
44	98429.0
45	103692.5
46	108610.5
47	111818.0
48	113693.5
49	112936.5
50	109420.0
51	105842.0
52	102825.5
53	99713.5
54	96717.0
55	89222.0
56	79815.0
57	70312.5
58	61389.0
59	53320.5
60	44149.5
61	36034.0
62	29008.5
63	22075.0
64	15806.0
65	11288.0
66	8296.5
67	6252.5
68	4642.0
69	3349.0
70	2526.5
71	2079.0
72	1621.0
73	1284.5
74	982.5
75	709.5
76	503.5
77	306.0
78	198.5
79	121.0
80	74.5
81	52.5
82	37.0
83	26.5
84	14.5
85	7.5
86	7.5
87	3.5
88	1.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0022907479812783412
2	0.002811372522477965
3	6.941660549328308E-5
4	0.04966758123044404
5	0.0021519147702917752
6	0.003227872155437663
7	3.470830274664154E-5
8	3.1237472471977386E-4
9	3.470830274664154E-5
10-11	1.5618736235988693E-4
12-13	0.0
14-15	0.0
16-17	2.776664219731323E-4
18-19	1.7354151373320768E-4
20-21	2.082498164798492E-4
22-23	0.0
24-25	0.0
26-27	2.6031227059981156E-4
28-29	1.735415137332077E-5
30-31	1.7354151373320768E-4
32-33	2.082498164798492E-4
34-35	8.677075686660384E-5
36-37	1.735415137332077E-5
38-39	7.288743576794722E-4
40-41	5.206245411996231E-4
42-43	0.0
44-45	6.421036008128685E-4
46-47	0.0
48-49	1.2147905961324539E-4
50-51	0.0
52-53	2.9502057334645306E-4
54-55	2.6031227059981156E-4
56-57	1.5618736235988693E-4
58-59	7.115202063061515E-4
60-61	5.900411466929061E-4
62-63	1.7354151373320768E-4
64-65	3.1237472471977386E-4
66-67	9.718324769059631E-4
68-69	0.0023254562840249833
70-71	0.0034881844260374745
72-73	0.002984914036211172
74-75	0.00270724761423804
76-77	0.0043038295405835505
78-79	0.001197436444759133
80-81	0.0010933115365192084
82-83	7.46228509052793E-4
84-85	5.553328439462646E-4
86-87	0.0
88-89	0.0
90-91	3.470830274664154E-5
92-93	5.20624541199623E-5
94-95	5.20624541199623E-5
96-97	3.1237472471977386E-4
98-99	8.677075686660384E-5
100	0.07198501989653455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2881155.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.9748652888929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94278762284404	46.5653708963382
2	15.307813969608974	19.280150452071158
3	5.3305519402135095	10.070723710511773
4	2.320196980205376	5.844563690885186
5	1.1016454870884564	3.468798807275617
6	0.6447689463450665	2.436254252312519
7	0.37794949002338174	1.6660922754159768
8	0.2516339815022192	1.267729286976802
9	0.15808746033376456	0.8959982866543821
>10	0.545543310824435	5.310483552065804
>50	0.01175591072188222	0.5194339088036733
>100	0.005993072450452859	0.7075948948389043
>500	7.481340225989232E-4	0.34864093075487057
>1k	2.992536090394497E-4	0.26006728888734626
>5k	7.481340225986243E-5	0.2966274729773065
>10k+	1.4962680451972485E-4	1.0614702932304187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11835	0.41077276300650256	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10695	0.37120529787533124	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6296	0.2185234740928551	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.470830274664154E-5
2	0.0	0.0	0.0	0.0	3.470830274664154E-5
3	0.0	0.0	0.0	0.0	3.470830274664154E-5
4	0.0	0.0	0.0	0.0	3.470830274664154E-5
5	0.0	0.0	0.0	0.0	3.470830274664154E-5
6	0.0	0.0	0.0	0.0	3.470830274664154E-5
7	0.0	0.0	0.0	0.0	3.470830274664154E-5
8	0.0	0.0	0.0	0.0	3.470830274664154E-5
9	0.0	0.0	0.0	0.0	3.470830274664154E-5
10-11	0.0	0.0	0.0	3.470830274664154E-5	3.470830274664154E-5
12-13	0.0	0.0	0.0	3.470830274664154E-5	2.950205733464531E-4
14-15	0.0	0.0	0.0	6.941660549328308E-5	3.297288760930946E-4
16-17	0.0	0.0	0.0	1.0412490823992461E-4	3.4708302746641536E-4
18-19	0.0	0.0	0.0	1.0412490823992461E-4	3.817913302130569E-4
20-21	0.0	0.0	0.0	1.2147905961324538E-4	5.553328439462646E-4
22-23	0.0	0.0	0.0	2.4295811922649075E-4	6.941660549328307E-4
24-25	0.0	0.0	0.0	5.553328439462646E-4	7.115202063061515E-4
26-27	0.0	0.0	0.0	0.0010586032337725668	7.635826604261138E-4
28-29	0.0	0.0	0.0	0.004182350480970305	7.635826604261138E-4
30-31	0.0	0.0	0.0	0.013779196190416692	7.809368117994346E-4
32-33	0.0	0.0	0.0	0.03495126086586803	8.677075686660384E-4
34-35	0.0	0.0	0.0	0.06908687661718999	9.0241587141268E-4
36-37	0.0	0.0	0.0	0.12215587151680489	9.0241587141268E-4
38-39	0.0	0.0	0.0	0.20125609347640094	9.71832476905963E-4
40-41	0.0	0.0	0.0	0.2891895784850173	9.71832476905963E-4
42-43	0.0	0.0	0.0	0.37661979310380733	0.0010412490823992462
44-45	0.0	0.0	0.0	0.4927884823968165	0.0010759573851458876
46-47	0.0	0.0	0.0	0.6372965008824587	0.0011627281420124916
48-49	0.0	0.0	0.0	0.7915749065912803	0.001266853050252416
50-51	0.0	0.0	0.0	0.9612290904168641	0.0012842072016257368
52-53	0.0	0.0	0.0	1.12321273933544	0.0013883321098656614
54-55	0.0	0.0	0.0	1.3050668915764685	0.001423040412612303
56-57	0.0	0.0	0.0	1.5266273421596548	0.001492457018105586
58-59	0.0	0.0	0.0	1.7748958317063814	0.0015618736235988692
60-61	0.0	0.0	0.0	2.0453949891623324	0.0015618736235988692
62-63	0.0	0.0	0.0	2.318254311205055	0.0016312902290921522
64-65	0.0	0.0	0.0	2.602081456915716	0.0016312902290921522
66-67	0.0	0.0	0.0	2.92370594431747	0.0016659985318387938
68-69	0.0	0.0	0.0	3.287709269372873	0.0017007068345854354
70-71	0.0	0.0	0.0	3.6883992704314763	0.0017354151373320768
72-73	0.0	0.0	0.0	4.101254531602777	0.0018916024996919638
74-75	0.0	0.0	0.0	4.510031567201348	0.00203043571067853
76-77	0.0	0.0	0.0	4.951017907748803	0.002047789862051851
78-79	0.0	0.0	0.0	5.445663284342564	0.002047789862051851
80-81	0.0	0.0	0.0	5.977273003361499	0.002047789862051851
82-83	0.0	0.0	0.0	6.532762034670124	0.0021345606189184544
84-85	0.0	0.0	0.0	7.083704278318939	0.002186623073038417
86-87	0.0	0.0	0.0	7.6391759554761896	0.0022907479812783417
88	0.0	0.0	0.0	8.077767423134125	0.0023601645867716244
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5680	0.0	64.168365	1
GTATCAA	11075	0.0	44.332718	1
ATCAACG	14340	0.0	33.90675	3
CAACGCA	14460	0.0	33.674034	5
TATCAAC	14605	0.0	33.518658	2
TCAACGC	14630	0.0	33.39535	4
AACGCAG	15055	0.0	32.435715	6
ACGCAGA	16810	0.0	28.96397	7
CGCAGAG	17055	0.0	28.382557	8
GCAGAGT	18320	0.0	26.166203	9
TGGTATC	1915	0.0	25.784456	2
GTGGTAT	2070	0.0	25.445751	1
GTACATG	8090	0.0	24.76445	1
TACATGG	7970	0.0	24.19151	2
ACATGGG	7730	0.0	23.602991	3
GAGTACT	13800	0.0	23.0225	12-13
AGAGTAC	16990	0.0	22.599873	10-11
CAGAGTA	17995	0.0	22.526026	10-11
TAACGCC	420	0.0	21.272528	4
CTAACGC	380	0.0	21.036821	3
>>END_MODULE