FastQCFastQC Report
Mon 16 Jan 2017
ERR1146505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043580
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132900.4366568317573384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124440.40886061808790963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70850.23278507547033425No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT31140.10231372265555694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.060.316961
GTATCAA145250.042.9610141
CAACGCA181100.033.879125
ATCAACG181800.033.7492263
TATCAAC187100.033.2493632
AACGCAG195450.031.7415716
TCAACGC195400.031.4237964
GTACTAG13700.029.5409851
ACGCAGA213950.028.6655227
CGCAGAG217650.028.135038
GCAGAGT234000.025.867939
GTACATG132400.025.6978871
TACATGG131250.025.312272
ACATGGG131100.024.3508593
TAGGCAT21100.023.8419285
GAGTACT156900.023.6777612-13
CAGAGTA225950.023.16005110-11
CATGGGG89450.022.916394
GTGGTAT23050.022.866211
AGAGTAC218050.022.25336310-11
Produced by FastQC (version 0.11.5)