FastQCFastQC Report
Mon 16 Jan 2017
ERR1146504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531823
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.039.3636631
GTATCAA51750.035.339451
CAACGCA54200.031.7325525
ATCAACG55250.031.2995953
TATCAAC58900.030.5567172
AACGCAG62100.028.6015176
TCAACGC61300.028.440464
TCTAACG4450.026.3999582
ACGCAGA65900.025.7386027
GTACATG88550.025.69671
GTACTAG10650.025.1620371
TACATGG86000.024.9166472
CGCAGAG67550.024.8331478
ACATGGG84550.024.3435383
CTAACGC4750.023.7432863
TAACGCC5250.022.3771044
GCAGAGT76750.021.3666259
CATGGGG68250.020.3803794
TAGTACT13900.018.9319974
GCATCGT11500.018.7952848
Produced by FastQC (version 0.11.5)