##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2531823
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.425747771467435	34.0	31.0	34.0	31.0	34.0
2	32.51176642285026	34.0	31.0	34.0	31.0	34.0
3	32.67880100623148	34.0	31.0	34.0	31.0	34.0
4	36.186840865257956	37.0	37.0	37.0	35.0	37.0
5	36.19334447945216	37.0	37.0	37.0	35.0	37.0
6	36.28054804779007	37.0	37.0	37.0	35.0	37.0
7	36.27187959031891	37.0	37.0	37.0	35.0	37.0
8	36.28019020286963	37.0	37.0	37.0	35.0	37.0
9	38.09170309298873	39.0	39.0	39.0	37.0	39.0
10-11	38.06154991877395	39.0	38.0	39.0	36.0	39.0
12-13	38.03531111772031	39.0	38.0	39.0	35.0	39.0
14-15	39.48501040554572	41.0	39.0	41.0	36.5	41.0
16-17	39.48079842074269	41.0	39.0	41.0	36.5	41.0
18-19	39.53932719625345	41.0	39.5	41.0	37.0	41.0
20-21	39.572329700772926	41.0	40.0	41.0	37.0	41.0
22-23	39.52953208024415	41.0	39.5	41.0	37.0	41.0
24-25	39.513560189634106	41.0	39.0	41.0	36.0	41.0
26-27	39.43364346559771	41.0	39.0	41.0	36.0	41.0
28-29	39.34044559987014	41.0	39.0	41.0	36.0	41.0
30-31	39.22038507431206	41.0	39.0	41.0	35.5	41.0
32-33	39.07854439271624	41.0	39.0	41.0	35.0	41.0
34-35	38.98455677983809	41.0	39.0	41.0	35.0	41.0
36-37	38.88539048740769	41.0	39.0	41.0	35.0	41.0
38-39	38.77356355479826	40.5	38.0	41.0	35.0	41.0
40-41	38.67441483863603	40.0	38.0	41.0	35.0	41.0
42-43	38.56164569956115	40.0	38.0	41.0	35.0	41.0
44-45	38.39753272641887	40.0	38.0	41.0	34.5	41.0
46-47	38.13793855257654	40.0	38.0	41.0	34.0	41.0
48-49	37.8851916188454	40.0	38.0	41.0	33.0	41.0
50-51	37.72106027949031	40.0	37.0	41.0	33.0	41.0
52-53	37.58294991395528	40.0	37.0	41.0	33.0	41.0
54-55	37.43159928636402	40.0	36.5	41.0	33.0	41.0
56-57	37.22771990775027	40.0	36.0	41.0	32.5	41.0
58-59	37.0096286351771	39.0	35.0	41.0	32.0	41.0
60-61	36.81934953588778	39.0	35.0	41.0	32.0	41.0
62-63	36.5550087822095	39.0	35.0	41.0	32.0	41.0
64-65	36.257978539574054	38.0	35.0	41.0	31.0	41.0
66-67	35.99618476489076	37.0	35.0	40.0	31.0	41.0
68-69	35.678619121478874	37.0	35.0	40.0	31.0	41.0
70-71	35.39032724641494	36.0	35.0	39.0	31.0	41.0
72-73	35.068923459499345	36.0	35.0	39.0	31.0	41.0
74-75	34.74956760405447	35.0	35.0	38.5	30.0	40.5
76-77	34.45379989043468	35.0	35.0	37.0	30.0	39.5
78-79	34.17240265216012	35.0	35.0	37.0	30.0	39.0
80-81	33.92552066238437	35.0	34.5	36.5	30.0	39.0
82-83	33.68347984831483	35.0	34.0	36.0	30.0	37.0
84-85	33.48503548628794	35.0	34.0	36.0	29.5	37.0
86-87	33.312678058458275	35.0	34.0	35.0	29.0	37.0
88-89	33.16944687681564	35.0	34.0	35.0	29.0	36.0
90-91	33.08821983211307	35.0	34.0	35.0	29.0	36.0
92-93	33.00919831283625	35.0	34.0	35.0	29.0	36.0
94-95	32.9398834357694	35.0	34.0	35.0	29.0	36.0
96-97	32.89363671947052	35.0	34.0	35.0	29.0	35.5
98-99	32.83954012583028	35.0	34.0	35.0	29.0	35.0
100	32.28895621850343	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22236375160426292
1101	2	0.2695732072543109
1101	3	0.29233665999299774
1101	4	0.22981161806460904
1101	5	0.18387527128180636
1101	6	0.19051838338975102
1101	7	0.19649327439980624
1101	8	0.1884110724714887
1101	9	0.275846362906492
1101	10-11	0.25476362003026054
1101	12-13	0.2729747055949048
1101	14-15	0.38088957634677456
1101	16-17	0.3590768794910417
1101	18-19	0.33877822585804296
1101	20-21	0.32268072942446224
1101	22-23	0.3622363420378889
1101	24-25	0.32771178814029867
1101	26-27	0.37630059507758773
1101	28-29	0.42978563707804085
1101	30-31	0.45896865663640085
1101	32-33	0.3649101805649906
1101	34-35	0.3929043753635071
1101	36-37	0.3470528636051142
1101	38-39	0.37958429588366016
1101	40-41	0.3957144822148564
1101	42-43	0.36992981903880917
1101	44-45	0.3828226545819362
1101	46-47	0.4418352267671395
1101	48-49	0.4639858497700473
1101	50-51	0.4708366944268647
1101	52-53	0.45225964310498057
1101	54-55	0.4115484778564422
1101	56-57	0.42041876808441003
1101	58-59	0.3736125958231895
1101	60-61	0.3409736012554845
1101	62-63	0.31554449778580107
1101	64-65	0.34630564149070864
1101	66-67	0.2513968792428187
1101	68-69	0.29066824649934375
1101	70-71	0.19549926342754276
1101	72-73	0.22318041332789562
1101	74-75	0.2625748854588963
1101	76-77	0.27488175805925863
1101	78-79	0.23150052355993722
1101	80-81	0.2940167155199447
1101	82-83	0.28273957180237375
1101	84-85	0.2729719508759061
1101	86-87	0.2848735222321537
1101	88-89	0.25079203498544445
1101	90-91	0.24393888306348543
1101	92-93	0.21073316014476973
1101	94-95	0.22570544824517924
1101	96-97	0.20338132944600318
1101	98-99	0.19114874607406307
1101	100	0.2195487072015112
1102	1	0.2338197257767476
1102	2	0.2843845574877477
1102	3	0.2641435352660153
1102	4	0.22031491652779778
1102	5	0.15585914259237654
1102	6	0.16959103539626597
1102	7	0.19811742659176446
1102	8	0.18359781322354962
1102	9	0.20334364092197177
1102	10-11	0.2234115828575085
1102	12-13	0.23228677304966538
1102	14-15	0.354808286496592
1102	16-17	0.33702964916119527
1102	18-19	0.3072567413003
1102	20-21	0.3223360241784903
1102	22-23	0.361360450030503
1102	24-25	0.3047469992274472
1102	26-27	0.33693561355489976
1102	28-29	0.4163091567922379
1102	30-31	0.3908318865737215
1102	32-33	0.3753776288880104
1102	34-35	0.36662061277339575
1102	36-37	0.417758906957026
1102	38-39	0.38324981230783806
1102	40-41	0.3924590761380742
1102	42-43	0.2719199020958385
1102	44-45	0.3287452499040313
1102	46-47	0.36111837890963017
1102	48-49	0.3829555341827344
1102	50-51	0.3310078879343905
1102	52-53	0.2895683021444597
1102	54-55	0.22623968532500527
1102	56-57	0.17817118459681325
1102	58-59	0.18439300532170222
1102	60-61	0.15388049597621745
1102	62-63	0.10891845942394696
1102	64-65	0.1510212549448653
1102	66-67	0.11183962559358918
1102	68-69	0.1445540941862049
1102	70-71	0.13410517827357893
1102	72-73	0.150482816918192
1102	74-75	0.11017111637694654
1102	76-77	0.06014921178552868
1102	78-79	0.10414293534648067
1102	80-81	0.1150693016187816
1102	82-83	0.15215771570392178
1102	84-85	0.17620701532053573
1102	86-87	0.17438752011575076
1102	88-89	0.17545891592789786
1102	90-91	0.12409920651132467
1102	92-93	0.15539801089697391
1102	94-95	0.17485990976702226
1102	96-97	0.17375386140908944
1102	98-99	0.179835352042943
1102	100	0.16522552435586135
1103	1	0.15891116493374113
1103	2	0.2148434154582617
1103	3	0.22316649237935593
1103	4	0.11238098470659708
1103	5	0.08960152771678054
1103	6	0.10828178663210508
1103	7	0.12285719126268191
1103	8	0.16443491790484188
1103	9	0.18886395806014633
1103	10-11	0.1579171613189203
1103	12-13	0.187803456881511
1103	14-15	0.2578552033414496
1103	16-17	0.24555571325370096
1103	18-19	0.2582417899699294
1103	20-21	0.2347010355711916
1103	22-23	0.28217735261885224
1103	24-25	0.2816247097554623
1103	26-27	0.2580327375001161
1103	28-29	0.33080553732165896
1103	30-31	0.3770301081678795
1103	32-33	0.36483549674937166
1103	34-35	0.351598729541827
1103	36-37	0.315803820460836
1103	38-39	0.365477789107743
1103	40-41	0.4357746453769096
1103	42-43	0.37194608578231936
1103	44-45	0.39833510136112693
1103	46-47	0.47062186305518594
1103	48-49	0.48286387706527023
1103	50-51	0.4924189431928738
1103	52-53	0.534953273409684
1103	54-55	0.5254035131354726
1103	56-57	0.4729976463629342
1103	58-59	0.5055967593212785
1103	60-61	0.4420836127730894
1103	62-63	0.41487151907726627
1103	64-65	0.40743763222952367
1103	66-67	0.4410407758183936
1103	68-69	0.4499678438152017
1103	70-71	0.36882558047319947
1103	72-73	0.42453484995872515
1103	74-75	0.3708807464013617
1103	76-77	0.3169517107876416
1103	78-79	0.3411437253110492
1103	80-81	0.3505968209291339
1103	82-83	0.3612284345906289
1103	84-85	0.3027058252563535
1103	86-87	0.3656282193632734
1103	88-89	0.3686835293133939
1103	90-91	0.3628717308309817
1103	92-93	0.37487719529267594
1103	94-95	0.27549197618772325
1103	96-97	0.30028451252714916
1103	98-99	0.31874933271004124
1103	100	0.24005486130494091
1104	1	0.16455803177284167
1104	2	0.1903124477188669
1104	3	0.22858419647999284
1104	4	0.1547336040849956
1104	5	0.13114948355295297
1104	6	0.12066355136050788
1104	7	0.16500005786134864
1104	8	0.15182667384241455
1104	9	0.20286122170320198
1104	10-11	0.15844431611951393
1104	12-13	0.19297585309368515
1104	14-15	0.25334377436418265
1104	16-17	0.2972311952020874
1104	18-19	0.26552186619292684
1104	20-21	0.23459665113880135
1104	22-23	0.2645870551652578
1104	24-25	0.2690045692667127
1104	26-27	0.3121296788841903
1104	28-29	0.32254995414095333
1104	30-31	0.3577743110303899
1104	32-33	0.36507007102010647
1104	34-35	0.3264871178068418
1104	36-37	0.3951247187787672
1104	38-39	0.3542197447084945
1104	40-41	0.3676471214114301
1104	42-43	0.31315112936616885
1104	44-45	0.33105803770683906
1104	46-47	0.43957885416453024
1104	48-49	0.43306525762317705
1104	50-51	0.43616034064550036
1104	52-53	0.4466356803304379
1104	54-55	0.34153914599136215
1104	56-57	0.34005691596888
1104	58-59	0.3880425804913301
1104	60-61	0.35624503815458297
1104	62-63	0.37912047711132857
1104	64-65	0.3635883887527811
1104	66-67	0.32086710230571214
1104	68-69	0.3161502662997009
1104	70-71	0.3013131822502544
1104	72-73	0.23138652960889772
1104	74-75	0.19951612433382593
1104	76-77	0.25009044065003394
1104	78-79	0.28084990862620884
1104	80-81	0.2903790787599334
1104	82-83	0.3510100305150061
1104	84-85	0.2802809274851157
1104	86-87	0.2874870350802965
1104	88-89	0.3177882919269237
1104	90-91	0.34077563954942747
1104	92-93	0.3041514202628619
1104	94-95	0.2893765368446495
1104	96-97	0.2628559850041867
1104	98-99	0.28550928644099827
1104	100	0.224333420867751
1105	1	0.08350573764280966
1105	2	0.111420222487574
1105	3	0.14330234248848228
1105	4	0.10606300471608421
1105	5	0.12765760494755085
1105	6	0.10485084206948159
1105	7	0.05317539757199796
1105	8	0.14908140205612597
1105	9	0.12353213974777333
1105	10-11	0.1397102204604579
1105	12-13	0.12702652070514375
1105	14-15	0.1227555971154004
1105	16-17	0.12297354378382863
1105	18-19	0.13438675027590108
1105	20-21	0.18269884090172184
1105	22-23	0.1978199180635727
1105	24-25	0.1316856497259522
1105	26-27	0.1312186400536035
1105	28-29	0.18059110544933077
1105	30-31	0.16655738736949388
1105	32-33	0.16216913080295114
1105	34-35	0.14151903867809068
1105	36-37	0.10346401281796602
1105	38-39	0.15979647781523454
1105	40-41	0.10857389402617201
1105	42-43	0.06527117785842762
1105	44-45	0.06621831809183476
1105	46-47	0.09069925612560326
1105	48-49	0.08135373545682967
1105	50-51	0.10406146226645774
1105	52-53	0.06211399505521342
1105	54-55	0.09297490131754671
1105	56-57	0.11759403412110458
1105	58-59	0.16319012707306513
1105	60-61	0.11552855345783541
1105	62-63	0.07064334923850168
1105	64-65	0.13633305806356333
1105	66-67	0.13720475866938386
1105	68-69	0.14488305039685656
1105	70-71	0.060251575782793054
1105	72-73	0.09318327685273431
1105	74-75	0.06678155578521228
1105	76-77	0.1352201799070727
1105	78-79	0.06381992608973519
1105	80-81	0.07390962984650429
1105	82-83	0.0805874125432311
1105	84-85	0.10012934663154027
1105	86-87	0.09362719435366529
1105	88-89	0.09747072700563564
1105	90-91	0.05918871245797419
1105	92-93	0.0011310758468709992
1105	94-95	-0.004387296228472337
1105	96-97	0.0076321132883308
1105	98-99	0.05656004091139266
1105	100	0.07484776056988807
1106	1	0.10346299634228018
1106	2	0.07854460259605389
1106	3	0.12826837416647408
1106	4	0.1208307536388844
1106	5	0.11329519805065758
1106	6	0.1097550325608978
1106	7	0.08724322763394099
1106	8	0.09081566699229882
1106	9	0.07233265511457887
1106	10-11	0.10926971813816522
1106	12-13	0.10028785925816663
1106	14-15	0.18026393916747452
1106	16-17	0.15874539022168932
1106	18-19	0.13349330005126347
1106	20-21	0.16543219022237565
1106	22-23	0.1527856880023748
1106	24-25	0.1942423746189803
1106	26-27	0.17725844374450617
1106	28-29	0.22689771804208902
1106	30-31	0.24087893465833332
1106	32-33	0.19257414506537884
1106	34-35	0.15077352296583513
1106	36-37	0.16047446092723305
1106	38-39	0.10064920048040449
1106	40-41	0.12211158670806554
1106	42-43	0.1992481537711086
1106	44-45	0.20507684759396483
1106	46-47	0.21460752668280492
1106	48-49	0.22665128456416994
1106	50-51	0.19711646074200928
1106	52-53	0.17315259733969413
1106	54-55	0.1816448253400864
1106	56-57	0.15559809131417524
1106	58-59	0.2064080994527373
1106	60-61	0.11783539524923725
1106	62-63	0.11721948244534985
1106	64-65	0.20400336479122672
1106	66-67	0.1807838603758114
1106	68-69	0.23887347168610518
1106	70-71	0.1457914765058348
1106	72-73	0.11742857786811811
1106	74-75	0.11029699120484793
1106	76-77	0.1664332844096137
1106	78-79	0.1487480409569244
1106	80-81	0.17668081108956812
1106	82-83	0.12246391268011081
1106	84-85	0.10461605134235441
1106	86-87	0.04818302414584963
1106	88-89	0.06586300942552015
1106	90-91	0.06912867043350701
1106	92-93	-0.03964034261205995
1106	94-95	0.007730166655846915
1106	96-97	0.03593814439567211
1106	98-99	0.07011989889313952
1106	100	0.11724078293349294
1107	1	0.04275880172237123
1107	2	0.060115123404116844
1107	3	0.09254715008309944
1107	4	0.01874679035385185
1107	5	-0.0036874136210869324
1107	6	0.08079597305247432
1107	7	0.04129214179104679
1107	8	0.08427103650673473
1107	9	0.09223724581488568
1107	10-11	0.054509941564731434
1107	12-13	0.04203845487403868
1107	14-15	0.08000885718791295
1107	16-17	0.07297382538290265
1107	18-19	0.07969559124788361
1107	20-21	0.04115543015117851
1107	22-23	0.08075354966664605
1107	24-25	0.11433182499096972
1107	26-27	0.10083342377988203
1107	28-29	0.11324623556499347
1107	30-31	0.13265871572865962
1107	32-33	0.052579217907172904
1107	34-35	0.08284595605804412
1107	36-37	0.16350465319679586
1107	38-39	0.12490657742245759
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2216	26-27	-0.4091714614127966
2216	28-29	-0.41948095829516063
2216	30-31	-0.4409757909237513
2216	32-33	-0.4459289071971355
2216	34-35	-0.46885752676430315
2216	36-37	-0.4848556862328408
2216	38-39	-0.4534847103772819
2216	40-41	-0.42913605891841655
2216	42-43	-0.4947629102410005
2216	44-45	-0.4645537398021915
2216	46-47	-0.4102371277147938
2216	48-49	-0.40520683792686896
2216	50-51	-0.3796511348173084
2216	52-53	-0.3907629717267156
2216	54-55	-0.38766161333336413
2216	56-57	-0.4024760678318131
2216	58-59	-0.3538672677747172
2216	60-61	-0.38714089643023897
2216	62-63	-0.4061774132283489
2216	64-65	-0.31592552768854176
2216	66-67	-0.3415678005964011
2216	68-69	-0.39011004081963563
2216	70-71	-0.3363840811755381
2216	72-73	-0.30924599996973967
2216	74-75	-0.3438765751736099
2216	76-77	-0.3183218477329106
2216	78-79	-0.39018704164887197
2216	80-81	-0.39518277746264374
2216	82-83	-0.3908838078223269
2216	84-85	-0.36299288520255857
2216	86-87	-0.34705068346760726
2216	88-89	-0.3062548019108817
2216	90-91	-0.2335651254375648
2216	92-93	-0.28828574556990105
2216	94-95	-0.26507113487799217
2216	96-97	-0.25568156215014426
2216	98-99	-0.31672610131162315
2216	100	-0.40917352025139664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	19.0
8	75.0
9	120.0
10	210.0
11	221.0
12	163.0
13	175.0
14	300.0
15	542.0
16	1075.0
17	1951.0
18	3662.0
19	5428.0
20	8174.0
21	11927.0
22	17568.0
23	20795.0
24	20325.0
25	17402.0
26	15566.0
27	15584.0
28	18127.0
29	20308.0
30	25168.0
31	31813.0
32	41548.0
33	54717.0
34	81395.0
35	131972.0
36	243333.0
37	600222.0
38	837568.0
39	303625.0
40	745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82531757568097	19.800330215509067	13.563325538772672	23.81102667003729
2	15.552840948399401	20.862454413378007	39.547464444905664	24.03724019331693
3	17.59330756789102	26.555929244435315	31.22799256659748	24.622770621076185
4	12.365398371055164	18.00805190568219	38.55759269111624	31.0689570321464
5	12.62431114958747	38.193300503744396	35.33417863663825	13.848209710029883
6	29.397737888103077	38.56152276825301	18.001741909108684	14.038997434535224
7	25.304306542877026	33.80841939125263	23.241286314756724	17.645987751113626
8	25.77956673906509	34.196426843424675	20.91943236158294	19.10457405592729
9	26.561730421123436	14.494378161506551	20.896050000335727	38.04784141703429
10-11	24.36027321025206	26.05318381261249	28.689979512785847	20.8965634643496
12-13	25.649344365700127	24.333750819073845	28.393414547541436	21.623490267684588
14-15	22.798873143753166	25.065876458559	26.960460300830725	25.17479009685711
16-17	21.46449416092673	27.905663231592413	27.713094477773524	22.91674812970733
18-19	21.36831840140484	26.452777307102433	30.67592007814132	21.502984213351407
20-21	22.499716607361574	25.708333481447955	30.342918916527733	21.44903099466274
22-23	22.39525383213834	25.572801445289556	30.13265998133352	21.899284741238585
24-25	22.12943005889432	25.736593750826977	30.460423181241342	21.67355300903736
26-27	21.973356747292367	25.909078162257	30.088063028102674	22.029502062347962
28-29	21.696603928592943	26.171423944609074	30.153199918240713	21.978772208557274
30-31	22.14360505233589	25.78098987328873	30.113922045770252	21.961483028605127
32-33	22.1484664137219	26.09608741366739	29.669465490260205	22.085980682350506
34-35	22.304792261601474	26.03563091725617	29.99352236954882	21.666054451593535
36-37	22.48696778026086	25.686665070965265	29.747073428332865	22.079293720441015
38-39	22.32814138529626	25.769421420686033	29.801209018862036	22.10122817515567
40-41	22.22602498275427	25.644520183672675	29.911270164368126	22.218184669204927
42-43	22.36553045264239	25.732285330594834	29.427936645864598	22.474247570898175
44-45	22.253995707814624	26.09363931463275	29.437827199152544	22.214537778400082
46-47	22.6824979940284	26.109406471364128	28.61270894344394	22.595386591163535
48-49	22.83340538347892	26.28430783376975	28.277118091603942	22.60516869114739
50-51	22.742878742366855	26.425905485951024	28.11290449673523	22.71831127494689
52-53	22.685722794677588	26.621623329636336	27.979519579869372	22.713134295816705
54-55	22.40704783105681	26.627882451360424	27.97643639707306	22.98863332050971
56-57	22.932591199497114	26.592143885068264	27.63995633933743	22.83530857609719
58-59	22.401726708386104	26.49647763891706	28.191784863731794	22.91001078896504
60-61	22.46838436631971	26.759124765110677	27.98541735993783	22.78707350863178
62-63	22.55279357135945	26.509591021389962	27.86821207878205	23.06940332846854
64-65	22.23281890592324	26.52779371449664	27.967707259250602	23.271680120329524
66-67	22.517198870169416	26.571953231554836	27.778846446290984	23.132001451984767
68-69	22.484315576118558	26.30587011300369	27.972180304270022	23.23763400660773
70-71	22.45705046588773	26.201136394381898	27.848357252280458	23.493455887449915
72-73	22.709194662710427	26.26969183121045	27.824775239691007	23.19633826638812
74-75	22.64094378245684	26.729140478191276	27.53566487704542	23.094250862306467
76-77	22.783277426184295	26.37307925122273	27.43600960213925	23.40763372045372
78-79	22.605899396141442	26.44336453706975	27.438308255998745	23.512427810790065
80-81	22.614682232795197	26.59173907170409	27.47430506567997	23.31927362982074
82-83	22.81554653919566	26.18427276057615	27.84323909602489	23.156941604203304
84-85	22.914894638067747	26.527769247464423	27.293818815354403	23.263517299113424
86-87	22.601579968267924	26.653640479606988	27.311861848162373	23.43291770396272
88-89	22.78241454069189	26.30567192994444	27.228940931401496	23.682972597962177
90-91	22.60333759508465	26.23021040570372	27.418069904570736	23.748382094640895
92-93	22.819051726759728	26.3050576600339	27.316147297816634	23.559743315389742
94-95	22.349172908216726	26.2077957266365	27.591206020326066	23.85182534482071
96-97	22.57723118680914	25.988954954826855	27.514027491750976	23.91978636661303
98-99	22.444125436767656	26.275535343397678	27.28074525354297	23.999593966291698
100	22.606423001437314	25.89707667290595	27.268152746240137	24.2283475794166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	467.0
1	310.0
2	359.0
3	521.5
4	321.0
5	262.0
6	579.5
7	1388.5
8	2653.0
9	3226.0
10	2323.0
11	1428.0
12	1395.5
13	1540.0
14	1800.5
15	2142.0
16	2418.0
17	2754.0
18	3221.0
19	3712.5
20	4277.0
21	4926.0
22	5811.5
23	6933.5
24	8248.5
25	10313.5
26	13215.0
27	16758.5
28	20486.0
29	24996.5
30	30007.0
31	34865.0
32	41587.0
33	46237.5
34	49768.5
35	53958.5
36	58318.0
37	65115.0
38	68762.0
39	69180.0
40	70408.5
41	71665.0
42	72785.0
43	75911.0
44	80721.5
45	88986.5
46	95329.0
47	98339.5
48	101436.5
49	100287.0
50	97547.5
51	97665.0
52	97182.0
53	94455.0
54	91664.5
55	84811.0
56	76891.0
57	68322.0
58	59828.0
59	54100.5
60	45445.5
61	36159.0
62	28755.5
63	21420.5
64	14898.0
65	10306.5
66	7522.0
67	5376.5
68	3822.0
69	2862.0
70	2271.5
71	1824.5
72	1404.0
73	1245.5
74	1058.0
75	832.5
76	658.5
77	458.5
78	289.5
79	183.5
80	124.0
81	75.0
82	50.0
83	34.5
84	22.0
85	13.5
86	8.0
87	5.0
88	3.0
89	1.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005648104152620464
2	0.09076463876029248
3	3.159778546920539E-4
4	0.0
5	0.038549298272430574
6	0.004660673356707795
7	3.949723183650674E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.974861591825337E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.7396678203808087E-4
24-25	0.0
26-27	0.0
28-29	1.974861591825337E-5
30-31	1.5798892734602695E-4
32-33	0.0017773754326428032
34-35	0.0013034086506047223
36-37	8.294418685666416E-4
38-39	7.109501730571213E-4
40-41	0.001441648962032496
42-43	9.084363322396549E-4
44-45	4.937153979563342E-4
46-47	2.9622923877380055E-4
48-49	8.491904844848949E-4
50-51	7.701960208118815E-4
52-53	0.001540392041623763
54-55	0.0014613975779507495
56-57	9.676821799944151E-4
58-59	0.0027450576126372183
60-61	0.0017971240485610566
62-63	0.0011059224914221888
64-65	0.004285449654260981
66-67	0.004581678893034782
68-69	0.0036139967130403663
70-71	0.0017181295848880433
72-73	0.004976651211399849
74-75	0.003771985640386394
76-77	0.003771985640386394
78-79	8.491904844848949E-4
80-81	3.949723183650674E-5
82-83	2.1723477510078706E-4
84-85	9.874307959126686E-5
86-87	0.0
88-89	1.5798892734602695E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.5547508652856064E-4
98-99	5.332126297928411E-4
100	6.319557093841078E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2531823.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.581185549858766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.653292627414	35.57290332700948
2	16.403236202704228	17.250032127851036
3	6.658876248645806	10.50394822750778
4	3.3271854028511503	6.997894121043917
5	1.8947620195210606	4.9814416660631
6	1.1523667819591032	3.635568695021115
7	0.7440336076795022	2.738551842650871
8	0.5009494574522606	2.107241309871872
9	0.36268784466849285	1.7163501171473143
>10	1.2628761030726947	10.163927478030118
>50	0.025810345797220635	0.9066365491532169
>100	0.011719051778889156	1.0707410073121606
>500	0.0015082096801204347	0.5547581553324393
>1k	4.640645169601338E-4	0.6265582816603787
>5k	1.1601612924003345E-4	0.548291873776992
>10k+	1.1601612924003345E-4	0.625155220568159
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10248	0.40476763186052106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8988	0.35500111974652254	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4901	0.19357593323071953	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	2610	0.10308777509328258	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.949723183650674E-5
2	0.0	0.0	0.0	0.0	3.949723183650674E-5
3	0.0	0.0	0.0	0.0	3.949723183650674E-5
4	0.0	0.0	0.0	0.0	3.949723183650674E-5
5	0.0	0.0	0.0	0.0	3.949723183650674E-5
6	0.0	0.0	0.0	0.0	3.949723183650674E-5
7	0.0	0.0	0.0	0.0	3.949723183650674E-5
8	0.0	0.0	0.0	0.0	3.949723183650674E-5
9	0.0	0.0	0.0	0.0	3.949723183650674E-5
10-11	0.0	0.0	0.0	0.0	3.949723183650674E-5
12-13	0.0	0.0	0.0	0.0	7.899446367301347E-5
14-15	0.0	0.0	0.0	0.0	7.899446367301347E-5
16-17	0.0	0.0	0.0	3.949723183650674E-5	7.899446367301347E-5
18-19	0.0	0.0	0.0	7.899446367301347E-5	1.3824031142777358E-4
20-21	0.0	0.0	0.0	1.7773754326428032E-4	1.5798892734602695E-4
22-23	0.0	0.0	0.0	4.739667820380808E-4	1.5798892734602695E-4
24-25	0.0	0.0	0.0	0.0011454197232586955	1.5798892734602695E-4
26-27	0.0	0.0	0.0	0.002567320069372938	1.5798892734602695E-4
28-29	0.0	0.0	0.0	0.008294418685666416	1.5798892734602695E-4
30-31	0.0	0.0	0.0	0.023244120935784214	1.5798892734602695E-4
32-33	0.0	0.0	0.0	0.050951429069093696	1.5798892734602695E-4
34-35	0.0	0.0	0.0	0.09692620692678754	1.5798892734602695E-4
36-37	0.0	0.0	0.0	0.16527616661986244	1.5798892734602695E-4
38-39	0.0	0.0	0.0	0.25608030261199144	1.5798892734602695E-4
40-41	0.0	0.0	0.0	0.3551393600579503	1.5798892734602695E-4
42-43	0.0	0.0	0.0	0.4502881915520951	1.5798892734602695E-4
44-45	0.0	0.0	0.0	0.5654226223555122	1.974861591825337E-4
46-47	0.0	0.0	0.0	0.7010758650979947	2.3698339101904043E-4
48-49	0.0	0.0	0.0	0.8409948088788197	2.3698339101904043E-4
50-51	0.0	0.0	0.0	0.987707276535524	2.3698339101904043E-4
52-53	0.0	3.949723183650674E-5	0.0	1.128001444018796	2.3698339101904043E-4
54-55	0.0	3.949723183650674E-5	0.0	1.2745954199799907	2.3698339101904043E-4
56-57	0.0	3.949723183650674E-5	0.0	1.4431893540741196	2.567320069372938E-4
58-59	0.0	3.949723183650674E-5	0.0	1.6257850568542904	2.7648062285554715E-4
60-61	0.0	3.949723183650674E-5	0.0	1.8150755404307488	2.7648062285554715E-4
62-63	0.0	3.949723183650674E-5	0.0	2.002173927640281	2.7648062285554715E-4
64-65	0.0	3.949723183650674E-5	0.0	2.1932417866493825	3.159778546920539E-4
66-67	0.0	3.949723183650674E-5	0.0	2.4050259437567316	3.159778546920539E-4
68-69	0.0	3.949723183650674E-5	0.0	2.6380596115921215	3.159778546920539E-4
70-71	0.0	3.949723183650674E-5	0.0	2.881757532023368	3.159778546920539E-4
72-73	0.0	3.949723183650674E-5	0.0	3.128773219928881	3.159778546920539E-4
74-75	0.0	3.949723183650674E-5	0.0	3.376203628768678	3.159778546920539E-4
76-77	0.0	3.949723183650674E-5	0.0	3.6394329303430766	3.159778546920539E-4
78-79	0.0	3.949723183650674E-5	0.0	3.9288883938569166	3.159778546920539E-4
80-81	0.0	3.949723183650674E-5	0.0	4.230844731247011	3.159778546920539E-4
82-83	0.0	3.949723183650674E-5	0.0	4.542813616907659	3.159778546920539E-4
84-85	0.0	3.949723183650674E-5	0.0	4.859443175925016	3.159778546920539E-4
86-87	0.0	3.949723183650674E-5	0.0	5.178027847918279	3.75223702446814E-4
88	0.0	3.949723183650674E-5	0.0	5.430987869215186	4.344695502015741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5415	0.0	59.874523	1
GTATCAA	10505	0.0	43.629894	1
CAACGCA	13295	0.0	33.91494	5
TATCAAC	13485	0.0	33.81314	2
ATCAACG	13345	0.0	33.752636	3
TCAACGC	14130	0.0	32.043865	4
AACGCAG	14275	0.0	31.90276	6
ACGCAGA	15565	0.0	29.046778	7
CGCAGAG	15785	0.0	28.641949	8
GCAGAGT	16940	0.0	26.328423	9
GTGGTAT	1875	0.0	24.59496	1
GTACATG	8325	0.0	23.514208	1
TACATGG	8360	0.0	23.077583	2
GAGTACT	12475	0.0	22.660376	12-13
ACATGGG	8300	0.0	22.602451	3
AGAGTAC	16070	0.0	22.504261	10-11
CAGAGTA	16800	0.0	22.253736	10-11
TGGTATC	1945	0.0	21.531939	2
GTACTAG	1130	0.0	21.23797	1
GTACTTT	13220	0.0	21.205624	14-15
>>END_MODULE