##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3352935
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27158892134801	33.0	31.0	34.0	30.0	34.0
2	32.39522776313886	34.0	31.0	34.0	30.0	34.0
3	32.48402131267084	34.0	31.0	34.0	30.0	34.0
4	36.004573306670125	37.0	35.0	37.0	35.0	37.0
5	36.0569247539842	37.0	35.0	37.0	35.0	37.0
6	36.13191934827248	37.0	36.0	37.0	35.0	37.0
7	36.09613010690634	37.0	36.0	37.0	35.0	37.0
8	36.09847491824327	37.0	36.0	37.0	35.0	37.0
9	37.87749151116857	39.0	38.0	39.0	35.0	39.0
10-11	37.82824987660065	39.0	38.0	39.0	35.0	39.0
12-13	37.80249617126488	39.0	38.0	39.0	35.0	39.0
14-15	39.13471316920847	40.0	39.0	41.0	36.0	41.0
16-17	39.07816763522108	40.0	38.5	41.0	36.0	41.0
18-19	39.20283542627578	40.0	39.0	41.0	36.0	41.0
20-21	39.2610064018539	40.5	39.0	41.0	36.0	41.0
22-23	39.22304712140259	40.5	39.0	41.0	36.0	41.0
24-25	39.207381145175795	41.0	39.0	41.0	36.0	41.0
26-27	39.11933664088329	40.5	39.0	41.0	35.0	41.0
28-29	39.045333715088425	40.0	39.0	41.0	35.0	41.0
30-31	38.87139207888015	40.0	38.5	41.0	35.0	41.0
32-33	38.725363599354	40.0	38.0	41.0	35.0	41.0
34-35	38.62790734088195	40.0	38.0	41.0	35.0	41.0
36-37	38.53252866518439	40.0	38.0	41.0	35.0	41.0
38-39	38.43373268494618	40.0	38.0	41.0	34.5	41.0
40-41	38.31847127964008	40.0	38.0	41.0	34.0	41.0
42-43	38.191912458786106	40.0	38.0	41.0	34.0	41.0
44-45	38.019326351390646	40.0	38.0	41.0	33.0	41.0
46-47	37.732041778322575	40.0	37.5	41.0	33.0	41.0
48-49	37.40248304843368	40.0	37.0	41.0	32.0	41.0
50-51	37.27212352759597	40.0	37.0	41.0	32.0	41.0
52-53	37.16318538832397	40.0	36.5	41.0	31.0	41.0
54-55	36.99407026977856	40.0	36.0	41.0	31.0	41.0
56-57	36.83979617857191	39.5	35.5	41.0	31.0	41.0
58-59	36.663251300726074	39.0	35.0	41.0	31.0	41.0
60-61	36.459791645230226	39.0	35.0	41.0	31.0	41.0
62-63	36.22118024954256	38.5	35.0	41.0	30.5	41.0
64-65	35.94637727841428	38.0	35.0	40.0	30.0	41.0
66-67	35.67261429165791	37.0	35.0	40.0	30.0	41.0
68-69	35.36948852274202	37.0	35.0	39.5	29.5	41.0
70-71	35.04015541607576	36.0	35.0	39.0	29.0	41.0
72-73	34.70884225313047	36.0	35.0	39.0	29.0	41.0
74-75	34.39331436487734	35.0	34.0	38.5	29.0	40.0
76-77	34.09786753993143	35.0	34.0	37.0	29.0	39.0
78-79	33.81671416236819	35.0	34.0	37.0	29.0	39.0
80-81	33.55933309175394	35.0	34.0	36.0	29.0	39.0
82-83	33.31653282870083	35.0	34.0	36.0	29.0	37.0
84-85	33.111260731269766	35.0	34.0	36.0	28.0	37.0
86-87	32.9271776816431	35.0	34.0	35.0	27.5	37.0
88-89	32.732702393574584	35.0	34.0	35.0	27.0	36.0
90-91	32.65379540611434	35.0	34.0	35.0	27.0	36.0
92-93	32.558157256254596	35.0	34.0	35.0	27.0	36.0
94-95	32.48842670675095	35.0	34.0	35.0	27.0	36.0
96-97	32.433028525754295	35.0	34.0	35.0	27.0	35.0
98-99	32.36045449732846	35.0	34.0	35.0	26.0	35.0
100	31.619620123861633	35.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.306438549275974
1101	2	0.31574888425714676
1101	3	0.2996101476012498
1101	4	0.18905111561941368
1101	5	0.16006283927731602
1101	6	0.20222231178183847
1101	7	0.22312606783158628
1101	8	0.27243563370868884
1101	9	0.28147134954079434
1101	10-11	0.27016747537116004
1101	12-13	0.30477177355266605
1101	14-15	0.4672428494866949
1101	16-17	0.49275185063221016
1101	18-19	0.43892208270018074
1101	20-21	0.3929121987594826
1101	22-23	0.3246811111294363
1101	24-25	0.353484999554631
1101	26-27	0.35099806270562794
1101	28-29	0.38813576812570005
1101	30-31	0.41243087015488555
1101	32-33	0.4707566049308767
1101	34-35	0.37945960242885235
1101	36-37	0.45898667420099315
1101	38-39	0.4846006819271764
1101	40-41	0.5114149437931275
1101	42-43	0.5377395173529962
1101	44-45	0.45294959559763726
1101	46-47	0.5429244205657824
1101	48-49	0.6174160896690779
1101	50-51	0.6975586571374706
1101	52-53	0.6602077400772899
1101	54-55	0.6730706854656603
1101	56-57	0.6366701283653171
1101	58-59	0.5629348631746396
1101	60-61	0.508312983841698
1101	62-63	0.5768046486635683
1101	64-65	0.5523869073514405
1101	66-67	0.5096731788572484
1101	68-69	0.42182799076088173
1101	70-71	0.4247636209003076
1101	72-73	0.4963942615325081
1101	74-75	0.46517470560166885
1101	76-77	0.5232791930048748
1101	78-79	0.45498049649300043
1101	80-81	0.446097258758023
1101	82-83	0.4300744433986097
1101	84-85	0.3770164876687616
1101	86-87	0.3285428542875124
1101	88-89	0.338214817331135
1101	90-91	0.3225851150314938
1101	92-93	0.3658450180154347
1101	94-95	0.3622694678516183
1101	96-97	0.3564318005452236
1101	98-99	0.38635059261313387
1101	100	0.38095945849913093
1102	1	0.30533142156388493
1102	2	0.25547790008652527
1102	3	0.29332770390619345
1102	4	0.2274854200727887
1102	5	0.17853718303294386
1102	6	0.24261970471147976
1102	7	0.22760139673883373
1102	8	0.22770943890092354
1102	9	0.32691761855154766
1102	10-11	0.2857657785019967
1102	12-13	0.2802493099677221
1102	14-15	0.4421343815946983
1102	16-17	0.5226282862519298
1102	18-19	0.4429754781253976
1102	20-21	0.4069971678268658
1102	22-23	0.40967973824955095
1102	24-25	0.41737332758416557
1102	26-27	0.42481929350152825
1102	28-29	0.41367665425349287
1102	30-31	0.46506573549068264
1102	32-33	0.44552107428035725
1102	34-35	0.5045861072116153
1102	36-37	0.47011668151436226
1102	38-39	0.4425259100614056
1102	40-41	0.4569375646493796
1102	42-43	0.4465163462547963
1102	44-45	0.45491587621427954
1102	46-47	0.4673211968673314
1102	48-49	0.5958168221485067
1102	50-51	0.5162026514757017
1102	52-53	0.43238029524811594
1102	54-55	0.4506824661765165
1102	56-57	0.42330860730783115
1102	58-59	0.3823149962272083
1102	60-61	0.32121499239870843
1102	62-63	0.3570568744677871
1102	64-65	0.4153526549065987
1102	66-67	0.34131406358361005
1102	68-69	0.2475513219851493
1102	70-71	0.34602815487117766
1102	72-73	0.3705516792103154
1102	74-75	0.3501028720621804
1102	76-77	0.30719161001570683
1102	78-79	0.348789379155626
1102	80-81	0.28076527875380464
1102	82-83	0.34819593885669065
1102	84-85	0.3365731331081321
1102	86-87	0.24680729534892265
1102	88-89	0.33032727202480316
1102	90-91	0.3364682266365051
1102	92-93	0.3583443705040352
1102	94-95	0.3456328886219211
1102	96-97	0.3115757401239705
1102	98-99	0.28228938552730654
1102	100	0.28410192605997864
1103	1	0.22124971450399045
1103	2	0.20659620448066818
1103	3	0.2528617562194171
1103	4	0.15161684736312964
1103	5	0.16679683834587422
1103	6	0.16179322485731262
1103	7	0.1747998671399813
1103	8	0.19023404530805976
1103	9	0.20918753985390026
1103	10-11	0.24837197984846426
1103	12-13	0.24709791881307552
1103	14-15	0.4015416176769122
1103	16-17	0.46994530500021625
1103	18-19	0.3294653727295369
1103	20-21	0.3592871422765782
1103	22-23	0.34744974578492105
1103	24-25	0.32380124935728105
1103	26-27	0.321204786410334
1103	28-29	0.2848509997761184
1103	30-31	0.31466672691078656
1103	32-33	0.30306856079577216
1103	34-35	0.23959032105961597
1103	36-37	0.30498785805288975
1103	38-39	0.316136155663429
1103	40-41	0.3152639967363484
1103	42-43	0.3407393842191553
1103	44-45	0.3149973178952763
1103	46-47	0.3766504904991592
1103	48-49	0.3715947354949307
1103	50-51	0.351497884753293
1103	52-53	0.3915589545895699
1103	54-55	0.40678403591996926
1103	56-57	0.3349659656312127
1103	58-59	0.33014686719538844
1103	60-61	0.2851157225385563
1103	62-63	0.28417600746522
1103	64-65	0.3699220950231563
1103	66-67	0.39182861584064455
1103	68-69	0.3373388015757044
1103	70-71	0.41778711036319294
1103	72-73	0.350510101837223
1103	74-75	0.3333312175542602
1103	76-77	0.31113355051527236
1103	78-79	0.3073360747269547
1103	80-81	0.2122000202707568
1103	82-83	0.2438098970410607
1103	84-85	0.23134328820506056
1103	86-87	0.22931943540042
1103	88-89	0.2870474710549473
1103	90-91	0.23139471531815303
1103	92-93	0.28249818157754447
1103	94-95	0.3211876043934936
1103	96-97	0.3419108077131696
1103	98-99	0.36791232192776846
1103	100	0.3316080776932715
1104	1	0.18537350568669808
1104	2	0.1823059848682007
1104	3	0.20619401322243647
1104	4	0.15554976390949093
1104	5	0.09287208858800255
1104	6	0.1259423213187958
1104	7	0.14003667039041545
1104	8	0.14735093552648237
1104	9	0.20691935581672993
1104	10-11	0.17416996920866978
1104	12-13	0.24021964523443984
1104	14-15	0.38571911861349406
1104	16-17	0.4182398506014451
1104	18-19	0.332850443997053
1104	20-21	0.24886270271847621
1104	22-23	0.2931470453475029
1104	24-25	0.31171793205373177
1104	26-27	0.2783188986507241
1104	28-29	0.2835693247571598
1104	30-31	0.3155220173201343
1104	32-33	0.3179507493157985
1104	34-35	0.29767317259663173
1104	36-37	0.32708805052241274
1104	38-39	0.2620577944594231
1104	40-41	0.2730686349418363
1104	42-43	0.33913751805288683
1104	44-45	0.31284043973374764
1104	46-47	0.4319694173162958
1104	48-49	0.4638529496257746
1104	50-51	0.32642271126740496
1104	52-53	0.31819229828157347
1104	54-55	0.34514845397139027
1104	56-57	0.24384135328754297
1104	58-59	0.24147978493698474
1104	60-61	0.2791773859936626
1104	62-63	0.22326813903623588
1104	64-65	0.22463589885663282
1104	66-67	0.2659907403187063
1104	68-69	0.2936395031762302
1104	70-71	0.28525787949799053
1104	72-73	0.27040859965610764
1104	74-75	0.24993638741337776
1104	76-77	0.25127718178355707
1104	78-79	0.312299294297965
1104	80-81	0.2673797680546386
1104	82-83	0.2292148674086718
1104	84-85	0.238082242684321
1104	86-87	0.14077068566794537
1104	88-89	0.19954303095898496
1104	90-91	0.2124100368707431
1104	92-93	0.23485884660399847
1104	94-95	0.26584976865034093
1104	96-97	0.2230703786925119
1104	98-99	0.25680914530290266
1104	100	0.22904947122789565
1105	1	0.209330679914423
1105	2	0.19166528638735514
1105	3	0.1687914484309303
1105	4	0.11149958926114323
1105	5	0.1505925583871317
1105	6	0.129012383229977
1105	7	0.1753623406729048
1105	8	0.14548945002376712
1105	9	0.18839611555884517
1105	10-11	0.1942430113254332
1105	12-13	0.15645121501570713
1105	14-15	0.24156960964052132
1105	16-17	0.28270045941587796
1105	18-19	0.2702392226121546
1105	20-21	0.1888624143341744
1105	22-23	0.27515098566179574
1105	24-25	0.268004322987494
1105	26-27	0.2808486796052634
1105	28-29	0.2198621551013744
1105	30-31	0.23965549282927157
1105	32-33	0.3052246489890109
1105	34-35	0.2748385870189409
1105	36-37	0.25785348576466305
1105	38-39	0.2809059945896877
1105	40-41	0.2444390195620869
1105	42-43	0.2795176683503442
1105	44-45	0.33262061352397865
1105	46-47	0.39729700781620636
1105	48-49	0.42090769129674044
1105	50-51	0.24656531730489917
1105	52-53	0.19019424452392286
1105	54-55	0.225487256290279
1105	56-57	0.23367044338583298
1105	58-59	0.17001411040610037
1105	60-61	0.21772902155468188
1105	62-63	0.161317475963358
1105	64-65	0.16529291566872928
1105	66-67	0.15604932453466347
1105	68-69	0.19604454033559904
1105	70-71	0.21990181984042323
1105	72-73	0.26069171918647527
1105	74-75	0.271775783381905
1105	76-77	0.15311186864744997
1105	78-79	0.09110440207631854
1105	80-81	0.1852206629181623
1105	82-83	0.21432351091924318
1105	84-85	0.18950742383889718
1105	86-87	0.17747453398061452
1105	88-89	0.3268590734260357
1105	90-91	0.2600364406340887
1105	92-93	0.1914032008130846
1105	94-95	0.2440410574749592
1105	96-97	0.1806389367850727
1105	98-99	0.219497228138124
1105	100	0.29750539836935985
1106	1	0.19064479671263257
1106	2	0.183448445034756
1106	3	0.2082748323275183
1106	4	0.14589534211414446
1106	5	0.14426549063470162
1106	6	0.14951831052912468
1106	7	0.15267668993054428
1106	8	0.19467210368802768
1106	9	0.1901928887355595
1106	10-11	0.23533298378165313
1106	12-13	0.23210154358112334
1106	14-15	0.3388320251970498
1106	16-17	0.41379612929284804
1106	18-19	0.33512321186906746
1106	20-21	0.36793410525505266
1106	22-23	0.37792604919265926
1106	24-25	0.27181448872473624
1106	26-27	0.28988041947583554
1106	28-29	0.3065310564424948
1106	30-31	0.33999522745585864
1106	32-33	0.2966372005326363
1106	34-35	0.2664323728255624
1106	36-37	0.33513048914947774
1106	38-39	0.3268568210945304
1106	40-41	0.2991877735479136
1106	42-43	0.27045878601062157
1106	44-45	0.2701776147906827
1106	46-47	0.31815753981859984
1106	48-49	0.34678139988552914
1106	50-51	0.3204377100667557
1106	52-53	0.26968720987864714
1106	54-55	0.321950779892326
1106	56-57	0.2625243451324266
1106	58-59	0.24627618526636041
1106	60-61	0.28295907729332725
1106	62-63	0.1990262336059203
1106	64-65	0.24817105064472855
1106	66-67	0.2760525916220544
1106	68-69	0.2869219189858683
1106	70-71	0.22194984818982277
1106	72-73	0.2627327745855865
1106	74-75	0.3418976031129475
1106	76-77	0.28670504116747964
1106	78-79	0.3473684843136695
1106	80-81	0.294977148344735
1106	82-83	0.3453548215058646
1106	84-85	0.33047641664847305
1106	86-87	0.31307912165736695
1106	88-89	0.36098860528171883
1106	90-91	0.28805560738026514
1106	92-93	0.306487041069488
1106	94-95	0.30562090903728745
1106	96-97	0.29128723133048595
1106	98-99	0.24160291675006107
1106	100	0.3850733731864757
1107	1	0.09550479075973328
1107	2	0.10064727293687525
1107	3	0.12643035056104424
1107	4	0.09868656047773072
1107	5	0.11444762713804124
1107	6	0.1312512918462616
1107	7	0.10136248538935888
1107	8	0.10554982677220437
1107	9	0.13934037413716283
1107	10-11	0.11536971123451423
1107	12-13	0.14198496776601388
1107	14-15	0.23662769748959533
1107	16-17	0.3071486179234171
1107	18-19	0.2431962312317566
1107	20-21	0.1985091187672694
1107	22-23	0.15396792293031325
1107	24-25	0.1762983970930705
1107	26-27	0.1911769121724305
1107	28-29	0.20519763571401484
1107	30-31	0.2477863616478828
1107	32-33	0.2086766701873657
1107	34-35	0.17955783464982034
1107	36-37	0.09152887061998882
1107	38-39	0.10565701465328914
1107	40-41	0.11699252920504222
1107	42-43	0.1844458422160855
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2216	50-51	-0.5642830081911612
2216	52-53	-0.5727003513873328
2216	54-55	-0.5536444651336225
2216	56-57	-0.5566925471921635
2216	58-59	-0.5674904416602118
2216	60-61	-0.6094157013307253
2216	62-63	-0.6229518612488789
2216	64-65	-0.552715145030092
2216	66-67	-0.4665778482780141
2216	68-69	-0.43904878134259917
2216	70-71	-0.49247128375320415
2216	72-73	-0.46267881382852494
2216	74-75	-0.4298668992794745
2216	76-77	-0.42381876028054677
2216	78-79	-0.3996888840145729
2216	80-81	-0.4325091430100656
2216	82-83	-0.4700082941175623
2216	84-85	-0.4508967512783144
2216	86-87	-0.3465788435316597
2216	88-89	-0.2917308743677225
2216	90-91	-0.3720994718004036
2216	92-93	-0.38117520356243517
2216	94-95	-0.3449456024658275
2216	96-97	-0.35920936798051883
2216	98-99	-0.3803468959821217
2216	100	-0.4869642643112968
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	19.0
8	124.0
9	397.0
10	491.0
11	297.0
12	291.0
13	356.0
14	621.0
15	1207.0
16	2299.0
17	3906.0
18	6546.0
19	9867.0
20	14104.0
21	20267.0
22	28276.0
23	31168.0
24	28449.0
25	24102.0
26	22000.0
27	24491.0
28	29360.0
29	35056.0
30	42321.0
31	52850.0
32	67685.0
33	87237.0
34	124622.0
35	193977.0
36	347999.0
37	774258.0
38	1045798.0
39	331732.0
40	761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.916214190507354	19.431724740198405	13.613296401535768	24.038764667758475
2	15.679112243427978	21.080967582127354	39.16582235026072	24.074097824183944
3	17.846775576548836	26.138520843740913	31.330972210830843	24.68373136887941
4	12.398786950316348	18.09029121607246	38.18877466524908	31.322147168362118
5	12.628825930685798	37.91516956929824	35.31429873508631	14.141705764929641
6	29.650811641103264	38.42605006300751	17.707988159062268	14.215150136826956
7	25.221701351711665	34.04316935555546	22.976593037620184	17.758536255112688
8	25.486821208347937	34.38830275058344	20.954987063534077	19.16988897753454
9	26.35315815939115	14.689582318053382	20.879355215462038	38.077904307093426
10-11	24.158822667129346	26.0925616053639	28.812119652203165	20.936496075303587
12-13	25.54679705988932	24.43809677193265	28.48022702497961	21.53487914319842
14-15	22.64408982638939	25.310097766598183	27.027623116407433	25.01818929060499
16-17	21.26189596361145	27.982778011901853	28.003058823599584	22.752267200887104
18-19	21.231373019196333	26.36486513892915	30.915449522984662	21.488312318889854
20-21	22.143662303263227	25.590234557851048	30.872442587823677	21.393660551062048
22-23	21.964920286256667	25.530423345516688	30.69279601304529	21.811860355181356
24-25	21.727247918614587	25.761370262173287	30.923981526632637	21.58740029257949
26-27	21.579092410628807	25.889476833070578	30.68126168009673	21.850169076203887
28-29	21.54029552322003	26.036237808999847	30.701151483871815	21.722315183908307
30-31	21.83765298937138	25.827970112101983	30.52821876037339	21.80615813815324
32-33	21.682350721869174	25.959031324118996	30.234026831776113	22.124591122235717
34-35	22.128204921726645	25.82808941078084	30.35674182185188	21.686963845640634
36-37	22.1774156868903	25.64355958744783	30.28288512134225	21.89613960431962
38-39	22.0472995170314	25.71934912051757	30.32215751722497	21.911193845226066
40-41	22.112139021571803	25.61824869885743	30.28255831449527	21.9870539650755
42-43	22.14315219352597	25.748411466372	29.81905405264343	22.2893822874586
44-45	22.093001407431203	25.984418940283938	29.796413093434758	22.126166558850098
46-47	22.38131666733772	26.022126882865308	29.14136420777617	22.455192242020797
48-49	22.564811166820213	26.150298857746336	28.665037446319385	22.619852529114063
50-51	22.495395824851958	26.40081003657989	28.419489193795883	22.684304944772265
52-53	22.449054153644447	26.458182904886275	28.457513041605786	22.635249899863492
54-55	22.269111636721913	26.467327254162747	28.335233912961655	22.928327196153685
56-57	22.43952304402418	26.453967127258842	28.177236029383256	22.929273799333714
58-59	22.20193470717456	26.232179475115725	28.502744108439522	23.063141709270198
60-61	22.06543477213759	26.50398019750894	28.464872991726743	22.96571203862673
62-63	22.107751791866693	26.36213780910297	28.461825007873713	23.068285391156625
64-65	21.791296461133097	26.333675671395422	28.47986242453787	23.395165442933607
66-67	21.910970748289987	26.315071320392093	28.34844662736534	23.425511303952582
68-69	22.053365699861295	26.19062638268707	28.314970577985004	23.441037339466632
70-71	21.942652623730076	26.062641439090317	28.26869978562774	23.726006151551868
72-73	22.05827811764755	26.1295622378246	28.231424347712753	23.5807352968151
74-75	22.03959951020693	26.295195020228345	28.03131788545517	23.63388758410955
76-77	21.9752042513815	26.11517115956326	28.030678904226853	23.87894568482839
78-79	21.924239179646	26.15288464234181	27.935434284531514	23.98744189348068
80-81	21.949836291069648	26.145589701100718	27.931400917772127	23.973173090057507
82-83	21.97709724566575	25.903098363200623	28.08038258047362	24.039421810660006
84-85	21.947108419523246	25.98748760593579	27.788427713135032	24.27697626140593
86-87	21.763797985943658	26.13016655556997	27.74399145822988	24.362044000256493
88-89	21.82634020641617	25.866830105564233	27.632924586966347	24.673905101053258
90-91	21.687274488552415	25.843962332691305	27.71190843601455	24.75685474274173
92-93	21.83451351913586	25.718665675494513	27.745056006943177	24.701764798426453
94-95	21.400231618246842	25.58934257818011	27.88749604450554	25.122929759067514
96-97	21.48558348307821	25.388891788513014	27.88134240998531	25.244182318423462
98-99	21.321363505745197	25.571406863339398	27.66760301175345	25.439626619161952
100	21.501767501608718	25.271064667517486	27.69058991933125	25.536577911542548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	467.0
1	297.0
2	473.0
3	783.0
4	507.5
5	418.5
6	900.0
7	2263.5
8	4172.5
9	5028.0
10	3745.0
11	2280.5
12	2209.5
13	2439.0
14	2701.5
15	3161.5
16	3579.5
17	4051.0
18	4675.0
19	5192.0
20	5807.0
21	6728.5
22	7962.5
23	9481.0
24	11379.5
25	14008.5
26	17824.0
27	22613.5
28	27307.5
29	32894.5
30	39674.5
31	47323.5
32	56631.5
33	63029.5
34	67266.5
35	72005.0
36	77232.0
37	85671.5
38	90625.0
39	91337.0
40	94594.0
41	97756.0
42	99688.5
43	103594.5
44	109793.5
45	119018.0
46	125880.5
47	128962.5
48	133079.5
49	132955.5
50	128463.0
51	126559.0
52	124740.0
53	122226.5
54	119187.5
55	110192.0
56	99989.0
57	88704.5
58	78360.0
59	69255.0
60	56849.0
61	46042.5
62	37600.5
63	28500.0
64	19698.5
65	13780.0
66	10151.0
67	7697.5
68	5756.0
69	4302.0
70	3260.5
71	2501.5
72	1875.0
73	1558.0
74	1277.0
75	980.0
76	743.0
77	506.0
78	338.0
79	229.5
80	147.0
81	81.5
82	46.0
83	28.0
84	22.5
85	18.0
86	13.5
87	9.0
88	5.5
89	2.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002624566238236053
2	0.0036982524266053474
3	2.982461634359151E-4
4	0.05105974318022866
5	0.0017298277479283076
6	0.004771938614974641
7	2.9824616343591508E-5
8	2.982461634359151E-4
9	2.9824616343591508E-5
10-11	1.4912308171795754E-5
12-13	0.0
14-15	1.4912308171795754E-5
16-17	3.5789539612309814E-4
18-19	8.947384903077453E-5
20-21	2.833338552641193E-4
22-23	0.0
24-25	0.0
26-27	2.684215470923236E-4
28-29	1.4912308171795754E-5
30-31	1.7894769806154907E-4
32-33	2.2368462257693633E-4
34-35	1.7894769806154907E-4
36-37	4.473692451538727E-5
38-39	7.605277167615835E-4
40-41	4.921061696692599E-4
42-43	0.0
44-45	5.368430941846472E-4
46-47	0.0
48-49	5.9649232687183016E-5
50-51	0.0
52-53	3.5789539612309814E-4
54-55	3.1315847160771085E-4
56-57	1.4912308171795754E-4
58-59	7.30703100417992E-4
60-61	7.008784840744005E-4
62-63	2.0877231440514057E-4
64-65	3.429830879513024E-4
66-67	0.0011035108047128858
68-69	0.0026394785464078484
70-71	0.0036535155020899603
72-73	0.0029377247098437637
74-75	0.0026991277790950315
76-77	0.00425000782896179
78-79	0.0012228092700872519
80-81	9.842123393385199E-4
82-83	5.815800187000344E-4
84-85	5.815800187000344E-4
86-87	0.0
88-89	0.0
90-91	2.9824616343591508E-5
92-93	7.456154085897877E-5
94-95	1.1929846537436603E-4
96-97	2.982461634359151E-4
98-99	1.342107735461618E-4
100	0.07286153772739405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3352935.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.71813336863084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.07217543450477	39.743629917243396
2	16.075470365219235	18.235413442759548
3	6.229126453690525	10.59913274911457
4	2.9260585092547013	6.638423070893013
5	1.601204603559566	4.540866812757857
6	0.9476801966778868	3.225039107159202
7	0.5674344421943456	2.2528675659233484
8	0.3841052707424566	1.7428587178851769
9	0.2768044642746862	1.4129849269587658
>10	0.896418330675513	7.58139093468318
>50	0.013487739262095785	0.5035125126423855
>100	0.008029974422180683	0.8364634901895979
>500	0.0010791929734101612	0.4161829934814913
>1k	6.937669114763003E-4	0.5832420562068421
>5k	7.708521238625558E-5	0.35549783800733376
>10k+	1.5417042477251116E-4	1.3324938640941473
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15971	0.4763289476235	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14506	0.43263588468013847	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8131	0.24250395548974257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.964923268718302E-5
2	0.0	0.0	0.0	0.0	5.964923268718302E-5
3	0.0	0.0	0.0	0.0	5.964923268718302E-5
4	0.0	0.0	0.0	0.0	5.964923268718302E-5
5	0.0	0.0	0.0	0.0	1.1929846537436605E-4
6	0.0	0.0	0.0	0.0	1.4912308171795754E-4
7	0.0	0.0	0.0	0.0	1.4912308171795754E-4
8	0.0	0.0	0.0	0.0	1.4912308171795754E-4
9	0.0	0.0	0.0	0.0	1.4912308171795754E-4
10-11	0.0	0.0	0.0	0.0	1.4912308171795754E-4
12-13	0.0	0.0	0.0	2.982461634359151E-5	3.429830879513023E-4
14-15	0.0	0.0	0.0	2.982461634359151E-5	4.1754462881028113E-4
16-17	0.0	0.0	0.0	2.982461634359151E-5	4.4736924515387266E-4
18-19	0.0	0.0	0.0	2.982461634359151E-5	4.771938614974642E-4
20-21	0.0	0.0	0.0	5.964923268718302E-5	4.921061696692599E-4
22-23	0.0	0.0	0.0	3.280707797795066E-4	5.070184778410557E-4
24-25	0.0	0.0	0.0	9.245631066513368E-4	5.219307860128515E-4
26-27	0.0	0.0	0.0	0.002028073911364223	5.368430941846472E-4
28-29	0.0	0.0	0.0	0.00797808487191073	5.368430941846472E-4
30-31	0.0	0.0	0.0	0.02475443156518095	5.368430941846472E-4
32-33	0.0	0.0	0.0	0.061095726579847215	5.368430941846472E-4
34-35	0.0	0.0	0.0	0.12098355619777897	5.666677105282386E-4
36-37	0.0	0.0	0.0	0.2150503961454666	5.666677105282386E-4
38-39	0.0	0.0	0.0	0.34923134507528475	5.964923268718302E-4
40-41	0.0	0.0	0.0	0.49315003124128565	5.964923268718302E-4
42-43	1.4912308171795756E-5	0.0	0.0	0.6421538144938688	5.964923268718302E-4
44-45	2.982461634359151E-5	0.0	0.0	0.8336278514197264	6.114046350436259E-4
46-47	2.982461634359151E-5	0.0	0.0	1.0654396819502914	6.561415595590132E-4
48-49	2.982461634359151E-5	0.0	0.0	1.3119997852627625	6.859661759026047E-4
50-51	2.982461634359151E-5	0.0	0.0	1.5791388738523113	7.157907922461963E-4
52-53	2.982461634359151E-5	0.0	0.0	1.8364954882811626	7.157907922461963E-4
54-55	2.982461634359151E-5	0.0	0.0	2.122617945173408	7.157907922461963E-4
56-57	2.982461634359151E-5	0.0	0.0	2.466361560841472	7.157907922461963E-4
58-59	2.982461634359151E-5	0.0	0.0	2.8434938345061864	7.157907922461963E-4
60-61	2.982461634359151E-5	0.0	0.0	3.249466512175154	7.157907922461963E-4
62-63	2.982461634359151E-5	0.0	0.0	3.6526804128323396	7.157907922461963E-4
64-65	2.982461634359151E-5	0.0	0.0	4.07644347415026	7.157907922461963E-4
66-67	2.982461634359151E-5	0.0	0.0	4.558379449646354	7.157907922461963E-4
68-69	2.982461634359151E-5	0.0	0.0	5.097966408534612	7.157907922461963E-4
70-71	2.982461634359151E-5	0.0	0.0	5.67575870095901	7.30703100417992E-4
72-73	2.982461634359151E-5	0.0	0.0	6.263765924481088	7.754400249333792E-4
74-75	2.982461634359151E-5	0.0	0.0	6.85436788962506	8.052646412769707E-4
76-77	2.982461634359151E-5	0.0	0.0	7.5020541704506645	8.052646412769707E-4
78-79	2.982461634359151E-5	0.0	0.0	8.212983550232856	8.201769494487665E-4
80-81	2.982461634359151E-5	0.0	0.0	8.974212145478514	8.350892576205623E-4
82-83	2.982461634359151E-5	0.0	0.0	9.754155687479775	8.947384903077453E-4
84-85	2.982461634359151E-5	0.0	0.0	10.530818521683242	8.947384903077453E-4
86-87	2.982461634359151E-5	0.0	0.0	11.326166478025968	9.543877229949284E-4
88	2.982461634359151E-5	0.0	0.0	11.954690442850815	0.0010140369556821114
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8020	0.0	62.262066	1
GTATCAA	14835	0.0	43.58013	1
ATCAACG	19075	0.0	33.59582	3
TATCAAC	19345	0.0	33.273743	2
CAACGCA	19210	0.0	33.250908	5
TCAACGC	19645	0.0	32.597103	4
AACGCAG	19980	0.0	32.180225	6
ACGCAGA	22360	0.0	28.606543	7
CGCAGAG	22615	0.0	28.11773	8
GCAGAGT	24210	0.0	25.915855	9
GTACATG	9495	0.0	23.62092	1
GAGTACT	17750	0.0	23.604895	12-13
CAGAGTA	23565	0.0	22.885706	10-11
GTGGTAT	2660	0.0	22.802446	1
AGAGTAC	22380	0.0	22.165483	10-11
TACATGG	9790	0.0	22.140383	2
ACATGGG	9595	0.0	21.95265	3
GTACTTT	19395	0.0	21.312044	14-15
AGTACTT	18450	0.0	21.01535	12-13
CATGGGG	6945	0.0	19.700384	4
>>END_MODULE