FastQCFastQC Report
Mon 16 Jan 2017
ERR1146505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043580
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.040.6832431
GTATCAA69650.033.616981
CAACGCA75250.029.6645495
ATCAACG76800.029.2494263
TATCAAC85700.026.869452
AACGCAG87150.026.3676076
GTACATG137450.025.8257351
TACATGG135300.025.7720872
TCAACGC90500.024.8735394
TATTCGC2651.8189894E-1224.8266759
GTACTAG13600.024.545391
ACGCAGA90050.024.5265357
ACATGGG138650.024.2685473
CGCAGAG92300.024.082188
CATGGGG92200.022.9877384
CTGTGCG21300.022.0626059
CGTTAAC4050.022.0571331
GTGATCG10450.022.0351568
TGATCGC10400.021.6892389
GTATAAG17150.021.6577551
Produced by FastQC (version 0.11.5)