##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146508_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2888630
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47933137854277	31.0	31.0	34.0	28.0	34.0
2	31.533620089800355	33.0	31.0	34.0	28.0	34.0
3	31.710109636748218	33.0	31.0	34.0	29.0	34.0
4	35.16221876806652	37.0	35.0	37.0	32.0	37.0
5	35.22594067083704	37.0	35.0	37.0	32.0	37.0
6	35.184653624728675	37.0	35.0	37.0	32.0	37.0
7	35.15698549139211	37.0	35.0	37.0	32.0	37.0
8	35.14586222534558	37.0	35.0	37.0	32.0	37.0
9	36.72328335577765	39.0	37.0	39.0	32.0	39.0
10-11	36.687306439384756	39.0	37.0	39.0	32.0	39.0
12-13	36.62871482328993	39.0	37.0	39.0	32.0	39.0
14-15	38.023989226726854	40.0	38.0	41.0	33.0	41.0
16-17	38.05566462302198	40.0	38.0	41.0	33.0	41.0
18-19	38.08744422096288	40.0	38.0	41.0	33.0	41.0
20-21	38.1828354617933	40.0	38.5	41.0	33.0	41.0
22-23	38.21990788020619	40.0	38.0	41.0	33.0	41.0
24-25	38.27562304621914	40.0	38.0	41.0	33.0	41.0
26-27	38.30392867899316	40.0	38.0	41.0	33.0	41.0
28-29	38.27070895199454	40.0	38.0	41.0	33.0	41.0
30-31	38.25350062140184	40.0	38.0	41.0	33.0	41.0
32-33	38.25889227765411	40.0	38.0	41.0	33.0	41.0
34-35	38.197264274067635	40.0	38.0	41.0	33.0	41.0
36-37	38.097147609766566	40.0	38.0	41.0	33.0	41.0
38-39	38.03763029533031	40.0	38.0	41.0	32.0	41.0
40-41	37.95660624586742	40.0	38.0	41.0	32.0	41.0
42-43	37.81081516151255	40.0	37.5	41.0	32.0	41.0
44-45	37.641678927380795	40.0	37.0	41.0	32.0	41.0
46-47	37.51249381194546	40.0	37.0	41.0	31.0	41.0
48-49	37.36556724121816	40.0	36.0	41.0	31.0	41.0
50-51	37.20168817051682	40.0	36.0	41.0	31.0	41.0
52-53	37.03430415110277	39.5	35.0	41.0	30.5	41.0
54-55	36.82147367437159	39.0	35.0	41.0	30.0	41.0
56-57	36.64418149780346	39.0	35.0	41.0	30.0	41.0
58-59	36.37395080020633	38.5	35.0	40.5	30.0	41.0
60-61	36.15203781723516	37.5	35.0	40.0	30.0	41.0
62-63	35.881539691826234	37.0	35.0	40.0	30.0	41.0
64-65	35.597419364889234	36.5	35.0	40.0	30.0	41.0
66-67	35.324565278349944	36.0	35.0	39.5	29.5	41.0
68-69	35.03168647421096	35.5	34.0	39.0	29.0	41.0
70-71	34.79497599207929	35.0	34.0	39.0	29.0	41.0
72-73	34.507625067938775	35.0	34.0	37.5	29.0	40.0
74-75	34.220428715342564	35.0	34.0	37.0	29.0	39.5
76-77	33.96237333961082	35.0	34.0	36.5	28.5	39.0
78-79	33.72102207620914	35.0	34.0	36.0	28.0	39.0
80-81	33.502679990168346	35.0	34.0	36.0	28.0	38.0
82-83	33.2869815448846	35.0	34.0	35.5	28.0	37.0
84-85	33.11230133315793	35.0	33.5	35.0	28.0	37.0
86-87	32.96178984501303	35.0	33.0	35.0	27.0	36.5
88-89	32.857387585118204	35.0	33.0	35.0	27.0	36.0
90-91	32.71695665419247	35.0	33.0	35.0	27.0	36.0
92-93	32.64198028130983	35.0	33.0	35.0	27.0	36.0
94-95	32.582000636980155	35.0	33.0	35.0	27.0	35.5
96-97	32.521321699213814	35.0	33.0	35.0	27.0	35.0
98-99	32.46531487244819	35.0	33.0	35.0	27.0	35.0
100	31.523197155745112	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33803770393712185
1101	2	0.36832836614087583
1101	3	0.4041732909371518
1101	4	0.4441246142191986
1101	5	0.3230656900332036
1101	6	0.3516441491754989
1101	7	0.3772864074326918
1101	8	0.4077765612310742
1101	9	0.48812115010782264
1101	10-11	0.40914900796969533
1101	12-13	0.4093789904487224
1101	14-15	0.4665818317355388
1101	16-17	0.43128182718898955
1101	18-19	0.30072276964838096
1101	20-21	0.4218221944234699
1101	22-23	0.3458260141100027
1101	24-25	0.3846745841334993
1101	26-27	0.38332770576890596
1101	28-29	0.5112080600183333
1101	30-31	0.4160421478327265
1101	32-33	0.3966995982207209
1101	34-35	0.41753071295450184
1101	36-37	0.41837558034342237
1101	38-39	0.45798284585807636
1101	40-41	0.4196283511784671
1101	42-43	0.44407052447223094
1101	44-45	0.3870989567814007
1101	46-47	0.407347698749966
1101	48-49	0.3935240713180832
1101	50-51	0.4141743664591644
1101	52-53	0.4144702564943188
1101	54-55	0.3945473325121611
1101	56-57	0.3193268189340017
1101	58-59	0.3458399520244839
1101	60-61	0.3072333266183165
1101	62-63	0.409552261907713
1101	64-65	0.3628837000950398
1101	66-67	0.28571564331729604
1101	68-69	0.29631824550183694
1101	70-71	0.3528452398333073
1101	72-73	0.2925352152526344
1101	74-75	0.33964334013733577
1101	76-77	0.34384843818915556
1101	78-79	0.39275321437749966
1101	80-81	0.30181279781222514
1101	82-83	0.25879162345491835
1101	84-85	0.35571648746335427
1101	86-87	0.33237981121568083
1101	88-89	0.26717802608057895
1101	90-91	0.36282234267132196
1101	92-93	0.3346226830861809
1101	94-95	0.22953655418734797
1101	96-97	0.2602838901404354
1101	98-99	0.2160299298514161
1101	100	0.15079105901278567
1102	1	0.15359954618064364
1102	2	0.1679599341514546
1102	3	0.25783792345971435
1102	4	0.13013501949351536
1102	5	0.17509274064729397
1102	6	0.15982742741930878
1102	7	0.1750217546612518
1102	8	0.23518242725823058
1102	9	0.2921336479978365
1102	10-11	0.30752660836331813
1102	12-13	0.26227828347054327
1102	14-15	0.3143653679740055
1102	16-17	0.32263413068111646
1102	18-19	0.22245203908104827
1102	20-21	0.205224891319169
1102	22-23	0.3109234337367326
1102	24-25	0.2843992656207277
1102	26-27	0.28298200370538495
1102	28-29	0.33418798899754165
1102	30-31	0.2599214210218008
1102	32-33	0.27077946496467575
1102	34-35	0.323871622320425
1102	36-37	0.3005336679792521
1102	38-39	0.2945797937800805
1102	40-41	0.2952449036833329
1102	42-43	0.41111800807964727
1102	44-45	0.2619108211585228
1102	46-47	0.16108039917397576
1102	48-49	0.25812753509414677
1102	50-51	0.2734971052036954
1102	52-53	0.25557214340898327
1102	54-55	0.3091766558228528
1102	56-57	0.28762518842962237
1102	58-59	0.27478616880634377
1102	60-61	0.29131650230323203
1102	62-63	0.3077777781168649
1102	64-65	0.271841771956602
1102	66-67	0.24606715713819227
1102	68-69	0.3022086130885242
1102	70-71	0.2474139453653308
1102	72-73	0.2298517642492115
1102	74-75	0.13527875909009168
1102	76-77	0.06984153180867736
1102	78-79	0.116728254463915
1102	80-81	0.1948645354240952
1102	82-83	0.08959283201492241
1102	84-85	0.1593341932660337
1102	86-87	0.15577533830403922
1102	88-89	0.16607464531097094
1102	90-91	0.20516688882538858
1102	92-93	0.22238268327421906
1102	94-95	0.15936397998885354
1102	96-97	0.1680079646838024
1102	98-99	0.16477982310085793
1102	100	0.1282659723043409
1103	1	0.05745095832826408
1103	2	0.07115712225793303
1103	3	0.1737618883443588
1103	4	0.15991054165236562
1103	5	0.11204958791739728
1103	6	0.15927372002879991
1103	7	0.10026894811102949
1103	8	0.0916580908495277
1103	9	0.09551178256712234
1103	10-11	0.17509497287907294
1103	12-13	0.21847668932098685
1103	14-15	0.24907906126085066
1103	16-17	0.2443244859641922
1103	18-19	0.11822093126387756
1103	20-21	0.2346377207070347
1103	22-23	0.2055645851710466
1103	24-25	0.2106174053881702
1103	26-27	0.19803273534127186
1103	28-29	0.22976050677225146
1103	30-31	0.2185480172492973
1103	32-33	0.2773414953647375
1103	34-35	0.29238397754016177
1103	36-37	0.3700798522055777
1103	38-39	0.33921310343139055
1103	40-41	0.29291430268776253
1103	42-43	0.3110987458240402
1103	44-45	0.29898922018801954
1103	46-47	0.24193260391064797
1103	48-49	0.2740301102994067
1103	50-51	0.2106895357969094
1103	52-53	0.2869518502928585
1103	54-55	0.25935663752370886
1103	56-57	0.2199864705042458
1103	58-59	0.2775484389291307
1103	60-61	0.2242822448433941
1103	62-63	0.16771561776253918
1103	64-65	0.21476167263725188
1103	66-67	0.19076815679648718
1103	68-69	0.19516526542724222
1103	70-71	0.14225979949464573
1103	72-73	0.16715984258853922
1103	74-75	0.1785888193967864
1103	76-77	0.1976935165466429
1103	78-79	0.2034049414614998
1103	80-81	0.19124626583431592
1103	82-83	0.1855454577506137
1103	84-85	0.1543517750217731
1103	86-87	0.14039159063430873
1103	88-89	0.11700462635216269
1103	90-91	0.21549590233403393
1103	92-93	0.19028645511411924
1103	94-95	0.11380775597145032
1103	96-97	0.11921595383624606
1103	98-99	0.13210436671073467
1103	100	0.05863184631599694
1104	1	0.12335919064652145
1104	2	0.08619874193023236
1104	3	0.0888264120678457
1104	4	0.08755427141741023
1104	5	0.13870178214374818
1104	6	0.08408934697929737
1104	7	0.02409951835802815
1104	8	0.10146919174050595
1104	9	0.12044959487435136
1104	10-11	0.18651141050522568
1104	12-13	0.20080566632204722
1104	14-15	0.24443768303922297
1104	16-17	0.21857522236137328
1104	18-19	0.18277875988682268
1104	20-21	0.1538784696603912
1104	22-23	0.1696547285814347
1104	24-25	0.15200447837818842
1104	26-27	0.1621306351036509
1104	28-29	0.2343586547378962
1104	30-31	0.29848007616837435
1104	32-33	0.29731364549851946
1104	34-35	0.2250877004066254
1104	36-37	0.264233593139771
1104	38-39	0.20844636978634412
1104	40-41	0.24861184511044598
1104	42-43	0.1537579517926062
1104	44-45	0.0471364786369719
1104	46-47	0.07769835204273079
1104	48-49	0.12782561271025372
1104	50-51	0.12656847647998148
1104	52-53	0.09173553476081509
1104	54-55	0.08641998684426255
1104	56-57	0.058674880506835336
1104	58-59	0.0857080756033568
1104	60-61	0.07457016519204274
1104	62-63	0.1326117373965161
1104	64-65	0.047221275731033074
1104	66-67	0.0421021580886034
1104	68-69	0.07417815100918546
1104	70-71	0.0727545589382288
1104	72-73	0.009957180061022086
1104	74-75	-0.01078753340225802
1104	76-77	0.001251811463220065
1104	78-79	0.0043673678674025496
1104	80-81	-0.018624193962232027
1104	82-83	-0.039066063078387
1104	84-85	0.04529301746160996
1104	86-87	0.02268393027857485
1104	88-89	0.03509184607410276
1104	90-91	0.10162067831233657
1104	92-93	0.0807079079062234
1104	94-95	0.060118287454940855
1104	96-97	0.028176114474860015
1104	98-99	-0.023469461897605015
1104	100	0.08911955660438764
1105	1	0.21075108015731914
1105	2	0.26058152161928305
1105	3	0.31502544242146513
1105	4	0.3381024866579665
1105	5	0.25066196213246883
1105	6	0.30111072726953836
1105	7	0.22923448976252558
1105	8	0.3161487942457555
1105	9	0.3426259271106318
1105	10-11	0.4630142658898464
1105	12-13	0.3776347779849445
1105	14-15	0.4860467581206578
1105	16-17	0.40577356165763234
1105	18-19	0.3493433022428647
1105	20-21	0.491402787534561
1105	22-23	0.40863734828730003
1105	24-25	0.35999927760567374
1105	26-27	0.33228903678251953
1105	28-29	0.34582171635391035
1105	30-31	0.35093843951069914
1105	32-33	0.35614669023173917
1105	34-35	0.3205272771801546
1105	36-37	0.36074287173391895
1105	38-39	0.4249647999961468
1105	40-41	0.3546372558049953
1105	42-43	0.3970376028676412
1105	44-45	0.32333369005317536
1105	46-47	0.35620630822772625
1105	48-49	0.3374865100129085
1105	50-51	0.3371851837394928
1105	52-53	0.3529814277459167
1105	54-55	0.31295985968803564
1105	56-57	0.2740020108470347
1105	58-59	0.2979570362055881
1105	60-61	0.2374470549256955
1105	62-63	0.29247648065489074
1105	64-65	0.34124705909947295
1105	66-67	0.2617219807297104
1105	68-69	0.24759793524432894
1105	70-71	0.25826947859881955
1105	72-73	0.2569914442598744
1105	74-75	0.26196438838844216
1105	76-77	0.22646983055150827
1105	78-79	0.23426522828325602
1105	80-81	0.27384969560771566
1105	82-83	0.2567322470315574
1105	84-85	0.2846307118015332
1105	86-87	0.2815824220462133
1105	88-89	0.23775146581620987
1105	90-91	0.2891235854880705
1105	92-93	0.2784688584301591
1105	94-95	0.24848418323927035
1105	96-97	0.18859077388854217
1105	98-99	0.19296468625026364
1105	100	0.162857404956938
1106	1	0.20345235197124012
1106	2	0.18756475311060683
1106	3	0.2505590189718063
1106	4	0.21290870327836586
1106	5	0.22318575563929954
1106	6	0.22002166271909118
1106	7	0.27489404214477275
1106	8	0.22276471082515314
1106	9	0.31859026444519145
1106	10-11	0.29545873770439357
1106	12-13	0.3358435215327802
1106	14-15	0.4164964973464862
1106	16-17	0.38176294959571067
1106	18-19	0.3271768451575454
1106	20-21	0.29297186861840174
1106	22-23	0.3599501303838366
1106	24-25	0.27626867910240094
1106	26-27	0.3275519285145947
1106	28-29	0.36415673581901586
1106	30-31	0.31121177029896785
1106	32-33	0.35020650063520975
1106	34-35	0.33122956041219
1106	36-37	0.362587091946466
1106	38-39	0.2838578816718069
1106	40-41	0.2789692984233767
1106	42-43	0.32244910469568566
1106	44-45	0.3619434546218656
1106	46-47	0.28878163030412196
1106	48-49	0.29518112906499283
1106	50-51	0.29649132638233056
1106	52-53	0.2792078570198413
1106	54-55	0.364227485462294
1106	56-57	0.2883312822424031
1106	58-59	0.32637753302689987
1106	60-61	0.3160493904247801
1106	62-63	0.3312631651109683
1106	64-65	0.337858704029081
1106	66-67	0.25485544069036337
1106	68-69	0.2867809280764675
1106	70-71	0.2195247408564569
1106	72-73	0.23806793131981152
1106	74-75	0.2014987550765639
1106	76-77	0.18432114634750718
1106	78-79	0.2298581486695639
1106	80-81	0.1871110676031762
1106	82-83	0.15776598888978555
1106	84-85	0.2077508662385057
1106	86-87	0.2137653499033476
1106	88-89	0.2522635935701132
1106	90-91	0.22574307960678652
1106	92-93	0.18512946163303212
1106	94-95	0.19663347857362368
1106	96-97	0.22049937154271504
1106	98-99	0.18964137141871618
1106	100	0.12289790865150962
1107	1	0.11279225578503826
1107	2	-0.022904341406633222
1107	3	0.0764549528850047
1107	4	0.05127204360327653
1107	5	0.09388088588089971
1107	6	0.08480965852290723
1107	7	0.10145941424731575
1107	8	0.16648108686887753
1107	9	0.18630947581464596
1107	10-11	0.17079029237302024
1107	12-13	0.07227119016273065
1107	14-15	0.12431374109126381
1107	16-17	0.11283677982317641
1107	18-19	0.10535499400844373
1107	20-21	0.060910706591762676
1107	22-23	0.09008852539929535
1107	24-25	0.1244256865000537
1107	26-27	0.19129004493706958
1107	28-29	0.13611294386721795
1107	30-31	0.16740509234121248
1107	32-33	0.18643084331445436
1107	34-35	0.13703293254807392
1107	36-37	0.1313985705700773
1107	38-39	0.1799363705518644
1107	40-41	0.15431434435232205
1107	42-43	0.17402517410189233
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2216	30-31	-0.35787482760281364
2216	32-33	-0.41181749598116113
2216	34-35	-0.3811377330291279
2216	36-37	-0.3400347534504533
2216	38-39	-0.4016864130813502
2216	40-41	-0.36918931466931326
2216	42-43	-0.32880280778309867
2216	44-45	-0.283269923821436
2216	46-47	-0.24000951827076733
2216	48-49	-0.24589411632116054
2216	50-51	-0.2562435019022473
2216	52-53	-0.18328106171435365
2216	54-55	-0.16602503452674
2216	56-57	-0.1635717492006208
2216	58-59	-0.18551638460697006
2216	60-61	-0.20497435793426177
2216	62-63	-0.23841025749795364
2216	64-65	-0.21946314630525876
2216	66-67	-0.23758401452383993
2216	68-69	-0.15156188455811304
2216	70-71	-0.15785031264827154
2216	72-73	-0.1615831142379136
2216	74-75	-0.1318476150400656
2216	76-77	-0.12198477846803257
2216	78-79	-0.1489162504310002
2216	80-81	-0.11281759789160617
2216	82-83	-0.13049594947229792
2216	84-85	-0.062497569020138144
2216	86-87	-0.12138109765601257
2216	88-89	-0.1895010376868811
2216	90-91	-0.18692196857307408
2216	92-93	-0.10939689181037693
2216	94-95	-0.15537896556558906
2216	96-97	-0.1129195000349128
2216	98-99	-0.16271072232719774
2216	100	-0.16016497208707392
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	86.0
1	40.0
2	41.0
3	20.0
4	39.0
5	44.0
6	164.0
7	721.0
8	2402.0
9	3142.0
10	3229.0
11	3635.0
12	4121.0
13	4885.0
14	6116.0
15	6772.0
16	7160.0
17	7732.0
18	8537.0
19	9400.0
20	10409.0
21	11585.0
22	13104.0
23	15583.0
24	19276.0
25	24488.0
26	26751.0
27	26072.0
28	28054.0
29	35113.0
30	39594.0
31	43689.0
32	54940.0
33	74240.0
34	115789.0
35	185075.0
36	362504.0
37	773624.0
38	738732.0
39	221421.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5409113173025	20.381945190162025	12.73663302681743	24.340510465718047
2	15.613554207377724	20.91846432366239	38.96645931594689	24.501522153012992
3	17.985995846671692	26.686450026070823	30.29956174700151	25.02799238025597
4	12.57333792189429	17.692191575651357	38.8790177894446	30.855452713009747
5	13.313556816285436	38.47197322691684	34.93210824180809	13.282361714989644
6	29.66844824249282	38.27153099072904	18.260673268780206	13.799347497997935
7	26.08988262256603	33.03438236205425	23.36546600451092	17.510269010868793
8	26.18908065893686	34.11531568085675	20.912101939053567	18.783501721152817
9	26.770072430426666	14.99162000632178	20.93340077488543	37.304906788366125
10-11	24.938669188909977	26.1191704570712	28.095327779689615	20.846832574329206
12-13	25.516457162976657	24.330684222543308	27.444461196969243	22.70839741751079
14-15	23.182260872816684	25.396276114165744	24.93964499172012	26.48181802129745
16-17	22.582545455049043	27.96316441093663	26.1086441107512	23.345646023263125
18-19	22.37266693890509	27.986969946504974	27.454616302211658	22.185746812378273
20-21	23.814113549575374	27.15288236169822	26.291878286820175	22.741125801906225
22-23	24.2182772291761	26.76775177632007	25.978810866052104	23.035160128451725
24-25	23.6839476887003	27.10063352611402	26.397885003559786	22.81753378162589
26-27	23.39499256270635	27.221360274344654	25.95645777446885	23.427189388480148
28-29	22.96951792863444	27.59426156294098	26.290847138882693	23.145373369541886
30-31	23.33321331185872	27.259127286928415	26.17067676878186	23.236982632431
32-33	23.40659489039849	27.6947866360007	25.427868797404173	23.470749676196636
34-35	23.76381221447255	27.436324890636172	25.986109452116214	22.813753442775063
36-37	23.702051455353285	26.945399321906198	26.036842265631062	23.31570695710946
38-39	23.561974575873585	26.90725527845715	26.3647501881438	23.166019957525467
40-41	23.34794333762856	26.468985812247166	26.754589842177673	23.4284810079466
42-43	23.308216132812177	26.710136125277362	26.290336878204716	23.691310863705745
44-45	23.19415532349698	27.2307925914473	26.4010676549077	23.173984430148025
46-47	23.41602775873174	27.071342987942128	25.935177682201306	23.577451571124826
48-49	23.654583726304633	26.791849560284813	26.072147192864687	23.48141952054587
50-51	23.5456663216397	26.814759304733148	25.92881109063728	23.710763282989873
52-53	23.26961481816485	27.060468814334186	26.026568061742122	23.64334830575884
54-55	23.086944867524554	26.955738316928446	26.047824560164067	23.90949225538293
56-57	23.817848719822408	26.74417920062061	25.802582866087846	23.63538921346914
58-59	23.09633120455303	26.65948415431804	26.536958943665066	23.70722569746385
60-61	23.173847491956387	27.12549906243707	26.13096721274289	23.569686232863653
62-63	23.611933811860915	26.609862077742406	25.817477886640734	23.960726223755945
64-65	23.01705471725393	26.823185550025237	26.048792819686124	24.110966913034705
66-67	23.439865214688552	26.568117317245903	26.053373875129655	23.938643592935893
68-69	23.1315075092256	26.516585474227284	26.347233739852534	24.004673276694586
70-71	23.33210446415445	26.430586409561148	26.05054894781035	24.186760178474056
72-73	23.63419732470427	26.55014222854503	26.15772373377808	23.657936712972617
74-75	23.559430478655937	27.00348483339899	26.17018973943836	23.266894948506717
76-77	23.61600958608079	26.67352953399457	25.873638970466008	23.836821909458635
78-79	23.51527092026554	26.751408721658905	25.92162136235358	23.811698995721976
80-81	23.398800965503124	26.8888706872413	26.237472970986246	23.47485537626933
82-83	23.894069339637262	26.14306471160375	26.930732570242576	23.032133378516413
84-85	23.886758148997878	26.798493571706857	25.923968659365393	23.390779619929873
86-87	23.194410319569855	27.092870622543337	26.22220583073912	23.490513227147687
88-89	23.756323503669048	26.3240940964703	26.077742150128895	23.841840249731757
90-91	23.54778805422014	26.359516184747406	26.29129515206451	23.80140060896795
92-93	23.962644824548278	26.382949766452235	26.295196202286164	23.35920920671332
94-95	22.984942457601203	26.396153381289082	26.799180474060293	23.819723687049418
96-97	23.356163766862235	26.33933425818637	26.38632722759488	23.918174747356517
98-99	23.04068504687396	26.887095947563218	26.117723731743535	23.954495273819283
100	23.493679267040147	26.107158464684854	25.867564144029465	24.531598124245534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	970.5
2	721.5
3	793.5
4	700.5
5	587.0
6	547.0
7	535.5
8	583.5
9	610.5
10	497.5
11	413.0
12	413.0
13	415.5
14	448.0
15	507.5
16	537.5
17	625.5
18	799.0
19	921.5
20	1122.5
21	1592.5
22	2288.0
23	2973.5
24	3938.5
25	5625.0
26	8135.5
27	10936.0
28	14212.5
29	19629.5
30	26945.5
31	33922.0
32	42613.0
33	49151.5
34	52870.5
35	57448.0
36	62945.0
37	71795.0
38	74777.5
39	74045.0
40	73681.0
41	72638.0
42	74377.0
43	78294.5
44	84496.0
45	98277.0
46	111796.0
47	119665.0
48	126133.5
49	123355.5
50	117995.0
51	120625.5
52	122215.5
53	120981.0
54	119266.0
55	112203.5
56	104495.0
57	89849.0
58	75251.5
59	70722.0
60	59546.0
61	45348.5
62	37245.5
63	29481.0
64	20518.5
65	13102.0
66	8951.0
67	6440.0
68	4683.5
69	3490.5
70	2875.5
71	2241.5
72	1739.5
73	1452.5
74	1189.5
75	965.5
76	818.0
77	694.0
78	549.0
79	413.0
80	288.0
81	217.5
82	186.0
83	147.0
84	93.5
85	75.5
86	64.0
87	45.0
88	35.0
89	26.0
90	20.5
91	15.0
92	16.0
93	19.5
94	14.5
95	9.5
96	10.5
97	10.5
98	8.5
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06148243284878992
2	0.009935505758785306
3	0.011908759515756604
4	0.009970124245749716
5	0.012324181359329509
6	0.012566510768080371
7	0.0029425713919747423
8	0.005504339427340989
9	0.007027552853774973
10-11	0.006785223445024112
12-13	0.006110162949218141
14-15	0.010489401550215846
16-17	0.009762413323963262
18-19	0.007979561245296213
20-21	0.006923697392881747
22-23	0.0025617680353662462
24-25	0.0039638167574248
26-27	0.005071608340285879
28-29	0.0061620906796647544
30-31	0.008931569636817453
32-33	0.010489401550215846
34-35	0.011597193133076925
36-37	0.018486272038994264
38-39	0.018919003126049373
40-41	0.01871129220426292
42-43	0.02430217784901493
44-45	0.027885191249831236
46-47	0.030810453398323775
48-49	0.031450895407165336
50-51	0.032299048337793346
52-53	0.03036041306778646
54-55	0.037249491973703794
56-57	0.03946507513942595
58-59	0.03673021466923767
60-61	0.037751460034687725
62-63	0.03297410883359932
64-65	0.03505121805146384
66-67	0.03721487348673939
68-69	0.039759332278623424
70-71	0.03986318773951666
72-73	0.03544933065155454
74-75	0.03868615918272676
76-77	0.038426520530493696
78-79	0.03749182138245466
80-81	0.04235571880095408
82-83	0.03872077766969117
84-85	0.034791579399230776
86-87	0.03293949034663491
88-89	0.030862381128770384
90-91	0.03314720126842136
92-93	0.03454924999047992
94-95	0.03832266506960047
96-97	0.033372221433690015
98-99	0.03067197945046614
100	0.02880258115438807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2888630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.659500424345296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20986215203102	31.826380920272257
2	14.387414782919633	13.426191723377409
3	6.231362005373827	8.722567144019676
4	3.411279523964931	6.366743935840084
5	2.0488465400782743	4.779907800310031
6	1.3647364020859392	3.8206751239349
7	0.8993856770637791	2.9375420466425237
8	0.6639374352000448	2.4783191231564174
9	0.4825903591895069	2.026568256244897
>10	2.1950867528607745	16.84354685353333
>50	0.07125988937943502	2.2182850594897743
>100	0.029428246393179928	2.4878674824313305
>500	0.002971026548554059	0.9511106008377205
>1k	0.0018392069110096554	1.1142939299098225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.7309243482204366E-5	0.0
16-17	0.0	0.0	0.0	3.461848696440873E-5	0.0
18-19	0.0	0.0	0.0	6.923697392881746E-5	0.0
20-21	0.0	0.0	0.0	1.038554608932262E-4	0.0
22-23	0.0	0.0	0.0	3.6349411312629166E-4	0.0
24-25	0.0	0.0	0.0	0.0014539764525051669	0.0
26-27	0.0	0.0	0.0	0.0036522503747451213	0.0
28-29	0.0	0.0	0.0	0.009520083915212401	0.0
30-31	0.0	0.0	0.0	0.02360980810972676	0.0
32-33	0.0	0.0	0.0	0.05154692709000461	0.0
34-35	0.0	0.0	0.0	0.09703561896123768	0.0
36-37	0.0	0.0	0.0	0.166428376081395	0.0
38-39	0.0	0.0	0.0	0.25827122199797137	0.0
40-41	0.0	0.0	0.0	0.3539913384545615	0.0
42-43	0.0	0.0	0.0	0.44398209531854205	0.0
44-45	0.0	0.0	0.0	0.5525283611954456	0.0
46-47	0.0	0.0	0.0	0.6834381696513572	0.0
48-49	0.0	0.0	0.0	0.8242834838660542	0.0
50-51	0.0	0.0	0.0	0.9705985190211277	0.0
52-53	0.0	0.0	0.0	1.1042258787037453	0.0
54-55	0.0	0.0	0.0	1.2450365744314777	0.0
56-57	0.0	0.0	0.0	1.4094570782689373	0.0
58-59	0.0	0.0	0.0	1.5910137331537788	0.0
60-61	0.0	0.0	0.0	1.7807749694491852	0.0
62-63	0.0	0.0	0.0	1.9648587738824288	0.0
64-65	0.0	0.0	0.0	2.151798603490236	0.0
66-67	0.0	0.0	0.0	2.358851774024364	0.0
68-69	0.0	0.0	0.0	2.589687845103042	0.0
70-71	0.0	0.0	0.0	2.8328827160280134	0.0
72-73	0.0	0.0	0.0	3.072633047500026	0.0
74-75	0.0	0.0	0.0	3.316329886485981	0.0
76-77	0.0	0.0	0.0	3.580728580676653	0.0
78-79	0.0	0.0	0.0	3.8606536662708617	0.0
80-81	0.0	0.0	0.0	4.160155506243444	0.0
82-83	0.0	0.0	0.0	4.473989399819292	0.0
84-85	0.0	0.0	0.0	4.778545538888677	0.0
86-87	0.0	0.0	0.0	5.09847228617026	0.0
88	0.0	0.0	0.0	5.344886676382922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1765	0.0	30.635612	1
GGTATCA	5775	0.0	26.949419	1
GTACATG	10870	0.0	26.083218	1
GCATCGT	1910	0.0	25.587343	8
TACATGG	11040	0.0	25.326818	2
GTATCAA	8080	0.0	24.964296	1
ACATGGG	11285	0.0	23.9029	3
TAGGCAT	2555	0.0	23.542864	5
CTAGGCA	2900	0.0	23.010742	4
GCTATTG	2085	0.0	22.988962	8
CATGGGG	9210	0.0	22.552925	4
CAACGCA	8230	0.0	22.09792	5
TAGTACT	2510	0.0	22.092686	4
ATCAACG	8360	0.0	21.979143	3
CTATTGA	2250	0.0	21.511957	9
GGCTATT	2385	0.0	21.278723	7
CTGTGCG	2820	0.0	20.996489	9
TATCAAC	9505	0.0	20.863783	2
GGGCTAT	2430	0.0	20.692371	6
AACGCAG	9260	0.0	20.603823	6
>>END_MODULE