FastQCFastQC Report
Mon 16 Jan 2017
ERR1146508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888630
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.030.6356121
GGTATCA57750.026.9494191
GTACATG108700.026.0832181
GCATCGT19100.025.5873438
TACATGG110400.025.3268182
GTATCAA80800.024.9642961
ACATGGG112850.023.90293
TAGGCAT25550.023.5428645
CTAGGCA29000.023.0107424
GCTATTG20850.022.9889628
CATGGGG92100.022.5529254
CAACGCA82300.022.097925
TAGTACT25100.022.0926864
ATCAACG83600.021.9791433
CTATTGA22500.021.5119579
GGCTATT23850.021.2787237
CTGTGCG28200.020.9964899
TATCAAC95050.020.8637832
GGGCTAT24300.020.6923716
AACGCAG92600.020.6038236
Produced by FastQC (version 0.11.5)