##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2888630
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.520701162834975	34.0	31.0	34.0	31.0	34.0
2	32.58949467394578	34.0	31.0	34.0	31.0	34.0
3	32.70487359059485	34.0	31.0	34.0	31.0	34.0
4	36.16782038544223	37.0	37.0	37.0	35.0	37.0
5	36.19553283044211	37.0	37.0	37.0	35.0	37.0
6	36.2756839747562	37.0	37.0	37.0	35.0	37.0
7	36.255379885966704	37.0	37.0	37.0	35.0	37.0
8	36.27115622284612	37.0	37.0	37.0	35.0	37.0
9	38.085316914938915	39.0	39.0	39.0	37.0	39.0
10-11	38.05022588562744	39.0	38.0	39.0	36.0	39.0
12-13	38.0204984715938	39.0	38.0	39.0	35.0	39.0
14-15	39.45348729328436	41.0	39.0	41.0	36.0	41.0
16-17	39.449075859490485	41.0	39.0	41.0	36.0	41.0
18-19	39.50404603566396	41.0	39.0	41.0	36.5	41.0
20-21	39.53499063569927	41.0	39.5	41.0	37.0	41.0
22-23	39.49125727420957	41.0	39.0	41.0	36.0	41.0
24-25	39.46112499697088	41.0	39.0	41.0	36.0	41.0
26-27	39.37110827624168	41.0	39.0	41.0	36.0	41.0
28-29	39.27548024496042	41.0	39.0	41.0	35.5	41.0
30-31	39.157576602057034	41.0	39.0	41.0	35.0	41.0
32-33	39.008638870329534	41.0	39.0	41.0	35.0	41.0
34-35	38.909320335245425	41.0	38.0	41.0	35.0	41.0
36-37	38.809691445425685	40.0	38.0	41.0	35.0	41.0
38-39	38.6835318126586	40.0	38.0	41.0	35.0	41.0
40-41	38.56855620138266	40.0	38.0	41.0	35.0	41.0
42-43	38.44534831390659	40.0	38.0	41.0	35.0	41.0
44-45	38.24798676189059	40.0	38.0	41.0	34.0	41.0
46-47	37.95371837168484	40.0	37.5	41.0	33.5	41.0
48-49	37.66495778275515	40.0	37.0	41.0	33.0	41.0
50-51	37.46158230718368	40.0	36.5	41.0	33.0	41.0
52-53	37.28349130902885	40.0	36.0	41.0	33.0	41.0
54-55	37.10078133925079	39.5	35.5	41.0	32.0	41.0
56-57	36.84635276930587	39.0	35.0	41.0	32.0	41.0
58-59	36.577751563890146	39.0	35.0	41.0	31.5	41.0
60-61	36.35043809695253	38.5	35.0	41.0	31.0	41.0
62-63	36.05701232071951	37.0	35.0	40.5	31.0	41.0
64-65	35.7398297808996	37.0	35.0	40.0	31.0	41.0
66-67	35.47174456403208	36.0	35.0	39.5	31.0	41.0
68-69	35.16271121604359	36.0	35.0	39.0	30.0	41.0
70-71	34.910677206841996	35.0	35.0	39.0	30.0	41.0
72-73	34.617738685812995	35.0	35.0	38.5	30.0	40.5
74-75	34.33436490654739	35.0	35.0	37.0	30.0	39.5
76-77	34.07881764019622	35.0	34.5	37.0	30.0	39.0
78-79	33.83915247020214	35.0	34.0	36.0	29.0	39.0
80-81	33.64891401806393	35.0	34.0	36.0	29.0	38.0
82-83	33.44441915371647	35.0	34.0	36.0	29.0	37.0
84-85	33.28401629838366	35.0	34.0	35.0	29.0	37.0
86-87	33.14048320484105	35.0	34.0	35.0	29.0	36.5
88-89	33.027998566794636	35.0	34.0	35.0	29.0	36.0
90-91	32.95675389371432	35.0	34.0	35.0	29.0	36.0
92-93	32.88587877298235	35.0	34.0	35.0	29.0	36.0
94-95	32.8326763898457	35.0	34.0	35.0	29.0	36.0
96-97	32.792604971907096	35.0	34.0	35.0	29.0	35.0
98-99	32.74558735455908	35.0	34.0	35.0	29.0	35.0
100	32.17355286069867	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2881214568748689
1101	2	0.2460242025353523
1101	3	0.25982952367636614
1101	4	0.20079327868749886
1101	5	0.1908543758745438
1101	6	0.20996348693082467
1101	7	0.18421659407584912
1101	8	0.1587755990196058
1101	9	0.19503318181600804
1101	10-11	0.22809850359003292
1101	12-13	0.22710723698952506
1101	14-15	0.3306382875444953
1101	16-17	0.35426240598490466
1101	18-19	0.3015334205815634
1101	20-21	0.3200993024147962
1101	22-23	0.3190782979709681
1101	24-25	0.369113197179729
1101	26-27	0.34549619292737077
1101	28-29	0.36185024334330507
1101	30-31	0.3665413672754241
1101	32-33	0.3195312733411626
1101	34-35	0.28108812159085517
1101	36-37	0.30329242888991104
1101	38-39	0.38151454733170453
1101	40-41	0.3851940562406426
1101	42-43	0.38478813804775314
1101	44-45	0.3380099400441594
1101	46-47	0.3260327228747215
1101	48-49	0.2769460186270436
1101	50-51	0.228744314173575
1101	52-53	0.27724120311194866
1101	54-55	0.2127072045157874
1101	56-57	0.23369305554291486
1101	58-59	0.2688186671155606
1101	60-61	0.2728756728197652
1101	62-63	0.3069962401135271
1101	64-65	0.28704860364031504
1101	66-67	0.31193298431278293
1101	68-69	0.37293494095096236
1101	70-71	0.37201610982435795
1101	72-73	0.34252811279453965
1101	74-75	0.25245382288944995
1101	76-77	0.32217882375528717
1101	78-79	0.25255307268962923
1101	80-81	0.19179391818391167
1101	82-83	0.19763828639139547
1101	84-85	0.1520430196272713
1101	86-87	0.21250935085571143
1101	88-89	0.27578262251972063
1101	90-91	0.20955661449107765
1101	92-93	0.26769313432690467
1101	94-95	0.25518502295636836
1101	96-97	0.16445044042202284
1101	98-99	0.10225632412753072
1101	100	0.16118237582201544
1102	1	0.29338146293908096
1102	2	0.3120282162694181
1102	3	0.2877649595438072
1102	4	0.17474631575294808
1102	5	0.18486894479074323
1102	6	0.17565757799451376
1102	7	0.19538934072927816
1102	8	0.1604876995668434
1102	9	0.18595783885833583
1102	10-11	0.22539981456964142
1102	12-13	0.23190624603091692
1102	14-15	0.3205833204724229
1102	16-17	0.2837761744786462
1102	18-19	0.2859840097184332
1102	20-21	0.30845189118446825
1102	22-23	0.2875262705982422
1102	24-25	0.29914681152784084
1102	26-27	0.3067226478450351
1102	28-29	0.28050091798509413
1102	30-31	0.3709771414801182
1102	32-33	0.3413739834726215
1102	34-35	0.35736051646581757
1102	36-37	0.41826598304333373
1102	38-39	0.35366224849698824
1102	40-41	0.4038503981293715
1102	42-43	0.43154327354414335
1102	44-45	0.402160563802596
1102	46-47	0.3535121238249488
1102	48-49	0.332402859233504
1102	50-51	0.2875686834050697
1102	52-53	0.2532354502033982
1102	54-55	0.2619245455253534
1102	56-57	0.24334957423930348
1102	58-59	0.2762849820356763
1102	60-61	0.19683231829360182
1102	62-63	0.19607533337838845
1102	64-65	0.275594583947381
1102	66-67	0.2420107788117889
1102	68-69	0.20434261457743474
1102	70-71	0.19784598499083472
1102	72-73	0.2315953231222636
1102	74-75	0.2127969359525892
1102	76-77	0.24255152315554795
1102	78-79	0.21346630557195567
1102	80-81	0.2227851840945334
1102	82-83	0.26825505609720324
1102	84-85	0.29041464285999297
1102	86-87	0.28899607387158
1102	88-89	0.33035612127166303
1102	90-91	0.303125821369882
1102	92-93	0.22520928358669323
1102	94-95	0.24333220666979827
1102	96-97	0.23114322923768782
1102	98-99	0.3136998711659871
1102	100	0.26294471551227616
1103	1	0.2107609814882352
1103	2	0.18410177169919706
1103	3	0.1482118966472683
1103	4	0.09039132425604635
1103	5	0.12601623969720066
1103	6	0.10129982909252533
1103	7	0.10427524352877526
1103	8	0.11545937349643509
1103	9	0.15169477185867208
1103	10-11	0.09526051943625902
1103	12-13	0.13002750819681097
1103	14-15	0.235127634544817
1103	16-17	0.1935531627458289
1103	18-19	0.156099922980232
1103	20-21	0.1978925634371933
1103	22-23	0.1441066562127773
1103	24-25	0.17834645326308163
1103	26-27	0.22929877003633692
1103	28-29	0.25778757696004817
1103	30-31	0.2555056087191119
1103	32-33	0.2664713089875903
1103	34-35	0.2867467860397639
1103	36-37	0.2669734576560714
1103	38-39	0.29882151007973334
1103	40-41	0.3355061258798173
1103	42-43	0.2842503460902819
1103	44-45	0.2758750803529608
1103	46-47	0.30658643259362606
1103	48-49	0.32649740293572904
1103	50-51	0.35182570456321116
1103	52-53	0.29573511047525614
1103	54-55	0.26563389029202966
1103	56-57	0.30700951494849704
1103	58-59	0.27616554120840675
1103	60-61	0.18399150170588996
1103	62-63	0.23826796022232344
1103	64-65	0.267853564974061
1103	66-67	0.25705879757006045
1103	68-69	0.278364675797107
1103	70-71	0.24324372418253404
1103	72-73	0.13919891711613985
1103	74-75	0.2283752851772789
1103	76-77	0.1893916144401615
1103	78-79	0.19855508511015785
1103	80-81	0.2567031219381377
1103	82-83	0.15633108494573378
1103	84-85	0.18448938832776207
1103	86-87	0.2049280784391101
1103	88-89	0.18871430753240048
1103	90-91	0.2672692479932195
1103	92-93	0.29738300109648463
1103	94-95	0.24808613300353954
1103	96-97	0.2700950805251594
1103	98-99	0.3250150857752132
1103	100	0.2956341485818754
1104	1	0.1937611110649442
1104	2	0.20695107216749875
1104	3	0.174855422009621
1104	4	0.14549016755583466
1104	5	0.10015405273443889
1104	6	0.0769243523050065
1104	7	0.07052781225004878
1104	8	0.11057697804293554
1104	9	0.11836618358773165
1104	10-11	0.11499906649815728
1104	12-13	0.1457422195327709
1104	14-15	0.1972833665801943
1104	16-17	0.1943667812574148
1104	18-19	0.18983617598696156
1104	20-21	0.1520792141166467
1104	22-23	0.24450419206537077
1104	24-25	0.17303027241779745
1104	26-27	0.20211093126626167
1104	28-29	0.26724291223521135
1104	30-31	0.18535647688302248
1104	32-33	0.24087751461669882
1104	34-35	0.19370409690340296
1104	36-37	0.20138106855365123
1104	38-39	0.2243065786458871
1104	40-41	0.20993934186410712
1104	42-43	0.25741336818401805
1104	44-45	0.2640709391910008
1104	46-47	0.2686815966450382
1104	48-49	0.24591221355299808
1104	50-51	0.2692312778214969
1104	52-53	0.2078255294642659
1104	54-55	0.20771230518568728
1104	56-57	0.1715639900898509
1104	58-59	0.15731530176525865
1104	60-61	0.22472124667703497
1104	62-63	0.22963388574674326
1104	64-65	0.2810763201032884
1104	66-67	0.29153178995240836
1104	68-69	0.2616828158056421
1104	70-71	0.20790624074462727
1104	72-73	0.24654431933318222
1104	74-75	0.22604381701847842
1104	76-77	0.2435335748444416
1104	78-79	0.2590132254472053
1104	80-81	0.22385873534695833
1104	82-83	0.13938744807440173
1104	84-85	0.1916234071400993
1104	86-87	0.18976269932687728
1104	88-89	0.22476697224993814
1104	90-91	0.16775319873217143
1104	92-93	0.12474291681687077
1104	94-95	0.09478788119403703
1104	96-97	0.15329411490495914
1104	98-99	0.10902246890980649
1104	100	0.20642423797154663
1105	1	0.18915253459451975
1105	2	0.19870698131893505
1105	3	0.16824587553181658
1105	4	0.11570624760130244
1105	5	0.11087877155240022
1105	6	0.1362706567448697
1105	7	0.14206426035403297
1105	8	0.15412671355416308
1105	9	0.14648848338555354
1105	10-11	0.14787306259395905
1105	12-13	0.16779444053171488
1105	14-15	0.2553655789412801
1105	16-17	0.2455881630194625
1105	18-19	0.23081405895763396
1105	20-21	0.22578252348582595
1105	22-23	0.2074763538530462
1105	24-25	0.16624244230322205
1105	26-27	0.1937788788258743
1105	28-29	0.20550421911813288
1105	30-31	0.20219483874521416
1105	32-33	0.2324144223147897
1105	34-35	0.24773278640620333
1105	36-37	0.22234189239068058
1105	38-39	0.24104573205131885
1105	40-41	0.2572392125806999
1105	42-43	0.2210646115110535
1105	44-45	0.23421860080810575
1105	46-47	0.2648260741497168
1105	48-49	0.30531659208247675
1105	50-51	0.24999181443303087
1105	52-53	0.2897090933097317
1105	54-55	0.29447586289952454
1105	56-57	0.3513078795362219
1105	58-59	0.26887267736902487
1105	60-61	0.2675067160607796
1105	62-63	0.3056151712411861
1105	64-65	0.26201284932354696
1105	66-67	0.20880582203818676
1105	68-69	0.23804315536099097
1105	70-71	0.2738077665276464
1105	72-73	0.2823946038441534
1105	74-75	0.2372774911873563
1105	76-77	0.27576792285326945
1105	78-79	0.2672743601559091
1105	80-81	0.22659571546930124
1105	82-83	0.28525178838954446
1105	84-85	0.2410423399459205
1105	86-87	0.24467190727405352
1105	88-89	0.2425441002088604
1105	90-91	0.26493780991069116
1105	92-93	0.23051763350348153
1105	94-95	0.22520548562013687
1105	96-97	0.2965037438320053
1105	98-99	0.2529214195773122
1105	100	0.2844190941276139
1106	1	0.1643236028174897
1106	2	0.1450363242129029
1106	3	0.16199627184018794
1106	4	0.08683490515250725
1106	5	0.11114676082134878
1106	6	0.050540185060270915
1106	7	0.027593440001560054
1106	8	0.04977894646319214
1106	9	0.10211812378152985
1106	10-11	0.10177943022253544
1106	12-13	0.09043537170440175
1106	14-15	0.14906282100029955
1106	16-17	0.13910926802246593
1106	18-19	0.13289288068162364
1106	20-21	0.15490099274781954
1106	22-23	0.21257336883878963
1106	24-25	0.18147836288550678
1106	26-27	0.15081546288382697
1106	28-29	0.18544168654909043
1106	30-31	0.1989497418137276
1106	32-33	0.19697929985517248
1106	34-35	0.21102204024526827
1106	36-37	0.25485946760706923
1106	38-39	0.3054165196031562
1106	40-41	0.26077852363169285
1106	42-43	0.23135936578420768
1106	44-45	0.32444043694950153
1106	46-47	0.3290922232430731
1106	48-49	0.32381894175080106
1106	50-51	0.26440876294073234
1106	52-53	0.29838005963415526
1106	54-55	0.29666381131511343
1106	56-57	0.3536711169405251
1106	58-59	0.36691760823588737
1106	60-61	0.32124032585535645
1106	62-63	0.2850361004463551
1106	64-65	0.3606385758236641
1106	66-67	0.24463483914383488
1106	68-69	0.30767476536067306
1106	70-71	0.26545769582492795
1106	72-73	0.21456696347792104
1106	74-75	0.27911806601520794
1106	76-77	0.28421199855515056
1106	78-79	0.24707385355964107
1106	80-81	0.2083637035640038
1106	82-83	0.18532956268516898
1106	84-85	0.19737744186925
1106	86-87	0.2551628912748072
1106	88-89	0.2424231527637204
1106	90-91	0.25986101806842044
1106	92-93	0.19968229764404555
1106	94-95	0.1995606478580214
1106	96-97	0.17707505676805368
1106	98-99	0.21336423335502985
1106	100	0.2654067298281717
1107	1	0.07655304439045807
1107	2	0.07279997956028694
1107	3	0.0880781196427236
1107	4	0.01883512496434747
1107	5	-0.011894212475830557
1107	6	0.04350700890886827
1107	7	0.04768942862074255
1107	8	0.042305282206015704
1107	9	0.01989627065659505
1107	10-11	0.026585227080580864
1107	12-13	0.04385841625890663
1107	14-15	0.10008658228957756
1107	16-17	0.11526465399908403
1107	18-19	0.06379709767296049
1107	20-21	0.1095499822616901
1107	22-23	0.11081280567591989
1107	24-25	0.07976237953933918
1107	26-27	0.07555698330255467
1107	28-29	0.11454156324452924
1107	30-31	0.08626875979816617
1107	32-33	0.08273762291312892
1107	34-35	0.05612045235172047
1107	36-37	0.09344660231653279
1107	38-39	0.1277241487410663
1107	40-41	0.07201127905336335
1107	42-43	-0.016232737537237085
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2216	30-31	-0.42905806746980346
2216	32-33	-0.4895689234950851
2216	34-35	-0.5214282734347506
2216	36-37	-0.5139973439908871
2216	38-39	-0.4282239734391311
2216	40-41	-0.41261119484850184
2216	42-43	-0.4582655576800505
2216	44-45	-0.4535903417211955
2216	46-47	-0.3817360172718267
2216	48-49	-0.4107631844765294
2216	50-51	-0.3775827584129843
2216	52-53	-0.41632815481796825
2216	54-55	-0.4517401359378752
2216	56-57	-0.3642926345627444
2216	58-59	-0.3670393108375336
2216	60-61	-0.3879522508885458
2216	62-63	-0.45920449132886887
2216	64-65	-0.42402076205254957
2216	66-67	-0.35051915935121514
2216	68-69	-0.41345736738698236
2216	70-71	-0.32514696597471726
2216	72-73	-0.3323690766382441
2216	74-75	-0.37426277050646206
2216	76-77	-0.34210755692019035
2216	78-79	-0.3294026131786154
2216	80-81	-0.3418317056858484
2216	82-83	-0.29178418130413775
2216	84-85	-0.3319896359740895
2216	86-87	-0.30935100770780366
2216	88-89	-0.3282018202287702
2216	90-91	-0.3600063502814379
2216	92-93	-0.3490315784307967
2216	94-95	-0.303197007314175
2216	96-97	-0.3368915553559404
2216	98-99	-0.44384377524300334
2216	100	-0.47932723775911157
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	74.0
9	121.0
10	269.0
11	250.0
12	178.0
13	230.0
14	400.0
15	694.0
16	1330.0
17	2624.0
18	4493.0
19	7039.0
20	9955.0
21	14474.0
22	21736.0
23	25498.0
24	24142.0
25	20195.0
26	17541.0
27	18153.0
28	21401.0
29	25426.0
30	30815.0
31	38256.0
32	50222.0
33	67222.0
34	100737.0
35	166701.0
36	313634.0
37	778141.0
38	841478.0
39	284495.0
40	691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02048738065011	20.12182265919464	13.10002108389208	23.75766887626317
2	15.911646355344592	20.880646626655018	39.03893737787999	24.168769640120402
3	17.775416783072014	27.015803086117803	30.9121367244418	24.296643406368386
4	12.616880666613586	17.923756244309587	38.44417595884554	31.015187130231286
5	12.793571733581022	38.24461116323238	34.92904061891666	14.03277648426994
6	29.230250992993913	38.58113261371784	17.823379506295165	14.365236886993088
7	25.1704961663461	33.772053722390005	23.31162060327602	17.745829507987875
8	26.30811145768063	33.42432225657145	20.76981129462756	19.49775499112036
9	26.8794895850282	13.896656892713846	21.14753360589622	38.07631991636173
10-11	24.945700903196325	25.638416827354142	28.05892412666212	21.35695814278741
12-13	26.14554304289577	23.960320290241395	27.862585377843473	22.03155128901936
14-15	23.387146049709735	24.55860123286839	26.36585965766811	25.688393059753768
16-17	21.72012338028754	27.27197668098718	27.46180715425652	23.54609278446876
18-19	21.52435583650381	26.10910708536573	30.50748970965475	21.859047368475714
20-21	22.88749337921437	25.342169125156218	29.809598321695752	21.960739173933664
22-23	23.139303240600903	25.050599500002686	29.519989766723885	22.29010749267253
24-25	22.698943789962716	25.400155090821602	29.901043055012238	21.999858064203444
26-27	22.44409979817422	25.540100324375224	29.45522617988458	22.560573697565975
28-29	21.970734213358657	25.85589911338562	29.858732291339596	22.314634381916125
30-31	22.46547320421543	25.531706077560752	29.658945117851875	22.34387560037194
32-33	22.725229408377118	25.921463256145817	28.863661501887698	22.48964583358937
34-35	22.91340563046623	25.756714741473214	29.347741985402216	21.98213764265834
36-37	22.892668643629488	25.333690368295297	29.23169514984044	22.541945838234774
38-39	22.8515440073378	25.278824179227087	29.530117234850685	22.339514578584428
40-41	22.59179475018413	25.00331045298248	29.765360285469235	22.639534511364158
42-43	22.803529861400563	25.179428736015772	29.119417907285577	22.897623495298088
44-45	22.703669873567414	25.79065153899906	28.946662837841465	22.559015749592064
46-47	23.17495243830394	25.657975020221617	28.03252006626702	23.134552475207425
48-49	23.527645192977598	25.725049656193065	27.757980691421917	22.989324459407413
50-51	23.334937912146973	25.954399843384802	27.349829511337383	23.36083273313085
52-53	22.955224221619314	26.256139234568526	27.52094185646784	23.267694687344324
54-55	22.82966419999865	26.185881330252464	27.286508818607903	23.697945651140984
56-57	23.624873035644185	25.969014119613405	26.941022763229043	23.465090081513367
58-59	22.67309642315225	26.04641638414304	27.79281223894765	23.48767495375706
60-61	22.86049449026776	26.427518386689886	27.339838165625522	23.372148957416826
62-63	23.17678790083906	26.00831822507605	27.072935407742925	23.741958466341963
64-65	22.57070585288516	26.140744057855663	27.332418555869083	23.9561315333901
66-67	22.92916882176298	25.936817769210474	27.33714569004972	23.796867718976827
68-69	22.724282832926782	25.759420574841492	27.64398560092061	23.872310991311117
70-71	22.85279370929443	25.683908939308914	27.267351870937233	24.195945480459425
72-73	23.17697032160324	25.759642220315413	27.318212008628063	23.745175449453285
74-75	23.180417420439106	26.346322251154696	27.032467938237716	23.440792390168482
76-77	23.292236775029448	26.00715417657297	26.666332008838427	24.03427703955915
78-79	23.04941975391556	26.231893490345847	26.756541745799524	23.962145009939064
80-81	22.92250060495827	26.418934380289055	26.96183899005376	23.696726024698915
82-83	23.499869920787557	25.60758589425521	27.650755575880492	23.241788609076742
84-85	23.51036954401355	26.19172946321393	26.636785116807204	23.661115875965315
86-87	22.748460039080832	26.55853807438374	26.905432803145164	23.787569083390267
88-89	23.244432904928818	25.86789562508114	26.782500324548874	24.10517114544117
90-91	22.979647791513624	25.848672207932484	27.029318396610158	24.142361603943737
92-93	23.411374942446766	25.944132685736836	26.828860047842753	23.81563232397365
94-95	22.493033029498413	25.906796647545722	27.375624430958624	24.224545891997245
96-97	22.891305999736552	25.76503266180702	27.00993571840657	24.33372562004986
98-99	22.59194759565612	26.253476795371345	26.752314387290383	24.40226122168215
100	23.012082269241326	25.480464153557435	26.46401577504498	25.04343780215626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	610.0
1	389.0
2	434.5
3	612.0
4	351.5
5	253.5
6	577.5
7	1431.5
8	2729.0
9	3437.5
10	2471.5
11	1274.5
12	1204.0
13	1413.0
14	1643.0
15	2009.0
16	2453.5
17	2962.0
18	3560.5
19	4219.0
20	4902.0
21	5651.5
22	6507.5
23	7264.0
24	8275.0
25	9951.0
26	12362.5
27	14908.5
28	17903.0
29	23207.0
30	29675.0
31	35472.5
32	43476.0
33	49440.5
34	51948.5
35	55488.5
36	60751.5
37	69274.0
38	71916.0
39	71230.0
40	71370.5
41	70537.0
42	72033.0
43	75631.0
44	82199.5
45	96526.5
46	109868.5
47	117579.0
48	124447.5
49	121274.5
50	115616.5
51	117423.0
52	117972.0
53	117666.0
54	117002.5
55	110720.0
56	102963.0
57	88473.0
58	74235.5
59	69682.5
60	59013.0
61	44410.5
62	35647.5
63	28100.5
64	19587.5
65	12311.5
66	8219.0
67	5893.5
68	4255.0
69	3083.0
70	2391.5
71	1846.5
72	1445.0
73	1268.5
74	1076.5
75	844.0
76	683.0
77	564.0
78	438.0
79	330.5
80	227.0
81	139.5
82	98.0
83	73.0
84	48.0
85	28.5
86	17.5
87	13.5
88	8.5
89	4.0
90	1.5
91	0.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005850524296985076
2	0.09128895012514583
3	5.885142783949484E-4
4	0.0
5	0.03922274573067509
6	0.004950443635910449
7	6.923697392881746E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.7309243482204366E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.019680609839267E-4
24-25	0.0
26-27	0.0
28-29	3.461848696440873E-5
30-31	1.3847394785763492E-4
32-33	0.0020251814874179108
34-35	0.0014366672090229625
36-37	8.308436871458096E-4
38-39	9.173899045568314E-4
40-41	0.0018174705656314586
42-43	6.231327653593573E-4
44-45	5.365865479483354E-4
46-47	4.673495740195179E-4
48-49	6.058235218771528E-4
50-51	7.442974697347877E-4
52-53	0.0012808840176831231
54-55	0.001748233591702641
56-57	8.308436871458096E-4
58-59	0.0028040974441171074
60-61	0.00205979997438232
62-63	0.0011424100698254882
64-65	0.004552331035819749
66-67	0.004431166331444318
68-69	0.0038772705400137784
70-71	0.0017655428351848455
72-73	0.005140845314214697
74-75	0.003583013400816304
76-77	0.0037214873486739386
78-79	9.693176350034446E-4
80-81	3.461848696440873E-5
82-83	1.9040167830424804E-4
84-85	8.654621741102184E-5
86-87	5.19277304466131E-5
88-89	1.7309243482204368E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.981126000907005E-4
98-99	5.019680609839267E-4
100	5.885142783949484E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2888630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.21514585023194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.81032206321825	27.359771984992637
2	15.825978156707066	13.361919522159473
3	6.9456654143341625	8.796368354790848
4	3.761310713781158	6.351371214812464
5	2.245812141525271	4.7403643553355534
6	1.460354206748269	3.698943949852677
7	1.0259510773930967	3.031747205714652
8	0.7234651383397603	2.4432949066056966
9	0.5616936394303385	2.1340781020529396
>10	2.4977475845787045	17.411379680402483
>50	0.09281576585851659	2.679012627162026
>100	0.04050846916200035	3.1737602901574133
>500	0.005177661516370076	1.5331159276344242
>1k	0.0027411149204312167	1.5545234923737006
>5k	1.5228416224617869E-4	0.34624968073796575
>10k+	3.0456832449235737E-4	1.3840987052150766
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11022	0.3815649633217131	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10508	0.36377106102200696	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5386	0.18645517079030544	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	3095	0.10714421715484503	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	8.654621741102184E-5	0.0
16-17	0.0	0.0	0.0	1.038554608932262E-4	0.0
18-19	0.0	0.0	0.0	1.3847394785763492E-4	0.0
20-21	0.0	0.0	0.0	1.7309243482204368E-4	0.0
22-23	0.0	0.0	0.0	4.673495740195179E-4	0.0
24-25	0.0	0.0	0.0	0.0015232134264339843	0.0
26-27	0.0	0.0	0.0	0.0036695596182273254	0.0
28-29	0.0	0.0	0.0	0.009485465428247993	0.0
30-31	0.0	0.0	0.0	0.023679045083655575	0.0
32-33	0.0	0.0	0.0	0.05184118422920208	0.0
34-35	0.0	0.0	0.0	0.09779722567445467	0.0
36-37	0.0	0.0	0.0	0.16713805506416535	0.0
38-39	0.0	0.0	0.0	0.2589289732502951	0.0
40-41	0.0	0.0	0.0	0.35539338717662006	0.0
42-43	0.0	0.0	0.0	0.44614575075381757	0.0
44-45	0.0	0.0	0.0	0.5552978401525983	0.0
46-47	0.0	0.0	0.0	0.6871942754869955	0.0
48-49	0.0	0.0	0.0	0.8289742888497316	0.0
50-51	0.0	0.0	0.0	0.9758432197962356	0.0
52-53	0.0	0.0	0.0	1.110024475270284	0.0
54-55	0.0	0.0	0.0	1.2518737256069485	0.0
56-57	0.0	0.0	0.0	1.4174193302707512	0.0
58-59	0.0	0.0	0.0	1.5999799212775607	0.0
60-61	0.0	0.0	0.0	1.7909008768862749	0.0
62-63	0.0	0.0	0.0	1.9757809065196996	3.461848696440873E-5
64-65	0.0	0.0	0.0	2.163482342840724	3.461848696440873E-5
66-67	0.0	0.0	0.0	2.371625995714231	3.461848696440873E-5
68-69	0.0	0.0	0.0	2.60427953735854	3.461848696440873E-5
70-71	0.0	0.0	0.0	2.849603445231823	3.461848696440873E-5
72-73	0.0	0.0	0.0	3.0913962674347353	3.461848696440873E-5
74-75	0.0	0.0	0.0	3.3367028660645355	3.461848696440873E-5
76-77	0.0	0.0	0.0	3.6025555367077127	3.461848696440873E-5
78-79	0.0	0.0	0.0	3.8843846390849643	3.461848696440873E-5
80-81	0.0	0.0	0.0	4.1870886891017545	3.461848696440873E-5
82-83	0.0	0.0	0.0	4.503328567521628	3.461848696440873E-5
84-85	0.0	0.0	0.0	4.810186835974147	3.461848696440873E-5
86-87	0.0	0.0	0.0	5.132173383230112	3.461848696440873E-5
88	0.0	0.0	0.0	5.380474480982334	3.461848696440873E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8510	0.0	46.838207	1
GTATCAA	14610	0.0	38.71691	1
CAACGCA	17420	0.0	31.768085	5
ATCAACG	17450	0.0	31.686525	3
TATCAAC	18195	0.0	30.98337	2
GTACTAG	1655	0.0	30.709463	1
AACGCAG	18720	0.0	30.2537	6
TCAACGC	19355	0.0	28.64068	4
ACGCAGA	19530	0.0	28.250462	7
CGCAGAG	19815	0.0	27.796701	8
GCAGAGT	21500	0.0	25.377771	9
GTACATG	10140	0.0	25.014814	1
GAGTACT	13390	0.0	24.884298	12-13
TAGTACT	2360	0.0	24.305836	4
TACATGG	10355	0.0	24.221498	2
GTGATCG	1105	0.0	23.816912	8
ACATGGG	10320	0.0	23.281124	3
TAGGCAT	2475	0.0	23.176476	5
CTGTGCG	2740	0.0	22.640347	9
GTATAAG	1555	0.0	22.39483	1
>>END_MODULE