##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146506_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2941602
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32105600961653	31.0	31.0	34.0	28.0	34.0
2	31.530345029681104	33.0	31.0	34.0	28.0	34.0
3	31.712763657354053	33.0	31.0	34.0	28.0	34.0
4	35.24298868439714	37.0	35.0	37.0	32.0	37.0
5	35.35925288329285	37.0	35.0	37.0	33.0	37.0
6	35.32768641033015	37.0	35.0	37.0	33.0	37.0
7	35.24031429132833	37.0	35.0	37.0	32.0	37.0
8	35.22256919868833	37.0	35.0	37.0	32.0	37.0
9	36.833493790118446	39.0	38.0	39.0	33.0	39.0
10-11	36.8124562738263	39.0	38.0	39.0	33.0	39.0
12-13	36.769943384591116	39.0	38.0	39.0	33.0	39.0
14-15	38.20430143166887	41.0	39.0	41.0	33.0	41.0
16-17	38.23904610480956	41.0	39.0	41.0	33.0	41.0
18-19	38.29108679556242	41.0	39.0	41.0	33.0	41.0
20-21	38.39821141677223	41.0	39.0	41.0	33.5	41.0
22-23	38.448302489595804	41.0	39.0	41.0	34.0	41.0
24-25	38.52156494998303	41.0	39.0	41.0	33.5	41.0
26-27	38.560881961597794	41.0	39.0	41.0	33.5	41.0
28-29	38.5369064203791	41.0	39.0	41.0	33.0	41.0
30-31	38.52927894392239	41.0	39.0	41.0	33.0	41.0
32-33	38.53821880050394	41.0	38.5	41.0	33.0	41.0
34-35	38.486819256989904	40.0	38.0	41.0	33.0	41.0
36-37	38.397235757930545	40.0	38.0	41.0	33.0	41.0
38-39	38.34958009275218	40.0	38.0	41.0	33.0	41.0
40-41	38.28569789522852	40.0	38.0	41.0	33.0	41.0
42-43	38.16719325048052	40.0	38.0	41.0	33.0	41.0
44-45	38.03823902757749	40.0	38.0	41.0	33.0	41.0
46-47	37.93513466471671	40.0	37.5	41.0	32.5	41.0
48-49	37.815201206689416	40.0	37.0	41.0	32.0	41.0
50-51	37.680108831854206	40.0	37.0	41.0	32.0	41.0
52-53	37.54271651977392	40.0	36.0	41.0	32.0	41.0
54-55	37.35976943855763	40.0	36.0	41.0	31.0	41.0
56-57	37.212669321002636	39.5	35.0	41.0	31.0	41.0
58-59	36.9783781082553	39.0	35.0	41.0	31.0	41.0
60-61	36.79400561326787	39.0	35.0	41.0	31.0	41.0
62-63	36.55157053877445	38.5	35.0	41.0	31.0	41.0
64-65	36.30150662802106	37.5	35.0	40.0	31.0	41.0
66-67	36.03989764760834	37.0	35.0	40.0	30.0	41.0
68-69	35.74829599653522	36.5	35.0	40.0	30.0	41.0
70-71	35.497159201006795	36.0	35.0	39.0	30.0	41.0
72-73	35.188703298406786	35.5	35.0	39.0	30.0	41.0
74-75	34.867465585079145	35.0	34.0	38.5	30.0	40.5
76-77	34.56373686854985	35.0	34.0	37.0	29.5	39.5
78-79	34.28394204926431	35.0	34.0	37.0	29.0	39.0
80-81	34.004711208382375	35.0	34.0	36.5	29.0	39.0
82-83	33.74304902566696	35.0	34.0	36.0	29.0	37.5
84-85	33.51564113704029	35.0	34.0	36.0	29.0	37.0
86-87	33.312435706801935	35.0	34.0	35.0	28.5	37.0
88-89	33.17182219076544	35.0	34.0	35.0	28.0	36.0
90-91	32.99648864802241	35.0	34.0	35.0	28.0	36.0
92-93	32.89572875596359	35.0	34.0	35.0	28.0	36.0
94-95	32.793789574524354	35.0	34.0	35.0	27.5	36.0
96-97	32.705163376962616	35.0	34.0	35.0	27.0	35.5
98-99	32.62880430459322	35.0	33.5	35.0	27.0	35.0
100	31.737626640177698	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29889579871537464
1101	2	0.3144108172429938
1101	3	0.41663332650500706
1101	4	0.35770886538966096
1101	5	0.34770435066820937
1101	6	0.3948477290109409
1101	7	0.4166219447051418
1101	8	0.41434243533232973
1101	9	0.5686779350431692
1101	10-11	0.5629192679418153
1101	12-13	0.5336334114228691
1101	14-15	0.6526106444093145
1101	16-17	0.6376412807209988
1101	18-19	0.5874421245218926
1101	20-21	0.5373748875367212
1101	22-23	0.4963851632827314
1101	24-25	0.5303523133596499
1101	26-27	0.5444838095860973
1101	28-29	0.5585013973937194
1101	30-31	0.5812745179607504
1101	32-33	0.5298612049407581
1101	34-35	0.505114656138943
1101	36-37	0.5470874947563615
1101	38-39	0.5057433148664217
1101	40-41	0.5173746887529305
1101	42-43	0.4659380494161738
1101	44-45	0.458535458367372
1101	46-47	0.4764359842362538
1101	48-49	0.42011690782144484
1101	50-51	0.472595908690181
1101	52-53	0.48846039922369755
1101	54-55	0.501518619683111
1101	56-57	0.43294703720054883
1101	58-59	0.45929140519467637
1101	60-61	0.4484434496424754
1101	62-63	0.43984815238134445
1101	64-65	0.40515391848299487
1101	66-67	0.38923875049813716
1101	68-69	0.43926169951951266
1101	70-71	0.390947288063515
1101	72-73	0.3741721162346252
1101	74-75	0.3783095195729942
1101	76-77	0.3533744069655995
1101	78-79	0.3247021541868378
1101	80-81	0.358338293910748
1101	82-83	0.3407328988385885
1101	84-85	0.25186300610743473
1101	86-87	0.3344101694961594
1101	88-89	0.2572077093959635
1101	90-91	0.23574536766081167
1101	92-93	0.24270126558249672
1101	94-95	0.18708358488719767
1101	96-97	0.1887892753628364
1101	98-99	0.1584629917607714
1101	100	0.2119059680089883
1102	1	0.24813185677496108
1102	2	0.22473358271281896
1102	3	0.2972992292297363
1102	4	0.22824617122108748
1102	5	0.3249070499297346
1102	6	0.29221212931651763
1102	7	0.29045056706317496
1102	8	0.36581302356762535
1102	9	0.42056869134568586
1102	10-11	0.4756595913439057
1102	12-13	0.4224496197243681
1102	14-15	0.4389822385015023
1102	16-17	0.46963612411290967
1102	18-19	0.44863864858606206
1102	20-21	0.48445121806893354
1102	22-23	0.4607997927708922
1102	24-25	0.44025172767083376
1102	26-27	0.445357568180448
1102	28-29	0.47240354916352345
1102	30-31	0.43522473441096565
1102	32-33	0.43473292635659533
1102	34-35	0.4672931639056941
1102	36-37	0.5022950323168445
1102	38-39	0.4579189578508931
1102	40-41	0.46793013820543194
1102	42-43	0.39288291827279664
1102	44-45	0.33227654571139453
1102	46-47	0.31612913422351596
1102	48-49	0.3219392276584685
1102	50-51	0.3117415669534864
1102	52-53	0.35203469257740494
1102	54-55	0.35013397491611187
1102	56-57	0.28563356635766723
1102	58-59	0.3481107965875978
1102	60-61	0.28037144811459314
1102	62-63	0.2611748648845307
1102	64-65	0.2776838395423269
1102	66-67	0.28389434000666824
1102	68-69	0.26501131754853446
1102	70-71	0.3011586453336932
1102	72-73	0.32913933039301213
1102	74-75	0.24893754350984665
1102	76-77	0.2773685500773837
1102	78-79	0.28140097007683096
1102	80-81	0.31840322897568285
1102	82-83	0.2833624506593111
1102	84-85	0.325976515147417
1102	86-87	0.28248758227945103
1102	88-89	0.29559260881027427
1102	90-91	0.3204585711240142
1102	92-93	0.32514810795581184
1102	94-95	0.2965310002169659
1102	96-97	0.28897389466510504
1102	98-99	0.22350309871264074
1102	100	0.10431747806756064
1103	1	0.18889868101692286
1103	2	0.13310056913417867
1103	3	0.17550310680212888
1103	4	0.09421853971937821
1103	5	0.14593549635700498
1103	6	0.13135151957263247
1103	7	0.19730143798609845
1103	8	0.20632055069440014
1103	9	0.21956940037697592
1103	10-11	0.19903059628065023
1103	12-13	0.28633629528845717
1103	14-15	0.24542730843201355
1103	16-17	0.19666731602212195
1103	18-19	0.19788879188865138
1103	20-21	0.25710025707831363
1103	22-23	0.2592131554208308
1103	24-25	0.2906643988426083
1103	26-27	0.2835565351932203
1103	28-29	0.3144552120490758
1103	30-31	0.3185746281138222
1103	32-33	0.32469693237185027
1103	34-35	0.32976224450159464
1103	36-37	0.28244871715013176
1103	38-39	0.2788924788127787
1103	40-41	0.24342760393654572
1103	42-43	0.26216700098083834
1103	44-45	0.22912540665723924
1103	46-47	0.21183779718158746
1103	48-49	0.18052413904682396
1103	50-51	0.23584831414584784
1103	52-53	0.19625337549423705
1103	54-55	0.2275658745329423
1103	56-57	0.18897510649792792
1103	58-59	0.12791129665032486
1103	60-61	0.0787035196369743
1103	62-63	0.08532532224424472
1103	64-65	0.11488850096262126
1103	66-67	0.1481556381784941
1103	68-69	0.2019564876182116
1103	70-71	0.1572549331635642
1103	72-73	0.1801723679705205
1103	74-75	0.12962241910132377
1103	76-77	0.15069006814100305
1103	78-79	0.18368540183314508
1103	80-81	0.23312020348393503
1103	82-83	0.1593140244555471
1103	84-85	0.15601694633281937
1103	86-87	0.1905063117552359
1103	88-89	0.15560792031788395
1103	90-91	0.19097320726780964
1103	92-93	0.18460225889660364
1103	94-95	0.13703118956054539
1103	96-97	0.09428538500051786
1103	98-99	0.060535753862154706
1103	100	0.01995771835549931
1104	1	0.08934660096917568
1104	2	0.07272377687852583
1104	3	0.14343992295430397
1104	4	0.10053837576166558
1104	5	0.16932221163332883
1104	6	0.09528199385358249
1104	7	0.1172966261951629
1104	8	0.15181643344434548
1104	9	0.16645835918077267
1104	10-11	0.23471666992714546
1104	12-13	0.2295571289319085
1104	14-15	0.23314229622878457
1104	16-17	0.24968722077954908
1104	18-19	0.17176209186786906
1104	20-21	0.17714427551224787
1104	22-23	0.16567684590470577
1104	24-25	0.21648073568501758
1104	26-27	0.2489609033780411
1104	28-29	0.2995826972146034
1104	30-31	0.2523740243632062
1104	32-33	0.2718215544285698
1104	34-35	0.2756629155027781
1104	36-37	0.20466622980571714
1104	38-39	0.22403952669116478
1104	40-41	0.23877871355764313
1104	42-43	0.206690073811302
1104	44-45	0.1249500662736267
1104	46-47	0.1120212949754702
1104	48-49	0.13581419324537336
1104	50-51	0.15341181041391394
1104	52-53	0.10499577651933123
1104	54-55	0.140933133610865
1104	56-57	0.11397347882376607
1104	58-59	0.12960128177424934
1104	60-61	0.13103021494449507
1104	62-63	0.10217174982233246
1104	64-65	0.13305753861907732
1104	66-67	0.04746488521843162
1104	68-69	0.10671631281199723
1104	70-71	0.06964362388377054
1104	72-73	0.10842019703930106
1104	74-75	0.043638184839799976
1104	76-77	0.04343512829970564
1104	78-79	0.018724420078669368
1104	80-81	0.0763927565570981
1104	82-83	0.042791028049208535
1104	84-85	0.12922582905333968
1104	86-87	0.11143067223907366
1104	88-89	0.08479657168098953
1104	90-91	0.11967721781699936
1104	92-93	0.04521014094035536
1104	94-95	-0.001195893153401073
1104	96-97	0.020470761176994756
1104	98-99	-0.05097097711972509
1104	100	0.006168089482518013
1105	1	-0.008327109790389642
1105	2	0.05796905291637344
1105	3	0.06324516272885816
1105	4	0.11554238564495733
1105	5	0.09832512122014236
1105	6	0.103313262226159
1105	7	0.08930153878648639
1105	8	0.15769844311261494
1105	9	0.09964670553008403
1105	10-11	0.18323016793043934
1105	12-13	0.11127652863107329
1105	14-15	0.2524867747453996
1105	16-17	0.14269555581595483
1105	18-19	0.15419439603011398
1105	20-21	0.13058101911894937
1105	22-23	0.13707143779253528
1105	24-25	0.18105259347169778
1105	26-27	0.1726216604711297
1105	28-29	0.1810621334869964
1105	30-31	0.26999723141750565
1105	32-33	0.24176698272575692
1105	34-35	0.2180587989798397
1105	36-37	0.2633593226364539
1105	38-39	0.22400592938998898
1105	40-41	0.23521449392064397
1105	42-43	0.1699104120556143
1105	44-45	0.190729041796871
1105	46-47	0.18877466245415064
1105	48-49	0.2274523758577871
1105	50-51	0.17741002969641784
1105	52-53	0.15300123490033002
1105	54-55	0.14799480203225812
1105	56-57	0.1035390651142265
1105	58-59	0.1027420252879736
1105	60-61	0.11941826123055677
1105	62-63	0.11551998784667461
1105	64-65	0.15894132319342447
1105	66-67	0.10880568752440212
1105	68-69	0.13476412679602845
1105	70-71	0.11648516278787469
1105	72-73	0.1255926554367619
1105	74-75	0.12324962327404876
1105	76-77	0.08906837507508669
1105	78-79	0.07711168666520507
1105	80-81	0.07121247646578865
1105	82-83	0.05505640386922295
1105	84-85	0.12993651791060756
1105	86-87	0.0557847638213147
1105	88-89	0.039576904656279055
1105	90-91	0.044067187684760256
1105	92-93	0.019581998533311662
1105	94-95	0.05551713819246373
1105	96-97	0.08221212806290623
1105	98-99	0.12867948261243356
1105	100	0.02236574186050433
1106	1	0.1967752321789007
1106	2	0.19226223950203902
1106	3	0.2447155453080221
1106	4	0.22581948992116452
1106	5	0.23943512748597584
1106	6	0.19000494682764923
1106	7	0.1697307938786139
1106	8	0.19098069627050762
1106	9	0.2502635270264406
1106	10-11	0.33085630191033744
1106	12-13	0.34287710890185963
1106	14-15	0.4083213794075462
1106	16-17	0.37691121404644434
1106	18-19	0.356433523898545
1106	20-21	0.33686989680803947
1106	22-23	0.34716533450615117
1106	24-25	0.3296441427674708
1106	26-27	0.34680755598606794
1106	28-29	0.4029790476087456
1106	30-31	0.38575137912775403
1106	32-33	0.34138229224892314
1106	34-35	0.30007165302221495
1106	36-37	0.3399101167629155
1106	38-39	0.322826204360247
1106	40-41	0.3354340251635435
1106	42-43	0.37203739056107565
1106	44-45	0.26364848949531705
1106	46-47	0.24912407355648725
1106	48-49	0.2882709299936934
1106	50-51	0.33303600021975654
1106	52-53	0.2699059351845605
1106	54-55	0.24039600162180363
1106	56-57	0.21779928796000547
1106	58-59	0.23706688158240752
1106	60-61	0.21652053963340023
1106	62-63	0.26983592213714047
1106	64-65	0.2548087099770555
1106	66-67	0.24046177491917575
1106	68-69	0.21661602153000814
1106	70-71	0.22353990257411027
1106	72-73	0.16669687261604338
1106	74-75	0.22713285172275732
1106	76-77	0.18948518930670843
1106	78-79	0.19013100178221265
1106	80-81	0.20790234488325154
1106	82-83	0.17328112939841134
1106	84-85	0.2087287101355244
1106	86-87	0.18325125522622443
1106	88-89	0.11041716219116893
1106	90-91	0.1577775608197669
1106	92-93	0.1377125095166889
1106	94-95	0.16452075693911894
1106	96-97	0.17642587930018294
1106	98-99	0.12520954953881613
1106	100	0.15354008644509776
1107	1	0.05060357860152109
1107	2	0.07481562432711897
1107	3	0.14302259188858102
1107	4	0.13128345651643514
1107	5	0.12562971436206993
1107	6	0.13274417791308224
1107	7	0.11026972639846377
1107	8	0.10571317717745643
1107	9	0.1632338219140479
1107	10-11	0.22183767401391918
1107	12-13	0.21142549599863258
1107	14-15	0.18542215611077495
1107	16-17	0.12427496645336333
1107	18-19	0.11418378892959424
1107	20-21	0.14628877567262322
1107	22-23	0.13603457143309328
1107	24-25	0.15778070407077394
1107	26-27	0.1822108358804897
1107	28-29	0.19829989743890764
1107	30-31	0.16733442579484858
1107	32-33	0.137537178556002
1107	34-35	0.056104084168225654
1107	36-37	0.09140618997638938
1107	38-39	0.09926007640974888
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2216	40-41	-0.367631533338475
2216	42-43	-0.32051783977683357
2216	44-45	-0.2896697583774639
2216	46-47	-0.2804728015077558
2216	48-49	-0.3078440104630218
2216	50-51	-0.36436590870771113
2216	52-53	-0.3421145993847077
2216	54-55	-0.20727353982131547
2216	56-57	-0.2315187828088341
2216	58-59	-0.2704809483408752
2216	60-61	-0.34762073554658457
2216	62-63	-0.36902004966538016
2216	64-65	-0.32792713475339497
2216	66-67	-0.3996736147174005
2216	68-69	-0.3400526533688364
2216	70-71	-0.38859033365408635
2216	72-73	-0.307287230724107
2216	74-75	-0.33077709250532195
2216	76-77	-0.33860673712558764
2216	78-79	-0.31715591608741533
2216	80-81	-0.2791763414482773
2216	82-83	-0.26476933573662365
2216	84-85	-0.25175651512645913
2216	86-87	-0.23143441690694289
2216	88-89	-0.35225067146395617
2216	90-91	-0.29121737432382133
2216	92-93	-0.3048331540459088
2216	94-95	-0.3342803126421785
2216	96-97	-0.31559784155870574
2216	98-99	-0.33713257890266135
2216	100	-0.4361213129606547
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	71.0
1	50.0
2	29.0
3	37.0
4	25.0
5	45.0
6	146.0
7	730.0
8	1976.0
9	2207.0
10	2085.0
11	2285.0
12	2634.0
13	3392.0
14	4975.0
15	5738.0
16	6115.0
17	6545.0
18	7140.0
19	8294.0
20	9479.0
21	10680.0
22	12206.0
23	14610.0
24	18331.0
25	23950.0
26	27203.0
27	25194.0
28	25428.0
29	32650.0
30	37723.0
31	39312.0
32	49713.0
33	67636.0
34	105718.0
35	166760.0
36	312587.0
37	708413.0
38	883483.0
39	315487.0
40	520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.301872877798615	20.78643315052892	13.183177912165439	23.728516059507022
2	15.075394310612591	21.2886593031194	40.311953479643265	23.323992906624742
3	17.607376125915547	27.41819613480286	30.610453364920566	24.36397437436103
4	12.088839750304368	18.144988969317055	40.448047336232015	29.318123944146564
5	12.707173679779904	39.24811610837834	35.29588760160639	12.748822610235369
6	28.371823875764047	39.67211810520862	18.87471347138386	13.081344547643479
7	25.59176807668406	33.798738341077616	23.660864232282314	16.94862934995601
8	25.280823051815045	35.02083841162314	21.29478626646853	18.403552270093286
9	26.49587643376286	15.566777022804896	21.298054726581277	36.639291816850964
10-11	24.132037170067715	26.836464553091915	28.89820456184285	20.133293714997524
12-13	24.84963137077851	25.167843075393737	28.151026810695996	21.83149874313176
14-15	22.559404318932526	26.282122003307716	25.849422985813614	25.309050691946144
16-17	21.81823590658932	28.89779647691294	26.86222562206498	22.421741994432757
18-19	21.56995236402635	28.771903699960173	28.26902570384306	21.389118232170425
20-21	23.157160929204096	27.859141177378522	27.266498016760053	21.717199876657332
22-23	23.25429836564367	27.729018111353803	26.839360535776514	22.177322987226013
24-25	22.687340960973927	27.997327889389577	27.339873091744167	21.97545805789233
26-27	22.41747501026277	28.19913257288266	26.959322036779387	22.424070380075182
28-29	22.123965108837275	28.572285782522634	27.05251607847537	22.251233030164723
30-31	22.47093295536585	28.096162760830794	27.11528546534725	22.317618818456104
32-33	22.496549564639118	28.39896569385837	26.53290021352836	22.571584527974153
34-35	22.79335441951298	28.282118940060784	27.08236223690299	21.84216440352325
36-37	22.72834298764861	27.859900706728858	27.019989728282294	22.391766577340235
38-39	22.559198632607895	27.838870371459667	27.31644199242929	22.28548900350314
40-41	22.59897782290866	27.534213249540855	27.48809066615257	22.378718261397914
42-43	22.429111444415845	27.710283758240195	27.154454100911995	22.706150696431965
44-45	22.316912832215525	28.145349972992094	27.29165529648255	22.246081898309832
46-47	22.38618536077114	27.92689107877944	26.935523443029812	22.751400117419603
48-49	22.65938407412357	27.70472855034038	27.063046997817874	22.57284037771818
50-51	22.66413732444357	27.814586088855815	26.775933706613646	22.745342880086973
52-53	22.393506106642835	27.913441279246122	26.914438309917394	22.77861430419365
54-55	22.39785320679106	27.89787157056518	26.824849042975313	22.879426179668446
56-57	22.87746372346171	27.684793713109613	26.700596639718576	22.737145923710102
58-59	22.330311860294806	27.69613715194384	27.296706906686424	22.676844081074936
60-61	22.364108385460913	27.97929769409857	27.030339984941556	22.626253935498966
62-63	22.62206543992663	27.64767783085909	26.716443364114905	23.013813365099374
64-65	22.05935113264261	27.74891935356072	26.8942131217394	23.29751639205727
66-67	22.59084845738499	27.4901485015095	26.813130570294426	23.105872470811086
68-69	22.26135449468781	27.508009391065226	26.969504204197005	23.261131910049958
70-71	22.19475491121302	27.384818387567954	27.049123182897944	23.37130351832108
72-73	22.599081782439125	27.342145968400544	27.242285005195388	22.816487243964943
74-75	22.496660865513864	27.761109264302164	26.945348719566265	22.796881150617708
76-77	22.330494777882087	27.407944475155173	27.08505957191607	23.17650117504667
78-79	22.302848702157394	27.58433940727039	27.0056651650587	23.107146725513513
80-81	22.250993827708417	27.656161629765215	27.233242835411115	22.85960170711526
82-83	22.645932366742322	27.00157727955665	27.880541648274143	22.47194870542689
84-85	22.773506847968786	27.56309773817837	26.925527351152382	22.737868062700457
86-87	22.164479905980002	27.751014996339247	27.197124038180657	22.88738105950009
88-89	22.682650763955493	27.123867345844012	27.1015938473456	23.09188804285489
90-91	22.49564341072354	27.072778377510765	27.31292850704799	23.118649704717697
92-93	22.79510783440227	27.183876189375027	27.229308785222162	22.791707191000537
94-95	21.997026358084437	27.16762307932455	27.685119751661453	23.15023081092956
96-97	22.230047387401786	27.13232256079003	27.394219995205148	23.243410056603036
98-99	22.004323774725044	27.589236300324664	27.12190137270921	23.284538552241077
100	22.371740529798757	26.981304600790878	26.952808730481774	23.69414613892859
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1336.0
1	982.0
2	698.0
3	782.0
4	709.0
5	610.0
6	574.0
7	551.0
8	572.5
9	584.0
10	498.0
11	394.5
12	394.5
13	407.0
14	403.0
15	481.0
16	562.0
17	655.0
18	833.0
19	1137.5
20	1577.5
21	2178.5
22	3089.5
23	4087.0
24	5448.0
25	7886.0
26	11830.5
27	15865.0
28	20368.0
29	26635.0
30	33956.0
31	41409.5
32	50619.0
33	58467.0
34	65590.0
35	73569.5
36	80836.5
37	91244.0
38	94728.5
39	92951.0
40	93210.5
41	92463.5
42	92760.5
43	95911.5
44	101091.5
45	109956.5
46	118075.0
47	122599.5
48	125427.5
49	121698.0
50	115464.0
51	114538.0
52	113390.0
53	108444.5
54	102058.5
55	91215.5
56	81363.0
57	68349.5
58	56141.0
59	51351.5
60	42709.0
61	33073.5
62	25837.5
63	19339.0
64	13666.0
65	8733.0
66	5970.0
67	4412.0
68	3329.0
69	2675.5
70	2264.0
71	1795.5
72	1400.0
73	1354.5
74	1156.0
75	885.0
76	686.0
77	498.0
78	400.0
79	306.5
80	189.5
81	115.5
82	89.5
83	62.0
84	50.0
85	52.5
86	42.5
87	26.5
88	19.0
89	23.0
90	18.5
91	13.0
92	12.0
93	9.5
94	7.5
95	7.0
96	8.0
97	7.5
98	3.5
99	4.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06282291078126817
2	0.009042691703364358
3	0.011184381843634864
4	0.008872716295406381
5	0.011898278557058365
6	0.012238229372974318
7	0.002651616364144436
8	0.005405217973063657
9	0.007342937623784591
10-11	0.006901001563093851
12-13	0.0055751933810216335
14-15	0.010079541691908014
16-17	0.009841576120766847
18-19	0.007903856470045913
20-21	0.006901001563093851
22-23	0.00261762128255284
24-25	0.0036374737303007
26-27	0.004912289289985525
28-29	0.005762166329775408
30-31	0.008685743346652605
32-33	0.010419492507823968
34-35	0.011524332659550817
36-37	0.017558459642058988
38-39	0.017983398161953927
40-41	0.01789841045797494
42-43	0.02321864072705961
44-45	0.027077082487705677
46-47	0.029456738199117354
48-49	0.030289617698111438
50-51	0.03115649227869712
52-53	0.029575720984687935
54-55	0.035014934039343186
56-57	0.0385504225248691
58-59	0.035303892232871746
60-61	0.03603478648709105
62-63	0.03180239882893743
64-65	0.03402907667318692
66-67	0.03601778894629525
68-69	0.03900935612635564
70-71	0.03865240776964389
72-73	0.03445401519308186
74-75	0.03720761680200109
76-77	0.03673168565971875
78-79	0.036969651230859914
80-81	0.04089608315468918
82-83	0.03725860942438848
84-85	0.0335021529085172
86-87	0.03232932259360716
88-89	0.030170634912540854
90-91	0.032006369318487
92-93	0.033383170122946615
94-95	0.03739458975075486
96-97	0.03219334226724078
98-99	0.030068649667766068
100	0.028045942313066147
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2941602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.73331911710597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.58176167094624	36.16543923807314
2	15.180280823469511	16.01013185902604
3	6.350730389420659	10.046852767560667
4	3.392945059297494	7.156850182349713
5	1.9180266962781718	5.057195692498266
6	1.1664964678727623	3.69079382935868
7	0.8049090883372757	2.971186947088388
8	0.5511900079900777	2.3252862868400763
9	0.409425794183656	1.9431342953515156
>10	1.614973224119289	13.352314046673039
>50	0.025057952855922862	0.8548327206615849
>100	0.004086104246533672	0.3154368112560323
>500	0.0	0.0
>1k	1.167209824308582E-4	0.11054532326282662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.6997540795797663E-5	0.0
12-13	0.0	0.0	0.0	3.3995081591595326E-5	0.0
14-15	0.0	0.0	0.0	3.3995081591595326E-5	0.0
16-17	0.0	0.0	0.0	1.1898278557058363E-4	0.0
18-19	0.0	0.0	0.0	1.869729487537743E-4	0.0
20-21	0.0	0.0	0.0	2.5496311193696494E-4	0.0
22-23	0.0	0.0	0.0	7.648893358108949E-4	0.0
24-25	0.0	0.0	0.0	0.0019037245691293384	0.0
26-27	0.0	0.0	0.0	0.003790451597462879	3.3995081591595326E-5
28-29	0.0	0.0	0.0	0.009977556447133228	3.3995081591595326E-5
30-31	0.0	0.0	0.0	0.02437447350117385	3.3995081591595326E-5
32-33	0.0	0.0	0.0	0.05340627318039626	3.3995081591595326E-5
34-35	0.0	0.0	0.0	0.09974156938974069	3.3995081591595326E-5
36-37	0.0	0.0	0.0	0.16977143746842704	3.3995081591595326E-5
38-39	0.0	0.0	0.0	0.2625780102134823	3.3995081591595326E-5
40-41	0.0	0.0	0.0	0.36094277879876335	3.3995081591595326E-5
42-43	0.0	0.0	0.0	0.4576757834676479	3.3995081591595326E-5
44-45	0.0	0.0	0.0	0.5713723338507385	3.3995081591595326E-5
46-47	0.0	0.0	0.0	0.70448007582263	3.3995081591595326E-5
48-49	0.0	0.0	0.0	0.8450327406630809	3.3995081591595326E-5
50-51	0.0	0.0	0.0	0.9852454546876157	3.3995081591595326E-5
52-53	0.0	0.0	0.0	1.121157790890814	3.3995081591595326E-5
54-55	0.0	0.0	0.0	1.2673876343570611	3.3995081591595326E-5
56-57	0.0	0.0	0.0	1.4316518686076498	3.3995081591595326E-5
58-59	0.0	0.0	0.0	1.6109759240033152	3.3995081591595326E-5
60-61	0.0	0.0	0.0	1.7983738112769845	3.3995081591595326E-5
62-63	0.0	0.0	0.0	1.9856867108466747	3.3995081591595326E-5
64-65	0.0	0.0	0.0	2.176433113657116	3.3995081591595326E-5
66-67	0.0	0.0	0.0	2.386760683464316	3.3995081591595326E-5
68-69	0.0	0.0	0.0	2.618080216154327	3.3995081591595326E-5
70-71	0.0	0.0	0.0	2.859666263484999	3.3995081591595326E-5
72-73	0.0	0.0	0.0	3.1038189394758366	3.3995081591595326E-5
74-75	0.0	0.0	0.0	3.346628809743806	3.3995081591595326E-5
76-77	0.0	0.0	0.0	3.6135071977786257	3.3995081591595326E-5
78-79	0.0	0.0	0.0	3.8981650134858485	3.3995081591595326E-5
80-81	0.0	0.0	0.0	4.198494561806799	3.3995081591595326E-5
82-83	0.0	0.0	0.0	4.509532560829099	3.3995081591595326E-5
84-85	0.0	0.0	0.0	4.815726260724599	3.3995081591595326E-5
86-87	0.0	0.0	0.0	5.139053481742262	3.3995081591595326E-5
88	0.0	0.0	0.0	5.389410260123565	3.3995081591595326E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4540	0.0	38.836395	1
GTATCAA	7305	0.0	33.98421	1
CAACGCA	8075	0.0	28.284145	5
ATCAACG	8195	0.0	28.042013	3
GTACTAG	1700	0.0	27.381027	1
TATCAAC	9065	0.0	27.061049	2
AACGCAG	9290	0.0	25.141003	6
GCATCGT	1600	0.0	24.377695	8
GTACATG	12950	0.0	23.853882	1
TCAACGC	9765	0.0	23.581594	4
TACATGG	12605	0.0	23.524935	2
ACGCAGA	9660	0.0	23.301985	7
CGCAGAG	9635	0.0	23.167356	8
ACATGGG	12370	0.0	22.946505	3
CTATTGA	2215	0.0	22.276663	9
GCTATTG	2420	0.0	21.554708	8
CATCGTC	1965	0.0	20.566977	9
GGGCTAT	2670	0.0	20.416847	6
GGCTATT	2500	0.0	20.30104	7
GCAGAGT	10570	0.0	20.139921	9
>>END_MODULE