FastQCFastQC Report
Mon 16 Jan 2017
ERR1146509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857032
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.036.5416031
GTATCAA73850.032.7289921
CAACGCA76800.028.8223085
ATCAACG77250.028.7162553
TATCAAC85800.027.7718582
GTACTAG18600.026.2930151
AACGCAG87450.026.278186
GTACATG122350.025.020561
GGGCACC20000.024.44137694
ACGCAGA90750.024.3896357
CGCAGAG91050.024.3101258
CTAGGCA28150.024.2084124
TAGGCAT27250.024.145195
CTATTGA26350.024.0768369
TACATGG121100.024.0620062
CTGTGCG21950.023.764839
GCTATTG26050.023.4521068
ACATGGG120600.023.0316183
TCAACGC100300.022.4914254
GCATCGT19150.022.086158
Produced by FastQC (version 0.11.5)