##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3043580
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49262184664113	34.0	31.0	34.0	31.0	34.0
2	32.522675599129975	34.0	31.0	34.0	31.0	34.0
3	32.65034301710486	34.0	31.0	34.0	31.0	34.0
4	36.155140656726616	37.0	35.0	37.0	35.0	37.0
5	36.18350987981259	37.0	37.0	37.0	35.0	37.0
6	36.28206355673253	37.0	37.0	37.0	35.0	37.0
7	36.2705540843349	37.0	37.0	37.0	35.0	37.0
8	36.28400600608494	37.0	37.0	37.0	35.0	37.0
9	38.091288219793796	39.0	39.0	39.0	37.0	39.0
10-11	38.0619461292294	39.0	38.0	39.0	36.0	39.0
12-13	38.044314425774914	39.0	38.0	39.0	35.0	39.0
14-15	39.48046741008943	41.0	39.0	41.0	36.5	41.0
16-17	39.482538490856165	41.0	39.0	41.0	36.5	41.0
18-19	39.55066221357743	41.0	39.5	41.0	37.0	41.0
20-21	39.58584824450154	41.0	40.0	41.0	37.0	41.0
22-23	39.5402647540068	41.0	39.5	41.0	36.5	41.0
24-25	39.518238226036445	41.0	39.0	41.0	36.0	41.0
26-27	39.43802413605031	41.0	39.0	41.0	36.0	41.0
28-29	39.34562669619329	41.0	39.0	41.0	36.0	41.0
30-31	39.214898244830096	41.0	39.0	41.0	35.5	41.0
32-33	39.05930203904612	41.0	39.0	41.0	35.0	41.0
34-35	38.964798362454744	41.0	39.0	41.0	35.0	41.0
36-37	38.864447459899196	41.0	38.0	41.0	35.0	41.0
38-39	38.744311797291346	40.5	38.0	41.0	35.0	41.0
40-41	38.63710991661136	40.0	38.0	41.0	35.0	41.0
42-43	38.520720828760865	40.0	38.0	41.0	35.0	41.0
44-45	38.33521100151795	40.0	38.0	41.0	34.5	41.0
46-47	38.047501954934646	40.0	38.0	41.0	33.5	41.0
48-49	37.77950998495193	40.0	37.5	41.0	33.0	41.0
50-51	37.601846181141944	40.0	37.0	41.0	33.0	41.0
52-53	37.45731851963806	40.0	37.0	41.0	33.0	41.0
54-55	37.303898698243515	40.0	36.0	41.0	33.0	41.0
56-57	37.07978039019838	39.5	35.5	41.0	32.5	41.0
58-59	36.852005040117234	39.0	35.0	41.0	32.0	41.0
60-61	36.65699373763792	39.0	35.0	41.0	32.0	41.0
62-63	36.39163189401954	38.5	35.0	41.0	31.5	41.0
64-65	36.09117371647862	37.5	35.0	40.5	31.0	41.0
66-67	35.83113241643065	37.0	35.0	40.0	31.0	41.0
68-69	35.52063244600109	36.5	35.0	40.0	31.0	41.0
70-71	35.24701864251966	36.0	35.0	39.0	31.0	41.0
72-73	34.942245973491744	35.5	35.0	39.0	30.5	41.0
74-75	34.63201230130307	35.0	35.0	38.5	30.0	40.5
76-77	34.34639996320123	35.0	35.0	37.0	30.0	39.5
78-79	34.076641487984546	35.0	35.0	37.0	30.0	39.0
80-81	33.84551991404859	35.0	34.5	36.0	29.5	39.0
82-83	33.60505884517575	35.0	34.0	36.0	29.0	37.0
84-85	33.40988523383647	35.0	34.0	36.0	29.0	37.0
86-87	33.24061697080412	35.0	34.0	35.0	29.0	37.0
88-89	33.10334786665703	35.0	34.0	35.0	29.0	36.0
90-91	33.022794538011155	35.0	34.0	35.0	29.0	36.0
92-93	32.94301858337879	35.0	34.0	35.0	29.0	36.0
94-95	32.875888920284666	35.0	34.0	35.0	29.0	36.0
96-97	32.82465632577425	35.0	34.0	35.0	29.0	35.5
98-99	32.775548038822706	35.0	34.0	35.0	29.0	35.0
100	32.22725836022053	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2433314964510629
1101	2	0.2291904246078147
1101	3	0.2485374701545453
1101	4	0.16032543922874254
1101	5	0.15418721318573603
1101	6	0.1382680351258756
1101	7	0.14546877338847963
1101	8	0.1995674674543153
1101	9	0.18386176056080927
1101	10-11	0.20920889953259092
1101	12-13	0.24855076190261371
1101	14-15	0.3451250457974595
1101	16-17	0.33656120082909524
1101	18-19	0.32821988684277414
1101	20-21	0.3131222730431702
1101	22-23	0.3919933276004528
1101	24-25	0.3272682538595575
1101	26-27	0.37824677919570604
1101	28-29	0.37353477833682547
1101	30-31	0.37604907121978215
1101	32-33	0.31833226513646196
1101	34-35	0.3232410071852243
1101	36-37	0.31601051965711946
1101	38-39	0.36509713259694365
1101	40-41	0.29935114809246954
1101	42-43	0.35776887334188956
1101	44-45	0.3880476212395436
1101	46-47	0.27105586409501825
1101	48-49	0.19233721354643052
1101	50-51	0.2096671155567833
1101	52-53	0.21347571171350666
1101	54-55	0.2551550845111734
1101	56-57	0.1693511814431119
1101	58-59	0.17164803133611883
1101	60-61	0.1671346176601105
1101	62-63	0.23080646981697583
1101	64-65	0.25963169537979525
1101	66-67	0.2640801518393232
1101	68-69	0.252500481596293
1101	70-71	0.2710747775941371
1101	72-73	0.1969272502686863
1101	74-75	0.19924528835593946
1101	76-77	0.16482051488327443
1101	78-79	0.10605682637734759
1101	80-81	0.12102057907000585
1101	82-83	0.1453447574688056
1101	84-85	0.15687124602222724
1101	86-87	0.16487824142432572
1101	88-89	0.13336448404044177
1101	90-91	0.11664657781243903
1101	92-93	0.14347155231027386
1101	94-95	0.12342812937695413
1101	96-97	0.16065616387807324
1101	98-99	0.1110940454178575
1101	100	0.15147516945479111
1102	1	0.24042068739066735
1102	2	0.2554583160969912
1102	3	0.2671022000371224
1102	4	0.17523749282248247
1102	5	0.12798338633825068
1102	6	0.14092423847405655
1102	7	0.16601236683175102
1102	8	0.15955959457760116
1102	9	0.1756213551169168
1102	10-11	0.21828852533690934
1102	12-13	0.2444787233617447
1102	14-15	0.32223482865997255
1102	16-17	0.2865424177901801
1102	18-19	0.27163563502919885
1102	20-21	0.2880648660995462
1102	22-23	0.27855481365683943
1102	24-25	0.24544656618665783
1102	26-27	0.2736267069831513
1102	28-29	0.25550549398581524
1102	30-31	0.27333880630555285
1102	32-33	0.2614827301703926
1102	34-35	0.30768100155758304
1102	36-37	0.3167753285064734
1102	38-39	0.27781328960701757
1102	40-41	0.2786211126579161
1102	42-43	0.24462056759711714
1102	44-45	0.24767342951846416
1102	46-47	0.24220112303385122
1102	48-49	0.24175445670672246
1102	50-51	0.2797152889938559
1102	52-53	0.21521094451608747
1102	54-55	0.2384781459045371
1102	56-57	0.2802729268469193
1102	58-59	0.2714053724564707
1102	60-61	0.27549761201898093
1102	62-63	0.28524015954100435
1102	64-65	0.2857549620973572
1102	66-67	0.17456578165755587
1102	68-69	0.219072358157419
1102	70-71	0.24275810679753818
1102	72-73	0.20786154414410873
1102	74-75	0.15742793645104314
1102	76-77	0.21002165670163464
1102	78-79	0.18996811886463405
1102	80-81	0.16257790389982318
1102	82-83	0.1473767768312655
1102	84-85	0.19383171793996468
1102	86-87	0.1418274659975367
1102	88-89	0.1257142468359831
1102	90-91	0.15932506692886506
1102	92-93	0.1578365110537021
1102	94-95	0.15064548662387978
1102	96-97	0.14836031455282495
1102	98-99	0.19191995434901798
1102	100	0.04555734197737138
1103	1	0.1874167704633436
1103	2	0.23107896591241683
1103	3	0.23805859379975658
1103	4	0.1543167074900822
1103	5	0.176598500195567
1103	6	0.16000574591468109
1103	7	0.12039188353056574
1103	8	0.14343339471439975
1103	9	0.18323163237593576
1103	10-11	0.19540690671599492
1103	12-13	0.20767010970808997
1103	14-15	0.2867073179454138
1103	16-17	0.259758633971785
1103	18-19	0.27088638498372575
1103	20-21	0.2881025239358337
1103	22-23	0.31153401602800557
1103	24-25	0.2794353892383725
1103	26-27	0.30388375373778587
1103	28-29	0.3053121453331755
1103	30-31	0.3207133890592857
1103	32-33	0.328748207669193
1103	34-35	0.3520565347233813
1103	36-37	0.37039534812953434
1103	38-39	0.36141813722086624
1103	40-41	0.39245726136282144
1103	42-43	0.4088454630133711
1103	44-45	0.3579713106887823
1103	46-47	0.27850180420518456
1103	48-49	0.37794603902751334
1103	50-51	0.3778578866672646
1103	52-53	0.4321434895966121
1103	54-55	0.3661083344242755
1103	56-57	0.3851793010121014
1103	58-59	0.3485739642839576
1103	60-61	0.42832275028985123
1103	62-63	0.4196230769729894
1103	64-65	0.41449339896412596
1103	66-67	0.3927442695545338
1103	68-69	0.3888190899951951
1103	70-71	0.2407188196834369
1103	72-73	0.2076681314475337
1103	74-75	0.2849414048914696
1103	76-77	0.22969185603199094
1103	78-79	0.26835130400029783
1103	80-81	0.2377726935411033
1103	82-83	0.25144788994054323
1103	84-85	0.27810969937746677
1103	86-87	0.21012094478746945
1103	88-89	0.20334478759738772
1103	90-91	0.2332445175855682
1103	92-93	0.20574377927228227
1103	94-95	0.20959809282793884
1103	96-97	0.22167208429180363
1103	98-99	0.23150817103271493
1103	100	0.14721146884073022
1104	1	0.15779173599960217
1104	2	0.15339768661823427
1104	3	0.18522943371252865
1104	4	0.08741631397678873
1104	5	0.0736245923829486
1104	6	0.10741031267408374
1104	7	0.09326139752911189
1104	8	0.13940489633106523
1104	9	0.1798673443342267
1104	10-11	0.17209563017891583
1104	12-13	0.15553837047957586
1104	14-15	0.22667304218533957
1104	16-17	0.22130304833877545
1104	18-19	0.2562418001778468
1104	20-21	0.21782656333729022
1104	22-23	0.2385532781279096
1104	24-25	0.19629066657752503
1104	26-27	0.20330350960916377
1104	28-29	0.24840999730199798
1104	30-31	0.21439837790556027
1104	32-33	0.2685882958963788
1104	34-35	0.279893214172958
1104	36-37	0.2612530567291813
1104	38-39	0.2940086202145764
1104	40-41	0.2719334751132223
1104	42-43	0.31349023580330027
1104	44-45	0.351801673873986
1104	46-47	0.3310540011122285
1104	48-49	0.30501856327746424
1104	50-51	0.29183888774504396
1104	52-53	0.3243424644568762
1104	54-55	0.37592016238127
1104	56-57	0.39970576916760336
1104	58-59	0.3799898602159857
1104	60-61	0.33752237682818986
1104	62-63	0.2967363311023803
1104	64-65	0.28790744012010094
1104	66-67	0.3368027200645969
1104	68-69	0.29026844708971566
1104	70-71	0.24252346664937363
1104	72-73	0.2488754903968271
1104	74-75	0.26825030029714014
1104	76-77	0.2824238910220913
1104	78-79	0.25768195542772077
1104	80-81	0.24639207595080848
1104	82-83	0.2805117919883173
1104	84-85	0.24319604672188433
1104	86-87	0.2868350460983393
1104	88-89	0.24880246611958512
1104	90-91	0.20787606883002496
1104	92-93	0.2467434158364341
1104	94-95	0.24795028722852663
1104	96-97	0.2468841929716561
1104	98-99	0.24956748626945569
1104	100	0.1800760952537388
1105	1	0.11989802472373867
1105	2	0.08367977342119559
1105	3	0.12131005603895062
1105	4	0.09584847156785514
1105	5	0.07768463287678884
1105	6	0.07484169387946338
1105	7	0.14433387541734533
1105	8	0.1292646902082737
1105	9	0.13612333848850255
1105	10-11	0.13954984625771516
1105	12-13	0.10683995826443748
1105	14-15	0.15063976416314517
1105	16-17	0.15490684806171373
1105	18-19	0.1189585411847105
1105	20-21	0.12634168763511866
1105	22-23	0.17946956548195203
1105	24-25	0.1266161396399852
1105	26-27	0.1420619014251443
1105	28-29	0.18912609893239818
1105	30-31	0.21024699791804125
1105	32-33	0.1738585846814118
1105	34-35	0.16905426092355214
1105	36-37	0.14320437277905285
1105	38-39	0.1841256869989678
1105	40-41	0.16246461426894143
1105	42-43	0.09096211085087447
1105	44-45	0.07175783091702215
1105	46-47	0.11719703875932197
1105	48-49	0.22488338082681736
1105	50-51	0.22399647881812967
1105	52-53	0.23408914959442484
1105	54-55	0.2335241752296895
1105	56-57	0.18985328244988864
1105	58-59	0.12557283453435986
1105	60-61	0.1320937662541155
1105	62-63	0.13712817753024353
1105	64-65	0.13831085621716
1105	66-67	0.1095394035275774
1105	68-69	0.17345056281685345
1105	70-71	0.13268544201815757
1105	72-73	0.09727324344527943
1105	74-75	0.07419269552313068
1105	76-77	0.08924086190000935
1105	78-79	0.0841293058212429
1105	80-81	0.12337452354476852
1105	82-83	0.14616053074214364
1105	84-85	0.12110787366398057
1105	86-87	0.12920972070138959
1105	88-89	0.14092671827747694
1105	90-91	0.1236770400733036
1105	92-93	0.1280606835677105
1105	94-95	0.14942313656403172
1105	96-97	0.17497260960153227
1105	98-99	0.1285639696989449
1105	100	0.1251780149054369
1106	1	0.10149874645171764
1106	2	0.03903568381407041
1106	3	0.07512245331715661
1106	4	0.037576872804621075
1106	5	8.44052761834746E-4
1106	6	0.017961768633263375
1106	7	0.025297964296939313
1106	8	0.025966398530954393
1106	9	0.014114619160501718
1106	10-11	0.09507808512083926
1106	12-13	0.09965951511800597
1106	14-15	0.14198436578097073
1106	16-17	0.11989553289910759
1106	18-19	0.15830304991243338
1106	20-21	0.15497357442697535
1106	22-23	0.2051585789316448
1106	24-25	0.15540065094004518
1106	26-27	0.16274386204731428
1106	28-29	0.2242042668233708
1106	30-31	0.20412423530564183
1106	32-33	0.12831615138716757
1106	34-35	0.11531332839999209
1106	36-37	0.1555736973477977
1106	38-39	0.15481937256119238
1106	40-41	0.11823108702481022
1106	42-43	0.08151008236587387
1106	44-45	0.05684198578106958
1106	46-47	0.03408256014726874
1106	48-49	0.011447519767465053
1106	50-51	-0.05453312600592142
1106	52-53	-0.03257258025538334
1106	54-55	-0.0420386029466826
1106	56-57	-0.029322304860798454
1106	58-59	-0.040539468738629125
1106	60-61	-0.05724168622950998
1106	62-63	-0.0010769550054234855
1106	64-65	0.028451632364891566
1106	66-67	-0.03311793125605078
1106	68-69	-0.080544713212376
1106	70-71	-0.06088601044857711
1106	72-73	-0.11811203776002799
1106	74-75	-0.11791736171471712
1106	76-77	-0.11775995040123632
1106	78-79	-0.08219597887664776
1106	80-81	-0.05176714740100863
1106	82-83	-0.08131721812566184
1106	84-85	-0.04631138691561176
1106	86-87	-0.06881926599204036
1106	88-89	-0.08916305059180019
1106	90-91	-0.06445877132556888
1106	92-93	-0.11790782625303109
1106	94-95	-0.12846378889360466
1106	96-97	-0.14655518148363456
1106	98-99	-0.08099686904458281
1106	100	-0.16328788116309312
1107	1	0.11715008396868143
1107	2	0.07630041979994218
1107	3	0.1311133325772218
1107	4	0.08050041609070036
1107	5	0.10317803635573597
1107	6	0.08845012727005752
1107	7	0.09458118903260981
1107	8	0.12193686573962026
1107	9	0.11171741593086892
1107	10-11	0.07399763217266297
1107	12-13	0.07078936832143512
1107	14-15	0.12667212889227386
1107	16-17	0.17158600313519656
1107	18-19	0.10042417871402165
1107	20-21	0.08293079775138779
1107	22-23	0.1194009690739648
1107	24-25	0.10920244412811542
1107	26-27	0.1401573755233514
1107	28-29	0.20141938097308554
1107	30-31	0.17784481935600382
1107	32-33	0.17422494695588142
1107	34-35	0.1924698131692253
1107	36-37	0.17793775862755723
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2216	36-37	-0.48803239686353805
2216	38-39	-0.37609389767202117
2216	40-41	-0.3221702172026255
2216	42-43	-0.3744995939656306
2216	44-45	-0.3705302206475878
2216	46-47	-0.3232733424960017
2216	48-49	-0.3251885318248
2216	50-51	-0.28869382602249516
2216	52-53	-0.29651252411485984
2216	54-55	-0.35357462914734583
2216	56-57	-0.3643311070952606
2216	58-59	-0.29917136078265116
2216	60-61	-0.2994192340564936
2216	62-63	-0.31932837260909963
2216	64-65	-0.21797051710701965
2216	66-67	-0.25249447782061907
2216	68-69	-0.2862145247056347
2216	70-71	-0.2808282035480545
2216	72-73	-0.22188458553328871
2216	74-75	-0.24296315425795
2216	76-77	-0.26185300921871146
2216	78-79	-0.339271970652554
2216	80-81	-0.2811045547013862
2216	82-83	-0.2601297807681817
2216	84-85	-0.3739839569889867
2216	86-87	-0.2951956766446031
2216	88-89	-0.260393457632226
2216	90-91	-0.2593521558040379
2216	92-93	-0.35953233852026756
2216	94-95	-0.3340722797557376
2216	96-97	-0.28604549827171155
2216	98-99	-0.31195242091801845
2216	100	-0.46564345675043484
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	25.0
8	93.0
9	160.0
10	262.0
11	300.0
12	266.0
13	264.0
14	372.0
15	722.0
16	1438.0
17	2599.0
18	4692.0
19	7508.0
20	10649.0
21	15582.0
22	23025.0
23	27305.0
24	26062.0
25	21347.0
26	18198.0
27	18463.0
28	21247.0
29	25032.0
30	29669.0
31	38056.0
32	49397.0
33	66431.0
34	99077.0
35	164184.0
36	295914.0
37	734952.0
38	970275.0
39	368753.0
40	1260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9283463506156	20.318460663930683	13.528598043628978	23.22459494182474
2	15.503171643631555	21.330314599425847	39.61243139614799	23.554082360794602
3	17.39506138360771	27.597242050030147	31.41299758671164	23.59469897965051
4	12.05002004218716	18.238850301289926	39.46825777538294	30.242871881139973
5	12.154378883671855	38.89481443012636	35.38099221498923	13.569814471212553
6	28.39218567434965	39.478109610322015	18.20708567553252	13.92261903979581
7	24.717133819844218	34.42649882030256	23.543646176850462	17.312721183002765
8	26.020475886948923	33.91614480315944	21.048896365464355	19.014482944427286
9	26.790654426694875	14.195158333278574	21.234040176371245	37.7801470636553
10-11	24.244672392380025	26.220257065692376	28.73890287096117	20.79616767096643
12-13	25.69380466424408	24.67994927026725	28.428117545784897	21.198128519703772
14-15	22.81717957424802	25.154953238448346	27.1075225733384	24.920344613965234
16-17	21.104258800491525	27.945478679712703	28.012521438569056	22.937741081226715
18-19	20.96830048824082	26.554731598972264	31.180057695214185	21.296910217572727
20-21	22.354644859014712	25.732426944584997	30.703875041891454	21.209053154508837
22-23	22.355996222851747	25.650486961675877	30.425905007481358	21.56761180799102
24-25	21.85378731625257	25.926524027625362	30.825064562127498	21.394624093994572
26-27	21.604442794340876	26.17462001984505	30.381836521464855	21.83910066434922
28-29	21.346766864525897	26.459630737244126	30.536871405957065	21.65673099227291
30-31	21.82710045237897	25.940796953846895	30.48045613441949	21.751646459354646
32-33	21.95472594911536	26.318735324408504	29.938986995863193	21.787551730612943
34-35	22.201975331357566	26.250451776549678	30.353174264179213	21.19439862791355
36-37	22.24630953951405	25.841823537693486	30.060982932303048	21.850883990489418
38-39	22.042275814789946	25.830125604534558	30.297557745278926	21.83004083539657
40-41	21.93750072900319	25.58351879448254	30.494708545757334	21.984271930756936
42-43	22.121523359299612	25.62636291740963	29.87945378492247	22.372659938368287
44-45	22.121224168475948	26.271221802831413	29.68535613464197	21.922197894050665
46-47	22.494573813549465	26.122528974159987	28.87394885092329	22.508948361367263
48-49	22.765480052648837	26.24648356103802	28.540456872572335	22.447579513740806
50-51	22.650965530814418	26.529911199355755	28.10729149656701	22.711831773262816
52-53	22.356595973277848	26.81722039366955	28.191622098262144	22.63456153479046
54-55	22.263126750225148	26.805670507702057	28.064485498287027	22.866717243785768
56-57	22.870795129641202	26.48328237317294	27.865252267579283	22.780670229606574
58-59	22.290438538862027	26.41474655286365	28.455321817332617	22.83949309094171
60-61	22.32891695870771	26.923511642104113	28.069535886384262	22.678035512803916
62-63	22.51632999628231	26.615006692008734	27.919352875280246	22.94931043642871
64-65	21.94589820493072	26.678326687362492	28.123151767337575	23.25262334036921
66-67	22.325691964282047	26.594819605543762	27.987841451928574	23.091646978245617
68-69	22.25048123468136	26.42178418836241	28.185410518606453	23.14232405834978
70-71	22.319442949927428	26.297862763088613	27.989696183628727	23.39299810335523
72-73	22.616992061267656	26.311274143376906	27.951444963631534	23.12028883172391
74-75	22.572629595756297	26.84804056943489	27.63932395008532	22.94000588472349
76-77	22.579921241085408	26.637253919463504	27.525961363117368	23.25686347633372
78-79	22.360424871154088	26.78890955914098	27.611285870024492	23.23937969968044
80-81	22.43255759626374	26.79010907719318	27.65977614837271	23.117557178170365
82-83	22.81810185843828	26.352643616376387	28.067582978843642	22.761671546341688
84-85	22.852671616651254	26.760620762697595	27.29781735545839	23.088890265192767
86-87	22.34072123641279	27.031596682721233	27.469239339606432	23.15844274125955
88-89	22.651877701078877	26.495646321242894	27.381693012051123	23.470782965627105
90-91	22.46095428097081	26.458500591162476	27.60700024428473	23.47354488358198
92-93	22.806891883899883	26.496132843559227	27.51442380354714	23.18255146899375
94-95	22.006440114345622	26.435632780415297	27.919576275237755	23.638350830001322
96-97	22.426009091636406	26.217942009809875	27.681373152188392	23.674675746365324
98-99	22.116153104234336	26.716620221612153	27.404383780115428	23.762842894038087
100	22.27359841080996	26.251534796552594	27.340751612501528	24.13411518013591
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	709.0
1	475.5
2	606.0
3	885.5
4	515.5
5	387.5
6	844.0
7	2149.0
8	4074.0
9	4866.5
10	3331.0
11	1755.0
12	1693.0
13	1945.5
14	2242.5
15	2583.0
16	3010.5
17	3523.0
18	4036.5
19	4575.5
20	5134.0
21	5813.5
22	6945.5
23	8286.0
24	9977.5
25	12293.5
26	15919.5
27	20125.5
28	24665.0
29	31104.5
30	38038.5
31	44855.5
32	54334.0
33	61295.0
34	65271.0
35	69292.0
36	73952.0
37	83238.5
38	87024.0
39	86480.5
40	86348.0
41	83973.5
42	83899.0
43	87451.5
44	93182.5
45	104434.5
46	114687.0
47	121043.0
48	124479.5
49	120856.0
50	117758.5
51	117978.5
52	117321.5
53	115762.5
54	112457.0
55	102551.5
56	91045.5
57	77537.5
58	65885.5
59	60048.5
60	48778.0
61	36292.5
62	29565.0
63	23462.5
64	16103.5
65	10384.0
66	7318.0
67	5408.0
68	4072.0
69	3075.5
70	2443.5
71	1960.5
72	1660.0
73	1599.5
74	1327.0
75	1005.0
76	751.5
77	533.0
78	382.0
79	252.0
80	183.0
81	127.5
82	96.0
83	70.0
84	47.0
85	33.0
86	18.0
87	12.5
88	10.5
89	5.0
90	1.0
91	1.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006242648460037193
2	0.08444003443313466
3	4.928406678976732E-4
4	0.0
5	0.03926297320918129
6	0.0047312704118176625
7	9.856813357953462E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6428022263255773E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.599846233711616E-4
24-25	0.0
26-27	0.0
28-29	6.571208905302309E-5
30-31	1.149961558427904E-4
32-33	0.0015935181595358098
34-35	0.0013142417810604616
36-37	7.721170463730213E-4
38-39	6.40692868266975E-4
40-41	0.0013306698033237175
42-43	5.585527569506963E-4
44-45	3.942725343181385E-4
46-47	1.9713626715906924E-4
48-49	5.914088014772078E-4
50-51	6.899769350567423E-4
52-53	0.0012321016697441828
54-55	0.0014128099146399962
56-57	8.542571576893001E-4
58-59	0.0024806313617516213
60-61	0.0019056505825376694
62-63	8.049730908995328E-4
64-65	0.004336997877499523
66-67	0.004090577543550687
68-69	0.0034991687420734795
70-71	0.0015606621150092983
72-73	0.0048298385453971965
74-75	0.0034991687420734795
76-77	0.003778445120548827
78-79	7.228329795832539E-4
80-81	4.928406678976731E-5
82-83	9.856813357953462E-5
84-85	4.928406678976731E-5
86-87	3.2856044526511545E-5
88-89	1.4785220036930195E-4
90-91	1.6428022263255773E-5
92-93	0.0
94-95	1.6428022263255773E-5
96-97	4.4355660110790575E-4
98-99	6.40692868266975E-4
100	5.585527569506963E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3043580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.984229276491675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.23770487773447	31.121275127240672
2	16.124961904477818	15.152378143893594
3	6.813664621399665	9.60404342354885
4	3.5373838061534837	6.6480500714905615
5	2.0164210002033456	4.736999329574332
6	1.2976340231283146	3.658100067578221
7	0.8573875540868167	2.819858539401778
8	0.6008700800047129	2.258513408346026
9	0.4695324620694153	1.9854558765562536
>10	1.9598912194261613	15.044637443461143
>50	0.05651034206880184	1.7855670064836515
>100	0.025150483169892768	2.039884890226317
>500	0.001564130791824718	0.5570502564406687
>1k	9.62542025738288E-4	0.7329162344990126
>5k	1.20317753217286E-4	0.400517664591246
>10k+	2.40635506434572E-4	1.4547525166677662
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13290	0.4366568317573384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12444	0.40886061808790963	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7085	0.23278507547033425	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	3114	0.10231372265555694	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	4.928406678976732E-5	0.0
18-19	0.0	0.0	0.0	1.3142417810604615E-4	0.0
20-21	0.0	0.0	0.0	2.628483562120923E-4	0.0
22-23	0.0	0.0	0.0	6.242648460037193E-4	0.0
24-25	0.0	0.0	0.0	0.0015113780482195309	0.0
26-27	0.0	0.0	0.0	0.0029570440073860386	0.0
28-29	0.0	0.0	0.0	0.00905184026705393	0.0
30-31	0.0	0.0	0.0	0.02349207183645575	0.0
32-33	0.0	0.0	0.0	0.05023689208103615	0.0
34-35	0.0	0.0	0.0	0.08953272133474396	0.0
36-37	0.0	0.0	0.0	0.14974142292957635	0.0
38-39	0.0	0.0	0.0	0.23388575296197242	0.0
40-41	0.0	0.0	0.0	0.3276076199738466	0.0
42-43	0.0	0.0	0.0	0.4168282088855887	0.0
44-45	0.0	0.0	0.0	0.5229696607284842	0.0
46-47	0.0	0.0	0.0	0.6472969332168039	0.0
48-49	0.0	0.0	0.0	0.7785732591224808	0.0
50-51	0.0	0.0	0.0	0.9170450587794636	0.0
52-53	0.0	0.0	0.0	1.0513441407815796	0.0
54-55	0.0	0.0	0.0	1.1862839156519627	0.0
56-57	0.0	0.0	0.0	1.3427608277094736	0.0
58-59	0.0	0.0	0.0	1.519920619796424	0.0
60-61	0.0	0.0	0.0	1.7049165785029472	0.0
62-63	0.0	0.0	0.0	1.8839163090833821	0.0
64-65	0.0	0.0	0.0	2.0644438457342997	0.0
66-67	0.0	0.0	0.0	2.2634857634759067	0.0
68-69	0.0	0.0	0.0	2.4827012925567917	0.0
70-71	0.0	0.0	0.0	2.716357053207078	0.0
72-73	0.0	0.0	0.0	2.949339264944572	0.0
74-75	0.0	0.0	0.0	3.1786087436505692	0.0
76-77	0.0	0.0	0.0	3.4217763291912813	0.0
78-79	0.0	0.0	0.0	3.688370274479396	0.0
80-81	0.0	0.0	0.0	3.976468500910112	0.0
82-83	0.0	0.0	0.0	4.280649761136556	0.0
84-85	0.0	0.0	0.0	4.575614900873314	0.0
86-87	0.0	0.0	0.0	4.879467600654492	0.0
88	0.0	0.0	0.0	5.1138461942843625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7685	0.0	60.31696	1
GTATCAA	14525	0.0	42.961014	1
CAACGCA	18110	0.0	33.87912	5
ATCAACG	18180	0.0	33.749226	3
TATCAAC	18710	0.0	33.249363	2
AACGCAG	19545	0.0	31.741571	6
TCAACGC	19540	0.0	31.423796	4
GTACTAG	1370	0.0	29.540985	1
ACGCAGA	21395	0.0	28.665522	7
CGCAGAG	21765	0.0	28.13503	8
GCAGAGT	23400	0.0	25.86793	9
GTACATG	13240	0.0	25.697887	1
TACATGG	13125	0.0	25.31227	2
ACATGGG	13110	0.0	24.350859	3
TAGGCAT	2110	0.0	23.841928	5
GAGTACT	15690	0.0	23.67776	12-13
CAGAGTA	22595	0.0	23.160051	10-11
CATGGGG	8945	0.0	22.91639	4
GTGGTAT	2305	0.0	22.86621	1
AGAGTAC	21805	0.0	22.253363	10-11
>>END_MODULE