FastQCFastQC Report
Mon 16 Jan 2017
ERR1146508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888630
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110220.3815649633217131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105080.36377106102200696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53860.18645517079030544No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT30950.10714421715484503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85100.046.8382071
GTATCAA146100.038.716911
CAACGCA174200.031.7680855
ATCAACG174500.031.6865253
TATCAAC181950.030.983372
GTACTAG16550.030.7094631
AACGCAG187200.030.25376
TCAACGC193550.028.640684
ACGCAGA195300.028.2504627
CGCAGAG198150.027.7967018
GCAGAGT215000.025.3777719
GTACATG101400.025.0148141
GAGTACT133900.024.88429812-13
TAGTACT23600.024.3058364
TACATGG103550.024.2214982
GTGATCG11050.023.8169128
ACATGGG103200.023.2811243
TAGGCAT24750.023.1764765
CTGTGCG27400.022.6403479
GTATAAG15550.022.394831
Produced by FastQC (version 0.11.5)