FastQCFastQC Report
Mon 16 Jan 2017
ERR1146507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289839
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.033.4434131
GTATCAA66300.031.1246171
ATCAACG69900.027.4958443
CAACGCA71450.026.8981385
GTACATG100950.026.168751
TCAACGC76900.025.2985124
TATCAAC81450.024.9814592
AACGCAG77550.024.9637586
TACATGG103500.024.2903842
ACATGGG101200.023.913753
ACGCAGA84400.022.6586137
CATGGGG72800.022.3339564
CGCAGAG85050.022.2108978
GTACTAG12850.021.948311
GCAGAGT92600.019.4357879
ATGGGGG46750.018.9968385
TGCGCGA7700.018.92361894
GTGTAAG15700.018.8622671
GTATAAG15550.018.439641
GAGTACA76550.018.1760481
Produced by FastQC (version 0.11.5)