##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2941602
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53979260280622	34.0	31.0	34.0	31.0	34.0
2	32.56869998048683	34.0	31.0	34.0	31.0	34.0
3	32.69705453015058	34.0	31.0	34.0	31.0	34.0
4	36.18677203782157	37.0	37.0	37.0	35.0	37.0
5	36.213176697595394	37.0	37.0	37.0	35.0	37.0
6	36.307891754221	37.0	37.0	37.0	35.0	37.0
7	36.29899762102419	37.0	37.0	37.0	35.0	37.0
8	36.314289968527355	37.0	37.0	37.0	35.0	37.0
9	38.126903979532244	39.0	39.0	39.0	37.0	39.0
10-11	38.101910625570696	39.0	38.5	39.0	37.0	39.0
12-13	38.082510312408	39.0	38.5	39.0	37.0	39.0
14-15	39.526657413205456	41.0	39.0	41.0	37.0	41.0
16-17	39.528851455771374	41.0	39.0	41.0	37.0	41.0
18-19	39.595021012359936	41.0	39.5	41.0	37.0	41.0
20-21	39.62992461250706	41.0	40.0	41.0	37.0	41.0
22-23	39.58558074817736	41.0	40.0	41.0	37.0	41.0
24-25	39.560827229516434	41.0	40.0	41.0	36.5	41.0
26-27	39.47889959280691	41.0	39.0	41.0	36.0	41.0
28-29	39.38805878565489	41.0	39.0	41.0	36.0	41.0
30-31	39.25919396981645	41.0	39.0	41.0	35.5	41.0
32-33	39.105464301424874	41.0	39.0	41.0	35.0	41.0
34-35	39.002177554951345	41.0	39.0	41.0	35.0	41.0
36-37	38.902221476596765	41.0	39.0	41.0	35.0	41.0
38-39	38.783068205692004	41.0	38.0	41.0	35.0	41.0
40-41	38.67597706963757	40.5	38.0	41.0	35.0	41.0
42-43	38.55441932661182	40.0	38.0	41.0	35.0	41.0
44-45	38.361376386064464	40.0	38.0	41.0	34.5	41.0
46-47	38.064895590905905	40.0	38.0	41.0	33.5	41.0
48-49	37.78536576328137	40.0	37.5	41.0	33.0	41.0
50-51	37.604068463374716	40.0	37.0	41.0	33.0	41.0
52-53	37.460348816733195	40.0	37.0	41.0	33.0	41.0
54-55	37.31375301621361	40.0	36.0	41.0	33.0	41.0
56-57	37.09556238403428	40.0	36.0	41.0	32.5	41.0
58-59	36.87144963866628	39.0	35.0	41.0	32.0	41.0
60-61	36.684613860066726	39.0	35.0	41.0	32.0	41.0
62-63	36.429767861185844	39.0	35.0	41.0	31.5	41.0
64-65	36.14048365482482	38.0	35.0	41.0	31.0	41.0
66-67	35.892905294462	37.0	35.0	40.0	31.0	41.0
68-69	35.59231568376687	37.0	35.0	40.0	31.0	41.0
70-71	35.329874843707614	36.0	35.0	39.0	31.0	41.0
72-73	35.023779899524136	36.0	35.0	39.0	30.5	41.0
74-75	34.70376532923217	35.0	35.0	39.0	30.0	40.5
76-77	34.40989824592178	35.0	35.0	37.0	30.0	39.5
78-79	34.13445819658811	35.0	35.0	37.0	30.0	39.0
80-81	33.89395336282747	35.0	35.0	36.5	30.0	39.0
82-83	33.64765610711442	35.0	34.0	36.0	29.0	37.5
84-85	33.44709005501085	35.0	34.0	36.0	29.0	37.0
86-87	33.27178557806256	35.0	34.0	35.5	29.0	37.0
88-89	33.13086032712788	35.0	34.0	35.0	29.0	36.0
90-91	33.04594078328748	35.0	34.0	35.0	29.0	36.0
92-93	32.96704159842154	35.0	34.0	35.0	29.0	36.0
94-95	32.899983240424774	35.0	34.0	35.0	29.0	36.0
96-97	32.8562852826453	35.0	34.0	35.0	29.0	35.5
98-99	32.80661710863672	35.0	34.0	35.0	29.0	35.0
100	32.264562983027616	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24826585355076958
1101	2	0.17625397866475367
1101	3	0.2403986447156896
1101	4	0.19524873030700718
1101	5	0.18936536809120952
1101	6	0.15379098207569797
1101	7	0.19366275265543464
1101	8	0.17560040037199798
1101	9	0.16178097456020168
1101	10-11	0.2027005345444195
1101	12-13	0.2564497151598033
1101	14-15	0.37327946813491764
1101	16-17	0.36104160374032546
1101	18-19	0.28944845293703025
1101	20-21	0.31465137663806075
1101	22-23	0.3484845820458773
1101	24-25	0.3031045031476296
1101	26-27	0.36245863835177516
1101	28-29	0.35926274712508643
1101	30-31	0.3380547302652914
1101	32-33	0.3868858768683907
1101	34-35	0.37562204195884874
1101	36-37	0.4259318678921957
1101	38-39	0.45500519169388554
1101	40-41	0.4592250262360338
1101	42-43	0.39432062301173687
1101	44-45	0.39845560048031103
1101	46-47	0.4344864612103052
1101	48-49	0.4737871246217722
1101	50-51	0.5013754185258747
1101	52-53	0.4887678287487134
1101	54-55	0.38056752626596335
1101	56-57	0.47310517651443007
1101	58-59	0.4772048824971762
1101	60-61	0.3926937635135914
1101	62-63	0.36715598594140175
1101	64-65	0.3537483667057941
1101	66-67	0.3179843460337892
1101	68-69	0.3632287241817309
1101	70-71	0.3325410728902938
1101	72-73	0.30135982700612374
1101	74-75	0.27629748471517956
1101	76-77	0.39834380019899385
1101	78-79	0.3595132997075865
1101	80-81	0.35682267531804257
1101	82-83	0.37172836931002706
1101	84-85	0.42408370390735683
1101	86-87	0.3527589563391942
1101	88-89	0.3124306702238968
1101	90-91	0.3294331241053712
1101	92-93	0.31446758633906313
1101	94-95	0.345715410578336
1101	96-97	0.312090281226979
1101	98-99	0.2947084848570043
1101	100	0.29964656790708233
1102	1	0.2200986775894762
1102	2	0.20528377240043483
1102	3	0.251647689788264
1102	4	0.17618837889371264
1102	5	0.15247652164354264
1102	6	0.17917613613732186
1102	7	0.16551785833406996
1102	8	0.14965820531215712
1102	9	0.21187646730275134
1102	10-11	0.2165262921201716
1102	12-13	0.20811288042590803
1102	14-15	0.30350928733532356
1102	16-17	0.30262232693868896
1102	18-19	0.24591649469919474
1102	20-21	0.2924031591692611
1102	22-23	0.31700858255516806
1102	24-25	0.2795607674715441
1102	26-27	0.31221354031256254
1102	28-29	0.380486009157174
1102	30-31	0.3629258150628445
1102	32-33	0.31440529372603976
1102	34-35	0.3651130656410544
1102	36-37	0.3876297288547548
1102	38-39	0.4110482907516868
1102	40-41	0.3829899867657005
1102	42-43	0.31508870297098923
1102	44-45	0.38340116961705917
1102	46-47	0.41508074437812326
1102	48-49	0.4261744431855661
1102	50-51	0.4479663276167827
1102	52-53	0.4621205789396967
1102	54-55	0.36235069407854326
1102	56-57	0.4945098747573624
1102	58-59	0.5341615287920689
1102	60-61	0.4607928213185346
1102	62-63	0.4388280638634754
1102	64-65	0.4992442923487843
1102	66-67	0.4785426602685803
1102	68-69	0.444187471316944
1102	70-71	0.38498887019103734
1102	72-73	0.42748027518540255
1102	74-75	0.47079551119849583
1102	76-77	0.3671638282101881
1102	78-79	0.30137572649648803
1102	80-81	0.3530093317988232
1102	82-83	0.4275820744284431
1102	84-85	0.36103302909198476
1102	86-87	0.29603308417214436
1102	88-89	0.3572390481205119
1102	90-91	0.28330694636154163
1102	92-93	0.3127853225244479
1102	94-95	0.3580992277415618
1102	96-97	0.2779993721360725
1102	98-99	0.2919169136830746
1102	100	0.27357386609908474
1103	1	0.16164539308261539
1103	2	0.18407268711064262
1103	3	0.21366459028562446
1103	4	0.103529649694174
1103	5	0.13769225534704788
1103	6	0.11650837427054483
1103	7	0.12372083810711132
1103	8	0.12140270427680377
1103	9	0.1693845158437739
1103	10-11	0.15716553245530918
1103	12-13	0.18925905798759857
1103	14-15	0.28183599364329126
1103	16-17	0.2411335022386254
1103	18-19	0.23629162519662827
1103	20-21	0.26937082889650554
1103	22-23	0.2359648174263853
1103	24-25	0.2379456451348716
1103	26-27	0.25303079382923244
1103	28-29	0.3401447835344342
1103	30-31	0.31495717962577885
1103	32-33	0.3278228259633664
1103	34-35	0.2818058821527316
1103	36-37	0.2814037349160614
1103	38-39	0.3048472236941322
1103	40-41	0.2625394123467757
1103	42-43	0.2686930148826008
1103	44-45	0.29341458912762164
1103	46-47	0.2911351190781346
1103	48-49	0.27841522951968045
1103	50-51	0.2513418661525151
1103	52-53	0.1996291900440994
1103	54-55	0.1557710189898316
1103	56-57	0.21405731913696258
1103	58-59	0.21929843887745903
1103	60-61	0.20812316313836732
1103	62-63	0.1880008726863096
1103	64-65	0.13395354635809298
1103	66-67	0.12220285875130088
1103	68-69	0.19169737300791923
1103	70-71	0.2645084410659635
1103	72-73	0.28673598225418573
1103	74-75	0.2597495361020705
1103	76-77	0.17585238938313097
1103	78-79	0.18831937145827737
1103	80-81	0.1685263836559443
1103	82-83	0.20802790374757762
1103	84-85	0.1783806420391727
1103	86-87	0.14416377670924874
1103	88-89	0.1379379590208316
1103	90-91	0.09963180182160869
1103	92-93	0.1285619664468598
1103	94-95	0.1523771021095115
1103	96-97	0.07774277992920275
1103	98-99	0.05535203501835895
1103	100	0.0870096492736323
1104	1	0.16799685060657765
1104	2	0.1819067154730476
1104	3	0.10688969782196267
1104	4	0.09099402892720576
1104	5	0.07240687713348848
1104	6	0.07504821806974604
1104	7	0.08814340921450281
1104	8	0.07170225623702464
1104	9	0.15850393014203235
1104	10-11	0.1094821355173039
1104	12-13	0.07840119980974691
1104	14-15	0.15521203084453106
1104	16-17	0.1296308321207107
1104	18-19	0.16092808134322212
1104	20-21	0.19330016214124868
1104	22-23	0.14994040724024416
1104	24-25	0.16261105990153624
1104	26-27	0.19136411140730303
1104	28-29	0.20399027258695668
1104	30-31	0.1744096127290362
1104	32-33	0.10361054370056877
1104	34-35	0.1722839816470909
1104	36-37	0.22479795413639891
1104	38-39	0.22153587006437903
1104	40-41	0.21672191582812417
1104	42-43	0.1812715676359602
1104	44-45	0.11786833302451072
1104	46-47	0.18560564699178173
1104	48-49	0.10429633381371417
1104	50-51	0.12204332514771465
1104	52-53	0.14444377964985478
1104	54-55	0.08895615963618297
1104	56-57	0.13404974462710584
1104	58-59	0.10031412619466096
1104	60-61	0.09718165782003041
1104	62-63	0.056989545805002706
1104	64-65	0.030794187537885875
1104	66-67	0.06071665177531571
1104	68-69	0.08130142146294617
1104	70-71	0.011569840130789544
1104	72-73	-0.003836342598418696
1104	74-75	0.024873990931084222
1104	76-77	0.025953351781261347
1104	78-79	0.06602818407896649
1104	80-81	0.08661000336894631
1104	82-83	0.04184776408847313
1104	84-85	-0.020380541480335523
1104	86-87	0.07231181503900785
1104	88-89	0.0815434430245503
1104	90-91	0.017700609366876563
1104	92-93	0.007560236518813213
1104	94-95	0.003234883064379801
1104	96-97	-0.043758968838595536
1104	98-99	-0.08712554069112599
1104	100	0.0336868440075051
1105	1	0.13761157249599165
1105	2	0.08718024472243968
1105	3	0.11855383329015723
1105	4	0.07626436673565706
1105	5	0.02782767757980764
1105	6	0.003327605235597275
1105	7	0.08616813004817203
1105	8	0.04359759925154805
1105	9	0.09456735797236604
1105	10-11	0.08036701699813165
1105	12-13	0.12139751246053976
1105	14-15	0.15235680506582128
1105	16-17	0.14224192719659356
1105	18-19	0.13099639706632615
1105	20-21	0.13958988202087852
1105	22-23	0.14314321919512452
1105	24-25	0.13435778782707075
1105	26-27	0.08826815945229072
1105	28-29	0.11585547125536522
1105	30-31	0.13913874220661882
1105	32-33	0.11289647453105545
1105	34-35	0.027672359184386153
1105	36-37	0.043393291533213585
1105	38-39	0.11111794627347393
1105	40-41	0.11166158112878577
1105	42-43	0.08325958350889096
1105	44-45	0.07892306793151249
1105	46-47	0.01748299638143891
1105	48-49	0.04853213868155848
1105	50-51	0.08559642801283474
1105	52-53	0.09334640098999358
1105	54-55	0.0073205292079592255
1105	56-57	0.009457028667284817
1105	58-59	0.038055905691763314
1105	60-61	0.09656927890350175
1105	62-63	0.07022082889986336
1105	64-65	0.061715509075320085
1105	66-67	0.015431303540204055
1105	68-69	-0.0892644491030623
1105	70-71	0.0028160784437787356
1105	72-73	-0.002196431087057249
1105	74-75	-0.005881402313526962
1105	76-77	-0.04882643153006683
1105	78-79	-0.08086902343443114
1105	80-81	-0.030061498080513616
1105	82-83	-5.518483863369283E-4
1105	84-85	-0.030465543257662375
1105	86-87	0.028999142936079636
1105	88-89	0.052026972961627393
1105	90-91	0.008104312116365975
1105	92-93	-0.047457493170497855
1105	94-95	-0.06785004461728761
1105	96-97	-0.09792169261478278
1105	98-99	-0.0223388659982362
1105	100	-0.05626517783570506
1106	1	0.16348856235260456
1106	2	0.18292537961950472
1106	3	0.20766104263260843
1106	4	0.14694321091614881
1106	5	0.13358361877176606
1106	6	0.11399337289117994
1106	7	0.11629067706463303
1106	8	0.12329243493229569
1106	9	0.17792884215794658
1106	10-11	0.16274412944716943
1106	12-13	0.15316234387326233
1106	14-15	0.19434902271731147
1106	16-17	0.21068357320416453
1106	18-19	0.20765968109027
1106	20-21	0.19450402192152438
1106	22-23	0.22989461084612373
1106	24-25	0.27301810239014657
1106	26-27	0.27137685352517593
1106	28-29	0.28514845352032836
1106	30-31	0.314689045963398
1106	32-33	0.2569434773733832
1106	34-35	0.2874459264422242
1106	36-37	0.29693632601347275
1106	38-39	0.3477903339197894
1106	40-41	0.3456218384820673
1106	42-43	0.3220398797285071
1106	44-45	0.35394145679467215
1106	46-47	0.4097468470547909
1106	48-49	0.46762465963578137
1106	50-51	0.46175435883439064
1106	52-53	0.4387717739887833
1106	54-55	0.4771371400269615
1106	56-57	0.46504406474577564
1106	58-59	0.4404549712650834
1106	60-61	0.4455422268425835
1106	62-63	0.4291110496570312
1106	64-65	0.44938986274083703
1106	66-67	0.41388425529327577
1106	68-69	0.4369790693902402
1106	70-71	0.39662459362253344
1106	72-73	0.34486793447446473
1106	74-75	0.3384586872243531
1106	76-77	0.361240789980819
1106	78-79	0.3614494262708945
1106	80-81	0.38635181091594717
1106	82-83	0.3687388636486091
1106	84-85	0.37836428938098265
1106	86-87	0.3555896660878872
1106	88-89	0.3610261647597426
1106	90-91	0.37704132231423415
1106	92-93	0.29321753702356546
1106	94-95	0.3179724824211547
1106	96-97	0.27478310483530066
1106	98-99	0.28839807336846235
1106	100	0.30264297773861415
1107	1	1.1368945932588304E-4
1107	2	0.020267713190825987
1107	3	0.06414943535452977
1107	4	0.03737396749865951
1107	5	0.030871648022539944
1107	6	0.0015455677809512736
1107	7	-0.03704114180557383
1107	8	0.04947317528841211
1107	9	0.0079768722741278
1107	10-11	0.013990857125065759
1107	12-13	-0.003916689466564094
1107	14-15	0.049485757102118555
1107	16-17	0.06554824510654811
1107	18-19	0.0867557629578144
1107	20-21	0.07074155464474785
1107	22-23	0.08706775725143956
1107	24-25	0.08764186648006245
1107	26-27	0.07859218854604677
1107	28-29	0.1435245163489114
1107	30-31	0.10585539891985007
1107	32-33	0.12451124959812887
1107	34-35	0.1162066629040055
1107	36-37	0.1646528401520655
1107	38-39	0.20359559559738472
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2216	30-31	-0.4732682024106438
2216	32-33	-0.5107526059413772
2216	34-35	-0.5077868150160683
2216	36-37	-0.5274745266629353
2216	38-39	-0.43901933627156353
2216	40-41	-0.44345962458623234
2216	42-43	-0.4933224638626257
2216	44-45	-0.4626786410236292
2216	46-47	-0.463960610444218
2216	48-49	-0.5547622270825272
2216	50-51	-0.5316339320651551
2216	52-53	-0.4683206853366144
2216	54-55	-0.5270961185115794
2216	56-57	-0.45453378745781947
2216	58-59	-0.45660739620345225
2216	60-61	-0.4347845299257074
2216	62-63	-0.49599979843365105
2216	64-65	-0.4363886513887465
2216	66-67	-0.3583123632865082
2216	68-69	-0.38444924225577637
2216	70-71	-0.4373846205261742
2216	72-73	-0.4094958591551716
2216	74-75	-0.4102350130936472
2216	76-77	-0.31060917135675936
2216	78-79	-0.3065458757308619
2216	80-81	-0.29554393653383215
2216	82-83	-0.3261807438131541
2216	84-85	-0.32329740414380836
2216	86-87	-0.2978423552531453
2216	88-89	-0.33361596668617466
2216	90-91	-0.3752897687567227
2216	92-93	-0.37728821331997153
2216	94-95	-0.32154502003378127
2216	96-97	-0.3409995838871325
2216	98-99	-0.4241993140321725
2216	100	-0.43622145393958434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	33.0
8	70.0
9	122.0
10	224.0
11	241.0
12	175.0
13	241.0
14	324.0
15	685.0
16	1262.0
17	2523.0
18	4725.0
19	7610.0
20	10834.0
21	16177.0
22	23601.0
23	27820.0
24	25982.0
25	20824.0
26	16719.0
27	17294.0
28	19899.0
29	23141.0
30	27057.0
31	34750.0
32	45683.0
33	60748.0
34	92956.0
35	153551.0
36	271311.0
37	690448.0
38	954481.0
39	388977.0
40	1112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73152884117525	20.380242718100032	13.607022689930126	23.281205750794594
2	15.264883683502624	21.339554482177142	40.15240521713627	23.243156617183967
3	17.445480959767636	27.77582305061859	31.28358943658644	23.495106553027327
4	11.974597515231496	18.406466952361335	39.880344111813905	29.73859142059327
5	12.129932714266038	38.85007201290961	35.3705986678887	13.64939660493565
6	27.87600405378162	39.83231405180641	18.363249974247402	13.928431920164572
7	24.480172531896745	34.557609954579156	23.665718090250852	17.296499423273247
8	25.54373433251677	34.141192452276	21.105948391386733	19.20912482382049
9	26.703476541014048	14.340485218598573	21.509164054144648	37.44687418624274
10-11	24.134570210381963	26.33918524667851	28.764258387096554	20.761986155842973
12-13	25.592330301651955	24.778318072941207	28.562055641789748	21.06729598361709
14-15	22.780312017110408	25.359417310604442	27.38773419853097	24.47253647375418
16-17	20.854962703995987	28.176024492776385	28.265635527851828	22.7033772753758
18-19	20.61986971724931	26.735805863607652	31.592105254211823	21.05221916493122
20-21	22.058694548072786	25.85633610529229	31.112519640658387	20.97244970597654
22-23	22.04404760690647	25.882957856125426	30.728840525022182	21.34415401194592
24-25	21.609160586646322	26.103344368136817	31.18764877097582	21.099846274241045
26-27	21.42364942640099	26.36225090953841	30.73218266781162	21.48191699624898
28-29	21.071233160315277	26.65217455599426	30.973240587904442	21.30335169578602
30-31	21.46988033723144	26.145312075061188	30.966055887952137	21.418751699755234
32-33	21.56776623024306	26.521037639539006	30.304504989017733	21.606691141200198
34-35	21.78421878811212	26.43570137350239	30.74473048808161	21.03534935030388
36-37	21.790880177890703	26.088959289416252	30.540476271012718	21.579684261680324
38-39	21.731261999281	25.984244874995007	30.780370769815228	21.504122355908766
40-41	21.68870623482632	25.944110372521028	30.737425360747533	21.62975803190512
42-43	21.81455266710849	26.061260521327878	30.168366742481815	21.955820069081817
44-45	21.73478864802865	26.57212809688379	30.046495325990808	21.646587929096754
46-47	22.095376633831258	26.42249150587072	29.195821104263153	22.286310756034872
48-49	22.491251729724297	26.548491007303383	28.87225800135063	22.087999261621682
50-51	22.411428961889353	26.783331880032478	28.32109416046719	22.484144997610976
52-53	22.087715418588612	27.054383722703136	28.37908470291115	22.4788161557971
54-55	22.086731323814824	27.039635677174783	28.175322880627913	22.69831011838248
56-57	22.685305428110645	26.79818573444983	27.896542533662227	22.619966303777296
58-59	22.013430758773108	26.91273236407633	28.574961716371217	22.498875160779342
60-61	22.127958084782993	27.251945143337352	28.222655552824577	22.39744121905508
62-63	22.30835900977521	26.955751078206585	27.921866333829044	22.814023578189165
64-65	21.745365115849367	27.031377673174013	28.143678476462707	23.079578734513913
66-67	22.194345047016387	26.80723562281434	28.05212147853058	22.946297851638693
68-69	21.983923141123814	26.70730359902023	28.27635267100573	23.03242058885023
70-71	21.95797800823034	26.555597294628182	28.256245421214288	23.23017927592719
72-73	22.36523362700354	26.4891839964045	28.3696501540141	22.775932222577865
74-75	22.302749906042852	26.99787064459109	27.885886811849936	22.813492637516124
76-77	22.169881317617353	26.66851152632163	27.902012115220554	23.259595040840466
78-79	22.04670619191047	26.900458257127124	27.856781049639988	23.19605450132242
80-81	21.916477753004784	26.99267864303077	28.072091391095217	23.018752212869224
82-83	22.381017376454377	26.27806916348628	28.672857977923886	22.66805548213546
84-85	22.57777915269184	26.808676780443825	27.640673538668437	22.972870528195898
86-87	21.824985781552293	27.013860819329338	27.996947920537153	23.16420547858122
88-89	22.229198785012894	26.474089737030422	27.90338234869178	23.393329129264906
90-91	22.055057074342486	26.450383158564616	28.048067005665622	23.446492761427276
92-93	22.379506133052672	26.539348287089826	27.86377966835758	23.217365911499925
94-95	21.529207814178772	26.504796791815615	28.374866548035143	23.59112884597047
96-97	21.780595756340496	26.458204470135776	28.119160786765384	23.642038986758347
98-99	21.605618595935734	26.804135451961507	27.878979694308075	23.71126625779468
100	21.90451325324715	26.228242567517263	27.72094326146382	24.14630091777177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	452.0
1	308.5
2	424.0
3	621.0
4	358.0
5	248.0
6	599.0
7	1511.5
8	2960.0
9	3605.0
10	2526.5
11	1526.0
12	1579.5
13	1859.0
14	2136.5
15	2486.5
16	2954.5
17	3471.5
18	4174.0
19	4951.5
20	5695.0
21	6528.5
22	7588.5
23	8775.5
24	10259.0
25	12489.0
26	16162.5
27	20102.0
28	24210.5
29	30040.0
30	36599.0
31	42992.0
32	51273.5
33	58151.5
34	64038.5
35	71307.0
36	78152.5
37	87921.0
38	91259.5
39	89580.0
40	90237.0
41	89109.0
42	89112.5
43	92595.5
44	97908.0
45	106738.0
46	114907.5
47	119389.5
48	122335.5
49	118994.0
50	113110.0
51	111645.0
52	110116.5
53	105418.5
54	99440.5
55	89148.0
56	79788.5
57	67872.0
58	55938.0
59	50998.5
60	42451.5
61	32618.5
62	25188.5
63	18778.5
64	13167.5
65	8251.0
66	5409.5
67	3968.0
68	3013.0
69	2399.5
70	2022.5
71	1612.5
72	1250.0
73	1172.5
74	1028.5
75	807.0
76	626.5
77	439.0
78	327.0
79	215.0
80	144.5
81	86.0
82	53.0
83	39.0
84	28.5
85	19.5
86	12.0
87	7.0
88	5.5
89	4.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005609188462613229
2	0.08821723673018987
3	5.099262238739299E-4
4	0.0
5	0.03933230940147579
6	0.005133257320330894
7	3.3995081591595326E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6997540795797663E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.079409790991439E-4
24-25	0.0
26-27	0.0
28-29	5.099262238739299E-5
30-31	6.799016318319065E-5
32-33	0.0013937983452554084
34-35	0.0011728303149100387
36-37	6.968991726277042E-4
38-39	6.62904091036109E-4
40-41	0.0012408204780932296
42-43	5.949139278529182E-4
44-45	5.439213054655252E-4
46-47	3.909434383033463E-4
48-49	6.968991726277042E-4
50-51	7.648893358108949E-4
52-53	0.001104840151726848
54-55	0.0011898278557058363
56-57	8.838721213814785E-4
58-59	0.0023966532522074705
60-61	0.0015297786716217897
62-63	9.348647437688715E-4
64-65	0.004470353229294785
66-67	0.004334372902928404
68-69	0.0036374737303007
70-71	0.0015977688348049804
72-73	0.005116259779535097
74-75	0.0034505007815469257
76-77	0.003246530291997354
78-79	7.478917950150973E-4
80-81	8.498770397898832E-5
82-83	2.2096803034536963E-4
84-85	1.359803263663813E-4
86-87	3.3995081591595326E-5
88-89	2.379655711411673E-4
90-91	0.0
92-93	0.0
94-95	1.6997540795797663E-5
96-97	4.589336014865369E-4
98-99	4.4193606069073925E-4
100	7.138967134235019E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2941602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.58548961673807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.2842695176801	32.20453688404315
2	16.298264039523687	15.837182765262673
3	6.822652744977972	9.944457722992105
4	3.532896350355848	6.865899957889036
5	2.062102293251118	5.009412477870198
6	1.2874487604804363	3.753079703064273
7	0.8896357674336467	3.025637253892838
8	0.6007124747769719	2.334872776473728
9	0.42095816935703345	1.8407212889739493
>10	1.7449961692662184	13.501691593024132
>50	0.039374019613193485	1.2615751123218326
>100	0.013970098462183689	1.3215163204917264
>500	0.0014834153572440866	0.5291920480989138
>1k	8.653256250590506E-4	0.6894331540264932
>5k	1.2361794643700724E-4	0.390872982524995
>10k+	2.4723589287401447E-4	1.4899179590500233
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13082	0.44472365738125014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11725	0.3985923316614552	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6508	0.2212399909981024	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	3172	0.10783239880854038	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.6997540795797663E-5	0.0
12-13	0.0	0.0	0.0	3.3995081591595326E-5	0.0
14-15	0.0	0.0	0.0	5.099262238739299E-5	0.0
16-17	0.0	0.0	0.0	1.1898278557058363E-4	0.0
18-19	0.0	0.0	0.0	1.5297786716217897E-4	0.0
20-21	0.0	0.0	0.0	2.2096803034536963E-4	0.0
22-23	0.0	0.0	0.0	7.308942542192995E-4	0.0
24-25	0.0	0.0	0.0	0.001869729487537743	0.0
26-27	0.0	0.0	0.0	0.003756456515871284	0.0
28-29	0.0	0.0	0.0	0.009977556447133228	0.0
30-31	0.0	0.0	0.0	0.024374473501173853	0.0
32-33	0.0	0.0	0.0	0.05367823383312902	0.0
34-35	0.0	0.0	0.0	0.10023449807281883	0.0
36-37	0.0	0.0	0.0	0.1704343415594632	0.0
38-39	0.0	0.0	0.0	0.2634278872532722	0.0
40-41	0.0	0.0	0.0	0.36211560911367346	0.0
42-43	0.0	0.0	0.0	0.4590525842721075	0.0
44-45	0.0	0.0	0.0	0.5728511198999728	0.0
46-47	0.0	0.0	0.0	0.7063668028509635	0.0
48-49	0.0	0.0	0.0	0.8475823717824504	0.0
50-51	0.0	0.0	0.0	0.9882880144900636	0.0
52-53	0.0	0.0	0.0	1.1246932793763398	1.6997540795797663E-5
54-55	0.0	0.0	0.0	1.2715520318520317	3.3995081591595326E-5
56-57	0.0	0.0	0.0	1.4358332636434161	3.3995081591595326E-5
58-59	0.0	0.0	0.0	1.6160071960788713	3.3995081591595326E-5
60-61	0.0	0.0	0.0	1.8037620317092524	3.3995081591595326E-5
62-63	0.0	0.0	0.0	1.9909049558709846	3.3995081591595326E-5
64-65	0.0	0.0	0.0	2.1821272898237085	3.3995081591595326E-5
66-67	0.0	0.0	0.0	2.393355729293086	3.3995081591595326E-5
68-69	0.0	0.0	0.0	2.6256611193492523	3.3995081591595326E-5
70-71	0.0	0.0	0.0	2.8682670191276727	3.3995081591595326E-5
72-73	0.0	0.0	0.0	3.11432341968764	3.3995081591595326E-5
74-75	0.0	0.0	0.0	3.358935029279964	3.3995081591595326E-5
76-77	0.0	0.0	0.0	3.6278531222102783	3.3995081591595326E-5
78-79	0.0	0.0	0.0	3.914890593628914	3.3995081591595326E-5
80-81	0.0	0.0	0.0	4.217310839467746	3.3995081591595326E-5
82-83	0.0	0.0	0.0	4.5307454917422545	3.3995081591595326E-5
84-85	0.0	0.0	0.0	4.837755073595952	3.3995081591595326E-5
86-87	0.0	0.0	0.0	5.161881179031018	3.3995081591595326E-5
88	0.0	0.0	0.0	5.413546768053598	3.3995081591595326E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8050	0.0	57.59625	1
GTATCAA	14660	0.0	42.83272	1
ATCAACG	18435	0.0	33.564972	3
CAACGCA	18570	0.0	33.29564	5
TATCAAC	18915	0.0	33.096107	2
AACGCAG	20110	0.0	31.293203	6
TCAACGC	20335	0.0	30.544437	4
ACGCAGA	21375	0.0	28.956524	7
CGCAGAG	21505	0.0	28.737768	8
GCAGAGT	23045	0.0	26.49105	9
GTGGTAT	2580	0.0	25.542427	1
GTACATG	11740	0.0	23.655804	1
ACATGGG	11135	0.0	23.520063	3
TACATGG	11495	0.0	23.380768	2
GAGTACT	15920	0.0	23.306437	12-13
CAGAGTA	22565	0.0	23.201529	10-11
AGAGTAC	21720	0.0	22.070242	10-11
CTGTGCG	1880	0.0	21.748444	9
AGTACTT	16450	0.0	21.155628	12-13
GTACTTT	17500	0.0	21.027637	14-15
>>END_MODULE