FastQCFastQC Report
Mon 16 Jan 2017
ERR1146510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159710.4763289476235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145060.43263588468013847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81310.24250395548974257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80200.062.2620661
GTATCAA148350.043.580131
ATCAACG190750.033.595823
TATCAAC193450.033.2737432
CAACGCA192100.033.2509085
TCAACGC196450.032.5971034
AACGCAG199800.032.1802256
ACGCAGA223600.028.6065437
CGCAGAG226150.028.117738
GCAGAGT242100.025.9158559
GTACATG94950.023.620921
GAGTACT177500.023.60489512-13
CAGAGTA235650.022.88570610-11
GTGGTAT26600.022.8024461
AGAGTAC223800.022.16548310-11
TACATGG97900.022.1403832
ACATGGG95950.021.952653
GTACTTT193950.021.31204414-15
AGTACTT184500.021.0153512-13
CATGGGG69450.019.7003844
Produced by FastQC (version 0.11.5)