FastQCFastQC Report
Mon 16 Jan 2017
ERR1146507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289839
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119280.3625709343223179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106910.32497031009724187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.1833524376116886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.054.1215361
GTATCAA134700.041.9829331
ATCAACG165850.033.3945123
CAACGCA167300.033.0769775
TATCAAC171000.032.766132
TCAACGC171550.032.3123364
AACGCAG175400.031.965726
ACGCAGA194200.028.5807957
CGCAGAG194750.028.403558
GTACATG97050.026.177861
GCAGAGT212200.025.9127869
TACATGG97250.025.493562
ACATGGG94150.024.9187073
GTACTAG12800.024.6263891
GAGTACT150900.023.34296612-13
CATGGGG70700.022.8762234
AGAGTAC191250.022.54645310-11
CAGAGTA209400.022.26427710-11
CTAGTAC18300.022.094933
GTACTTT161250.021.49492514-15
Produced by FastQC (version 0.11.5)