FastQCFastQC Report
Mon 16 Jan 2017
ERR1146504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531823
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102480.40476763186052106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.35500111974652254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49010.19357593323071953No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT26100.10308777509328258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54150.059.8745231
GTATCAA105050.043.6298941
CAACGCA132950.033.914945
TATCAAC134850.033.813142
ATCAACG133450.033.7526363
TCAACGC141300.032.0438654
AACGCAG142750.031.902766
ACGCAGA155650.029.0467787
CGCAGAG157850.028.6419498
GCAGAGT169400.026.3284239
GTGGTAT18750.024.594961
GTACATG83250.023.5142081
TACATGG83600.023.0775832
GAGTACT124750.022.66037612-13
ACATGGG83000.022.6024513
AGAGTAC160700.022.50426110-11
CAGAGTA168000.022.25373610-11
TGGTATC19450.021.5319392
GTACTAG11300.021.237971
GTACTTT132200.021.20562414-15
Produced by FastQC (version 0.11.5)