FastQCFastQC Report
Mon 16 Jan 2017
ERR1146506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941602
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.038.8363951
GTATCAA73050.033.984211
CAACGCA80750.028.2841455
ATCAACG81950.028.0420133
GTACTAG17000.027.3810271
TATCAAC90650.027.0610492
AACGCAG92900.025.1410036
GCATCGT16000.024.3776958
GTACATG129500.023.8538821
TCAACGC97650.023.5815944
TACATGG126050.023.5249352
ACGCAGA96600.023.3019857
CGCAGAG96350.023.1673568
ACATGGG123700.022.9465053
CTATTGA22150.022.2766639
GCTATTG24200.021.5547088
CATCGTC19650.020.5669779
GGGCTAT26700.020.4168476
GGCTATT25000.020.301047
GCAGAGT105700.020.1399219
Produced by FastQC (version 0.11.5)