##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146504_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2531823
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456088360047286	31.0	31.0	34.0	28.0	34.0
2	31.489596626620422	33.0	31.0	34.0	28.0	34.0
3	31.64954501163786	33.0	31.0	34.0	28.0	34.0
4	35.176286019994286	37.0	35.0	37.0	32.0	37.0
5	35.15028143752545	37.0	35.0	37.0	32.0	37.0
6	35.279988766987266	37.0	35.0	37.0	33.0	37.0
7	35.16931120382428	37.0	35.0	37.0	32.0	37.0
8	35.15017597991645	37.0	35.0	37.0	32.0	37.0
9	36.77095120788459	39.0	37.0	39.0	33.0	39.0
10-11	36.7476036042014	39.0	37.0	39.0	33.0	39.0
12-13	36.70575332477823	39.0	37.0	39.0	33.0	39.0
14-15	38.11588270586056	40.0	38.0	41.0	33.0	41.0
16-17	38.131200522311396	40.0	38.0	41.0	33.0	41.0
18-19	38.162265687609285	40.0	38.5	41.0	33.0	41.0
20-21	38.27294976781552	40.0	39.0	41.0	33.0	41.0
22-23	38.30353859649746	40.0	39.0	41.0	33.0	41.0
24-25	38.36178338691133	40.5	38.5	41.0	33.0	41.0
26-27	38.400658932318734	40.0	38.5	41.0	33.0	41.0
28-29	38.36844360762976	40.0	38.0	41.0	33.0	41.0
30-31	38.3525704600993	40.0	38.0	41.0	33.0	41.0
32-33	38.360556405404324	40.0	38.0	41.0	33.0	41.0
34-35	38.29873020349369	40.0	38.0	41.0	33.0	41.0
36-37	38.203517781456284	40.0	38.0	41.0	33.0	41.0
38-39	38.156344657584675	40.0	38.0	41.0	33.0	41.0
40-41	38.090034729915956	40.0	38.0	41.0	33.0	41.0
42-43	37.95387947735683	40.0	38.0	41.0	32.0	41.0
44-45	37.80274371470675	40.0	37.5	41.0	32.0	41.0
46-47	37.69425686550758	40.0	37.0	41.0	32.0	41.0
48-49	37.57102747703927	40.0	37.0	41.0	31.5	41.0
50-51	37.442700773316304	40.0	36.5	41.0	31.0	41.0
52-53	37.315192649723144	40.0	36.0	41.0	31.0	41.0
54-55	37.127489164921876	39.5	36.0	41.0	31.0	41.0
56-57	36.98873400707711	39.0	35.0	41.0	31.0	41.0
58-59	36.766692023889505	39.0	35.0	41.0	30.0	41.0
60-61	36.58157007816107	39.0	35.0	41.0	31.0	41.0
62-63	36.34592821062136	38.0	35.0	40.0	30.0	41.0
64-65	36.0922596089853	37.5	35.0	40.0	30.0	41.0
66-67	35.82634311324291	37.0	35.0	40.0	30.0	41.0
68-69	35.530940551531444	36.5	35.0	39.5	30.0	41.0
70-71	35.26459807814369	36.0	34.5	39.0	30.0	41.0
72-73	34.9487312106731	35.5	34.0	39.0	29.0	41.0
74-75	34.635710908700965	35.0	34.0	37.5	29.0	40.0
76-77	34.331081398660174	35.0	34.0	37.0	29.0	39.0
78-79	34.0426479260201	35.0	34.0	37.0	29.0	39.0
80-81	33.76453883229594	35.0	34.0	36.0	29.0	39.0
82-83	33.50823023568393	35.0	34.0	36.0	28.0	37.0
84-85	33.28324728861378	35.0	34.0	35.5	28.0	37.0
86-87	33.09150027470325	35.0	34.0	35.0	28.0	37.0
88-89	32.941794311845655	35.0	33.5	35.0	27.5	36.0
90-91	32.774367915924614	35.0	33.0	35.0	27.0	36.0
92-93	32.677235730933795	35.0	33.0	35.0	27.0	36.0
94-95	32.570673976814334	35.0	33.0	35.0	27.0	36.0
96-97	32.48736878525868	35.0	33.0	35.0	27.0	35.0
98-99	32.40518413017024	35.0	33.0	35.0	26.0	35.0
100	31.47712458572341	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3051507927394752
1101	2	0.34966626121773103
1101	3	0.38735258087889335
1101	4	0.36718032085187957
1101	5	0.2908488029931888
1101	6	0.3059367370218524
1101	7	0.2810278501416903
1101	8	0.31256901397021153
1101	9	0.41294102385217
1101	10-11	0.4326836065815982
1101	12-13	0.38420937554742096
1101	14-15	0.53494135005419
1101	16-17	0.4810839553834185
1101	18-19	0.45522469871553284
1101	20-21	0.5463251949853571
1101	22-23	0.4034753208870967
1101	24-25	0.394737678997231
1101	26-27	0.4513748148890784
1101	28-29	0.4417605516934273
1101	30-31	0.4879650386404961
1101	32-33	0.4515894338563484
1101	34-35	0.5394003697663905
1101	36-37	0.49259419122037684
1101	38-39	0.46233691711253044
1101	40-41	0.4717256708864852
1101	42-43	0.4128647504622407
1101	44-45	0.3050027047095014
1101	46-47	0.29632162701046383
1101	48-49	0.3216227683670354
1101	50-51	0.3911228997089182
1101	52-53	0.42549858198722035
1101	54-55	0.4282312999947635
1101	56-57	0.35987344312075464
1101	58-59	0.3783109110934433
1101	60-61	0.3596088509791926
1101	62-63	0.31709937721304726
1101	64-65	0.36143082179524555
1101	66-67	0.3283910425924432
1101	68-69	0.3003711238108622
1101	70-71	0.29038109959863334
1101	72-73	0.3592907459541195
1101	74-75	0.31185593563345293
1101	76-77	0.3197711317907945
1101	78-79	0.3853239064421956
1101	80-81	0.41004425356972973
1101	82-83	0.4226975170959619
1101	84-85	0.4009306898067777
1101	86-87	0.39717729647807687
1101	88-89	0.35897367866638064
1101	90-91	0.44336890630424364
1101	92-93	0.4204041684673214
1101	94-95	0.39920235514710356
1101	96-97	0.3098334467079553
1101	98-99	0.31917236563394624
1101	100	0.3269048563459158
1102	1	0.26347368873632604
1102	2	0.2574418898367412
1102	3	0.24577551638698125
1102	4	0.239429706083655
1102	5	0.16680358338599888
1102	6	0.19284590348970454
1102	7	0.2707954529538412
1102	8	0.19213085839208333
1102	9	0.2799309847402185
1102	10-11	0.2762907222593398
1102	12-13	0.3672953779419359
1102	14-15	0.49091457895654855
1102	16-17	0.4290447862199045
1102	18-19	0.35803842915832007
1102	20-21	0.3117576205402486
1102	22-23	0.2891555965162169
1102	24-25	0.33779888804107117
1102	26-27	0.4133222063429258
1102	28-29	0.4412480379146473
1102	30-31	0.4894740242187936
1102	32-33	0.394585436447521
1102	34-35	0.4228334810567702
1102	36-37	0.4163582632496272
1102	38-39	0.4138000744238681
1102	40-41	0.3867718438090293
1102	42-43	0.28897343397525077
1102	44-45	0.3432171126637158
1102	46-47	0.32382116962171636
1102	48-49	0.33507595708672255
1102	50-51	0.349051328947553
1102	52-53	0.35123051821064166
1102	54-55	0.299336617404677
1102	56-57	0.27747106060709115
1102	58-59	0.410802496828957
1102	60-61	0.3256603753004512
1102	62-63	0.3056781169098812
1102	64-65	0.3223859130733544
1102	66-67	0.2800105364711811
1102	68-69	0.32707204877904417
1102	70-71	0.23957275809917888
1102	72-73	0.24413158162174398
1102	74-75	0.2040295423260048
1102	76-77	0.25753661343157574
1102	78-79	0.19969533908307824
1102	80-81	0.23882219028858032
1102	82-83	0.2330168628423266
1102	84-85	0.2496136783818912
1102	86-87	0.2266269142878059
1102	88-89	0.24301271831091498
1102	90-91	0.20448330394858516
1102	92-93	0.19669816146813446
1102	94-95	0.24312189564869868
1102	96-97	0.21118174531119394
1102	98-99	0.15846378752397072
1102	100	0.07113435842686044
1103	1	0.23772573367939742
1103	2	0.270672374887873
1103	3	0.3145200602677285
1103	4	0.3417301177825607
1103	5	0.36662785038492984
1103	6	0.3504675263816921
1103	7	0.37496065865356343
1103	8	0.42695635544620814
1103	9	0.40821484357309856
1103	10-11	0.48482615865221845
1103	12-13	0.5165304503976884
1103	14-15	0.6122750181071055
1103	16-17	0.5594133422184981
1103	18-19	0.5588278346114279
1103	20-21	0.4788077870810099
1103	22-23	0.47037366767595046
1103	24-25	0.46198049384496187
1103	26-27	0.5023805242263393
1103	28-29	0.5589762605360704
1103	30-31	0.4789515559055104
1103	32-33	0.42334010885671347
1103	34-35	0.4121614255227186
1103	36-37	0.4793263807496544
1103	38-39	0.4016561697169436
1103	40-41	0.39543473021977604
1103	42-43	0.40182607033037243
1103	44-45	0.3840676437101962
1103	46-47	0.3898559909869945
1103	48-49	0.330360382237771
1103	50-51	0.41242731682970657
1103	52-53	0.45804531776471435
1103	54-55	0.4927633180554878
1103	56-57	0.3932158903003611
1103	58-59	0.4113773912697454
1103	60-61	0.2819600584427846
1103	62-63	0.31305555773550253
1103	64-65	0.20290092903375267
1103	66-67	0.22853632763926868
1103	68-69	0.26770269561370696
1103	70-71	0.23216707717739382
1103	72-73	0.20554395411960513
1103	74-75	0.28798753774163544
1103	76-77	0.3313100266509039
1103	78-79	0.24632466564586508
1103	80-81	0.2796702574340486
1103	82-83	0.23484858371039508
1103	84-85	0.21399817885249917
1103	86-87	0.23179091912008687
1103	88-89	0.18361725727144318
1103	90-91	0.26921841753252806
1103	92-93	0.2565769261667228
1103	94-95	0.2653888797921695
1103	96-97	0.19155449941804648
1103	98-99	0.23173646024626748
1103	100	0.18793113170210063
1104	1	0.17037818593536969
1104	2	0.15500181179870864
1104	3	0.18645995057731213
1104	4	0.201414184478395
1104	5	0.15780138185607484
1104	6	0.2495742031570387
1104	7	0.24550732538224906
1104	8	0.25912032075945035
1104	9	0.3356012935256345
1104	10-11	0.34620063596975825
1104	12-13	0.323399393283033
1104	14-15	0.40716215769457875
1104	16-17	0.3574360398843268
1104	18-19	0.30604071659852394
1104	20-21	0.3069429337040148
1104	22-23	0.30626984604290897
1104	24-25	0.29142988251176405
1104	26-27	0.31680803599127216
1104	28-29	0.28918033880989924
1104	30-31	0.24926546413379924
1104	32-33	0.26268736893978684
1104	34-35	0.324311192048647
1104	36-37	0.2858050671838299
1104	38-39	0.2965621557482123
1104	40-41	0.3169510540845124
1104	42-43	0.3018094153335369
1104	44-45	0.22821408317241065
1104	46-47	0.18838652294150648
1104	48-49	0.25272774781799257
1104	50-51	0.24149651181195964
1104	52-53	0.15200916944472453
1104	54-55	0.26205739842953335
1104	56-57	0.16001243672307908
1104	58-59	0.14464640936197526
1104	60-61	0.15530963054711577
1104	62-63	0.18687584370054822
1104	64-65	0.19390293729362895
1104	66-67	0.13774593265286939
1104	68-69	0.11940180046576643
1104	70-71	0.14430342668116225
1104	72-73	0.19359126575982089
1104	74-75	0.15812489584767775
1104	76-77	0.1623295646974583
1104	78-79	0.16803830918313167
1104	80-81	0.1391539632656631
1104	82-83	0.1258081762908887
1104	84-85	0.10761622020340411
1104	86-87	0.13525826226704396
1104	88-89	0.15154869593042264
1104	90-91	0.154722291116272
1104	92-93	0.21486116810676492
1104	94-95	0.054832017915579456
1104	96-97	0.05551251379894495
1104	98-99	0.07677660755444293
1104	100	0.16267931208555453
1105	1	0.06438819926007966
1105	2	0.09545516593852099
1105	3	0.12108929219224507
1105	4	0.1459480911634543
1105	5	0.190450430287747
1105	6	0.14531974262757785
1105	7	0.18009757633684842
1105	8	0.20026606718734286
1105	9	0.21157919019521643
1105	10-11	0.30320830625152695
1105	12-13	0.1978135395731755
1105	14-15	0.2012487159867291
1105	16-17	0.23785485870672574
1105	18-19	0.20868232788743768
1105	20-21	0.2284757057681901
1105	22-23	0.20726642647790072
1105	24-25	0.2829773329949319
1105	26-27	0.24462655317923065
1105	28-29	0.19693071924136518
1105	30-31	0.23897325690239057
1105	32-33	0.22918989615511265
1105	34-35	0.19258335263076276
1105	36-37	0.2687406303211546
1105	38-39	0.32360793405193533
1105	40-41	0.19143809247277943
1105	42-43	0.17775462626070038
1105	44-45	0.06452487689711006
1105	46-47	0.18574930831882597
1105	48-49	0.16756159556954486
1105	50-51	0.18174106562760528
1105	52-53	0.16078080784007653
1105	54-55	0.2551697149875167
1105	56-57	0.21745905462476145
1105	58-59	0.26373201518085665
1105	60-61	0.22111507955376197
1105	62-63	0.21508793689631744
1105	64-65	0.19727341171292068
1105	66-67	0.16686194571626345
1105	68-69	0.1803058757042919
1105	70-71	0.1534854343659049
1105	72-73	0.1497002227524007
1105	74-75	0.1829513907534519
1105	76-77	0.16004842218902837
1105	78-79	0.2004345493062374
1105	80-81	0.18228707721196002
1105	82-83	0.13673947036446776
1105	84-85	0.2155773926818867
1105	86-87	0.1923902953937784
1105	88-89	0.12737694892418716
1105	90-91	0.09943078346718437
1105	92-93	0.1568600496014767
1105	94-95	0.08352481053169214
1105	96-97	0.0810578057834519
1105	98-99	0.10206128136272241
1105	100	0.15166103509442408
1106	1	0.14142676428799916
1106	2	0.03134392254301588
1106	3	0.0064296108962444976
1106	4	0.03624707040423658
1106	5	0.0842736922040146
1106	6	0.1478906816086294
1106	7	-0.005099870503570969
1106	8	0.012905853004525625
1106	9	0.15411565034495567
1106	10-11	0.18436740052077738
1106	12-13	0.2147870895322299
1106	14-15	0.1767316542471704
1106	16-17	0.16150954880163937
1106	18-19	0.1383800097036172
1106	20-21	0.14101732760317987
1106	22-23	0.16376407801722337
1106	24-25	0.12851679041197173
1106	26-27	0.133389288499842
1106	28-29	0.25036807385527027
1106	30-31	0.22753513687823101
1106	32-33	0.16772658078746616
1106	34-35	0.20706271685450162
1106	36-37	0.15553171506027752
1106	38-39	0.19188757430440262
1106	40-41	0.2392250641339757
1106	42-43	0.13082019333985784
1106	44-45	0.12101149294672098
1106	46-47	0.09481310028758827
1106	48-49	0.1305211394171053
1106	50-51	0.18875025220118857
1106	52-53	0.13999352940170695
1106	54-55	0.20878104362511607
1106	56-57	0.12655833465338162
1106	58-59	0.11238345711613817
1106	60-61	0.1269830597266619
1106	62-63	0.125939926220191
1106	64-65	0.14934991123671892
1106	66-67	0.11536393693832281
1106	68-69	0.24235751472210154
1106	70-71	0.18623949880986146
1106	72-73	0.14753108878132082
1106	74-75	0.12888786641889993
1106	76-77	0.15506288033115112
1106	78-79	0.1752451008378486
1106	80-81	0.16111402823584342
1106	82-83	0.10563058993141539
1106	84-85	0.1867653324950851
1106	86-87	0.0759305087890283
1106	88-89	0.11638720461979091
1106	90-91	0.17265479471206646
1106	92-93	0.13797144131585526
1106	94-95	0.0897020909578714
1106	96-97	0.10719411341706575
1106	98-99	0.12761881528918195
1106	100	0.18887209331535715
1107	1	0.07501484731867691
1107	2	0.08166739927827749
1107	3	0.08947247419423476
1107	4	0.20303723529374906
1107	5	0.18325591178275147
1107	6	0.1250119435721686
1107	7	0.1623047949602352
1107	8	0.09743689757796403
1107	9	0.14691886063418735
1107	10-11	0.2140213728992606
1107	12-13	0.12742965244709836
1107	14-15	0.13686255128731517
1107	16-17	0.12124569457163403
1107	18-19	0.128487303630493
1107	20-21	0.15061817913174025
1107	22-23	0.13844406255502406
1107	24-25	0.13648081975470205
1107	26-27	0.11566037097561122
1107	28-29	0.14920530762799444
1107	30-31	0.13290603582345284
1107	32-33	0.10963678262675813
1107	34-35	0.1090276123462175
1107	36-37	0.07838010465452072
1107	38-39	0.1198010524463271
1107	40-41	0.06928165179500922
1107	42-43	0.13193045897733668
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2216	26-27	-0.3400734883821528
2216	28-29	-0.3276068224614903
2216	30-31	-0.38147449555960833
2216	32-33	-0.42900562047542934
2216	34-35	-0.38711493688857246
2216	36-37	-0.33783609011628357
2216	38-39	-0.34378379028458994
2216	40-41	-0.31630814066497237
2216	42-43	-0.24707297229524983
2216	44-45	-0.2281106566327722
2216	46-47	-0.14351693940060528
2216	48-49	-0.18981750832077893
2216	50-51	-0.1688043985824521
2216	52-53	-0.12772550353734857
2216	54-55	-0.1651393719195653
2216	56-57	-0.13085574445180015
2216	58-59	-0.0918237599782401
2216	60-61	-0.13779691331622246
2216	62-63	-0.19713222274193498
2216	64-65	-0.259478740978885
2216	66-67	-0.1378837654970937
2216	68-69	-0.1754385895686994
2216	70-71	-0.24852116196119312
2216	72-73	-0.17050400065150484
2216	74-75	-0.19769085048192636
2216	76-77	-0.2232569060627938
2216	78-79	-0.21392502738148522
2216	80-81	-0.18964625092971943
2216	82-83	-0.19012631364238786
2216	84-85	-0.1838426400142552
2216	86-87	-0.21282873463767515
2216	88-89	-0.20707396931983624
2216	90-91	-0.21903843124239586
2216	92-93	-0.21760854503781957
2216	94-95	-0.22092316896657138
2216	96-97	-0.14085598608994587
2216	98-99	-0.18795801390476186
2216	100	-0.2568940541696243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	68.0
1	39.0
2	27.0
3	34.0
4	38.0
5	40.0
6	113.0
7	710.0
8	2058.0
9	2507.0
10	2542.0
11	2882.0
12	3138.0
13	3688.0
14	4717.0
15	5424.0
16	5876.0
17	6303.0
18	6967.0
19	7854.0
20	9095.0
21	10096.0
22	11174.0
23	13216.0
24	15933.0
25	19808.0
26	23109.0
27	21957.0
28	22727.0
29	28959.0
30	33848.0
31	36727.0
32	46970.0
33	64126.0
34	96587.0
35	150289.0
36	285507.0
37	617063.0
38	740953.0
39	228357.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65111961165034	19.80202324075	13.174619422146886	24.372237725452784
2	15.270262103105553	20.99542535218389	39.63712507816324	24.09718746654732
3	18.009180255340667	26.34587125521426	30.29346005561876	25.35148843382632
4	12.375472237715499	17.684443292762893	39.08797091442287	30.852113555098747
5	13.19058282767194	38.49124413441343	35.010150096602686	13.30802294131194
6	29.93511387660813	38.176354684473154	18.5557089992068	13.332822439711919
7	26.213696539427882	33.119238913494186	23.184644291357476	17.482420255720452
8	25.804405289127715	34.776654590383316	21.040975097998015	18.377965022490958
9	26.486011845276703	15.636926548066432	20.694197678818725	37.18286392783814
10-11	24.36603904382234	26.47247784787939	28.783250399691106	20.378232708607158
12-13	25.02695365354083	24.66948076173721	28.009047816640503	22.294517768081462
14-15	22.5937347672856	25.856211866368454	25.611955167529242	25.938098198816697
16-17	22.32899604060166	28.530953994386493	26.457577832583436	22.682472132428412
18-19	22.207710231736716	28.235661470922015	27.687060830147303	21.86956746719397
20-21	23.39089385832345	27.493330447209612	26.956467984961606	22.159307709505327
22-23	23.4444254633392	27.17724742594818	26.70218217977038	22.67614493094224
24-25	23.050226524945394	27.41060460633638	27.07241688489689	22.46675198382134
26-27	22.86322798706086	27.508350619159426	26.69518112050072	22.933240273278987
28-29	22.669655214910037	27.909252570641286	26.708461312401642	22.71263090204703
30-31	23.010162001083906	27.494718729903916	26.69196172520963	22.803157543802545
32-33	22.864478819384097	27.859620155161245	26.26331194994391	23.012589075510753
34-35	23.214046234177772	27.740723923183868	26.66870232987975	22.376527512758614
36-37	23.245196899136612	27.310285243956855	26.575067666208614	22.869450190697922
38-39	23.00651250325171	27.384097451114748	26.732787869294842	22.8766021763387
40-41	22.95596366227852	27.126343741950983	27.011069921196633	22.906622674573864
42-43	22.81782720715108	27.29173551580407	26.732810563849497	23.157626713195352
44-45	22.76485415489647	27.540762748359416	26.922101370544937	22.77228172619918
46-47	23.019798850756366	27.480940761484536	26.500480431882977	22.998779955876124
48-49	22.962821898448432	27.401902672026036	26.567219256904185	23.068056172621347
50-51	22.914664829190585	27.37073529597694	26.576664317141628	23.13793555769085
52-53	22.952751736824474	27.50148554595394	26.470690494402337	23.07507222281925
54-55	22.681639274112626	27.43044586910881	26.615139307796454	23.272775548982104
56-57	23.10665486817277	27.354270720907337	26.424378707156126	23.11469570376376
58-59	22.807150289591746	27.21038306477503	26.815166440428772	23.16730020520445
60-61	22.720214439754805	27.485465495319005	26.702791209676974	23.091528855249212
62-63	22.889872081372303	27.18744962433583	26.612100274043616	23.31057802024825
64-65	22.536894939876998	27.27822290286619	26.70468026360993	23.480201893646875
66-67	22.870691533697148	27.259154888952413	26.463560322726394	23.406593254624045
68-69	22.79532453041023	27.063891393560834	26.754644531074046	23.386139544954887
70-71	22.796814548336833	26.95465913497945	26.671687440249077	23.57683887643464
72-73	22.96715599494808	27.080642857523856	26.755681781139927	23.19651936638813
74-75	22.858189936187447	27.53280518402908	26.623999841950337	22.98500503783314
76-77	22.990099408935862	27.139283539699367	26.573704817218186	23.29691223414659
78-79	22.92869470526597	27.182609925670477	26.577961446004018	23.310733923059537
80-81	22.914708680271893	27.23476178669119	26.753148995314664	23.097380537722252
82-83	23.103378039454153	26.866977904559374	27.110590603239253	22.919053452747217
84-85	23.148852575346694	27.275088943857256	26.611899363499997	22.964159117296052
86-87	22.88677100921556	27.35968388660487	26.65326084787754	23.10028425630203
88-89	23.145526448821506	26.9477451735911	26.561798400939374	23.344929976648018
90-91	23.046779774056525	26.91610031975668	26.7362495342726	23.300870371914197
92-93	23.27865458390962	26.975135073625967	26.723054271060747	23.023156071403665
94-95	22.763548703578362	26.901682477553994	27.01528063090241	23.319488187965234
96-97	22.98778115966279	26.772024227089354	26.902843713779923	23.337350899467932
98-99	22.827333006063707	27.134056421415288	26.646273225360694	23.39233734716031
100	23.03922614433622	26.70610378496775	26.6941722985653	23.560497772130734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1153.0
1	857.5
2	612.5
3	671.0
4	656.0
5	566.0
6	507.5
7	526.0
8	557.0
9	562.0
10	470.0
11	410.5
12	399.0
13	393.5
14	452.0
15	482.0
16	539.5
17	683.0
18	799.5
19	991.0
20	1370.5
21	1874.5
22	2695.0
23	3819.0
24	5151.5
25	7252.0
26	10267.5
27	13904.0
28	17888.0
29	22781.5
30	28149.5
31	33663.5
32	41142.5
33	46595.0
34	50774.5
35	55318.5
36	60073.0
37	67261.5
38	71078.5
39	71354.5
40	72377.0
41	73915.0
42	75122.5
43	78287.0
44	82842.5
45	90942.5
46	97560.5
47	100433.0
48	103154.5
49	101814.0
50	99036.5
51	99985.5
52	99760.0
53	96449.5
54	93025.5
55	85792.5
56	78440.0
57	69434.0
58	60659.5
59	54583.5
60	45303.0
61	36244.0
62	29324.5
63	22178.5
64	15707.5
65	11124.5
66	8174.0
67	5997.0
68	4359.5
69	3243.5
70	2567.5
71	2023.0
72	1558.5
73	1391.0
74	1190.0
75	921.5
76	701.5
77	499.5
78	367.5
79	290.0
80	216.5
81	151.0
82	115.5
83	95.5
84	75.0
85	62.0
86	37.0
87	22.5
88	22.5
89	21.5
90	16.5
91	13.5
92	13.0
93	10.0
94	8.0
95	6.5
96	6.5
97	6.5
98	5.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06027277578250928
2	0.010348274741164764
3	0.011967661246461541
4	0.010229783045655246
5	0.012639114187682158
6	0.012283639101153595
7	0.002922795155901499
8	0.0054901152252744365
9	0.007148998962407719
10-11	0.007129250346489466
12-13	0.006418300173432345
14-15	0.010466766436674286
16-17	0.009933553806881445
18-19	0.007899446367301347
20-21	0.0066750321803696385
22-23	0.002587068685291191
24-25	0.0036732425607951267
26-27	0.004917405363645089
28-29	0.006280059862004571
30-31	0.009281849481579084
32-33	0.010387771973001272
34-35	0.011750426471360756
36-37	0.018306966956220874
38-39	0.01822797249254786
40-41	0.018129229412956593
42-43	0.02466602128189846
44-45	0.028161526299429303
46-47	0.030294376818600668
48-49	0.03173602578063316
50-51	0.03219024394675299
52-53	0.030294376818600668
54-55	0.03691016315121555
56-57	0.03916150536589643
58-59	0.036831168687542534
60-61	0.03748287301284489
62-63	0.033138177510829155
64-65	0.03501429602306322
66-67	0.03746312439692664
68-69	0.03937874014099722
70-71	0.03967496937977102
72-73	0.035725246196120344
74-75	0.03843080657692106
76-77	0.038075331490392494
78-79	0.03677192283978777
80-81	0.04168932820343286
82-83	0.03797658841080123
84-85	0.03446133477735213
86-87	0.032881445503891854
88-89	0.03059060605737447
90-91	0.03301968581531963
92-93	0.03509329048673624
94-95	0.03839130934508455
96-97	0.033750384604295004
98-99	0.030827589448393512
100	0.02784554844473725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2531823.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.51936508176815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.258263248453	39.70952430550289
2	15.01916894487622	16.97747785640427
3	6.16252733752337	10.449063972475798
4	3.119318096519269	7.052075132133546
5	1.7383043430438723	4.912392889385988
6	1.0349830723501119	3.509795167176361
7	0.7198751579049406	2.8480820804047386
8	0.46846751980978646	2.1181989424863814
9	0.3166424600832053	1.610678772164869
>10	1.1387204825173647	9.73858776535493
>50	0.0198907707883984	0.7412087823406915
>100	0.0038385661304986786	0.3329143341695517
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	7.899446367301347E-5
14-15	0.0	0.0	0.0	0.0	7.899446367301347E-5
16-17	0.0	0.0	0.0	1.974861591825337E-5	7.899446367301347E-5
18-19	0.0	0.0	0.0	3.949723183650674E-5	9.874307959126685E-5
20-21	0.0	0.0	0.0	1.3824031142777358E-4	1.974861591825337E-4
22-23	0.0	0.0	0.0	4.344695502015741E-4	2.3698339101904043E-4
24-25	0.0	0.0	0.0	0.0011059224914221886	2.3698339101904043E-4
26-27	0.0	0.0	0.0	0.0025475714534546846	2.7648062285554715E-4
28-29	0.0	0.0	0.0	0.00837341314933943	2.7648062285554715E-4
30-31	0.0	0.0	0.0	0.023105880624356444	2.7648062285554715E-4
32-33	0.0	0.0	0.0	0.05071444567807465	2.7648062285554715E-4
34-35	0.0	0.0	0.0	0.0964719887606677	3.159778546920539E-4
36-37	0.0	0.0	0.0	0.16486144568557914	3.159778546920539E-4
38-39	0.0	0.0	0.0	0.25580382198913587	3.159778546920539E-4
40-41	0.0	0.0	0.0	0.35454690158040275	3.159778546920539E-4
42-43	0.0	0.0	0.0	0.44864905643088004	3.159778546920539E-4
44-45	0.0	0.0	0.0	0.5629342967498123	3.357264706103073E-4
46-47	0.0	0.0	0.0	0.6977383490078097	3.75223702446814E-4
48-49	0.0	0.0	0.0	0.8359589118196651	3.949723183650674E-4
50-51	0.0	0.0	0.0	0.981861686223721	3.949723183650674E-4
52-53	0.0	0.0	0.0	1.1219781161637288	4.344695502015741E-4
54-55	0.0	0.0	0.0	1.267920387799621	4.344695502015741E-4
56-57	0.0	1.974861591825337E-5	0.0	1.4354478966341644	4.344695502015741E-4
58-59	0.0	3.949723183650674E-5	0.0	1.6169179283069945	4.344695502015741E-4
60-61	0.0	3.949723183650674E-5	0.0	1.8046877684577476	4.344695502015741E-4
62-63	0.0	3.949723183650674E-5	0.0	1.991312188885242	4.344695502015741E-4
64-65	0.0	3.949723183650674E-5	0.0	2.181372868482512	4.344695502015741E-4
66-67	0.0	3.949723183650674E-5	0.0	2.3922288406417036	4.344695502015741E-4
68-69	0.0	3.949723183650674E-5	0.0	2.6242355804493442	4.344695502015741E-4
70-71	0.0	3.949723183650674E-5	0.0	2.8658796448250925	4.344695502015741E-4
72-73	0.0	3.949723183650674E-5	0.0	3.1114141865367366	4.344695502015741E-4
74-75	0.0	3.949723183650674E-5	0.0	3.3574819408781735	4.344695502015741E-4
76-77	0.0	3.949723183650674E-5	0.0	3.6189733642517665	4.344695502015741E-4
78-79	0.0	3.949723183650674E-5	0.0	3.907006927419492	4.344695502015741E-4
80-81	0.0	3.949723183650674E-5	0.0	4.206810665674496	4.344695502015741E-4
82-83	0.0	3.949723183650674E-5	0.0	4.516784941127401	4.344695502015741E-4
84-85	0.0	3.949723183650674E-5	0.0	4.831024917618649	4.344695502015741E-4
86-87	0.0	3.949723183650674E-5	0.0	5.146983023694784	4.5421816611982747E-4
88	0.0	3.949723183650674E-5	0.0	5.397612708313338	4.7396678203808087E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2950	0.0	39.363663	1
GTATCAA	5175	0.0	35.33945	1
CAACGCA	5420	0.0	31.732552	5
ATCAACG	5525	0.0	31.299595	3
TATCAAC	5890	0.0	30.556717	2
AACGCAG	6210	0.0	28.601517	6
TCAACGC	6130	0.0	28.44046	4
TCTAACG	445	0.0	26.399958	2
ACGCAGA	6590	0.0	25.738602	7
GTACATG	8855	0.0	25.6967	1
GTACTAG	1065	0.0	25.162037	1
TACATGG	8600	0.0	24.916647	2
CGCAGAG	6755	0.0	24.833147	8
ACATGGG	8455	0.0	24.343538	3
CTAACGC	475	0.0	23.743286	3
TAACGCC	525	0.0	22.377104	4
GCAGAGT	7675	0.0	21.366625	9
CATGGGG	6825	0.0	20.380379	4
TAGTACT	1390	0.0	18.931997	4
GCATCGT	1150	0.0	18.795284	8
>>END_MODULE