##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2857032
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.571684531359814	34.0	31.0	34.0	31.0	34.0
2	32.63869218125663	34.0	31.0	34.0	31.0	34.0
3	32.755769623861404	34.0	31.0	34.0	31.0	34.0
4	36.20255355907809	37.0	37.0	37.0	35.0	37.0
5	36.23091025931806	37.0	37.0	37.0	35.0	37.0
6	36.313216652806126	37.0	37.0	37.0	35.0	37.0
7	36.29399985719446	37.0	37.0	37.0	35.0	37.0
8	36.31110572090197	37.0	37.0	37.0	35.0	37.0
9	38.131593905843545	39.0	39.0	39.0	37.0	39.0
10-11	38.09751640863666	39.0	38.5	39.0	37.0	39.0
12-13	38.06737533916316	39.0	38.0	39.0	35.0	39.0
14-15	39.50295341459248	41.0	39.0	41.0	36.5	41.0
16-17	39.501591336743864	41.0	39.0	41.0	36.5	41.0
18-19	39.56508222519034	41.0	39.5	41.0	37.0	41.0
20-21	39.599280652089305	41.0	40.0	41.0	37.0	41.0
22-23	39.55548187769685	41.0	40.0	41.0	36.5	41.0
24-25	39.525416236149965	41.0	39.0	41.0	36.0	41.0
26-27	39.43361957443949	41.0	39.0	41.0	36.0	41.0
28-29	39.35093761637951	41.0	39.0	41.0	36.0	41.0
30-31	39.22467424236061	41.0	39.0	41.0	35.5	41.0
32-33	39.07220237645221	41.0	39.0	41.0	35.0	41.0
34-35	38.971537770665506	41.0	39.0	41.0	35.0	41.0
36-37	38.871168226327185	41.0	38.5	41.0	35.0	41.0
38-39	38.74854254345068	41.0	38.0	41.0	35.0	41.0
40-41	38.6394244096671	40.0	38.0	41.0	35.0	41.0
42-43	38.51943415404517	40.0	38.0	41.0	35.0	41.0
44-45	38.31772710281159	40.0	38.0	41.0	34.5	41.0
46-47	38.022967016120226	40.0	38.0	41.0	33.5	41.0
48-49	37.734064756712556	40.0	37.0	41.0	33.0	41.0
50-51	37.54025751199147	40.0	37.0	41.0	33.0	41.0
52-53	37.39188623018573	40.0	36.5	41.0	33.0	41.0
54-55	37.244132547342836	40.0	36.0	41.0	33.0	41.0
56-57	37.020111255316706	39.5	35.5	41.0	32.0	41.0
58-59	36.782772646578685	39.0	35.0	41.0	32.0	41.0
60-61	36.595851394034085	39.0	35.0	41.0	32.0	41.0
62-63	36.33808459268219	38.5	35.0	41.0	31.0	41.0
64-65	36.057192569071674	37.5	35.0	41.0	31.0	41.0
66-67	35.81994583889855	37.0	35.0	40.0	31.0	41.0
68-69	35.53112303257366	36.5	35.0	40.0	31.0	41.0
70-71	35.28985657143497	36.0	35.0	39.0	31.0	41.0
72-73	35.000683926536354	35.5	35.0	39.0	30.0	41.0
74-75	34.69300938876428	35.0	35.0	39.0	30.0	41.0
76-77	34.41413764354057	35.0	35.0	37.5	30.0	39.5
78-79	34.146619463835194	35.0	35.0	37.0	30.0	39.0
80-81	33.922162054887735	35.0	35.0	36.5	29.5	39.0
82-83	33.67236821288666	35.0	34.0	36.0	29.0	38.0
84-85	33.468517328472345	35.0	34.0	36.0	29.0	37.0
86-87	33.29112922081377	35.0	34.0	36.0	29.0	37.0
88-89	33.15305271344528	35.0	34.0	35.0	29.0	36.5
90-91	33.06157543912704	35.0	34.0	35.0	29.0	36.0
92-93	32.96898757171778	35.0	34.0	35.0	29.0	36.0
94-95	32.90215790372666	35.0	34.0	35.0	29.0	36.0
96-97	32.85015481100666	35.0	34.0	35.0	29.0	36.0
98-99	32.80302145723254	35.0	34.0	35.0	29.0	35.0
100	32.245259066051766	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2997190441732229
1101	2	0.24445033111055636
1101	3	0.28804701613182715
1101	4	0.15689347301466228
1101	5	0.1462237458368847
1101	6	0.10340036182028456
1101	7	0.12059078431740033
1101	8	0.1580583353723597
1101	9	0.19651693805501935
1101	10-11	0.22769580236351317
1101	12-13	0.23548505400227526
1101	14-15	0.31882737311322984
1101	16-17	0.3163718269957343
1101	18-19	0.3045355434696404
1101	20-21	0.29988412840449286
1101	22-23	0.33739347577465395
1101	24-25	0.29938558684676764
1101	26-27	0.2833804109901479
1101	28-29	0.3326839760348932
1101	30-31	0.34769611671838874
1101	32-33	0.3500204834255314
1101	34-35	0.3630405835207924
1101	36-37	0.4550349611557607
1101	38-39	0.4402182961869414
1101	40-41	0.3809541795432523
1101	42-43	0.4043400001280233
1101	44-45	0.3627477818335336
1101	46-47	0.32763926555000467
1101	48-49	0.322164221081259
1101	50-51	0.24647482552126831
1101	52-53	0.22711973901397897
1101	54-55	0.2531235617136076
1101	56-57	0.26435925308430797
1101	58-59	0.31597658358921876
1101	60-61	0.2697337962847399
1101	62-63	0.21688167108869294
1101	64-65	0.22146620132673434
1101	66-67	0.17860828042429233
1101	68-69	0.24597696660528356
1101	70-71	0.24767889361864803
1101	72-73	0.2504829828013868
1101	74-75	0.1895421057580009
1101	76-77	0.2200014929397156
1101	78-79	0.10541126302808834
1101	80-81	0.16620995773238434
1101	82-83	0.15371611510461491
1101	84-85	0.18923895635238352
1101	86-87	0.15902124701905507
1101	88-89	0.18528242915866144
1101	90-91	0.18750055782316366
1101	92-93	0.12816324509083188
1101	94-95	0.12897104428539308
1101	96-97	0.19728153613623078
1101	98-99	0.13765519399552772
1101	100	0.19194105395703787
1102	1	0.2968355936462572
1102	2	0.2693698653673664
1102	3	0.29670711129413974
1102	4	0.1853453803207259
1102	5	0.23797515459636287
1102	6	0.24079395379157376
1102	7	0.22349144850486624
1102	8	0.2400304362509047
1102	9	0.27352890642085725
1102	10-11	0.23999155253808624
1102	12-13	0.288580427425039
1102	14-15	0.35316050616183503
1102	16-17	0.29011904601520655
1102	18-19	0.3662341744964479
1102	20-21	0.3469391309215837
1102	22-23	0.3647174011388188
1102	24-25	0.3131834724895981
1102	26-27	0.35994774725737955
1102	28-29	0.403320719080412
1102	30-31	0.3607814217736873
1102	32-33	0.3462808968781701
1102	34-35	0.3972831012622251
1102	36-37	0.3908273579144037
1102	38-39	0.32574615471013146
1102	40-41	0.3838986898470935
1102	42-43	0.4047563758405275
1102	44-45	0.4562026934105319
1102	46-47	0.4486867005521944
1102	48-49	0.3984541900641929
1102	50-51	0.35188153884886475
1102	52-53	0.37165570094905576
1102	54-55	0.3354870297790242
1102	56-57	0.40443980002402213
1102	58-59	0.4387979999811691
1102	60-61	0.3883268231283026
1102	62-63	0.3192834639076736
1102	64-65	0.34097415046524304
1102	66-67	0.3284164942634291
1102	68-69	0.3430103708502372
1102	70-71	0.38805661979152717
1102	72-73	0.36819129245239424
1102	74-75	0.29102475406342876
1102	76-77	0.28079429568090575
1102	78-79	0.21429740927924712
1102	80-81	0.17386841272516307
1102	82-83	0.22531130177539183
1102	84-85	0.263718892694321
1102	86-87	0.23018739132149335
1102	88-89	0.2398662970356895
1102	90-91	0.1957890977474932
1102	92-93	0.1863152124823131
1102	94-95	0.19691985800436385
1102	96-97	0.22025852504478394
1102	98-99	0.23184996818802972
1102	100	0.20779775835301706
1103	1	0.1950477223286171
1103	2	0.21230835914278856
1103	3	0.21456610303578572
1103	4	0.13976976094441795
1103	5	0.04063816458034353
1103	6	0.08044081685086013
1103	7	0.09723543963021797
1103	8	0.1307342449529898
1103	9	0.1615740192028099
1103	10-11	0.21679329048952667
1103	12-13	0.19207543543686967
1103	14-15	0.28230662966732467
1103	16-17	0.2469494416764917
1103	18-19	0.2365759099827045
1103	20-21	0.22151388748512346
1103	22-23	0.21243741839507635
1103	24-25	0.2455811675469164
1103	26-27	0.23722896259079107
1103	28-29	0.22205700652032334
1103	30-31	0.3158415105201442
1103	32-33	0.274155604138123
1103	34-35	0.31136975231608943
1103	36-37	0.272188136911069
1103	38-39	0.27968705203541333
1103	40-41	0.27297609647842336
1103	42-43	0.2215143568586413
1103	44-45	0.2700452917095646
1103	46-47	0.2802536301713232
1103	48-49	0.3252405206447335
1103	50-51	0.313681427915796
1103	52-53	0.31435753750766793
1103	54-55	0.2574893716993927
1103	56-57	0.3124343766294686
1103	58-59	0.2963149480891474
1103	60-61	0.25268279502526525
1103	62-63	0.20217511927559428
1103	64-65	0.28527373138474843
1103	66-67	0.2428356363810238
1103	68-69	0.2448158065706849
1103	70-71	0.1721250901477518
1103	72-73	0.15962386979740018
1103	74-75	0.14601520149348346
1103	76-77	0.1526810823196314
1103	78-79	0.13279858023943802
1103	80-81	0.19154683008002849
1103	82-83	0.1797785148901454
1103	84-85	0.14384338425108467
1103	86-87	0.14448635594617087
1103	88-89	0.1881557707199022
1103	90-91	0.17815690250528604
1103	92-93	0.12897760617145337
1103	94-95	0.18043828521994243
1103	96-97	0.2056846929234979
1103	98-99	0.18450435004430688
1103	100	0.20719009164526625
1104	1	0.14069267083705483
1104	2	0.15042643692726898
1104	3	0.14304613023887924
1104	4	0.06296768389626095
1104	5	0.02017448479960393
1104	6	0.05368450440391115
1104	7	0.0620229026861665
1104	8	0.10819645719847415
1104	9	0.12726335170437153
1104	10-11	0.11234134076411806
1104	12-13	0.15161339193237922
1104	14-15	0.174364170344127
1104	16-17	0.22165235527087646
1104	18-19	0.19890704116092195
1104	20-21	0.15044325148500093
1104	22-23	0.18153960128984892
1104	24-25	0.17746861219475818
1104	26-27	0.19581199849683628
1104	28-29	0.19994234957586343
1104	30-31	0.21237580073418627
1104	32-33	0.15349237535602356
1104	34-35	0.13586154792473337
1104	36-37	0.15957206356499398
1104	38-39	0.13009273349333483
1104	40-41	0.1890236200765898
1104	42-43	0.2000570866787541
1104	44-45	0.18384005920493962
1104	46-47	0.2054227546612637
1104	48-49	0.2204910370944262
1104	50-51	0.12212109789933123
1104	52-53	0.0750764252488878
1104	54-55	0.13621925061495688
1104	56-57	0.22556248938934687
1104	58-59	0.16395937503452274
1104	60-61	0.10157619929798756
1104	62-63	0.08509284270400741
1104	64-65	0.01891448437862664
1104	66-67	0.047309893153673954
1104	68-69	0.036839662966997366
1104	70-71	0.10576322254294723
1104	72-73	0.06884697094994863
1104	74-75	0.02942828198804648
1104	76-77	-8.911132562872126E-4
1104	78-79	-0.08485652085175843
1104	80-81	0.019770422115442443
1104	82-83	-0.002898305211502361
1104	84-85	-0.017735699025152485
1104	86-87	0.028611152689983044
1104	88-89	0.002371304094822335
1104	90-91	-0.038639153557632255
1104	92-93	-0.014473645579876404
1104	94-95	-0.06469315378274842
1104	96-97	0.0038423481396421266
1104	98-99	-0.010939579370827346
1104	100	-0.023894299302035904
1105	1	0.21017972996366296
1105	2	0.1985826395297181
1105	3	0.18898375027399794
1105	4	0.13076358967616386
1105	5	0.12713541906300918
1105	6	0.11722920881725685
1105	7	0.12191172188296662
1105	8	0.11805376989727279
1105	9	0.1781736167883281
1105	10-11	0.2033393453525747
1105	12-13	0.14105363808922533
1105	14-15	0.20969554559405879
1105	16-17	0.2281866172819349
1105	18-19	0.19048086616582793
1105	20-21	0.19390553710445602
1105	22-23	0.19077088190424973
1105	24-25	0.2154647670094505
1105	26-27	0.16454506302886784
1105	28-29	0.21894387209982114
1105	30-31	0.17267906383259657
1105	32-33	0.20052409189734277
1105	34-35	0.2013725435764897
1105	36-37	0.20226068478725523
1105	38-39	0.25514694874944155
1105	40-41	0.2170229168376281
1105	42-43	0.11223732908731421
1105	44-45	0.12894248868666125
1105	46-47	0.18974580592924184
1105	48-49	0.0929261659113294
1105	50-51	0.13080964572247922
1105	52-53	0.17237884817401294
1105	54-55	0.13204220991644888
1105	56-57	0.16009783962208246
1105	58-59	0.14932887140633966
1105	60-61	0.08251610979168333
1105	62-63	0.10130668566122836
1105	64-65	0.12762318069428602
1105	66-67	0.06907885971732952
1105	68-69	0.12879914520441105
1105	70-71	0.15174037075810531
1105	72-73	0.19369074397783947
1105	74-75	0.13738753851593088
1105	76-77	0.08955538255968065
1105	78-79	0.09824638615940273
1105	80-81	0.1259571117514895
1105	82-83	0.19044018830210518
1105	84-85	0.1307404860079231
1105	86-87	0.08448129775912605
1105	88-89	0.12046424420231006
1105	90-91	0.11392072764605388
1105	92-93	0.07016260667867158
1105	94-95	0.09632978329592845
1105	96-97	0.06264671715673842
1105	98-99	0.09607919658722608
1105	100	0.008234060375677643
1106	1	0.08070226669831726
1106	2	0.04895896550624457
1106	3	0.10036019304309463
1106	4	0.05044571076467008
1106	5	0.013164082329709004
1106	6	0.02247299215180476
1106	7	0.04132775902638741
1106	8	0.011188905672874228
1106	9	0.09415818662774456
1106	10-11	0.11260397531478361
1106	12-13	0.051987352380741925
1106	14-15	0.10324910345124039
1106	16-17	0.10599663808455517
1106	18-19	0.09259498097123497
1106	20-21	0.0777949328467713
1106	22-23	0.08964341042956647
1106	24-25	0.09713060145851671
1106	26-27	0.09677165415838829
1106	28-29	0.13208083593305275
1106	30-31	0.1595793720634262
1106	32-33	0.0800570570237582
1106	34-35	0.08410748706750582
1106	36-37	0.0860105387965433
1106	38-39	0.10626624474413404
1106	40-41	0.08326749402313993
1106	42-43	0.05845878472899102
1106	44-45	0.06365923284829478
1106	46-47	0.05757612155693437
1106	48-49	0.1043064002073848
1106	50-51	0.07468983347076374
1106	52-53	0.1395193090138065
1106	54-55	0.04582369947874554
1106	56-57	0.09153907687857554
1106	58-59	0.13163550776336308
1106	60-61	0.0771530287697999
1106	62-63	0.011065466685316494
1106	64-65	0.04593368305467749
1106	66-67	-0.018883922383281515
1106	68-69	-0.011029186642218747
1106	70-71	-0.017413424746564488
1106	72-73	0.017331659293070345
1106	74-75	0.05239920930982578
1106	76-77	0.008782418855084018
1106	78-79	0.023851549406636252
1106	80-81	0.09607233016206607
1106	82-83	0.12747690687093893
1106	84-85	0.06613831705139717
1106	86-87	0.060850673731344784
1106	88-89	0.09874949228496632
1106	90-91	0.16456645515374646
1106	92-93	-5.628106008757072E-4
1106	94-95	0.03661431708199814
1106	96-97	0.05783526945357664
1106	98-99	0.1286930312597221
1106	100	0.14048489019536703
1107	1	0.08067434679472996
1107	2	0.1494290247485921
1107	3	0.11190656774855512
1107	4	0.10720604796587452
1107	5	0.14323470887396894
1107	6	0.14996080501848752
1107	7	0.128667068533467
1107	8	0.0743276370662258
1107	9	0.11248693554259148
1107	10-11	0.11754191871814612
1107	12-13	0.08042514155685154
1107	14-15	0.17145205380278128
1107	16-17	0.13628858869367377
1107	18-19	0.11632880698380177
1107	20-21	0.09608946556998887
1107	22-23	0.12552175851252656
1107	24-25	0.11681464227626748
1107	26-27	0.17227947522933107
1107	28-29	0.17497080034948453
1107	30-31	0.1606035682532152
1107	32-33	0.14704189823328306
1107	34-35	0.15328267333268997
1107	36-37	0.1323224085714969
1107	38-39	0.1008071722064443
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2216	24-25	-0.4365978682474534
2216	26-27	-0.47967082551578244
2216	28-29	-0.4170704715593132
2216	30-31	-0.3995715146999288
2216	32-33	-0.4774163113429637
2216	34-35	-0.5536069077138137
2216	36-37	-0.4841315957772281
2216	38-39	-0.4162773186663955
2216	40-41	-0.4400455109158159
2216	42-43	-0.47850295663760534
2216	44-45	-0.4014380735624812
2216	46-47	-0.3984174300013521
2216	48-49	-0.44363234575336463
2216	50-51	-0.3686568297890105
2216	52-53	-0.40538152721076415
2216	54-55	-0.4184695630079176
2216	56-57	-0.37656391758319785
2216	58-59	-0.3864721352408935
2216	60-61	-0.3782394960044684
2216	62-63	-0.4438443895587696
2216	64-65	-0.4024522260124428
2216	66-67	-0.28945559049171266
2216	68-69	-0.2807755075722582
2216	70-71	-0.33646551202423325
2216	72-73	-0.3286414612540014
2216	74-75	-0.3830817301267402
2216	76-77	-0.34441582968082685
2216	78-79	-0.35752472701848603
2216	80-81	-0.4140233805569835
2216	82-83	-0.30724548894475845
2216	84-85	-0.40218353858929845
2216	86-87	-0.36428969070207273
2216	88-89	-0.3518063112612708
2216	90-91	-0.3522909864649719
2216	92-93	-0.3463033808836542
2216	94-95	-0.37102741472071443
2216	96-97	-0.3631745622650797
2216	98-99	-0.34326275319978095
2216	100	-0.46765980774686255
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	17.0
8	84.0
9	155.0
10	239.0
11	234.0
12	178.0
13	160.0
14	367.0
15	662.0
16	1380.0
17	2626.0
18	4783.0
19	7362.0
20	10526.0
21	15630.0
22	22974.0
23	26418.0
24	24654.0
25	19741.0
26	15836.0
27	16876.0
28	19614.0
29	23311.0
30	27747.0
31	35055.0
32	45741.0
33	60902.0
34	92364.0
35	153860.0
36	272668.0
37	680884.0
38	879862.0
39	393004.0
40	1118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.126202679945635	21.16352666871788	13.645943485287463	23.06432716604903
2	15.221740358223723	21.617171082274016	40.217308283879795	22.94378027562247
3	17.14355517116219	28.30274267621552	31.717586148118304	22.83611600450398
4	11.820553637481135	18.743262238574857	40.42831861876241	29.007865505181602
5	11.826416288844909	39.21453692848769	35.69106015594689	13.267986626720507
6	27.185740083385635	40.20719345488225	18.802547003742237	13.804519457989874
7	23.69584939895668	35.074650896454784	24.225909965306656	17.003589739281885
8	25.350818611762136	34.135704465333255	21.591357744680494	18.922119178224115
9	26.534319531597827	14.301554900330132	22.11739315485441	37.04673241321763
10-11	24.015586804768024	26.61233755869728	28.957358545511564	20.41471709102313
12-13	25.344220855769205	25.103184003539337	28.660652033298895	20.891943107392567
14-15	22.510094830946038	25.592717476163635	27.549608746000875	24.347578946889456
16-17	20.692557871245405	28.202169244166676	28.501815870455772	22.603457014132147
18-19	20.2963249974099	27.119279728053446	31.795391161177054	20.7890041133596
20-21	21.901837291286903	26.246205852787085	31.051472297125127	20.800484558800882
22-23	21.982887387205285	26.109973852084455	30.793987641999145	21.113151118711116
24-25	21.5342355283385	26.343492127494546	31.300611963744196	20.82166038042276
26-27	21.35691864844356	26.661776976946705	30.69755606517533	21.283748309434404
28-29	20.81533606309068	26.97944596671264	31.080434738096073	21.1247832321006
30-31	21.40360167915721	26.445028462264204	30.99888573040136	21.15248412817723
32-33	21.589437816439357	27.000439274269905	30.15532108148975	21.254801827800986
34-35	21.732626179452694	26.827861816228076	30.69495282189225	20.744559182426975
36-37	21.72541205307867	26.451078393471207	30.54711674412833	21.27639280932179
38-39	21.695853950757595	26.327305934283018	30.87739660603099	21.099443508928392
40-41	21.46208703617398	26.01678448027982	30.980007066891684	21.54112141665452
42-43	21.628574163694303	26.258424659727837	30.253854154397885	21.85914702217997
44-45	21.55917600027021	27.07577190253255	30.016830500219548	21.348221596977684
46-47	21.95528565553329	26.59311696607485	29.2466639248869	22.204933453504957
48-49	22.442425008216624	26.58012056647975	29.132349065265018	21.845105360038612
50-51	22.17864130682077	26.940490232795828	28.48612710491038	22.39474135547303
52-53	21.633526461120272	27.366947112061133	28.55837043223266	22.441155994585934
54-55	21.680829336546516	27.388512180246117	28.277439571295936	22.65321891191143
56-57	22.46325780074981	26.91348375446841	28.103420334819624	22.51983810996216
58-59	21.656532627127024	27.055321652717378	28.912280922821516	22.375864797334085
60-61	21.861188927638185	27.59397676454019	28.23948593080885	22.305348377012777
62-63	22.19146345667894	27.063987935594668	28.001334966981968	22.743213640744425
64-65	21.427932624161265	27.276738296184377	28.25216637827127	23.04316270138309
66-67	22.010468315991442	26.947889264139373	28.208466045353603	22.83317637451558
68-69	21.634779796305324	26.74982839929533	28.48541945302691	23.129972351372437
70-71	21.731082262870796	26.632444067278698	28.376050037425866	23.26042363242464
72-73	22.104850672866434	26.609744469670083	28.560805101505764	22.72459975595772
74-75	22.10443451692359	27.07970691606267	28.129032958116916	22.686825608896825
76-77	21.949325523994972	26.917190409326963	27.88601296885476	23.247471097823304
78-79	21.755817103639263	27.047580579109738	27.969314730175576	23.22728758707542
80-81	21.613867400481933	27.067849969452563	28.315753606496813	23.002529023568695
82-83	22.14136120798235	26.399386635450085	28.950866258421993	22.508385898145573
84-85	22.383055547728855	26.879666450766432	27.783839823845014	22.9534381776597
86-87	21.398132747550605	27.258830142609536	28.23431799153807	23.108719118301792
88-89	21.917373578417852	26.50933067509314	28.077971234443627	23.495324512045386
90-91	21.81159392390212	26.436219978012137	28.362432889247614	23.389753208838126
92-93	22.203566498380138	26.522699080724333	28.182183468718584	23.091550952176945
94-95	21.03303358170297	26.715626566310775	28.63770864309535	23.613631208890908
96-97	21.472495817320144	26.560349595032584	28.316357603376947	23.650796984270322
98-99	21.149864053963995	26.93828105967905	28.059695850495764	23.85215903586119
100	21.599323770922148	26.369608893182406	27.78111458792728	24.249952747968162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	422.0
1	270.5
2	295.5
3	432.5
4	271.0
5	226.5
6	462.5
7	1090.5
8	2154.5
9	2672.5
10	1956.5
11	1296.0
12	1403.5
13	1676.0
14	1949.5
15	2311.0
16	2771.5
17	3363.5
18	4019.0
19	4651.5
20	5457.0
21	6411.0
22	7522.5
23	8893.5
24	10507.0
25	12308.5
26	15624.5
27	19752.5
28	23544.0
29	30432.0
30	38855.0
31	46042.0
32	55595.0
33	63326.0
34	68238.5
35	73321.0
36	78346.5
37	88996.0
38	94148.0
39	91449.5
40	89626.5
41	86189.5
42	84616.5
43	87161.0
44	92961.5
45	105846.0
46	115772.0
47	120017.5
48	121039.0
49	114284.0
50	106581.0
51	104698.0
52	103721.0
53	99746.5
54	95504.0
55	84502.5
56	74843.5
57	62902.5
58	50645.5
59	47023.0
60	38336.5
61	27338.5
62	20462.0
63	15843.0
64	10675.5
65	5912.0
66	4005.5
67	2874.5
68	1987.5
69	1538.0
70	1293.0
71	1117.0
72	1008.0
73	953.5
74	835.0
75	692.5
76	545.0
77	407.5
78	335.0
79	272.5
80	209.0
81	159.0
82	105.0
83	70.0
84	44.0
85	26.0
86	19.5
87	12.5
88	9.5
89	4.0
90	2.5
91	2.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006405248523642717
2	0.09933385415354117
3	1.7500679026346224E-4
4	0.0
5	0.04158161336659862
6	0.006475251239748102
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.750067902634622E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.775224078694253E-4
24-25	0.0
26-27	0.0
28-29	5.250203707903867E-5
30-31	1.2250475318442355E-4
32-33	0.0016450638284765447
34-35	0.0013825536430813515
36-37	9.625373464490423E-4
38-39	8.050312352119263E-4
40-41	0.001662564507502891
42-43	6.125237659221177E-4
44-45	5.775224078694253E-4
46-47	2.450095063688471E-4
48-49	6.650258030011564E-4
50-51	7.700298771592338E-4
52-53	0.0010150393835280807
54-55	0.001732567223608276
56-57	0.0010850420996334658
58-59	0.002677603891030972
60-61	0.0021875848782932777
62-63	0.0011375441367125045
64-65	0.004637679941981749
66-67	0.004392670435612902
68-69	0.0038151480277434768
70-71	0.0017675685816609685
72-73	0.005250203707903867
74-75	0.0036051398794273216
76-77	0.0038676500648225157
78-79	9.100353093700035E-4
80-81	1.750067902634622E-5
82-83	1.2250475318442355E-4
84-85	8.750339513173112E-5
86-87	0.0
88-89	1.4000543221076976E-4
90-91	3.500135805269244E-5
92-93	0.0
94-95	0.0
96-97	4.2001629663230937E-4
98-99	5.60021728843079E-4
100	4.2001629663230937E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2857032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.762686297223794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.39531462251215	31.712185839205237
2	16.300160264307216	15.57078882597156
3	6.68604876492032	9.580309491804895
4	3.483458645976328	6.655173701484774
5	2.041473462042578	4.875312827582354
6	1.2646156438424339	3.624086417004472
7	0.863791519667834	2.887990236606788
8	0.6247311593438464	2.3871070707072852
9	0.46431295878657414	1.9959150774833048
>10	1.8049769779649383	13.746979316787597
>50	0.04455312716765306	1.4347124876622208
>100	0.0219832193910643	2.0927895472119737
>500	0.003271167197936982	1.0243701834797392
>1k	9.159268154223549E-4	0.6998915066066499
>5k	1.3084668791747927E-4	0.3406651131808955
>10k+	2.6169337583495855E-4	1.3717223572202302
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11790	0.41266601144124393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10159	0.35557879645730256	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5451	0.19079240274522652	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	3355	0.11742955626678316	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.500135805269245E-5	0.0
16-17	0.0	0.0	0.0	1.0500407415807733E-4	0.0
18-19	0.0	0.0	0.0	1.57506111237116E-4	0.0
20-21	0.0	0.0	0.0	2.9751154344788574E-4	0.0
22-23	0.0	0.0	0.0	5.775224078694253E-4	0.0
24-25	0.0	0.0	0.0	0.0016975658655555836	0.0
26-27	0.0	0.0	0.0	0.0038326487067698225	0.0
28-29	0.0	0.0	0.0	0.010342901304570617	0.0
30-31	0.0	0.0	0.0	0.026653534157125295	0.0
32-33	0.0	0.0	0.0	0.06074485690044774	0.0
34-35	0.0	0.0	0.0	0.11569698904317487	0.0
36-37	0.0	0.0	0.0	0.19796768114602847	0.0
38-39	0.0	0.0	0.0	0.30897448821014256	0.0
40-41	0.0	0.0	0.0	0.4240589534873953	0.0
42-43	0.0	0.0	0.0	0.5377958664796194	0.0
44-45	0.0	0.0	0.0	0.6778538007274681	0.0
46-47	0.0	0.0	0.0	0.8361999445578489	0.0
48-49	0.0	0.0	0.0	1.0081616166707268	0.0
50-51	0.0	0.0	0.0	1.1777956984731008	0.0
52-53	0.0	0.0	0.0	1.3408495249615684	0.0
54-55	0.0	0.0	0.0	1.515051283989819	0.0
56-57	0.0	0.0	0.0	1.7160815839654577	0.0
58-59	0.0	0.0	0.0	1.9411403162442702	0.0
60-61	0.0	0.0	0.0	2.1664265573504253	0.0
62-63	0.0	0.0	0.0	2.384170005796225	0.0
64-65	1.7500679026346224E-5	0.0	0.0	2.6077236796787715	0.0
66-67	3.500135805269245E-5	0.0	0.0	2.8513681330835636	0.0
68-69	3.500135805269245E-5	0.0	0.0	3.1235386933013	0.0
70-71	3.500135805269245E-5	0.0	0.0	3.410094811678693	0.0
72-73	3.500135805269245E-5	0.0	0.0	3.704176222037415	0.0
74-75	3.500135805269245E-5	0.0	0.0	3.988072237202803	0.0
76-77	3.500135805269245E-5	0.0	0.0	4.302454435232088	0.0
78-79	3.500135805269245E-5	0.0	0.0	4.634652324510191	0.0
80-81	3.500135805269245E-5	0.0	0.0	4.9926812160311815	0.0
82-83	3.500135805269245E-5	0.0	0.0	5.365305673860146	0.0
84-85	3.500135805269245E-5	0.0	0.0	5.727307219520117	0.0
86-87	3.500135805269245E-5	0.0	0.0	6.105391889205301	0.0
88	3.500135805269245E-5	0.0	0.0	6.396463182771492	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7810	0.0	55.320656	1
GTATCAA	13765	0.0	40.243515	1
ATCAACG	16765	0.0	32.308067	3
CAACGCA	17025	0.0	31.814669	5
TATCAAC	17600	0.0	31.311354	2
AACGCAG	18705	0.0	29.574236	6
GTACTAG	1785	0.0	29.26715	1
TCAACGC	19205	0.0	28.399176	4
ACGCAGA	19670	0.0	27.59622	7
CGCAGAG	19795	0.0	27.421957	8
GCAGAGT	21200	0.0	25.360754	9
GGGCACC	1950	0.0	25.306185	94
TACATGG	11930	0.0	24.417873	2
GTACATG	12285	0.0	24.40394	1
ACATGGG	11920	0.0	23.74555	3
CTATTGA	2700	0.0	23.324537	9
GAGTACT	14285	0.0	23.095577	12-13
CATGGGG	8515	0.0	22.804861	4
CAGAGTA	21040	0.0	22.437588	10-11
CTGTGCG	2320	0.0	22.283155	9
>>END_MODULE