FastQCFastQC Report
Mon 16 Jan 2017
ERR1146503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723909
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.039.8651541
GTATCAA69300.035.4825741
CAACGCA71200.032.604525
ATCAACG71600.032.553643
AACGCAG78350.030.1689266
TATCAAC81050.029.9756322
TCAACGC80950.029.3160444
ACGCAGA89100.025.9474667
GTACATG122500.025.9452151
GTACTAG17400.025.3995061
CGCAGAG90500.025.3393968
TACATGG123750.024.7590072
ACATGGG124100.024.1211783
CTAACGC6150.023.6874033
CATGGGG85900.022.2107734
TCTAACG6900.021.1126842
GCATCGT13650.021.0001418
GCAGAGT106800.020.8120529
TGATCGC10350.019.9773259
TAACGCC6700.019.6387624
Produced by FastQC (version 0.11.5)