FastQCFastQC Report
Mon 16 Jan 2017
ERR1146509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857032
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117900.41266601144124393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101590.35557879645730256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.19079240274522652No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT33550.11742955626678316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78100.055.3206561
GTATCAA137650.040.2435151
ATCAACG167650.032.3080673
CAACGCA170250.031.8146695
TATCAAC176000.031.3113542
AACGCAG187050.029.5742366
GTACTAG17850.029.267151
TCAACGC192050.028.3991764
ACGCAGA196700.027.596227
CGCAGAG197950.027.4219578
GCAGAGT212000.025.3607549
GGGCACC19500.025.30618594
TACATGG119300.024.4178732
GTACATG122850.024.403941
ACATGGG119200.023.745553
CTATTGA27000.023.3245379
GAGTACT142850.023.09557712-13
CATGGGG85150.022.8048614
CAGAGTA210400.022.43758810-11
CTGTGCG23200.022.2831559
Produced by FastQC (version 0.11.5)