FastQCFastQC Report
Mon 16 Jan 2017
ERR1146510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.038.1219831
GTATCAA63150.034.849941
ATCAACG73800.028.2185213
CAACGCA74250.028.0462445
TATCAAC77900.027.5781842
AACGCAG77500.027.0456166
TCAACGC78200.026.6904954
GTACATG104300.025.7894021
TACATGG104150.025.4118352
ACATGGG104500.023.6622683
ACGCAGA87600.023.5518027
CGCAGAG89850.022.7521238
CATGGGG74750.021.381994
GCAGAGT98450.019.427199
TCGCCGG3900.019.30791594
ATGGGGG44650.018.8451255
CGTTAAC3055.2223186E-918.488979
GAGTACA83400.017.7612721
GTATAAG12850.017.565791
TACGGGC7100.017.2145394
Produced by FastQC (version 0.11.5)