##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146505_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3043580
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43871559150737	31.0	31.0	34.0	28.0	34.0
2	31.485909028183915	33.0	31.0	34.0	28.0	34.0
3	31.668472982474587	33.0	31.0	34.0	28.0	34.0
4	35.11926415602679	37.0	35.0	37.0	32.0	37.0
5	35.19043921960323	37.0	35.0	37.0	32.0	37.0
6	35.1447019628201	37.0	35.0	37.0	32.0	37.0
7	35.12142936936108	37.0	35.0	37.0	32.0	37.0
8	35.10016066605773	37.0	35.0	37.0	32.0	37.0
9	36.66597165180478	39.0	37.0	39.0	32.0	39.0
10-11	36.6319893020719	39.0	37.0	39.0	32.0	39.0
12-13	36.58184227127265	39.0	37.0	39.0	32.0	39.0
14-15	37.98478978702712	40.0	38.0	41.0	32.0	41.0
16-17	38.0076418231162	40.0	38.0	41.0	32.0	41.0
18-19	38.04800958082258	40.0	38.0	41.0	32.0	41.0
20-21	38.155909652448756	40.0	38.5	41.0	33.0	41.0
22-23	38.197451356626075	40.0	38.5	41.0	33.0	41.0
24-25	38.26266945504965	40.5	38.0	41.0	33.0	41.0
26-27	38.30296969358453	40.0	38.0	41.0	33.0	41.0
28-29	38.273065271818055	40.0	38.0	41.0	33.0	41.0
30-31	38.25493234940432	40.0	38.0	41.0	33.0	41.0
32-33	38.26272005335822	40.0	38.0	41.0	33.0	41.0
34-35	38.21061184526117	40.0	38.0	41.0	33.0	41.0
36-37	38.11312894683235	40.0	38.0	41.0	33.0	41.0
38-39	38.065515609906754	40.0	38.0	41.0	32.0	41.0
40-41	37.99165242904738	40.0	38.0	41.0	32.0	41.0
42-43	37.8525992416825	40.0	38.0	41.0	32.0	41.0
44-45	37.69274702817077	40.0	37.0	41.0	32.0	41.0
46-47	37.58049632340862	40.0	37.0	41.0	31.0	41.0
48-49	37.458413611602126	40.0	37.0	41.0	31.0	41.0
50-51	37.31915490967873	40.0	36.0	41.0	31.0	41.0
52-53	37.181388036457065	40.0	36.0	41.0	31.0	41.0
54-55	36.995652488188256	39.5	35.0	41.0	30.0	41.0
56-57	36.847811623154314	39.0	35.0	41.0	30.0	41.0
58-59	36.61376421845327	39.0	35.0	41.0	30.0	41.0
60-61	36.421084052333114	38.5	35.0	40.5	30.0	41.0
62-63	36.174447032770615	38.0	35.0	40.0	30.0	41.0
64-65	35.9186814540771	37.0	35.0	40.0	30.0	41.0
66-67	35.65903853356902	37.0	35.0	40.0	30.0	41.0
68-69	35.36702025246585	36.0	35.0	39.5	29.0	41.0
70-71	35.10985402059417	36.0	34.0	39.0	29.0	41.0
72-73	34.808650667963384	35.0	34.0	39.0	29.0	41.0
74-75	34.50393040432648	35.0	34.0	37.5	29.0	40.0
76-77	34.212688840115916	35.0	34.0	37.0	29.0	39.0
78-79	33.94600256934268	35.0	34.0	37.0	29.0	39.0
80-81	33.68546300737947	35.0	34.0	36.0	28.0	39.0
82-83	33.437490225326755	35.0	34.0	36.0	28.0	37.0
84-85	33.22189592519336	35.0	34.0	35.5	28.0	37.0
86-87	33.038974661418465	35.0	33.5	35.0	27.0	37.0
88-89	32.90022539246545	35.0	33.0	35.0	27.0	36.0
90-91	32.7344840615328	35.0	33.0	35.0	27.0	36.0
92-93	32.64042525578431	35.0	33.0	35.0	27.0	36.0
94-95	32.54497072526433	35.0	33.0	35.0	27.0	36.0
96-97	32.459375308025415	35.0	33.0	35.0	26.0	35.0
98-99	32.383577727544534	35.0	33.0	35.0	26.0	35.0
100	31.45688564125142	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2017977026036597
1101	2	0.22585906959718827
1101	3	0.29739727425601714
1101	4	0.21448312464396935
1101	5	0.18716823898061818
1101	6	0.26844086378603293
1101	7	0.2060563303571712
1101	8	0.2786414245009965
1101	9	0.33518222054586744
1101	10-11	0.377527096013587
1101	12-13	0.36395075233636476
1101	14-15	0.4180945675835588
1101	16-17	0.36471336030965773
1101	18-19	0.3265929433043908
1101	20-21	0.32002609809798344
1101	22-23	0.3355971853364039
1101	24-25	0.33578956323475495
1101	26-27	0.3578895298690341
1101	28-29	0.4317185256153664
1101	30-31	0.4215013439744908
1101	32-33	0.40408120942784365
1101	34-35	0.3833078235072591
1101	36-37	0.368990397702035
1101	38-39	0.3133952415474539
1101	40-41	0.34464379440281334
1101	42-43	0.24748474012245225
1101	44-45	0.3039420561786699
1101	46-47	0.3086423903887052
1101	48-49	0.3238178994305372
1101	50-51	0.3418827115012135
1101	52-53	0.23150876017704292
1101	54-55	0.3609974753215539
1101	56-57	0.2571431414396699
1101	58-59	0.3066469248120569
1101	60-61	0.28937414226810176
1101	62-63	0.30982826954022613
1101	64-65	0.21017302221491008
1101	66-67	0.2710885845211024
1101	68-69	0.2662715309877086
1101	70-71	0.21151309191861856
1101	72-73	0.22480112100851102
1101	74-75	0.22135109642194806
1101	76-77	0.2068867927096747
1101	78-79	0.19283900119823727
1101	80-81	0.2352924871926163
1101	82-83	0.21469793233376322
1101	84-85	0.22122343612394246
1101	86-87	0.21420587046051764
1101	88-89	0.24183265850783897
1101	90-91	0.2655917256899585
1101	92-93	0.2754432823668296
1101	94-95	0.23460516777480933
1101	96-97	0.26057043426204984
1101	98-99	0.20103670519455363
1101	100	0.14646303691794316
1102	1	0.16052394149746974
1102	2	0.205436861790794
1102	3	0.3005184586413243
1102	4	0.27594980957578485
1102	5	0.17282734579918468
1102	6	0.2752871856028918
1102	7	0.25609022048650587
1102	8	0.3626118561234648
1102	9	0.28268590773376445
1102	10-11	0.325404129983319
1102	12-13	0.34707233076220234
1102	14-15	0.405294829025479
1102	16-17	0.41931957498981376
1102	18-19	0.3664288862526135
1102	20-21	0.339864418512164
1102	22-23	0.41821630920713915
1102	24-25	0.32849564841045265
1102	26-27	0.34618586526575257
1102	28-29	0.4344236534577135
1102	30-31	0.36883991099457347
1102	32-33	0.37245010249397126
1102	34-35	0.3837915809718808
1102	36-37	0.3676873202455653
1102	38-39	0.35010752082855845
1102	40-41	0.3707580365346317
1102	42-43	0.3372017172128281
1102	44-45	0.27623730518715917
1102	46-47	0.22321424023969172
1102	48-49	0.2091933301313773
1102	50-51	0.209061205510082
1102	52-53	0.24277157541937555
1102	54-55	0.28412621920235637
1102	56-57	0.22005364769711377
1102	58-59	0.2390989252552913
1102	60-61	0.20226100609431086
1102	62-63	0.1828422987050402
1102	64-65	0.08739419425008776
1102	66-67	0.1197443311525106
1102	68-69	0.09702828448521217
1102	70-71	0.08067015197436689
1102	72-73	0.12538304480953855
1102	74-75	0.0911260596161938
1102	76-77	0.06680571821142678
1102	78-79	0.101894746560248
1102	80-81	0.06664747485967126
1102	82-83	0.04833835502023476
1102	84-85	0.14109873419972274
1102	86-87	0.11905827451759876
1102	88-89	0.07957087120879436
1102	90-91	0.06353495317801361
1102	92-93	0.03785469928100582
1102	94-95	-0.02571251218864745
1102	96-97	0.05197318413597429
1102	98-99	0.03235956136949625
1102	100	-0.13743833183073306
1103	1	0.12246922491751988
1103	2	0.1503137309131617
1103	3	0.16920423858122646
1103	4	0.10035829975272037
1103	5	0.06630370205768799
1103	6	0.11449341221084808
1103	7	0.12300896409812623
1103	8	0.12968180747568425
1103	9	0.2438220600251242
1103	10-11	0.29373453010423844
1103	12-13	0.25665253554909384
1103	14-15	0.30222866138918647
1103	16-17	0.27080753985274697
1103	18-19	0.24522332954426673
1103	20-21	0.1904053336232039
1103	22-23	0.2301174663530361
1103	24-25	0.2754956701853928
1103	26-27	0.30065056040378835
1103	28-29	0.3007596932879153
1103	30-31	0.2894156275822084
1103	32-33	0.21887645068294148
1103	34-35	0.2188962415774327
1103	36-37	0.21150640764504658
1103	38-39	0.26050844869715206
1103	40-41	0.23074213442450997
1103	42-43	0.21484644370678296
1103	44-45	0.1845194674130184
1103	46-47	0.236009386733798
1103	48-49	0.20373713874759858
1103	50-51	0.16860865182204776
1103	52-53	0.20074847220872982
1103	54-55	0.19314059578584875
1103	56-57	0.1968740006048364
1103	58-59	0.26874246309373007
1103	60-61	0.17857243383866006
1103	62-63	0.12683949229961655
1103	64-65	0.16143683342396997
1103	66-67	0.15764563047594748
1103	68-69	0.22252462501452186
1103	70-71	0.14972629801503956
1103	72-73	0.18558966336639315
1103	74-75	0.20419899669686004
1103	76-77	0.1332706784913782
1103	78-79	0.11944842178575499
1103	80-81	0.15240179718514213
1103	82-83	0.20471133539460595
1103	84-85	0.22767691775990073
1103	86-87	0.22671600833562167
1103	88-89	0.14683128923639543
1103	90-91	0.19607170799476137
1103	92-93	0.22418381596137493
1103	94-95	0.18136684081385823
1103	96-97	0.1660731938706519
1103	98-99	0.10606324799481825
1103	100	0.14689383077939056
1104	1	0.23974433332166
1104	2	0.2379719508294471
1104	3	0.25063705307855955
1104	4	0.23833797240981625
1104	5	0.19740002508949317
1104	6	0.1709130678240527
1104	7	0.15786872799014873
1104	8	0.21422387830808276
1104	9	0.2914966972158908
1104	10-11	0.313357559404686
1104	12-13	0.31202010704569716
1104	14-15	0.44596252609079556
1104	16-17	0.3566664390180989
1104	18-19	0.33210924828502186
1104	20-21	0.33877488521856236
1104	22-23	0.3349173259916043
1104	24-25	0.35438161399068946
1104	26-27	0.3198857159292672
1104	28-29	0.31592405401679713
1104	30-31	0.3354761083201083
1104	32-33	0.3736587651426788
1104	34-35	0.35631760157944115
1104	36-37	0.3562882834753083
1104	38-39	0.3597897229784266
1104	40-41	0.3657619980734097
1104	42-43	0.28370093151288245
1104	44-45	0.2483124844318425
1104	46-47	0.28752140518130176
1104	48-49	0.36193355702465624
1104	50-51	0.3157359420944985
1104	52-53	0.25604993597793424
1104	54-55	0.26932740181135983
1104	56-57	0.1852725644066311
1104	58-59	0.26221659648721385
1104	60-61	0.25167442145677654
1104	62-63	0.2290824554296833
1104	64-65	0.21641433799825194
1104	66-67	0.24451087097022395
1104	68-69	0.20666190350664237
1104	70-71	0.20519538090347567
1104	72-73	0.14253943402584213
1104	74-75	0.1528088545809112
1104	76-77	0.17757068851719282
1104	78-79	0.1612961797625445
1104	80-81	0.1571828584178121
1104	82-83	0.1886991665598572
1104	84-85	0.16677258911364135
1104	86-87	0.21316486413931557
1104	88-89	0.19786833462828923
1104	90-91	0.19680108614349479
1104	92-93	0.19465089368652144
1104	94-95	0.17889234398452203
1104	96-97	0.1634129061297216
1104	98-99	0.1683235683196571
1104	100	0.16927771929231383
1105	1	0.15943408350814536
1105	2	0.11807820910708955
1105	3	0.18039698244268365
1105	4	0.14135522035087433
1105	5	0.14696996830677733
1105	6	0.12607298805422573
1105	7	0.05351792344144002
1105	8	0.09885144152261915
1105	9	0.23849543368623216
1105	10-11	0.19327428823294923
1105	12-13	0.22274336360311509
1105	14-15	0.2665238072794267
1105	16-17	0.21859004875489063
1105	18-19	0.21225889282215604
1105	20-21	0.3122833874030988
1105	22-23	0.17946511938166765
1105	24-25	0.18696697835915188
1105	26-27	0.19667939523630906
1105	28-29	0.20394259593400932
1105	30-31	0.21560520147117046
1105	32-33	0.24958776390958803
1105	34-35	0.20997574830111176
1105	36-37	0.20563748958287675
1105	38-39	0.19789732090197276
1105	40-41	0.22851451371529663
1105	42-43	0.14787225395651404
1105	44-45	0.1295840824162866
1105	46-47	0.1251281582145296
1105	48-49	0.15419481842306482
1105	50-51	0.15946548585372966
1105	52-53	0.08590805529775736
1105	54-55	0.1696798200244558
1105	56-57	0.11090166516931532
1105	58-59	0.11457543891136623
1105	60-61	0.11431732970624608
1105	62-63	0.035839294474264705
1105	64-65	0.058199111704659856
1105	66-67	0.12649415584288448
1105	68-69	0.12331461552843592
1105	70-71	0.11923426108128155
1105	72-73	0.17629318275089645
1105	74-75	0.13980058835673503
1105	76-77	0.08852609962102775
1105	78-79	0.09320867652760256
1105	80-81	0.14539854140887343
1105	82-83	0.1362185381441492
1105	84-85	0.12236256612653307
1105	86-87	0.12788270122010914
1105	88-89	0.04793303343630129
1105	90-91	0.1407833358352235
1105	92-93	0.12442680746203649
1105	94-95	-0.012677781911740738
1105	96-97	0.08122715204608255
1105	98-99	0.12734258215758842
1105	100	0.050647507232454814
1106	1	0.050692788843079484
1106	2	-0.004630758718182193
1106	3	0.034607583802191755
1106	4	0.089055491908276
1106	5	0.04476519239116783
1106	6	-1.1638152833626236E-4
1106	7	-0.00643273437317049
1106	8	0.01101144938688492
1106	9	0.047867504142836026
1106	10-11	0.02284914493805701
1106	12-13	-0.005598328215874915
1106	14-15	0.022866561380155304
1106	16-17	-0.014701803407795921
1106	18-19	3.750706302767526E-4
1106	20-21	0.05792012364315724
1106	22-23	0.046051757648648106
1106	24-25	0.03089576542546979
1106	26-27	0.08196525587884906
1106	28-29	0.011860984938074637
1106	30-31	0.08450746126660391
1106	32-33	0.029568018807410112
1106	34-35	0.09557806323516616
1106	36-37	0.0944232394176936
1106	38-39	0.08177253484424085
1106	40-41	4.006083586176601E-4
1106	42-43	-0.02045834193351226
1106	44-45	-0.006845663594233997
1106	46-47	0.011309358231294198
1106	48-49	-0.07326907271319527
1106	50-51	-0.07498827755429716
1106	52-53	-0.027381850286701592
1106	54-55	0.03412181193911579
1106	56-57	-0.009778119355949855
1106	58-59	-0.054842231060476365
1106	60-61	-0.04290594249174973
1106	62-63	0.007528436107968162
1106	64-65	-0.04161701893304581
1106	66-67	-0.02223933410446932
1106	68-69	-0.004105926891320166
1106	70-71	0.002486174828597143
1106	72-73	0.0264460941574427
1106	74-75	-0.005134096798308008
1106	76-77	-0.018113691707519308
1106	78-79	-0.05924282655449531
1106	80-81	-0.020349806949816696
1106	82-83	0.015662119908711247
1106	84-85	0.09768532947513364
1106	86-87	0.07244132759845456
1106	88-89	-0.020484157036378292
1106	90-91	0.01607733897768071
1106	92-93	0.032580063363127465
1106	94-95	0.015895020059865317
1106	96-97	0.022781276069096634
1106	98-99	-0.00934583248653098
1106	100	-0.07777230754813758
1107	1	0.18778764444628848
1107	2	0.2270045742111897
1107	3	0.2574363815590779
1107	4	0.1755196275071782
1107	5	0.1989107225188249
1107	6	0.21551359620560362
1107	7	0.18617959523663075
1107	8	0.1977850459726369
1107	9	0.2816040174535246
1107	10-11	0.3695280440976205
1107	12-13	0.2974182114921149
1107	14-15	0.3160138476258538
1107	16-17	0.3327620447500337
1107	18-19	0.32398143817913194
1107	20-21	0.2861607913907065
1107	22-23	0.295372289850107
1107	24-25	0.27337691966434363
1107	26-27	0.3144096660712208
1107	28-29	0.2757390485176643
1107	30-31	0.31777264184709253
1107	32-33	0.30963243794796824
1107	34-35	0.27249276897499186
1107	36-37	0.24999087872209458
1107	38-39	0.2673155899287849
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2216	44-45	-0.3770958642105313
2216	46-47	-0.2846302470734585
2216	48-49	-0.2709909349225512
2216	50-51	-0.2543251853168016
2216	52-53	-0.2385509517763964
2216	54-55	-0.27999648579412906
2216	56-57	-0.19469026572318882
2216	58-59	-0.20142044370640377
2216	60-61	-0.24961245531583387
2216	62-63	-0.2234289065616295
2216	64-65	-0.2831374011153329
2216	66-67	-0.28292907715914595
2216	68-69	-0.2317056398361217
2216	70-71	-0.29595193044873014
2216	72-73	-0.21230070290933867
2216	74-75	-0.25071902078278185
2216	76-77	-0.2048694533114528
2216	78-79	-0.2685930446452005
2216	80-81	-0.2281796956395752
2216	82-83	-0.17691024192335192
2216	84-85	-0.18929560118467492
2216	86-87	-0.15795029313429154
2216	88-89	-0.25703419386079673
2216	90-91	-0.27359911693490346
2216	92-93	-0.20071948989920685
2216	94-95	-0.16822459532235712
2216	96-97	-0.17870210637917694
2216	98-99	-0.16177495324518532
2216	100	-0.16991559461672168
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	83.0
1	38.0
2	31.0
3	39.0
4	48.0
5	37.0
6	140.0
7	817.0
8	2442.0
9	3189.0
10	3397.0
11	3770.0
12	4446.0
13	5242.0
14	6532.0
15	7257.0
16	7437.0
17	8331.0
18	9084.0
19	10235.0
20	11394.0
21	12696.0
22	14324.0
23	16499.0
24	20419.0
25	25137.0
26	28065.0
27	26860.0
28	28231.0
29	35626.0
30	41242.0
31	44845.0
32	56835.0
33	76964.0
34	117850.0
35	184257.0
36	349323.0
37	751611.0
38	853038.0
39	275235.0
40	534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.513227287102644	20.483638403086577	13.083195641553836	23.919938668256943
2	15.152695095796092	21.23523019682337	39.7734094746897	23.838665232690836
3	17.544352546472005	27.334772593592994	30.764812386838987	24.356062473096017
4	12.07646813154243	18.00521603637096	39.86968781000405	30.048628022082557
5	12.70150153702581	39.13440226865338	35.369502319093456	12.794593875227351
6	28.854981798872114	39.111953202169545	18.71325025022854	13.319814748729799
7	25.704840727204864	33.73710238479587	23.5624968580468	16.99556002995247
8	25.9120915733861	34.666034920617186	21.15687728628709	18.264996219709623
9	26.697343297323776	15.403835038850167	21.016580692583425	36.88224097124263
10-11	24.19822936619511	26.795659487736224	28.791471043563995	20.21464010250467
12-13	25.008423970768206	25.01290908831032	27.991865015376728	21.98680192554475
14-15	22.60181448962961	26.043232959175626	25.58548671170579	25.76946583948897
16-17	22.070044203769438	28.668013344100178	26.563302738727874	22.698639713402507
18-19	21.867957482973917	28.529721650072265	27.94464184234094	21.65767902461288
20-21	23.389132523061914	27.61914494180572	27.00550226417407	21.98622027095829
22-23	23.530007054375865	27.504426645498064	26.59739556517313	22.368170734952937
24-25	22.895074278063227	27.73661577890314	27.074590207699288	22.293719735334346
26-27	22.59069269381952	27.98204792804977	26.620577327541195	22.806682050589515
28-29	22.42635588741355	28.27311593746935	26.799864033802944	22.500664141314157
30-31	22.801259744098257	27.815772694032976	26.7198465884176	22.663120973451168
32-33	22.753324215959523	28.20983256847724	26.206299531359978	22.830543684203263
34-35	23.165087232137033	27.98167933713861	26.75308360638473	22.100149824339628
36-37	23.14511634387627	27.573275216164845	26.562890233739328	22.718718206219553
38-39	22.819854444729796	27.646398589040917	26.86264196962475	22.671104996604544
40-41	22.818123682652185	27.234931709872278	27.202004176078837	22.7449404313967
42-43	22.707514064099033	27.272948357035865	26.8472296074672	23.172307971397903
44-45	22.62945508569648	27.881670643065824	26.96705227796813	22.521821993269565
46-47	22.85650385301647	27.664533350686447	26.557269723641745	22.921693072655334
48-49	22.923839396153888	27.459965138706504	26.720111666569984	22.896083798569624
50-51	22.928823867163917	27.49891786878818	26.479758969857098	23.092499294190805
52-53	22.70883946741548	27.708491089410884	26.57433992227884	23.008329520894794
54-55	22.52178847500478	27.639649938216753	26.635998818731295	23.202562768047173
56-57	23.089822752590543	27.350518371267025	26.53220436140512	23.02745451473731
58-59	22.617491091166894	27.18942667279324	27.08911396798539	23.10396826805448
60-61	22.53571077000241	27.699698714644	26.696794654465155	23.067795860888435
62-63	22.822191546703475	27.326365608361268	26.55976796161178	23.291674883323473
64-65	22.26031376246929	27.46347646502107	26.74432038262962	23.531889389880014
66-67	22.700793684053107	27.366709313112835	26.540732286317887	23.39176471651617
68-69	22.54405833314434	27.222703752021026	26.816657446932297	23.41658046790234
70-71	22.53496868673273	27.039651748676746	26.736470099843856	23.68890946474667
72-73	22.782093164699916	27.14335555218483	26.806777831588875	23.267773451526384
74-75	22.729633274367465	27.534529873739416	26.710651129127367	23.025185722765755
76-77	22.69881846575572	27.28894629890294	26.689329379273396	23.322905856067948
78-79	22.547875187532753	27.339724306102102	26.818634276739427	23.293766229625714
80-81	22.578726000255067	27.391296916436254	26.94502623652182	23.084950846786864
82-83	22.95601422280421	26.87096663925444	27.392735694203136	22.78028344373821
84-85	22.95354009568919	27.385056460696404	26.654963798565618	23.00643964504879
86-87	22.472200927334175	27.615446366872987	26.809817614419284	23.102535091373557
88-89	22.89651099313728	26.99463727757618	26.739417439479986	23.369434289806552
90-91	22.709951230751003	26.941308459378487	27.000764315048986	23.347975994821525
92-93	23.05042062793372	27.017885910578826	26.97543774770672	22.95625571378073
94-95	22.241577993496023	26.956156329479636	27.387174661440174	23.41509101558417
96-97	22.548882180072187	26.84013473360664	27.15534248461799	23.455640601703184
98-99	22.24817713524259	27.332413198344412	26.87878193803172	23.540627728381278
100	22.424233461014513	26.864087736855165	26.730819476556455	23.980859325573867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1918.0
1	1406.5
2	993.5
3	1054.5
4	932.5
5	782.0
6	698.0
7	714.5
8	777.5
9	819.5
10	706.0
11	561.5
12	543.5
13	564.0
14	613.0
15	711.5
16	777.0
17	791.5
18	945.5
19	1116.5
20	1377.0
21	1940.5
22	2886.0
23	4131.0
24	5797.5
25	8213.5
26	11994.0
27	16470.5
28	21004.0
29	27350.5
30	34794.0
31	42895.5
32	53712.5
33	61471.5
34	66332.0
35	71056.0
36	76435.5
37	86113.5
38	89931.0
39	89200.5
40	88626.5
41	86799.0
42	86720.5
43	90252.5
44	96068.5
45	106774.5
46	117333.5
47	123267.5
48	126527.5
49	123239.0
50	119575.5
51	120751.0
52	120425.0
53	118448.0
54	114625.5
55	103963.0
56	92975.5
57	79626.0
58	67760.5
59	61486.0
60	50059.5
61	37771.0
62	30988.5
63	24691.0
64	17107.5
65	11084.0
66	8006.5
67	6048.5
68	4493.0
69	3490.5
70	2903.0
71	2333.0
72	1900.0
73	1753.5
74	1432.5
75	1128.5
76	882.5
77	644.5
78	504.5
79	358.5
80	270.5
81	212.0
82	157.5
83	118.0
84	90.5
85	77.5
86	60.0
87	33.0
88	22.0
89	18.0
90	19.0
91	20.5
92	15.0
93	11.0
94	8.5
95	6.0
96	6.5
97	6.5
98	7.5
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05789235045571334
2	0.00962682104626788
3	0.01149961558427904
4	0.00896970015573765
5	0.011992456252176714
6	0.011893888118597179
7	0.0029899000519125504
8	0.005355535257821381
9	0.0072611858403590504
10-11	0.006784773194724633
12-13	0.005963372081561845
14-15	0.009856813357953463
16-17	0.009446112801372067
18-19	0.007704742441466957
20-21	0.006456212749459519
22-23	0.002595627517594412
24-25	0.0038605852318651063
26-27	0.004698414367291151
28-29	0.005799091858929287
30-31	0.008657567732735792
32-33	0.010053949625112532
34-35	0.011483187562015783
36-37	0.01747941568810414
38-39	0.017857260200159025
40-41	0.017545127777157162
42-43	0.02385348832624738
44-45	0.027451225201900395
46-47	0.029537584029333878
48-49	0.03122967032244922
50-51	0.03196893132429573
52-53	0.029833288430072484
54-55	0.035763804467107814
56-57	0.03831014791791246
58-59	0.03594451271200363
60-61	0.03665091766932363
62-63	0.032198923635981314
64-65	0.03467955499773293
66-67	0.03688090998100921
68-69	0.03919726112012827
70-71	0.03875370451902037
72-73	0.03428528246341479
74-75	0.03752160284927618
76-77	0.03737375064890688
78-79	0.03651949349121758
80-81	0.0412671919252985
82-83	0.03752160284927618
84-85	0.033546021461568286
86-87	0.03244534396993015
88-89	0.02986614447459899
90-91	0.03241248792540363
92-93	0.03435099455246782
94-95	0.03725875449306409
96-97	0.0323139197918241
98-99	0.02991542854138876
100	0.02776335762490225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3043580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.403051952694646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1795185477232	35.560383859710186
2	14.721675900246467	15.134781422622037
3	6.1967763327094785	9.555996473084818
4	3.2422969993649797	6.666558444176959
5	1.8754316874229306	4.8201456231165345
6	1.1639366626098107	3.589793804266687
7	0.8142386742200398	2.929804701097814
8	0.5839719741908025	2.401435338259798
9	0.3987812018047837	1.8448713750716377
>10	1.7647960038607384	14.650625129177108
>50	0.04464805508153251	1.5369404693631157
>100	0.013703369570747257	1.110845390863045
>500	1.1229559730334533E-4	0.057550194129760084
>1k	1.1229559730334533E-4	0.1402677750604985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	6.571208905302309E-5	0.0
18-19	0.0	0.0	0.0	1.6428022263255773E-4	0.0
20-21	0.0	0.0	0.0	2.9570440073860385E-4	0.0
22-23	0.0	0.0	0.0	6.40692868266975E-4	0.0
24-25	0.0	0.0	0.0	0.0015113780482195309	0.0
26-27	0.0	0.0	0.0	0.002940615985122783	0.0
28-29	0.0	0.0	0.0	0.00896970015573765	0.0
30-31	0.0	0.0	0.0	0.023360647658349706	0.0
32-33	0.0	0.0	0.0	0.049941187680297545	0.0
34-35	0.0	0.0	0.0	0.08908916473363604	0.0
36-37	0.0	0.0	0.0	0.14895287786094008	0.0
38-39	0.0	0.0	0.0	0.23316291998238914	0.0
40-41	0.0	0.0	0.0	0.32647408643768194	0.0
42-43	0.0	0.0	0.0	0.4153004028151059	0.0
44-45	0.0	0.0	0.0	0.5210640101459465	0.0
46-47	0.0	0.0	0.0	0.644865585921842	0.0
48-49	0.0	0.0	0.0	0.775057662358144	0.0
50-51	0.0	0.0	0.0	0.9126094927683845	0.0
52-53	0.0	0.0	0.0	1.0455614769449135	0.0
54-55	0.0	0.0	0.0	1.1798441309247663	0.0
56-57	0.0	0.0	0.0	1.3355489259359046	0.0
58-59	0.0	0.0	0.0	1.5114109042640576	0.0
60-61	0.0	0.0	0.0	1.6955361777906282	0.0
62-63	0.0	0.0	0.0	1.8732709506567922	0.0
64-65	0.0	0.0	0.0	2.052763521905125	0.0
66-67	0.0	0.0	0.0	2.2503269176430387	0.0
68-69	0.0	0.0	0.0	2.468343201098706	0.0
70-71	0.0	0.0	0.0	2.7004054435894576	0.0
72-73	0.0	0.0	0.0	2.931383436610833	0.0
74-75	0.0	0.0	0.0	3.158845832867873	0.0
76-77	0.0	0.0	0.0	3.4004034722267855	0.0
78-79	0.0	0.0	0.0	3.6650917669323624	0.0
80-81	0.0	0.0	0.0	3.9507915021126436	0.0
82-83	0.0	0.0	0.0	4.253477812313131	0.0
84-85	0.0	0.0	0.0	4.546323737177929	0.0
86-87	0.0	0.0	0.0	4.848040794064884	0.0
88	0.0	0.0	0.0	5.0806615893125855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4380	0.0	40.683243	1
GTATCAA	6965	0.0	33.61698	1
CAACGCA	7525	0.0	29.664549	5
ATCAACG	7680	0.0	29.249426	3
TATCAAC	8570	0.0	26.86945	2
AACGCAG	8715	0.0	26.367607	6
GTACATG	13745	0.0	25.825735	1
TACATGG	13530	0.0	25.772087	2
TCAACGC	9050	0.0	24.873539	4
TATTCGC	265	1.8189894E-12	24.826675	9
GTACTAG	1360	0.0	24.54539	1
ACGCAGA	9005	0.0	24.526535	7
ACATGGG	13865	0.0	24.268547	3
CGCAGAG	9230	0.0	24.08218	8
CATGGGG	9220	0.0	22.987738	4
CTGTGCG	2130	0.0	22.062605	9
CGTTAAC	405	0.0	22.057133	1
GTGATCG	1045	0.0	22.035156	8
TGATCGC	1040	0.0	21.689238	9
GTATAAG	1715	0.0	21.657755	1
>>END_MODULE