FastQCFastQC Report
Mon 16 Jan 2017
ERR1146506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941602
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.44472365738125014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117250.3985923316614552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65080.2212399909981024No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT31720.10783239880854038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80500.057.596251
GTATCAA146600.042.832721
ATCAACG184350.033.5649723
CAACGCA185700.033.295645
TATCAAC189150.033.0961072
AACGCAG201100.031.2932036
TCAACGC203350.030.5444374
ACGCAGA213750.028.9565247
CGCAGAG215050.028.7377688
GCAGAGT230450.026.491059
GTGGTAT25800.025.5424271
GTACATG117400.023.6558041
ACATGGG111350.023.5200633
TACATGG114950.023.3807682
GAGTACT159200.023.30643712-13
CAGAGTA225650.023.20152910-11
AGAGTAC217200.022.07024210-11
CTGTGCG18800.021.7484449
AGTACTT164500.021.15562812-13
GTACTTT175000.021.02763714-15
Produced by FastQC (version 0.11.5)